This is a Validated Antibody Database (VAD) review about human IL12B, based on 26 published articles (read how Labome selects the articles), using IL12B antibody in all methods. It is aimed to help Labome visitors find the most suited IL12B antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IL12B synonym: CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2; interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; natural killer cell stimulatory factor, 40 kD subunit

Invitrogen
mouse monoclonal (C8.6)
  • blocking or activating experiments; human; 10 ug/ml; fig 3
Invitrogen IL12B antibody (eBioscience, C8.6) was used in blocking or activating experiments on human samples at 10 ug/ml (fig 3). J Immunol (2015) ncbi
mouse monoclonal (C8.6)
  • blocking or activating experiments; human; 2.5 ug/ml
Invitrogen IL12B antibody (eBioscience, C8.6) was used in blocking or activating experiments on human samples at 2.5 ug/ml. J Immunol (2014) ncbi
mouse monoclonal (C8.6)
  • blocking or activating experiments; human
Invitrogen IL12B antibody (eBioscience, C8.6) was used in blocking or activating experiments on human samples . Eur J Immunol (2015) ncbi
mouse monoclonal (C8.6)
  • blocking or activating experiments; human; 10 ug/ml
In order to investigate the anti-influenza responses of T cell subsets, Invitrogen IL12B antibody (eBioscience, C8.6) was used in blocking or activating experiments on human samples at 10 ug/ml. PLoS ONE (2014) ncbi
mouse monoclonal (C8.6)
  • flow cytometry; human; 1 ug/ml; fig 3
Invitrogen IL12B antibody (eBioscience, C8.6) was used in flow cytometry on human samples at 1 ug/ml (fig 3). PLoS ONE (2013) ncbi
mouse monoclonal (C8.6)
  • blocking or activating experiments; human
In order to analyze the governing of human NK-cell activation by TLR-7 and TLR-8 agonists by distinct indirect pathways, Invitrogen IL12B antibody (eBioscience, C8.6) was used in blocking or activating experiments on human samples . Int Immunol (2006) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human; fig 2
In order to characterize human plasmacytoid dendritic cells from breast cancer patients, Invitrogen IL12B antibody (Biosource, C11.5) was used in flow cytometry on human samples (fig 2). J Leukoc Biol (2005) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; Rhesus monkey
  • immunocytochemistry; Rhesus monkey
In order to examine IL-12 and IL-10 production by neutrophils isolated from naive or measles virus-infected rhesus macaques, Invitrogen IL12B antibody (BioSource, C11.5) was used in flow cytometry on Rhesus monkey samples and in immunocytochemistry on Rhesus monkey samples . Viral Immunol (2003) ncbi
rat monoclonal (20C2)
  • ELISA; human; tbl 1
In order to compare the effect of different immunosuppressive regimens on cytokine expression in the grafted lung, Invitrogen IL12B antibody (Endogen, 20C2) was used in ELISA on human samples (tbl 1). Transplant Proc (2003) ncbi
mouse monoclonal (C8.6)
  • ELISA; human; tbl 1
In order to compare the effect of different immunosuppressive regimens on cytokine expression in the grafted lung, Invitrogen IL12B antibody (Endogen, C8.6) was used in ELISA on human samples (tbl 1). Transplant Proc (2003) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; Rhesus monkey
In order to investigate the role of IL-12 in measles induced immunosuppression, Invitrogen IL12B antibody (BioSource International, clone C11.5) was used in flow cytometry on Rhesus monkey samples . J Infect Dis (2002) ncbi
BioLegend
mouse monoclonal (C11.5)
  • flow cytometry; human; loading ...; fig 2b
In order to study the involvement of RANKL in decidual M2 macrophage polarization, BioLegend IL12B antibody (Biolegend, 501807) was used in flow cytometry on human samples (fig 2b). Cell Death Dis (2017) ncbi
mouse monoclonal (C11.5)
  • ELISA; human; loading ...; fig 3b
