This is a Validated Antibody Database (VAD) review about human KEAP1, based on 30 published articles (read how Labome selects the articles), using KEAP1 antibody in all methods. It is aimed to help Labome visitors find the most suited KEAP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
KEAP1 synonym: INrf2; KLHL19

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (G-2)
  • western blot knockout validation; human; loading ...; fig 1c
Santa Cruz Biotechnology KEAP1 antibody (Santa Cruz Biotechnology, sc-365626) was used in western blot knockout validation on human samples (fig 1c). iScience (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-2)
  • western blot knockout validation; human; 1:500; loading ...; fig 5a
Santa Cruz Biotechnology KEAP1 antibody (Santa Cruz, sc-365626) was used in western blot knockout validation on human samples at 1:500 (fig 5a). Cell Rep (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D6B12)
  • western blot knockout validation; human; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Tech, 8047) was used in western blot knockout validation on human samples (fig 6a). Animals (Basel) (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D6B12)
  • western blot knockout validation; human; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Tech, 8047) was used in western blot knockout validation on human samples (fig 6a). BMC Cancer (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D6B12)
  • western blot knockout validation; human; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Tech, 8047) was used in western blot knockout validation on human samples (fig 6a). Aging (Albany NY) (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-2)
  • western blot knockout validation; human; loading ...; fig 1c
Santa Cruz Biotechnology KEAP1 antibody (Santa Cruz Biotechnology, sc-365626) was used in western blot knockout validation on human samples (fig 1c). iScience (2021) ncbi
mouse monoclonal (G-2)
  • immunoprecipitation; human; 1:200; loading ...; fig 2d
Santa Cruz Biotechnology KEAP1 antibody (Santa, sc-365626) was used in immunoprecipitation on human samples at 1:200 (fig 2d). Atherosclerosis (2019) ncbi
mouse monoclonal (G-2)
  • western blot knockout validation; human; 1:500; loading ...; fig 5a
Santa Cruz Biotechnology KEAP1 antibody (Santa Cruz, sc-365626) was used in western blot knockout validation on human samples at 1:500 (fig 5a). Cell Rep (2019) ncbi
mouse monoclonal (G-2)
  • western blot; human; 1:1000; loading ...; fig 1a
Santa Cruz Biotechnology KEAP1 antibody (Santa Cruz Biotechnology, sc-365626) was used in western blot on human samples at 1:1000 (fig 1a). Int J Mol Sci (2017) ncbi
mouse monoclonal (G-2)
  • western blot; human; 1:300-500
In order to elucidate the role of Nrf2 at the blood brain barrier, Santa Cruz Biotechnology KEAP1 antibody (Santa Cruz, sc-365626) was used in western blot on human samples at 1:300-500. PLoS ONE (2015) ncbi
OriGene
mouse monoclonal (OTI1G2)
  • immunocytochemistry; human; loading ...; fig 8a
  • western blot; human; 1:2000; loading ...; fig s11c
OriGene KEAP1 antibody (Origene, TA501989) was used in immunocytochemistry on human samples (fig 8a) and in western blot on human samples at 1:2000 (fig s11c). Nat Commun (2019) ncbi
mouse monoclonal (OTI1G2)
  • immunohistochemistry - paraffin section; human; 1:100; fig 1b
OriGene KEAP1 antibody (Origene, 1G2) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1b). Int J Clin Exp Pathol (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 1e
  • western blot; human; loading ...; fig 1d
Abcam KEAP1 antibody (Abcam, ab226997) was used in immunocytochemistry on human samples (fig 1e) and in western blot on human samples (fig 1d). Sci Adv (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D6B12)
  • western blot; mouse; loading ...; fig 3a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Technology, 8047) was used in western blot on mouse samples (fig 3a). Oxid Med Cell Longev (2022) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4h
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 4678) was used in western blot on mouse samples (fig 4h). Sci Adv (2022) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Cell Signaling Technology KEAP1 antibody (CST, 8047S) was used in western blot on mouse samples at 1:1000 (fig 4b). Front Pharmacol (2021) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; 1:1000; loading ...; fig 5j, 5m
Cell Signaling Technology KEAP1 antibody (CST, 8047) was used in western blot on human samples at 1:1000 (fig 5j, 5m). J Cell Mol Med (2021) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig 6c
Cell Signaling Technology KEAP1 antibody (Cell Signaling Technology, 4678) was used in western blot on rat samples at 1:1000 (fig 6c). Front Pharmacol (2021) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot knockout validation; human; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Tech, 8047) was used in western blot knockout validation on human samples (fig 6a). Animals (Basel) (2021) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot knockout validation; human; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Tech, 8047) was used in western blot knockout validation on human samples (fig 6a). BMC Cancer (2021) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot knockout validation; human; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling Tech, 8047) was used in western blot knockout validation on human samples (fig 6a). Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; 1:200; fig 3j
Cell Signaling Technology KEAP1 antibody (Cell Signaling Technology, 8047) was used in western blot on human samples at 1:200 (fig 3j). Mol Oncol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...
