This is a Validated Antibody Database (VAD) review about human IRF5, based on 7 published articles (read how Labome selects the articles), using IRF5 antibody in all methods. It is aimed to help Labome visitors find the most suited IRF5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IRF5 synonym: SLEB10

Knockout validation
Abcam
domestic rabbit monoclonal (EPR17067)
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
  • flow cytometry; human; fig 3
  • western blot; human; fig 1
  • western blot knockout validation; mouse; fig 5
  • immunohistochemistry - paraffin section; mouse; fig 5
  • flow cytometry; mouse; fig 5
Abcam IRF5 antibody (Abcam, ab181553) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4), in flow cytometry on human samples (fig 3), in western blot on human samples (fig 1), in western blot knockout validation on mouse samples (fig 5), in immunohistochemistry - paraffin section on mouse samples (fig 5) and in flow cytometry on mouse samples (fig 5). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; fig 5
  • immunohistochemistry - paraffin section; mouse
  • flow cytometry; mouse
  • western blot; human
Cell Signaling Technology IRF5 antibody (Cell Signaling, 4950) was used in western blot knockout validation on mouse samples (fig 5), in immunohistochemistry - paraffin section on mouse samples , in flow cytometry on mouse samples and in western blot on human samples . Sci Rep (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR17067)
  • western blot; mouse; 1:1000; loading ...; fig s7g
Abcam IRF5 antibody (Abcam, ab181553) was used in western blot on mouse samples at 1:1000 (fig s7g). FASEB J (2022) ncbi
mouse monoclonal (10T1)
  • immunocytochemistry; human; loading ...; fig 3c
Abcam IRF5 antibody (Abcam, 10T1) was used in immunocytochemistry on human samples (fig 3c). Front Immunol (2017) ncbi
domestic rabbit monoclonal (EPR17067)
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
  • flow cytometry; human; fig 3
  • western blot; human; fig 1
  • western blot knockout validation; mouse; fig 5
  • immunohistochemistry - paraffin section; mouse; fig 5
  • flow cytometry; mouse; fig 5
Abcam IRF5 antibody (Abcam, ab181553) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4), in flow cytometry on human samples (fig 3), in western blot on human samples (fig 1), in western blot knockout validation on mouse samples (fig 5), in immunohistochemistry - paraffin section on mouse samples (fig 5) and in flow cytometry on mouse samples (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (10T1)
Abcam IRF5 antibody (Abcam, ab33478) was used . Sci Rep (2016) ncbi
mouse monoclonal (10T1)
  • immunocytochemistry; mouse; 1 ug/ml
  • western blot; mouse
Abcam IRF5 antibody (Abcam, ab33478) was used in immunocytochemistry on mouse samples at 1 ug/ml and in western blot on mouse samples . J Biol Chem (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (10T1)
  • western blot; human; loading ...; fig 7b
Santa Cruz Biotechnology IRF5 antibody (Santa Cruz Biotechnology, 10T1) was used in western blot on human samples (fig 7b). J Clin Invest (2016) ncbi
mouse monoclonal (10T1)
  • western blot; human; fig 6
Santa Cruz Biotechnology IRF5 antibody (Santa, sc-56714) was used in western blot on human samples (fig 6). Br J Haematol (2012) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (E1N9G)
  • flow cytometry; human; fig 3
  • western blot; human; fig 1
Cell Signaling Technology IRF5 antibody (Cell Signaling, 13496) was used in flow cytometry on human samples (fig 3) and in western blot on human samples (fig 1). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; fig 5
  • immunohistochemistry - paraffin section; mouse
  • flow cytometry; mouse
  • western blot; human
Cell Signaling Technology IRF5 antibody (Cell Signaling, 4950) was used in western blot knockout validation on mouse samples (fig 5), in immunohistochemistry - paraffin section on mouse samples , in flow cytometry on mouse samples and in western blot on human samples . Sci Rep (2016) ncbi
domestic rabbit monoclonal (E1N9G)
  • western blot; human; 1:1000; loading ...; fig 2c
Cell Signaling Technology IRF5 antibody (Cell Signaling, E1N9G) was used in western blot on human samples at 1:1000 (fig 2c). Int J Mol Med (2016) ncbi
Articles Reviewed
  1. Li D, Li J, Liu H, Zhai L, Hu W, Xia N, et al. Pathogenic Tconvs promote inflammatory macrophage polarization through GM-CSF and exacerbate abdominal aortic aneurysm formation. FASEB J. 2022;36:e22172 pubmed publisher
  2. Ehrnström B, Beckwith K, Yurchenko M, Moen S, Kojen J, Lentini G, et al. Toll-Like Receptor 8 Is a Major Sensor of Group B Streptococcus But Not Escherichia coli in Human Primary Monocytes and Macrophages. Front Immunol. 2017;8:1243 pubmed publisher
  3. Achuthan A, Cook A, Lee M, Saleh R, Khiew H, Chang M, et al. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation. J Clin Invest. 2016;126:3453-66 pubmed publisher
  4. De S, Li D, Song S, Matta B, Barnes B. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep. 2016;6:31002 pubmed publisher
  5. Xie C, Liu C, Wu B, Lin Y, Ma T, Xiong H, et al. Effects of IRF1 and IFN-? interaction on the M1 polarization of macrophages and its antitumor function. Int J Mol Med. 2016;38:148-60 pubmed publisher
  6. He H, Zhang S, Tighe S, Son J, Tseng S. Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype. J Biol Chem. 2013;288:25792-803 pubmed publisher
  7. Fresquet V, Robles E, Parker A, Martinez Useros J, Mena M, Malumbres R, et al. High-throughput sequencing analysis of the chromosome 7q32 deletion reveals IRF5 as a potential tumour suppressor in splenic marginal-zone lymphoma. Br J Haematol. 2012;158:712-26 pubmed publisher