BioLegend IL12B antibody (BioLegend, C11.5) was used in ELISA on human samples (fig 3b). J Immunol (2017) ncbi
mouse monoclonal (C8.6)
  • blocking or activating experiments; human; 5 ug/ml; fig 3e
BioLegend IL12B antibody (BioLegend, C8.6) was used in blocking or activating experiments on human samples at 5 ug/ml (fig 3e). J Exp Med (2016) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human; loading ...; fig S2
BioLegend IL12B antibody (BioLegend, C11.5) was used in flow cytometry on human samples (fig S2). PLoS ONE (2015) ncbi
rat monoclonal (7B12)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend IL12B antibody (Biolegend, 511002) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human
In order to investigate the progression of innate responses to mycobacteria in infants, BioLegend IL12B antibody (Biolegend, C11.5) was used in flow cytometry on human samples . J Immunol (2014) ncbi
Bio-Rad
mouse monoclonal (CC326)
  • ELISA; cow; loading ...; fig 4d
In order to investigate the effect of Fasciola hepatica FhTLM on host immune functions, Bio-Rad IL12B antibody (AbD Serotec, CC326) was used in ELISA on cow samples (fig 4d). PLoS Pathog (2016) ncbi
mouse monoclonal (CC326)
  • ELISA; sheep
Bio-Rad IL12B antibody (Serotec, MCA 2173B) was used in ELISA on sheep samples . Cell Tissue Res (2014) ncbi
mouse monoclonal (CC301)
  • ELISA; sheep; 8 ug/ml
Bio-Rad IL12B antibody (Serotec, MCA 1782EL) was used in ELISA on sheep samples at 8 ug/ml. Cell Tissue Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (JJ07)
  • western blot; mouse; 1:300; fig 6
Santa Cruz Biotechnology IL12B antibody (Santa Cruz Biotechnology, sc-74147) was used in western blot on mouse samples at 1:300 (fig 6). Sci Rep (2015) ncbi
BD Biosciences
mouse monoclonal (C8.6)
  • flow cytometry; human; fig 2g
In order to map the lineage of human dendritic cells, BD Biosciences IL12B antibody (BD Biosciences, C8.6) was used in flow cytometry on human samples (fig 2g). Science (2017) ncbi
mouse monoclonal (C8.6)
  • immunohistochemistry - frozen section; human
BD Biosciences IL12B antibody (BD Biosciences Pharmingen, C8.6) was used in immunohistochemistry - frozen section on human samples . Tech Coloproctol (2016) ncbi
mouse monoclonal (C8.6)
  • ELISA; human; loading ...; fig 2f
BD Biosciences IL12B antibody (BD Biosciences, C8.6) was used in ELISA on human samples (fig 2f). Cytotherapy (2016) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human; 1:10; loading ...; tbl 2
In order to assess the protective immunological events induced by vaccination with Leishmune in dogs, BD Biosciences IL12B antibody (BD Biosciences, 554575) was used in flow cytometry on human samples at 1:10 (tbl 2). Vet Parasitol (2016) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; dog; fig 4
BD Biosciences IL12B antibody (BD, 554575) was used in flow cytometry on dog samples (fig 4). Acta Vet Scand (2015) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human
BD Biosciences IL12B antibody (BD Biosciences, C11.5) was used in flow cytometry on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human
BD Biosciences IL12B antibody (BD Pharmingen, C11.5) was used in flow cytometry on human samples . J Infect Dis (2015) ncbi
Articles Reviewed
  1. Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
  2. Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
  3. See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  4. Sulaiman A, Zolnierczyk K, Japa O, Owen J, Maddison B, Emes R, et al. A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes. PLoS Pathog. 2016;12:e1005991 pubmed publisher
  5. Tagawa T, Albanese M, Bouvet M, Moosmann A, Mautner J, Heissmeyer V, et al. Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing. J Exp Med. 2016;213:2065-80 pubmed publisher
  6. van Onkelen R, Gosselink M, Van Meurs M, Melief M, Schouten W, Laman J. Pro-inflammatory cytokines in cryptoglandular anal fistulas. Tech Coloproctol. 2016;20:619-25 pubmed publisher
