This is a Validated Antibody Database (VAD) review about human IRF8, based on 13 published articles (read how Labome selects the articles), using IRF8 antibody in all methods. It is aimed to help Labome visitors find the most suited IRF8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IRF8 synonym: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (E-9)
  • western blot knockout validation; human; loading ...; fig 3e
  • chromatin immunoprecipitation; human; loading ...; fig 3f
Santa Cruz Biotechnology IRF8 antibody (Santa Cruz, sc-365042) was used in western blot knockout validation on human samples (fig 3e) and in chromatin immunoprecipitation on human samples (fig 3f). Cancers (Basel) (2021) ncbi
Invitrogen
mouse monoclonal (GW4CML3)
  • western blot; mouse; fig 6c
Invitrogen IRF8 antibody (eBiosciences, 14-7888-82) was used in western blot on mouse samples (fig 6c). Cell (2021) ncbi
mouse monoclonal (V3GYWCH)
  • flow cytometry; mouse; loading ...; fig 4b
  • mass cytometry; human; loading ...; fig 1b, 1d, s2
  • flow cytometry; human; loading ...; fig 4b
Invitrogen IRF8 antibody (Thermo, V3GYWCH) was used in flow cytometry on mouse samples (fig 4b), in mass cytometry on human samples (fig 1b, 1d, s2) and in flow cytometry on human samples (fig 4b). Cell Rep (2019) ncbi
mouse monoclonal (V3GYWCH)
  • flow cytometry; mouse; loading ...; fig ex2c
Invitrogen IRF8 antibody (ThermoFisher, V3GYWCH) was used in flow cytometry on mouse samples (fig ex2c). Nat Med (2019) ncbi
mouse monoclonal (V3GYWCH)
  • flow cytometry; mouse; fig 1b
Invitrogen IRF8 antibody (eBiosciences, 17-9852-80) was used in flow cytometry on mouse samples (fig 1b). Cell (2018) ncbi
mouse monoclonal (V3GYWCH)
  • flow cytometry; human; loading ...; fig s14c
In order to map the lineage of human dendritic cells, Invitrogen IRF8 antibody (eBiosciences, V3GYWCH) was used in flow cytometry on human samples (fig s14c). Science (2017) ncbi
mouse monoclonal (V3GYWCH)
  • flow cytometry; human; loading ...; fig st12
In order to identify new types of human blood dendritic cells, monocytes, and progenitors through single-cell RNA-seq, Invitrogen IRF8 antibody (eBioscience, V3GYWCH) was used in flow cytometry on human samples (fig st12). Science (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-9)
  • western blot knockout validation; human; loading ...; fig 3e
  • chromatin immunoprecipitation; human; loading ...; fig 3f
Santa Cruz Biotechnology IRF8 antibody (Santa Cruz, sc-365042) was used in western blot knockout validation on human samples (fig 3e) and in chromatin immunoprecipitation on human samples (fig 3f). Cancers (Basel) (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D20D8)
  • western blot; mouse; loading ...; fig 5h
Cell Signaling Technology IRF8 antibody (Cell Signaling, 5628) was used in western blot on mouse samples (fig 5h). Front Immunol (2021) ncbi
domestic rabbit monoclonal (D20D8)
  • ChIP-Seq; human; 1-2 ug / 500 ul chr; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Cell Signaling Technology IRF8 antibody (Cell Signaling, 5628) was used in ChIP-Seq on human samples at 1-2 ug / 500 ul chr (fig 4c) and in chromatin immunoprecipitation on human samples (fig 4c). Cell Rep (2019) ncbi
domestic rabbit monoclonal (D20D8)
  • western blot; human; loading ...; fig 4a
Cell Signaling Technology IRF8 antibody (Cell Signaling, 5628S) was used in western blot on human samples (fig 4a). Front Immunol (2018) ncbi
domestic rabbit monoclonal (D20D8)
  • western blot; mouse; loading ...; fig 2d
Cell Signaling Technology IRF8 antibody (Cell Signaling, 5628) was used in western blot on mouse samples (fig 2d). Cell Death Dis (2016) ncbi
domestic rabbit monoclonal (D20D8)
  • western blot; mouse
In order to identify DUBA as a suppressor of IL-17 production in T cells, Cell Signaling Technology IRF8 antibody (Cell Signaling, D20D8) was used in western blot on mouse samples . Nature (2015) ncbi
domestic rabbit monoclonal (D20D8)
  • western blot; mouse
Cell Signaling Technology IRF8 antibody (Cell Signaling, 5628) was used in western blot on mouse samples . J Virol (2014) ncbi
Articles Reviewed
  1. Li B, Lian M, Li Y, Qian Q, Zhang J, Liu Q, et al. Myeloid-Derived Suppressive Cells Deficient in Liver X Receptor α Protected From Autoimmune Hepatitis. Front Immunol. 2021;12:732102 pubmed publisher
  2. Gangoso E, Southgate B, Bradley L, Rus S, Gálvez Cancino F, McGivern N, et al. Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion. Cell. 2021;184:2454-2470.e26 pubmed publisher
  3. Liss F, Frech M, Wang Y, Giel G, Fischer S, Simon C, et al. IRF8 Is an AML-Specific Susceptibility Factor That Regulates Signaling Pathways and Proliferation of AML Cells. Cancers (Basel). 2021;13: pubmed publisher
  4. Senigl F, Maman Y, Dinesh R, Alinikula J, Seth R, Pecnova L, et al. Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation. Cell Rep. 2019;29:3902-3915.e8 pubmed publisher
  5. Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  6. Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  7. Agod Z, Pazmandi K, Bencze D, Vereb G, Biro T, Szabo A, et al. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8. Front Immunol. 2018;9:62 pubmed publisher
  8. Böttcher J, Bonavita E, Chakravarty P, Blees H, Cabeza Cabrerizo M, Sammicheli S, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control. Cell. 2018;172:1022-1037.e14 pubmed publisher
  9. See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  10. Villani A, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017;356: pubmed publisher
  11. Fujiwara T, Zhou J, Ye S, Zhao H. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis. 2016;7:e2300 pubmed publisher
  12. Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher
  13. Schountz T, Quackenbush S, Rovnak J, Haddock E, Black W, Feldmann H, et al. Differential lymphocyte and antibody responses in deer mice infected with Sin Nombre hantavirus or Andes hantavirus. J Virol. 2014;88:8319-31 pubmed publisher