This is a Validated Antibody Database (VAD) review about human IRAK, based on 22 published articles (read how Labome selects the articles), using IRAK antibody in all methods. It is aimed to help Labome visitors find the most suited IRAK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IRAK synonym: IRAK; pelle; interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog

Santa Cruz Biotechnology
mouse monoclonal (F-4)
  • western blot; mouse; 1:200; loading ...; fig 7d
Santa Cruz Biotechnology IRAK antibody (Santa Cruz, sc-5288) was used in western blot on mouse samples at 1:200 (fig 7d). F1000Res (2017) ncbi
mouse monoclonal (F-4)
  • western blot; human; 1:500; fig 4c
Santa Cruz Biotechnology IRAK antibody (SantaCruz, 5288) was used in western blot on human samples at 1:500 (fig 4c). Nat Cell Biol (2015) ncbi
GeneTex
rabbit polyclonal
  • western blot; human; 1:1000
GeneTex IRAK antibody (GeneTex, GTX111421) was used in western blot on human samples at 1:1000. Endocrinology (2015) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s7s
Proteintech Group IRAK antibody (Proteintech, 10478-2-AP) was used in western blot on mouse samples at 1:1000 (fig s7s). Nat Commun (2018) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:200; fig 1
  • immunocytochemistry; mouse; 1:200; fig 3
Abcam IRAK antibody (Abcam, ab238) was used in immunohistochemistry - free floating section on mouse samples at 1:200 (fig 1) and in immunocytochemistry on mouse samples at 1:200 (fig 3). Nat Commun (2016) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; fig 5c
In order to study the effect of human cytomegalovirus microRNAs on TLR2, Invitrogen IRAK antibody (Pierce, PA5-17490) was used in western blot on human samples (fig 5c). PLoS Pathog (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D51G7)
  • western blot; mouse; 1:1000; loading ...; fig 3a
Cell Signaling Technology IRAK antibody (Cell Signaling Technology, 4504) was used in western blot on mouse samples at 1:1000 (fig 3a). J Clin Invest (2018) ncbi
rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 3d
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504S) was used in western blot on human samples (fig 3d). J Virol (2018) ncbi
rabbit monoclonal (D51G7)
  • western blot; mouse; loading ...; fig 8a
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504S) was used in western blot on mouse samples (fig 8a). J Clin Invest (2017) ncbi
rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 6a
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used in western blot on human samples (fig 6a). J Virol (2017) ncbi
rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig s1d
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used in western blot on human samples (fig s1d). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (D51G7)
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used . Sci Rep (2016) ncbi
rabbit monoclonal (D51G7)
  • western blot; mouse; loading ...; fig 2h
In order to investigate how PI3Kgamma regulates macrophage polarization, Cell Signaling Technology IRAK antibody (Cell Signaling, D51G7) was used in western blot on mouse samples (fig 2h). Nature (2016) ncbi
rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 6c
Cell Signaling Technology IRAK antibody (Cell Signaling Technology, D51G7) was used in western blot on human samples (fig 6c). Cell Rep (2016) ncbi
rabbit monoclonal (D51G7)
  • western blot; human
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used in western blot on human samples . Biochem Biophys Res Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 6
Cell Signaling Technology IRAK antibody (Cell Signaling, 4359S) was used in western blot on human samples (fig 6). PLoS Pathog (2016) ncbi
rabbit monoclonal (D51G7)
  • western blot; human; fig 7
  • western blot; mouse; fig 8
In order to identify species-specific preferences in signaling protein use by comprehensive RNAi-based screening of human and mouse TLR pathways, Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used in western blot on human samples (fig 7) and in western blot on mouse samples (fig 8). Sci Signal (2016) ncbi
rabbit monoclonal (D51G7)
  • western blot; mouse; 1:500; fig 3
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used in western blot on mouse samples at 1:500 (fig 3). Nat Commun (2015) ncbi
rabbit monoclonal (D51G7)
  • western blot; human
Cell Signaling Technology IRAK antibody (Cell Signaling Technology, 4504) was used in western blot on human samples . Mol Biol Cell (2015) ncbi
rabbit monoclonal (D51G7)
  • flow cytometry; human; loading ...; fig 4b
Cell Signaling Technology IRAK antibody (Cell Signaling, 4504) was used in flow cytometry on human samples (fig 4b). Invest New Drugs (2015) ncbi
