This is a Validated Antibody Database (VAD) review about human IQGAP1, based on 19 published articles (read how Labome selects the articles), using IQGAP1 antibody in all methods. It is aimed to help Labome visitors find the most suited IQGAP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IQGAP1 synonym: HUMORFA01; SAR1; p195

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5220)
  • western blot knockout validation; human; 1:1000; fig 3a
Abcam IQGAP1 antibody (Abcam, ab133490) was used in western blot knockout validation on human samples at 1:1000 (fig 3a). Sci Rep (2020) ncbi
Abcam
domestic rabbit monoclonal (EPR5220)
  • western blot knockout validation; human; 1:1000; fig 3a
Abcam IQGAP1 antibody (Abcam, ab133490) was used in western blot knockout validation on human samples at 1:1000 (fig 3a). Sci Rep (2020) ncbi
domestic rabbit monoclonal (EPR5220)
  • immunohistochemistry; human; loading ...; fig 5d
  • western blot; human; loading ...; fig 1a
Abcam IQGAP1 antibody (Abcam, ab133490) was used in immunohistochemistry on human samples (fig 5d) and in western blot on human samples (fig 1a). Cancer Lett (2017) ncbi
domestic rabbit monoclonal (EPR5220)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 5a
  • western blot; human; 1:1000; loading ...; fig 2a
Abcam IQGAP1 antibody (Abcam, ab133490) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 5a) and in western blot on human samples at 1:1000 (fig 2a). Oncol Rep (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-7)
  • immunocytochemistry; human; fig s1
Santa Cruz Biotechnology IQGAP1 antibody (Santa Cruz Biotechnology, sc-376383) was used in immunocytochemistry on human samples (fig s1). J Cell Sci (2016) ncbi
mouse monoclonal (C-9)
  • immunohistochemistry - paraffin section; human; 1:40
Santa Cruz Biotechnology IQGAP1 antibody (Santa Cruz Biotechnology, sc-376021) was used in immunohistochemistry - paraffin section on human samples at 1:40. PLoS ONE (2014) ncbi
mouse monoclonal (D-3)
  • western blot; human; loading ...; fig 3d
In order to perform a proteomic analysis of HBV-infected HepG2 cells to identify changes in proteins involved in cell migration, Santa Cruz Biotechnology IQGAP1 antibody (Santa Cruz, sc-374307) was used in western blot on human samples (fig 3d). PLoS ONE (2014) ncbi
Invitrogen
mouse monoclonal (AF1)
  • western blot; mouse; 1:3000; loading ...; fig 1j
In order to examine the role of AKAP220 in actin dynamics and AQP2 trafficking, Invitrogen IQGAP1 antibody (Invitrogen, 33-8900) was used in western blot on mouse samples at 1:3000 (fig 1j). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (AF1)
  • western blot; human; fig 1
In order to develop and use a high-throughput, in vivo strategy to determine which alterations are loss of function in tumor growth suppressors using bladder cancer samples, Invitrogen IQGAP1 antibody (Life Technologies, 33-8900) was used in western blot on human samples (fig 1). Mol Cancer Res (2015) ncbi
Proteintech Group
domestic rabbit polyclonal
Proteintech Group IQGAP1 antibody (ProteinTech, 22167-1-AP) was used . Oncotarget (2015) ncbi
BD Biosciences
mouse monoclonal (24/IQGAP1)
  • western blot; human; 1:1000; loading ...; fig s3b
BD Biosciences IQGAP1 antibody (BD Biosciences, 610611) was used in western blot on human samples at 1:1000 (fig s3b). Nat Commun (2020) ncbi
mouse monoclonal (24/IQGAP1)
  • immunoprecipitation; human; fig 5
  • western blot; human; fig 5
BD Biosciences IQGAP1 antibody (BD Transduction Laboratories, 610611) was used in immunoprecipitation on human samples (fig 5) and in western blot on human samples (fig 5). Mol Ther Methods Clin Dev (2016) ncbi
mouse monoclonal (24/IQGAP1)
  • western blot; mouse; 1:1000; fig 4
BD Biosciences IQGAP1 antibody (BD Biosciences, 610611) was used in western blot on mouse samples at 1:1000 (fig 4). Cell Cycle (2016) ncbi
mouse monoclonal (24/IQGAP1)
  • immunocytochemistry; mouse; 1:500; fig 5
In order to report a role for adenomatous polyposis coli in the transport of mitochondria, BD Biosciences IQGAP1 antibody (BD, 610612) was used in immunocytochemistry on mouse samples at 1:500 (fig 5). Mol Biol Cell (2016) ncbi
mouse monoclonal (24/IQGAP1)
  • western blot; human; 1:1000; fig 1
BD Biosciences IQGAP1 antibody (BD Biosciences, 610611) was used in western blot on human samples at 1:1000 (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (24/IQGAP1)
  • immunohistochemistry; human; fig 5
  • western blot; human; fig 3
In order to investigate the roles and mechanisms of the RSPO-LGR system in oncogenesis, BD Biosciences IQGAP1 antibody (BD Transduction Laboratories, 610611) was used in immunohistochemistry on human samples (fig 5) and in western blot on human samples (fig 3). Oncogene (2015) ncbi
mouse monoclonal (24/IQGAP1)
  • immunohistochemistry - paraffin section; human
BD Biosciences IQGAP1 antibody (Becton Dickinson, 610611) was used in immunohistochemistry - paraffin section on human samples . Cancer Lett (2015) ncbi
