This is a Validated Antibody Database (VAD) review about human JUNB, based on 16 published articles (read how Labome selects the articles), using JUNB antibody in all methods. It is aimed to help Labome visitors find the most suited JUNB antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
JUNB synonym: AP-1; transcription factor jun-B; activator protein 1; jun B proto-oncogene

Knockout validation
Cell Signaling Technology
rabbit monoclonal (C37F9)
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s2b
Cell Signaling Technology JUNB antibody (Cell Signaling, C37F9) was used in western blot knockout validation on mouse samples at 1:2000 (fig s2b). Nat Commun (2017) ncbi
Abcam
rabbit monoclonal (EPR6518)
  • immunohistochemistry; mouse; 1:250; fig 2d
In order to identify genes in endothelial cells activated following interactions with neurons during vascular development, Abcam JUNB antibody (Abcam, ab128878) was used in immunohistochemistry on mouse samples at 1:250 (fig 2d). J Cell Sci (2017) ncbi
rabbit monoclonal (EPR6518)
  • western blot; human; 1:1000; fig 3
Abcam JUNB antibody (Abcam, ab128878) was used in western blot on human samples at 1:1000 (fig 3). J Hematol Oncol (2015) ncbi
mouse monoclonal (204C4a)
  • EMSA; human
Abcam JUNB antibody (Abcam, ab53543) was used in EMSA on human samples . Int Immunol (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-11)
  • western blot; rat; 1:5000; loading ...; fig 2b
In order to elucidate the TrkB signaling-dependent expression of brain-derived neurotrophic factor, Santa Cruz Biotechnology JUNB antibody (Santa Cruz, sc-8051x) was used in western blot on rat samples at 1:5000 (fig 2b). J Neurosci (2016) ncbi
mouse monoclonal (C-11)
  • chromatin immunoprecipitation; human; fig 4
Santa Cruz Biotechnology JUNB antibody (Santa Cruz, SC-8051) was used in chromatin immunoprecipitation on human samples (fig 4). Kidney Int (2012) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse; loading ...; fig 8b
Proteintech Group JUNB antibody (ProteinTech, 10486-1-AP) was used in western blot on mouse samples (fig 8b). J Exp Med (2018) ncbi
Cell Signaling Technology
rabbit monoclonal (C37F9)
  • flow cytometry; mouse; loading ...; fig 5i
Cell Signaling Technology JUNB antibody (Cell Signaling, 3753) was used in flow cytometry on mouse samples (fig 5i). Nat Immunol (2018) ncbi
rabbit monoclonal (C37F9)
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s2b
Cell Signaling Technology JUNB antibody (Cell Signaling, C37F9) was used in western blot knockout validation on mouse samples at 1:2000 (fig s2b). Nat Commun (2017) ncbi
rabbit monoclonal (C37F9)
  • western blot; human; 1:1000; loading ...; fig 2c
In order to report FOSL1 as a common transcriptional signature across mutant KRAS cancers of distinct tissue origin, Cell Signaling Technology JUNB antibody (Cell Signaling, 3753) was used in western blot on human samples at 1:1000 (fig 2c). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3a
In order to identify genes in endothelial cells activated following interactions with neurons during vascular development, Cell Signaling Technology JUNB antibody (Cell Signaling, 3746) was used in western blot on mouse samples at 1:1000 (fig 3a). J Cell Sci (2017) ncbi
rabbit monoclonal (C37F9)
  • immunohistochemistry; human; 1:1000; loading ...; fig s5b
  • western blot; human; 1:1000; loading ...; fig s2a
In order to elucidate how CELF1 governs the epithelial-to-mesenchymal transition, Cell Signaling Technology JUNB antibody (Cell Signaling, 3753) was used in immunohistochemistry on human samples at 1:1000 (fig s5b) and in western blot on human samples at 1:1000 (fig s2a). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 7e
In order to propose that DLX1 modulates FOXM1 signaling to promote cancer aggressiveness via TGF-beta/SMAD4 signaling in high-grade serous ovarian cancer cells, Cell Signaling Technology JUNB antibody (Cell Signaling, 3746) was used in western blot on human samples (fig 7e). Oncogene (2017) ncbi
rabbit monoclonal (C37F9)
  • western blot; human; 1:1000; fig 5d