rabbit monoclonal (D51G7)
  • western blot; human; 1:10000
In order to identify an epigenetic mechanism of crosstalk between STAT3 and NF-kappaB that regulates constitutive STAT3 activation in oncogenesis, Cell Signaling Technology IRAK antibody (Cell Signaling Technology, 4504) was used in western blot on human samples at 1:10000. Sci Signal (2014) ncbi
rabbit monoclonal (D51G7)
  • western blot; mouse; fig 6c
Cell Signaling Technology IRAK antibody (Cell Signaling Technology, 4504) was used in western blot on mouse samples (fig 6c). J Immunol (2012) ncbi
Sigma-Aldrich
rabbit polyclonal
Sigma-Aldrich IRAK antibody (Sigma-Aldrich, SAB4504246) was used . Sci Rep (2016) ncbi
Articles Reviewed
  1. Zhang C, Jiang M, Zhou H, Liu W, Wang C, Kang Z, et al. TLR-stimulated IRAKM activates caspase-8 inflammasome in microglia and promotes neuroinflammation. J Clin Invest. 2018;128:5399-5412 pubmed publisher
  2. Xu W, Ma C, Zhang Q, Zhao R, Hu D, Zhang X, et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol. 2018;92: pubmed publisher
  3. Liu Z, Qin Q, Wu C, Li H, Shou J, Yang Y, et al. Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. Nat Commun. 2018;9:2789 pubmed publisher
  4. McCarthy G, Bridges C, Blednov Y, Harris R. CNS cell-type localization and LPS response of TLR signaling pathways. F1000Res. 2017;6:1144 pubmed publisher
  5. Li B, Wang X, Choi I, Wang Y, Liu S, Pham A, et al. miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity. J Clin Invest. 2017;127:3702-3716 pubmed publisher
  6. Franz S, Rennert P, Woznik M, Grützke J, Lüdde A, Arriero Pais E, et al. Mumps Virus SH Protein Inhibits NF-κB Activation by Interacting with Tumor Necrosis Factor Receptor 1, Interleukin-1 Receptor 1, and Toll-Like Receptor 3 Complexes. J Virol. 2017;91: pubmed publisher
  7. Strickson S, Emmerich C, Goh E, Zhang J, Kelsall I, MacArtney T, et al. Roles of the TRAF6 and Pellino E3 ligases in MyD88 and RANKL signaling. Proc Natl Acad Sci U S A. 2017;114:E3481-E3489 pubmed publisher
  8. Boro M, Singh V, Balaji K. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses. Sci Rep. 2016;6:37695 pubmed publisher
  9. Kaneda M, Messer K, Ralainirina N, Li H, Leem C, Gorjestani S, et al. PI3K? is a molecular switch that controls immune suppression. Nature. 2016;539:437-442 pubmed publisher
  10. Hedl M, Yan J, Abraham C. IRF5 and IRF5 Disease-Risk Variants Increase Glycolysis and Human M1 Macrophage Polarization by Regulating Proximal Signaling and Akt2 Activation. Cell Rep. 2016;16:2442-55 pubmed publisher
  11. Emmerich C, Bakshi S, Kelsall I, Ortiz Guerrero J, Shpiro N, Cohen P. Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling. Biochem Biophys Res Commun. 2016;474:452-461 pubmed publisher
  12. Jung A, Stoiber C, Herkt C, Schulz C, Bertrams W, Schmeck B. Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages. PLoS Pathog. 2016;12:e1005592 pubmed publisher
  13. Kishi N, MacDonald J, Ye J, Molyneaux B, Azim E, Macklis J. Reduction of aberrant NF-κB signalling ameliorates Rett syndrome phenotypes in Mecp2-null mice. Nat Commun. 2016;7:10520 pubmed publisher
  14. Sun J, Li N, Oh K, Dutta B, Vayttaden S, Lin B, et al. Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use. Sci Signal. 2016;9:ra3 pubmed publisher
  15. Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
  16. Alexander M, Hu R, Runtsch M, Kagele D, Mosbruger T, Tolmachova T, et al. Exosome-delivered microRNAs modulate the inflammatory response to endotoxin. Nat Commun. 2015;6:7321 pubmed publisher
  17. Zhong X, Liao Y, Chen L, Liu G, Feng Y, Zeng T, et al. The MicroRNAs in the Pathogenesis of Metabolic Memory. Endocrinology. 2015;156:3157-68 pubmed publisher
  18. Landais I, Pelton C, Streblow D, DeFilippis V, McWeeney S, Nelson J. Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway. PLoS Pathog. 2015;11:e1004881 pubmed publisher
  19. Willy J, Young S, Stevens J, Masuoka H, Wek R. CHOP links endoplasmic reticulum stress to NF-κB activation in the pathogenesis of nonalcoholic steatohepatitis. Mol Biol Cell. 2015;26:2190-204 pubmed publisher
  20. Guzmán E, Maers K, Roberts J, Kemami Wangun H, Harmody D, Wright A. The marine natural product microsclerodermin A is a novel inhibitor of the nuclear factor kappa B and induces apoptosis in pancreatic cancer cells. Invest New Drugs. 2015;33:86-94 pubmed publisher
  21. Xiang M, Birkbak N, Vafaizadeh V, Walker S, Yeh J, Liu S, et al. STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-?B to IL-6 signaling axis and STAT3-driven cancer phenotypes. Sci Signal. 2014;7:ra11 pubmed publisher
  22. Xu L, Jin L, Zhang B, Akerlund L, Shu H, Cambier J. VISA is required for B cell expression of TLR7. J Immunol. 2012;188:248-58 pubmed publisher