This is a Validated Antibody Database (VAD) review about human IRAK4, based on 12 published articles (read how Labome selects the articles), using IRAK4 antibody in all methods. It is aimed to help Labome visitors find the most suited IRAK4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IRAK4 synonym: IPD1; IRAK-4; NY-REN-64; REN64; interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64

Knockout validation
Invitrogen
mouse monoclonal (2H9)
  • western blot knockout validation; mouse; fig 5
  • western blot knockout validation; human; fig 5
In order to identify species-specific preferences in signaling protein use by comprehensive RNAi-based screening of human and mouse TLR pathways, Invitrogen IRAK4 antibody (Pierce, MA5-15883) was used in western blot knockout validation on mouse samples (fig 5) and in western blot knockout validation on human samples (fig 5). Sci Signal (2016) ncbi
Abnova
mouse monoclonal (2H9)
  • western blot; human; 1:500; loading ...; fig 2g
Abnova IRAK4 antibody (Abnova, MAB10701) was used in western blot on human samples at 1:500 (fig 2g). Biochem Biophys Res Commun (2018) ncbi
mouse monoclonal (A8A8)
  • western blot; human; 1:500; loading ...; fig 2g
Abnova IRAK4 antibody (Abnova, MAB2538) was used in western blot on human samples at 1:500 (fig 2g). Biochem Biophys Res Commun (2018) ncbi
Abcam
rabbit monoclonal (Y279)
  • immunohistochemistry; human; 1:200; tbl 2
In order to evaluate the reliance on EBV or NF-kappaB signaling instead of B-cell receptor signaling in regards to complete phenotypic characterization of PTLD, Abcam IRAK4 antibody (Abcam, ab32511) was used in immunohistochemistry on human samples at 1:200 (tbl 2). Hematol Oncol (2017) ncbi
rabbit monoclonal (Y279)
  • western blot; mouse; fig 4
  • western blot; human; fig 4
In order to identify species-specific preferences in signaling protein use by comprehensive RNAi-based screening of human and mouse TLR pathways, Abcam IRAK4 antibody (Abcam, ab32511) was used in western blot on mouse samples (fig 4) and in western blot on human samples (fig 4). Sci Signal (2016) ncbi
Invitrogen
mouse monoclonal (2H9)
  • western blot knockout validation; mouse; fig 5
  • western blot knockout validation; human; fig 5
In order to identify species-specific preferences in signaling protein use by comprehensive RNAi-based screening of human and mouse TLR pathways, Invitrogen IRAK4 antibody (Pierce, MA5-15883) was used in western blot knockout validation on mouse samples (fig 5) and in western blot knockout validation on human samples (fig 5). Sci Signal (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D6D7)
  • western blot; mouse; fig 6b
Cell Signaling Technology IRAK4 antibody (Cell Signaling, 11927) was used in western blot on mouse samples (fig 6b). Infect Immun (2017) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6b
Cell Signaling Technology IRAK4 antibody (Cell Signaling, 4363) was used in western blot on mouse samples (fig 6b). Infect Immun (2017) ncbi
rabbit monoclonal (D6D7)
  • western blot; human; loading ...; fig 4a
In order to study the the activation of T lymphocyte death by Ebola virus glycoprotein, Cell Signaling Technology IRAK4 antibody (Cell Signaling, 11927S) was used in western blot on human samples (fig 4a). PLoS Pathog (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4a
In order to study the the activation of T lymphocyte death by Ebola virus glycoprotein, Cell Signaling Technology IRAK4 antibody (Cell Signaling, 4363S) was used in western blot on human samples (fig 4a). PLoS Pathog (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 3c
Cell Signaling Technology IRAK4 antibody (Cell Signaling, 4363) was used in western blot on human samples (fig 3c). Oncotarget (2017) ncbi
rabbit polyclonal
Cell Signaling Technology IRAK4 antibody (Cell Signaling, 4363) was used . Sci Rep (2016) ncbi