EMD Millipore
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:250; loading ...; fig 2c
EMD Millipore IQGAP1 antibody (Millipore, ABT186) was used in immunohistochemistry - paraffin section on human samples at 1:250 (fig 2c). J Histochem Cytochem (2019) ncbi
mouse monoclonal (AF4)
  • western blot; human; loading ...; fig 4a
EMD Millipore IQGAP1 antibody (EMD Millipore, 05-504) was used in western blot on human samples (fig 4a). Onco Targets Ther (2017) ncbi
mouse monoclonal (AF4)
  • immunocytochemistry; human; 1:100; loading ...; fig 3g
  • western blot; human; loading ...; fig 3b
EMD Millipore IQGAP1 antibody (Millipore, 05-504) was used in immunocytochemistry on human samples at 1:100 (fig 3g) and in western blot on human samples (fig 3b). Oncotarget (2016) ncbi
mouse monoclonal (AF4)
  • immunohistochemistry - paraffin section; human
EMD Millipore IQGAP1 antibody (Upstate/EMD Millipore, 05-504) was used in immunohistochemistry - paraffin section on human samples . Cancer Lett (2015) ncbi
Articles Reviewed
  1. Hebert J, Tian C, Lamar J, Rickelt S, Abbruzzese G, Liu X, et al. The scaffold protein IQGAP1 is crucial for extravasation and metastasis. Sci Rep. 2020;10:2439 pubmed publisher
  2. Kennedy S, Jarboui M, Srihari S, Raso C, Bryan K, Dernayka L, et al. Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRASG13D. Nat Commun. 2020;11:499 pubmed publisher
  3. Rotoli D, Morales M, Maeso M, Avila J, Pérez Rodríguez N, Mobasheri A, et al. IQGAP1, AmotL2, and FKBP51 Scaffoldins in the Glioblastoma Microenvironment. J Histochem Cytochem. 2019;67:481-494 pubmed publisher
  4. Liang Z, Yang Y, He Y, Yang P, Wang X, He G, et al. SUMOylation of IQGAP1 promotes the development of colorectal cancer. Cancer Lett. 2017;411:90-99 pubmed publisher
  5. Cui Y, Li Q, Li H, Wang Y, Wang H, Chen W, et al. Asparaginyl endopeptidase improves the resistance of microtubule-targeting drugs in gastric cancer through IQGAP1 modulating the EGFR/JNK/ERK signaling pathway. Onco Targets Ther. 2017;10:627-643 pubmed publisher
  6. Cui X, Song L, Bai Y, Wang Y, Wang B, Wang W. Elevated IQGAP1 and CDC42 levels correlate with tumor malignancy of human glioma. Oncol Rep. 2017;37:768-776 pubmed publisher
  7. Wang H, Han X, Bretz C, Becker S, Gambhir D, Smith G, et al. Retinal pigment epithelial cell expression of active Rap 1a by scAAV2 inhibits choroidal neovascularization. Mol Ther Methods Clin Dev. 2016;3:16056 pubmed publisher
  8. Lagares Tena L, García Monclús S, López Alemany R, Almacellas Rabaiget O, Huertas Martínez J, Sáinz Jaspeado M, et al. Caveolin-1 promotes Ewing sarcoma metastasis regulating MMP-9 expression through MAPK/ERK pathway. Oncotarget. 2016;7:56889-56903 pubmed publisher
  9. Whiting J, Ogier L, Forbush K, Bucko P, Gopalan J, Seternes O, et al. AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption. Proc Natl Acad Sci U S A. 2016;113:E4328-37 pubmed publisher
  10. Daubon T, Spuul P, Alonso F, Fremaux I, Genot E. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. J Cell Sci. 2016;129:2586-98 pubmed publisher
  11. Marei H, Carpy A, Macek B, Malliri A. Proteomic analysis of Rac1 signaling regulation by guanine nucleotide exchange factors. Cell Cycle. 2016;15:1961-74 pubmed publisher
  12. Mills K, Brocardo M, Henderson B. APC binds the Miro/Milton motor complex to stimulate transport of mitochondria to the plasma membrane. Mol Biol Cell. 2016;27:466-82 pubmed publisher
  13. Hensel J, Duex J, Owens C, Dancik G, Edwards M, Frierson H, et al. Patient Mutation Directed shRNA Screen Uncovers Novel Bladder Tumor Growth Suppressors. Mol Cancer Res. 2015;13:1306-15 pubmed publisher
  14. Moon H, Ruelcke J, Choi E, Sharpe L, Nassar Z, Bielefeldt Ohmann H, et al. Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1. Oncotarget. 2015;6:7438-53 pubmed
  15. Morris E, Assi K, Salh B, Dedhar S. Integrin-linked kinase links dynactin-1/dynactin-2 with cortical integrin receptors to orient the mitotic spindle relative to the substratum. Sci Rep. 2015;5:8389 pubmed publisher
  16. Gong X, Yi J, Carmon K, Crumbley C, Xiong W, Thomas A, et al. Aberrant RSPO3-LGR4 signaling in Keap1-deficient lung adenocarcinomas promotes tumor aggressiveness. Oncogene. 2015;34:4692-701 pubmed publisher
  17. Zhao H, Xie C, Lin X, Zhao Y, Han Y, Fan C, et al. Coexpression of IQ-domain GTPase-activating protein 1 (IQGAP1) and Dishevelled (Dvl) is correlated with poor prognosis in non-small cell lung cancer. PLoS ONE. 2014;9:e113713 pubmed publisher
  18. Holck S, Nielsen H, Hammer E, Christensen I, Larsson L. IQGAP1 in rectal adenocarcinomas: localization and protein expression before and after radiochemotherapy. Cancer Lett. 2015;356:556-60 pubmed publisher
  19. Feng H, Li X, Chan V, Chen W. Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells. PLoS ONE. 2014;9:e95621 pubmed publisher