This is a Validated Antibody Database (VAD) review about human KDM5B, based on 11 published articles (read how Labome selects the articles), using KDM5B antibody in all methods. It is aimed to help Labome visitors find the most suited KDM5B antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
KDM5B synonym: CT31; JARID1B; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1A; RBP2-H1; lysine-specific demethylase 5B; cancer/testis antigen 31; histone demethylase JARID1B; jumonji, AT rich interactive domain 1B; jumonji/ARID domain-containing protein 1B; lysine (K)-specific demethylase 5B; protein phosphatase 1, regulatory subunit 98; retinoblastoma-binding protein 2 homolog 1; retinoblastoma-binding protein 2, homolog 1A

Knockout validation
Sigma-Aldrich
rabbit polyclonal
  • western blot knockout validation; mouse; fig 1
  • western blot knockout validation; human; fig 1
Sigma-Aldrich KDM5B antibody (Sigma, HPA027179) was used in western blot knockout validation on mouse samples (fig 1) and in western blot knockout validation on human samples (fig 1). Nucleic Acids Res (2016) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig 1a
Bethyl KDM5B antibody (Bethyl, A301-813A) was used in western blot on human samples (fig 1a). Cancer Res (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 6
Bethyl KDM5B antibody (Bethyl, A301-813A) was used in western blot on human samples (fig 6). Cell Rep (2015) ncbi
Bio-Rad
mouse monoclonal (1G10)
  • immunohistochemistry - paraffin section; human; fig 2e
Bio-Rad KDM5B antibody (AbD Serotec, MCA4340Z) was used in immunohistochemistry - paraffin section on human samples (fig 2e). Appl Immunohistochem Mol Morphol (2016) ncbi
Abnova
mouse polyclonal
  • western blot; human; fig 1
Abnova KDM5B antibody (Abnova, H00010765-A01) was used in western blot on human samples (fig 1). BMC Cancer (2016) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; mouse; 1:10,000; fig 1
  • immunocytochemistry; human; 1:250; fig 3
In order to study JARID1B ubiquitination and suppression of prostate tumorigenesis by SKP2 inactivation, Novus Biologicals KDM5B antibody (Novus Biologicals, NB100-97821) was used in western blot on mouse samples at 1:10,000 (fig 1) and in immunocytochemistry on human samples at 1:250 (fig 3). Oncotarget (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; fig 6
Cell Signaling Technology KDM5B antibody (Cell Signaling Technology, 3273) was used in western blot on human samples (fig 6). Cell Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 3k
In order to discover the role of MiR137 and its effect on transciptional coregulators, Cell Signaling Technology KDM5B antibody (Cell Signaling Technology, 3273) was used in western blot on human samples at 1:1000 (fig 3k). Oncotarget (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot knockout validation; mouse; fig 1
  • western blot knockout validation; human; fig 1
Sigma-Aldrich KDM5B antibody (Sigma, HPA027179) was used in western blot knockout validation on mouse samples (fig 1) and in western blot knockout validation on human samples (fig 1). Nucleic Acids Res (2016) ncbi
mouse monoclonal (1G10)
  • western blot; human; 1:4000; fig s1
Sigma-Aldrich KDM5B antibody (Sigma, SAB1404865) was used in western blot on human samples at 1:4000 (fig s1). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4b
Sigma-Aldrich KDM5B antibody (Sigma, HPA027179) was used in western blot on human samples (fig 4b). J Biol Chem (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1e
Sigma-Aldrich KDM5B antibody (Sigma, HPA027179) was used in western blot on mouse samples (fig 1e). J Biol Chem (2014) ncbi
Articles Reviewed
  1. Cao J, Wu L, Zhang S, Lu M, Cheung W, Cai W, et al. An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Nucleic Acids Res. 2016;44:e149 pubmed
  2. Penterling C, Drexler G, Böhland C, Stamp R, Wilke C, Braselmann H, et al. Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair. PLoS ONE. 2016;11:e0156599 pubmed publisher
  3. Huang C, Cheng J, Bawa Khalfe T, Yao X, Chin Y, Yeh E. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Cell Rep. 2016;15:147-157 pubmed publisher
  4. Bamodu O, Huang W, Lee W, Wu A, Wang L, Hsiao M, et al. Aberrant KDM5B expression promotes aggressive breast cancer through MALAT1 overexpression and downregulation of hsa-miR-448. BMC Cancer. 2016;16:160 pubmed publisher
  5. Li N, Dhar S, Chen T, Kan P, Wei Y, Kim J, et al. JARID1D Is a Suppressor and Prognostic Marker of Prostate Cancer Invasion and Metastasis. Cancer Res. 2016;76:831-43 pubmed publisher
  6. Nilsson E, Laursen K, Whitchurch J, McWilliam A, Ødum N, Persson J, et al. MiR137 is an androgen regulated repressor of an extended network of transcriptional coregulators. Oncotarget. 2015;6:35710-25 pubmed publisher
  7. Kuźbicki Å, Lange D, StrÄ…czyÅ„ska Niemiec A, Chwirot B. Altered Splicing of JARID1B in Development of Human Cutaneous Melanoma?. Appl Immunohistochem Mol Morphol. 2016;24:188-92 pubmed publisher
  8. Hendriks I, Treffers L, Verlaan de Vries M, Olsen J, Vertegaal A. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Rep. 2015;: pubmed publisher
  9. Lu W, Liu S, Li B, Xie Y, Adhiambo C, Yang Q, et al. SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination. Oncotarget. 2015;6:771-88 pubmed
  10. Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Sinmyozu K, et al. Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem. 2014;289:28956-70 pubmed publisher
  11. Zou M, Cao J, Liu Z, Huh S, Polyak K, Yan Q. Histone demethylase jumonji AT-rich interactive domain 1B (JARID1B) controls mammary gland development by regulating key developmental and lineage specification genes. J Biol Chem. 2014;289:17620-33 pubmed publisher