  7. Kolanowski S, van Schijndel G, Van Ham S, ten Brinke A. Adaptation to replating of dendritic cells synergizes with Toll-like receptor stimuli and enhances the pro-inflammatory cytokine profile. Cytotherapy. 2016;18:902-10 pubmed publisher
  8. Moreira M, Costa Pereira C, Alves M, Marteleto B, Ribeiro V, Peruhype Magalhães V, et al. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Vet Parasitol. 2016;220:33-45 pubmed publisher
  9. Moreira M, Dorneles E, Soares R, Magalhães C, Costa Pereira C, Lage A, et al. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining. Acta Vet Scand. 2015;57:51 pubmed publisher
  10. Yang S, He H, Ma Q, Zhang Y, Zhu Y, Wan X, et al. Experimental study of the protective effects of SYVN1 against diabetic retinopathy. Sci Rep. 2015;5:14036 pubmed publisher
  11. Nishibayashi R, Inoue R, Harada Y, Watanabe T, Makioka Y, Ushida K. RNA of Enterococcus faecalis Strain EC-12 Is a Major Component Inducing Interleukin-12 Production from Human Monocytic Cells. PLoS ONE. 2015;10:e0129806 pubmed publisher
  12. Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, et al. Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus. J Immunol. 2015;194:3890-900 pubmed publisher
  13. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  14. Thompson I, Mann E, Stokes M, English N, Knight S, Williamson D. Specific activation of dendritic cells enhances clearance of Bacillus anthracis following infection. PLoS ONE. 2014;9:e109720 pubmed publisher
  15. Boltjes A, van Montfoort N, Biesta P, Op den Brouw M, Kwekkeboom J, van der Laan L, et al. Kupffer cells interact with hepatitis B surface antigen in vivo and in vitro, leading to proinflammatory cytokine production and natural killer cell function. J Infect Dis. 2015;211:1268-78 pubmed publisher
  16. Lim D, Yawata N, Selva K, Li N, Tsai C, Yeong L, et al. The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus. J Immunol. 2014;193:5065-75 pubmed publisher
  17. de Groen R, Boltjes A, Hou J, Liu B, McPhee F, Friborg J, et al. IFN-λ-mediated IL-12 production in macrophages induces IFN-γ production in human NK cells. Eur J Immunol. 2015;45:250-9 pubmed publisher
  18. Deng N, Weaver J, Mosmann T. Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFN?- Thpp cells. PLoS ONE. 2014;9:e95986 pubmed publisher
  19. Shey M, Nemes E, Whatney W, de Kock M, Africa H, Barnard C, et al. Maturation of innate responses to mycobacteria over the first nine months of life. J Immunol. 2014;192:4833-43 pubmed publisher
  20. Jimbo S, Griebel P, Babiuk L, Mutwiri G. IL-10 secreting CD21? B cells are present in jejunal Peyer's patches of sheep during fetal development. Cell Tissue Res. 2014;356:417-25 pubmed publisher
  21. Nagy L, Grishina I, Macal M, Hirao L, Hu W, Sankaran Walters S, et al. Chronic HIV infection enhances the responsiveness of antigen presenting cells to commensal Lactobacillus. PLoS ONE. 2013;8:e72789 pubmed publisher
  22. Gorski K, Waller E, Bjornton Severson J, Hanten J, Riter C, Kieper W, et al. Distinct indirect pathways govern human NK-cell activation by TLR-7 and TLR-8 agonists. Int Immunol. 2006;18:1115-26 pubmed
  23. Cox K, North M, Burke M, Singhal H, Renton S, Aqel N, et al. Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes. J Leukoc Biol. 2005;78:1142-52 pubmed
  24. Hoffman S, Polack F, Hauer D, Griffin D. Measles virus infection of rhesus macaques affects neutrophil expression of IL-12 and IL-10. Viral Immunol. 2003;16:369-79 pubmed
  25. Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, et al. Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection. Transplant Proc. 2003;35:1523-6 pubmed
  26. Polack F, Hoffman S, Moss W, Griffin D. Altered synthesis of interleukin-12 and type 1 and type 2 cytokinesin rhesus macaques during measles and atypical measles. J Infect Dis. 2002;185:13-9 pubmed