Cell Signaling Technology JUNB antibody (Cell Signaling, 3753) was used in western blot on human samples at 1:1000 (fig 5d). Nat Commun (2016) ncbi
rabbit monoclonal (C37F9)
  • immunohistochemistry - paraffin section; human; loading ...; fig s5b
In order to investigate metastatic melanoma through single-cell RNA-seq, Cell Signaling Technology JUNB antibody (Cell Signaling, C37F9) was used in immunohistochemistry - paraffin section on human samples (fig s5b). Science (2016) ncbi
rabbit monoclonal (C37F9)
  • chromatin immunoprecipitation; human; fig s9
  • immunoprecipitation; human; fig 5c
  • immunocytochemistry; human; fig 5d
  • western blot; human; fig 2c
Cell Signaling Technology JUNB antibody (Cell signalling technology, 3753) was used in chromatin immunoprecipitation on human samples (fig s9), in immunoprecipitation on human samples (fig 5c), in immunocytochemistry on human samples (fig 5d) and in western blot on human samples (fig 2c). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 2d
Cell Signaling Technology JUNB antibody (Cell Signaling, 3746) was used in western blot on human samples (fig 2d). Sci Rep (2014) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; 1:2000
EMD Millipore JUNB antibody (Merk-Millipore, 07-1333) was used in western blot on human samples at 1:2000. Placenta (2014) ncbi
Articles Reviewed
  1. Chen S, Yun F, Yao Y, Cao M, Zhang Y, Wang J, et al. USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein. J Exp Med. 2018;215:2850-2867 pubmed publisher
  2. Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley L, et al. Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol. 2018;19:594-605 pubmed publisher
  3. Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
  4. Vallejo A, Perurena N, Guruceaga E, Mazur P, Martínez Canarias S, Zandueta C, et al. An integrative approach unveils FOSL1 as an oncogene vulnerability in KRAS-driven lung and pancreatic cancer. Nat Commun. 2017;8:14294 pubmed publisher
  5. Yoshitomi Y, Ikeda T, Saito H, Yoshitake Y, Ishigaki Y, Hatta T, et al. JunB regulates angiogenesis and neurovascular parallel alignment in mouse embryonic skin. J Cell Sci. 2017;130:916-926 pubmed publisher
  6. Chaudhury A, Cheema S, Fachini J, Kongchan N, Lu G, Simon L, et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. Nat Commun. 2016;7:13362 pubmed publisher
  7. Chan D, Hui W, Wang J, Yung M, Hui L, Qin Y, et al. DLX1 acts as a crucial target of FOXM1 to promote ovarian cancer aggressiveness by enhancing TGF-β/SMAD4 signaling. Oncogene. 2017;36:1404-1416 pubmed publisher
  8. Xu Z, Bu Y, Chitnis N, Koumenis C, Fuchs S, Diehl J. miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosis. Nat Commun. 2016;7:11422 pubmed publisher
  9. Tirosh I, Izar B, Prakadan S, Wadsworth M, Treacy D, Trombetta J, et al. Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq. Science. 2016;352:189-96 pubmed publisher
  10. Chang H, Liu Y, Xue M, Liu H, Du S, Zhang L, et al. Synergistic action of master transcription factors controls epithelial-to-mesenchymal transition. Nucleic Acids Res. 2016;44:2514-27 pubmed publisher
  11. Tuvikene J, Pruunsild P, Orav E, Esvald E, Timmusk T. AP-1 Transcription Factors Mediate BDNF-Positive Feedback Loop in Cortical Neurons. J Neurosci. 2016;36:1290-305 pubmed publisher
  12. Suwei D, Liang Z, Zhimin L, Ruilei L, Yingying Z, Zhen L, et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol. 2015;8:120 pubmed publisher
  13. Shaiken T, Opekun A. Dissecting the cell to nucleus, perinucleus and cytosol. Sci Rep. 2014;4:4923 pubmed publisher
  14. Nuzzo A, Giuffrida D, Zenerino C, Piazzese A, Olearo E, Todros T, et al. JunB/cyclin-D1 imbalance in placental mesenchymal stromal cells derived from preeclamptic pregnancies with fetal-placental compromise. Placenta. 2014;35:483-90 pubmed publisher
  15. Davidson C, Cameron L, Burshtyn D. The AP-1 transcription factor JunD activates the leukocyte immunoglobulin-like receptor 1 distal promoter. Int Immunol. 2014;26:21-33 pubmed publisher
  16. Kim J, Zarjou A, Traylor A, Bolisetty S, Jaimes E, Hull T, et al. In vivo regulation of the heme oxygenase-1 gene in humanized transgenic mice. Kidney Int. 2012;82:278-91 pubmed publisher