This is a Validated Antibody Database (VAD) review about human ISG15, based on 13 published articles (read how Labome selects the articles), using ISG15 antibody in all methods. It is aimed to help Labome visitors find the most suited ISG15 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ISG15 synonym: G1P2; IFI15; IMD38; IP17; UCRP; hUCRP

Santa Cruz Biotechnology
mouse monoclonal (A-5)
  • western blot; mouse; fig 2b
Santa Cruz Biotechnology ISG15 antibody (santa, sc-166712) was used in western blot on mouse samples (fig 2b). J Immunol (2018) ncbi
mouse monoclonal (F-9)
  • western blot; human; 1:1000; loading ...; fig 4b
Santa Cruz Biotechnology ISG15 antibody (SantaCruz, F-9) was used in western blot on human samples at 1:1000 (fig 4b). J Virol (2018) ncbi
mouse monoclonal (3E5)
  • western blot; human; fig 1
Santa Cruz Biotechnology ISG15 antibody (Santa Cruz, sc-69,701) was used in western blot on human samples (fig 1). Neoplasia (2016) ncbi
mouse monoclonal (F-9)
  • immunocytochemistry; human; fig 1B
  • western blot; human; fig 1A
Santa Cruz Biotechnology ISG15 antibody (Santa Cruz, F-9) was used in immunocytochemistry on human samples (fig 1B) and in western blot on human samples (fig 1A). PLoS Pathog (2016) ncbi
mouse monoclonal (F-9)
  • western blot; rat; fig 3
Santa Cruz Biotechnology ISG15 antibody (santa Cruz, sc-166755) was used in western blot on rat samples (fig 3). PLoS ONE (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig 1
Abcam ISG15 antibody (Abcam, ab14374) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig 1). Acta Neuropathol Commun (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; fig 2
  • western blot; human; fig 4
Abcam ISG15 antibody (Abcam, ab131119) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 2) and in western blot on human samples (fig 4). Oncotarget (2016) ncbi
NKMAX
mouse monoclonal (3E5)
  • western blot; human
NKMAX ISG15 antibody (ATGen, AIS0701) was used in western blot on human samples . PLoS ONE (2014) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s6c
Cell Signaling Technology ISG15 antibody (Cell Signaling, 2743) was used in western blot on mouse samples at 1:1000 (fig s6c). Science (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2c
  • western blot; mouse; loading ...; fig 2c
In order to discover that PTEN is a substrate of the ISGylation post-translational modification pathway, Cell Signaling Technology ISG15 antibody (Cell Signaling, 2743) was used in western blot on human samples (fig 2c) and in western blot on mouse samples (fig 2c). Oncotarget (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 2
Cell Signaling Technology ISG15 antibody (Cell Signaling, 2743) was used in western blot on human samples (fig 2). elife (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
Cell Signaling Technology ISG15 antibody (Cell Signaling, 2743) was used in western blot on human samples (fig 4). PLoS Pathog (2016) ncbi
domestic rabbit monoclonal (22D2)
  • western blot; human; fig 1
Cell Signaling Technology ISG15 antibody (Cell Signaling Technology, #2758) was used in western blot on human samples (fig 1). Liver Int (2014) ncbi
Articles Reviewed
  1. Kim J, Gupta R, Blanco L, Yang S, Shteinfer Kuzmine A, Wang K, et al. VDAC oligomers form mitochondrial pores to release mtDNA fragments and promote lupus-like disease. Science. 2019;366:1531-1536 pubmed publisher
  2. Napolitano A, van der Veen A, Bunyan M, Borg A, Frith D, Howell S, et al. Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection. J Immunol. 2018;201:604-614 pubmed publisher
  3. Chiang C, Pauli E, Biryukov J, Feister K, Meng M, White E, et al. The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling. J Virol. 2018;92: pubmed publisher
  4. Mustachio L, Kawakami M, Lu Y, Rodriguez Canales J, Mino B, Behrens C, et al. The ISG15-specific protease USP18 regulates stability of PTEN. Oncotarget. 2017;8:3-14 pubmed publisher
  5. Park S, Yoon S, Kim H, Kim K. 90K Glycoprotein Promotes Degradation of Mutant ?-Catenin Lacking the ISGylation or Phosphorylation Sites in the N-terminus. Neoplasia. 2016;18:618-625 pubmed publisher
  6. Kim Y, Kim E, Kim Y, Lee M, Kwon K, Kim K, et al. Consecutive Inhibition of ISG15 Expression and ISGylation by Cytomegalovirus Regulators. PLoS Pathog. 2016;12:e1005850 pubmed publisher
  7. Sullivan K, Lewis H, Hill A, Pandey A, Jackson L, Cabral J, et al. Trisomy 21 consistently activates the interferon response. elife. 2016;5: pubmed publisher
  8. Preuße C, Allenbach Y, Hoffmann O, Goebel H, Pehl D, Radke J, et al. Differential roles of hypoxia and innate immunity in juvenile and adult dermatomyositis. Acta Neuropathol Commun. 2016;4:45 pubmed publisher
  9. Chen R, Du Y, Han P, Wang H, Liang F, Feng G, et al. ISG15 predicts poor prognosis and promotes cancer stem cell phenotype in nasopharyngeal carcinoma. Oncotarget. 2016;7:16910-22 pubmed publisher
  10. Suzuki Y, Chin W, Han Q, Ichiyama K, Lee C, Eyo Z, et al. Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication. PLoS Pathog. 2016;12:e1005357 pubmed publisher
  11. Gilda J, Ghosh R, Cheah J, West T, Bodine S, Gomes A. Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS ONE. 2015;10:e0135392 pubmed publisher
  12. Zahoor M, Xue G, Sato H, Murakami T, Takeshima S, Aida Y. HIV-1 Vpr induces interferon-stimulated genes in human monocyte-derived macrophages. PLoS ONE. 2014;9:e106418 pubmed publisher
  13. Hou Z, Han Q, Zhang C, Tian Z, Zhang J. miR146a impairs the IFN-induced anti-HBV immune response by downregulating STAT1 in hepatocytes. Liver Int. 2014;34:58-68 pubmed publisher