Cell Signaling Technology KEAP1 antibody (Cell Signaling Technology, 4617) was used in western blot on human samples . Nat Commun (2020) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; mouse; 1:1000; loading ...; fig 8b
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047) was used in western blot on mouse samples at 1:1000 (fig 8b). Front Pharmacol (2019) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; loading ...; fig s2k
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047) was used in western blot on human samples (fig s2k). Cell (2019) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; mouse; 1:1000; loading ...; fig 3i
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047) was used in western blot on mouse samples at 1:1000 (fig 3i). Biochem Biophys Res Commun (2018) ncbi
domestic rabbit monoclonal (D1G10)
  • western blot; human; loading ...; fig 1e
Cell Signaling Technology KEAP1 antibody (Cell signaling, D1G10) was used in western blot on human samples (fig 1e). Science (2018) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; 1:1000; loading ...; fig 2f, 2h
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047S) was used in western blot on human samples at 1:1000 (fig 2f, 2h). EMBO J (2018) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; loading ...; fig 1A; 1B
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047) was used in western blot on human samples (fig 1A; 1B). Oncotarget (2017) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; 1:1000; loading ...; fig 2f
In order to identify and characterize an inhibitor of the COP9 signalosome, Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047) was used in western blot on human samples at 1:1000 (fig 2f). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; rat; fig 3
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047) was used in western blot on rat samples (fig 3). Sci Rep (2016) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; human; fig 2a
Cell Signaling Technology KEAP1 antibody (Cell-Signaling, 8047) was used in western blot on human samples (fig 2a). Free Radic Biol Med (2015) ncbi
domestic rabbit monoclonal (D1G10)
  • western blot; human; fig s7
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 7705S) was used in western blot on human samples (fig s7). Mol Cancer Res (2015) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; mouse; fig 6a
Cell Signaling Technology KEAP1 antibody (Cell Signaling, D6B12) was used in western blot on mouse samples (fig 6a). J Biol Chem (2015) ncbi
domestic rabbit monoclonal (D6B12)
  • western blot; rat; 1:1000
Cell Signaling Technology KEAP1 antibody (Cell Signaling, 8047S) was used in western blot on rat samples at 1:1000. PLoS ONE (2014) ncbi
Articles Reviewed
  1. Wang W, Ma P, Gao W, Lu P, Ding X, Chen J, et al. Nrf2 Knockout Affected the Ferroptosis Signaling Pathway against Cisplatin-Induced Hair Cell-Like HEI-OC1 Cell Death. Oxid Med Cell Longev. 2022;2022:2210733 pubmed publisher
  2. Kumar B, Adebayo A, Prasad M, Capitano M, Wang R, Bhat Nakshatri P, et al. Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity. Sci Adv. 2022;8:eabh3375 pubmed publisher
  3. Li S, Jin H, Sun G, Zhang C, Wang J, Xu H, et al. Dietary Inorganic Nitrate Protects Hepatic Ischemia-Reperfusion Injury Through NRF2-Mediated Antioxidative Stress. Front Pharmacol. 2021;12:634115 pubmed publisher
  4. Koppula P, Olszewski K, Zhang Y, Kondiparthi L, Liu X, Lei G, et al. KEAP1 deficiency drives glucose dependency and sensitizes lung cancer cells and tumors to GLUT inhibition. iScience. 2021;24:102649 pubmed publisher
  5. Hu J, Wang J, Li C, Shang Y. Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway. J Cell Mol Med. 2021;25:5001-5014 pubmed publisher
  6. Zhou F, Zou X, Zhang J, Wang Z, Yang Y, Wang D. Jian-Pi-Yi-Shen Formula Ameliorates Oxidative Stress, Inflammation, and Apoptosis by Activating the Nrf2 Signaling in 5/6 Nephrectomized Rats. Front Pharmacol. 2021;12:630210 pubmed publisher
  7. Strous E, Vanhoudt A, Smolenaars A, van Schaik G, Schouten M, de Pater H, et al. Observational Study on Variation of Longitudinal Platelet Counts in Calves over the First 14 Days of Life and Reference Intervals from Cross-Sectional Platelet and Leukocyte Counts in Dairy Calves up to Two Months of Age. Animals (Basel). 2021;11: pubmed publisher
  8. Xi Y, Jing Z, Wei W, Chun Z, Quan Q, Qing Z, et al. Inhibitory effect of sodium butyrate on colorectal cancer cells and construction of the related molecular network. BMC Cancer. 2021;21:127 pubmed publisher
