This is a Validated Antibody Database (VAD) review about human INCENP, based on 11 published articles (read how Labome selects the articles), using INCENP antibody in all methods. It is aimed to help Labome visitors find the most suited INCENP antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
INCENP synonym: inner centromere protein; binds and activates aurora-B and -C in vivo and in vitro; chromosomal passenger protein; inner centromere protein INCENP; inner centromere protein antigens 135/155kDa

Abcam
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 6a
  • western blot; human; loading ...; fig 8
In order to identify inhibitors of the Aurora B::INCENP interaction, Abcam INCENP antibody (Abcam, 12183) was used in immunocytochemistry on human samples (fig 6a) and in western blot on human samples (fig 8). Chem Biol Drug Des (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4b
In order to study the capacity of Fatostatin to inhibit sterol regulatory element-binding protein activity and cell division, Abcam INCENP antibody (Abcam, ab12183) was used in immunocytochemistry on human samples (fig 4b). J Biol Chem (2016) ncbi
mouse monoclonal (58-217)
  • immunocytochemistry; human; fig 4
Abcam INCENP antibody (Abcam, ab23956) was used in immunocytochemistry on human samples (fig 4). elife (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; red rock crab; 1:200
Abcam INCENP antibody (Abcam, ab12183) was used in immunohistochemistry on red rock crab samples at 1:200. PLoS ONE (2012) ncbi
Invitrogen
mouse monoclonal (58-217)
  • immunocytochemistry; human; 1:150; loading ...; fig 3
In order to analyze the localization of chromosomal passenger complex (CPC) after treatment with HDACi during cell division., Invitrogen INCENP antibody (Life Techonlogies, 39-2800) was used in immunocytochemistry on human samples at 1:150 (fig 3). Methods Mol Biol (2017) ncbi
mouse monoclonal (58-217)
  • immunocytochemistry; human
In order to study the colocalization of Ser2481-autophosphorylated mTOR and chromosomal passenger proteins during mammalian cell cytokinesis, Invitrogen INCENP antibody (Zymed Laboratories, 39-2800) was used in immunocytochemistry on human samples . Cell Cycle (2012) ncbi
mouse monoclonal (58-217)
  • western blot; human; 1:500; fig 4h
In order to report the dimethylation of HSP70 at Lys-561 by SETD1A, Invitrogen INCENP antibody (Invitrogen, 39-2800) was used in western blot on human samples at 1:500 (fig 4h). Nat Commun (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-4)
  • immunohistochemistry; human; 1:150; loading ...; fig s4a
In order to describe a novel role for the neuronal apoptosis inhibitory protein in addition to anti-apoptotic and innate immunology functions, Santa Cruz Biotechnology INCENP antibody (Santa Cruz, sc-376514) was used in immunohistochemistry on human samples at 1:150 (fig s4a). Sci Rep (2017) ncbi
Active Motif
mouse monoclonal
  • immunocytochemistry; human; 1:1,000
Active Motif INCENP antibody (Active Motif, 39259) was used in immunocytochemistry on human samples at 1:1,000. J Cell Biol (2011) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 3
Cell Signaling Technology INCENP antibody (Cell Signaling, 2807) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 3). Open Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Cell Signaling Technology INCENP antibody (Cell Signaling, 2786) was used in western blot on human samples (fig 5). Cell Mol Life Sci (2016) ncbi
Articles Reviewed
  1. Abadía Molina F, Morón Calvente V, Baird S, Shamim F, Martin F, MacKenzie A. Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division. Sci Rep. 2017;7:39981 pubmed publisher
  2. Unruhe Knauf B, Knauer S. Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization. Methods Mol Biol. 2017;1510:47-59 pubmed
  3. Unsal E, Degirmenci B, Harmanda B, Erman B, Ozlu N. A small molecule identified through an in silico screen inhibits Aurora B-INCENP interaction. Chem Biol Drug Des. 2016;88:783-794 pubmed publisher
  4. Gholkar A, Cheung K, Williams K, Lo Y, Hamideh S, Nnebe C, et al. Fatostatin Inhibits Cancer Cell Proliferation by Affecting Mitotic Microtubule Spindle Assembly and Cell Division. J Biol Chem. 2016;291:17001-8 pubmed publisher
  5. McKenzie C, Bassi Z, Debski J, Gottardo M, Callaini G, Dadlez M, et al. Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis. Open Biol. 2016;6: pubmed publisher
  6. Lee S, Jeong A, Park J, Han S, Jang C, Kim K, et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell Mol Life Sci. 2016;73:3375-86 pubmed publisher
  7. Baron A, von Schubert C, Cubizolles F, Siemeister G, Hitchcock M, Mengel A, et al. Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524. elife. 2016;5: pubmed publisher
  8. Vazquez Martin A, Sauri Nadal T, Menendez O, Oliveras Ferraros C, Cufí S, Corominas Faja B, et al. Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis. Cell Cycle. 2012;11:4211-21 pubmed publisher
  9. Hebras C, McDougall A. Urochordate ascidians possess a single isoform of Aurora kinase that localizes to the midbody via TPX2 in eggs and cleavage stage embryos. PLoS ONE. 2012;7:e45431 pubmed publisher
  10. Cho H, Shimazu T, Toyokawa G, Daigo Y, Maehara Y, Hayami S, et al. Enhanced HSP70 lysine methylation promotes proliferation of cancer cells through activation of Aurora kinase B. Nat Commun. 2012;3:1072 pubmed publisher
  11. Wang E, Ballister E, Lampson M. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol. 2011;194:539-49 pubmed publisher