rabbit monoclonal (D6D7)
  • western blot; human; 1:100; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology IRAK4 antibody (Cell Signaling, 11927) was used in western blot on human samples at 1:100 (fig st1). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology IRAK4 antibody (Cell Signaling, 4363P) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 5d
In order to study the contribution of neutrophil extracellular traps to pulmonary hypertension, Cell Signaling Technology IRAK4 antibody (Cell Signaling, 4363P) was used in western blot on human samples (fig 5d). Arterioscler Thromb Vasc Biol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; fig 3
Cell Signaling Technology IRAK4 antibody (Cell Signaling Tech, 4363) was used in western blot on mouse samples at 1:500 (fig 3). J Am Heart Assoc (2016) ncbi
rabbit polyclonal
  • western blot; mouse
Cell Signaling Technology IRAK4 antibody (Cell Signaling Technology, 4363) was used in western blot on mouse samples . J Immunol (2012) ncbi
ProSci
rabbit polyclonal
  • western blot; human
In order to study the role of Unc5CL in the pro-inflammatory IRAK signaling cascade, ProSci IRAK4 antibody (ProSci, 3125) was used in western blot on human samples . Cell Death Differ (2012) ncbi
Articles Reviewed
  1. Hiromura M, Nohtomi K, Mori Y, Kataoka H, Sugano M, Ohnuma K, et al. Caveolin-1, a binding protein of CD26, is essential for the anti-inflammatory effects of dipeptidyl peptidase-4 inhibitors on human and mouse macrophages. Biochem Biophys Res Commun. 2018;495:223-229 pubmed publisher
  2. Naik S, Padhi A, Ganguli G, Sengupta S, Pati S, Das D, et al. Mouse Bone Marrow Sca-1+ CD44+ Mesenchymal Stem Cells Kill Avirulent Mycobacteria but Not Mycobacterium tuberculosis through Modulation of Cathelicidin Expression via the p38 Mitogen-Activated Protein Kinase-Dependent Pathway. Infect Immun. 2017;85: pubmed publisher
  3. Iampietro M, Younan P, Nishida A, Dutta M, Lubaki N, Santos R, et al. Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathog. 2017;13:e1006397 pubmed publisher
  4. Mondello P, Derenzini E, Asgari Z, Philip J, Brea E, SESHAN V, et al. Dual inhibition of histone deacetylases and phosphoinositide 3-kinase enhances therapeutic activity against B cell lymphoma. Oncotarget. 2017;8:14017-14028 pubmed publisher
  5. Boro M, Singh V, Balaji K. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses. Sci Rep. 2016;6:37695 pubmed publisher
  6. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  7. Aldabbous L, Abdul Salam V, McKinnon T, Duluc L, Pepke Zaba J, Southwood M, et al. Neutrophil Extracellular Traps Promote Angiogenesis: Evidence From Vascular Pathology in Pulmonary Hypertension. Arterioscler Thromb Vasc Biol. 2016;36:2078-87 pubmed publisher
  8. Nishikido T, Oyama J, Shiraki A, Komoda H, Node K. Deletion of Apoptosis Inhibitor of Macrophage (AIM)/CD5L Attenuates the Inflammatory Response and Infarct Size in Acute Myocardial Infarction. J Am Heart Assoc. 2016;5:e002863 pubmed publisher
  9. Menter T, Dickenmann M, Juskevicius D, Steiger J, Dirnhofer S, Tzankov A. Comprehensive phenotypic characterization of PTLD reveals potential reliance on EBV or NF-κB signalling instead of B-cell receptor signalling. Hematol Oncol. 2017;35:187-197 pubmed publisher
  10. Sun J, Li N, Oh K, Dutta B, Vayttaden S, Lin B, et al. Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use. Sci Signal. 2016;9:ra3 pubmed publisher
  11. Heinz L, Rebsamen M, Rossi D, Staehli F, Schroder K, Quadroni M, et al. The death domain-containing protein Unc5CL is a novel MyD88-independent activator of the pro-inflammatory IRAK signaling cascade. Cell Death Differ. 2012;19:722-31 pubmed publisher
  12. Xu L, Jin L, Zhang B, Akerlund L, Shu H, Cambier J. VISA is required for B cell expression of TLR7. J Immunol. 2012;188:248-58 pubmed publisher