  9. Huang D, Dai C, Li S, Li X. Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury. Aging (Albany NY). 2021;13:11010-11025 pubmed publisher
  10. Mota M, Metge B, Hinshaw D, Alsheikh H, Chen D, Samant R, et al. Merlin deficiency alters the redox management program in breast cancer. Mol Oncol. 2021;15:942-956 pubmed publisher
  11. Brockway S, Wang G, Jackson J, Amici D, Takagishi S, Clutter M, et al. Quantitative and multiplexed chemical-genetic phenotyping in mammalian cells with QMAP-Seq. Nat Commun. 2020;11:5722 pubmed publisher
  12. Yuan Z, Yang L, Zhang X, Ji P, Hua Y, Wei Y. Huang-Lian-Jie-Du Decoction Ameliorates Acute Ulcerative Colitis in Mice via Regulating NF-κB and Nrf2 Signaling Pathways and Enhancing Intestinal Barrier Function. Front Pharmacol. 2019;10:1354 pubmed publisher
  13. Yang Y, Willis T, Button R, Strang C, Fu Y, Wen X, et al. Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response. Nat Commun. 2019;10:3759 pubmed publisher
  14. Sanghvi V, Leibold J, Mina M, Mohan P, Berishaj M, Li Z, et al. The Oncogenic Action of NRF2 Depends on De-glycation by Fructosamine-3-Kinase. Cell. 2019;178:807-819.e21 pubmed publisher
  15. Xu D, Li X, Shao F, Lv G, Lv H, Lee J, et al. The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62. Sci Adv. 2019;5:eaav4570 pubmed publisher
  16. Zhu Y, Zhang Y, Huang X, Xie Y, Qu Y, Long H, et al. Z-Ligustilide protects vascular endothelial cells from oxidative stress and rescues high fat diet-induced atherosclerosis by activating multiple NRF2 downstream genes. Atherosclerosis. 2019;284:110-120 pubmed publisher
  17. Cao J, Poddar A, Magtanong L, Lumb J, Mileur T, Reid M, et al. A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cell Rep. 2019;26:1544-1556.e8 pubmed publisher
  18. Li C, Liu Q, Xie L. Suppressing NLRP2 expression accelerates hepatic steatosis: A mechanism involving inflammation and oxidative stress. Biochem Biophys Res Commun. 2018;507:22-29 pubmed publisher
  19. Mena E, Kjolby R, Saxton R, Werner A, Lew B, Boyle J, et al. Dimerization quality control ensures neuronal development and survival. Science. 2018;362: pubmed publisher
  20. Jena K, Kolapalli S, Mehto S, Nath P, Das B, Sahoo P, et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. EMBO J. 2018;37: pubmed publisher
  21. Skowron M, Niegisch G, Albrecht P, Van Koeveringe G, Romano A, Albers P, et al. Various Mechanisms Involve the Nuclear Factor (Erythroid-Derived 2)-Like (NRF2) to Achieve Cytoprotection in Long-Term Cisplatin-Treated Urothelial Carcinoma Cell Lines. Int J Mol Sci. 2017;18: pubmed publisher
  22. Cheng L, Li K, Yi N, Li X, Wang F, Xue B, et al. miRNA-141 attenuates UV-induced oxidative stress via activating Keap1-Nrf2 signaling in human retinal pigment epithelium cells and retinal ganglion cells. Oncotarget. 2017;8:13186-13194 pubmed publisher
  23. Schlierf A, Altmann E, Quancard J, Jefferson A, Assenberg R, Renatus M, et al. Targeted inhibition of the COP9 signalosome for treatment of cancer. Nat Commun. 2016;7:13166 pubmed publisher
  24. Ashino T, Yamamoto M, Numazawa S. Nrf2/Keap1 system regulates vascular smooth muscle cell apoptosis for vascular homeostasis: role in neointimal formation after vascular injury. Sci Rep. 2016;6:26291 pubmed publisher
  25. Kabaria S, Choi D, Chaudhuri A, Jain M, Li H, Junn E. MicroRNA-7 activates Nrf2 pathway by targeting Keap1 expression. Free Radic Biol Med. 2015;89:548-56 pubmed publisher
  26. Liew P, Hsu C, Liu W, Lee Y, Lee Y, Chen C. Prognostic and predictive values of Nrf2, Keap1, p16 and E-cadherin expression in ovarian epithelial carcinoma. Int J Clin Exp Pathol. 2015;8:5642-9 pubmed
  27. Dorr C, Janik C, Weg M, Been R, Bader J, Kang R, et al. Transposon Mutagenesis Screen Identifies Potential Lung Cancer Drivers and CUL3 as a Tumor Suppressor. Mol Cancer Res. 2015;13:1238-47 pubmed publisher
  28. Sajja R, Green K, Cucullo L. Altered Nrf2 signaling mediates hypoglycemia-induced blood-brain barrier endothelial dysfunction in vitro. PLoS ONE. 2015;10:e0122358 pubmed publisher
  29. Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, et al. Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions. J Biol Chem. 2015;290:9738-52 pubmed publisher
  30. Padiya R, Chowdhury D, Borkar R, Srinivas R, Pal Bhadra M, Banerjee S. Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat. PLoS ONE. 2014;9:e94228 pubmed publisher