This is a Validated Antibody Database (VAD) review about human IL-6R, based on 18 published articles (read how Labome selects the articles), using IL-6R antibody in all methods. It is aimed to help Labome visitors find the most suited IL-6R antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IL-6R synonym: CD126; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ; gp80; interleukin-6 receptor subunit alpha; CD126 antigen; IL-6 receptor subunit alpha; IL-6R 1; membrane glycoprotein 80

Invitrogen
mouse monoclonal (B-R6)
  • blocking or activating experiments; human; tbl 1
In order to investigate the role of hepcidin in the anemia of multiple myeloma, Invitrogen IL-6R antibody (Biosource, B-R6) was used in blocking or activating experiments on human samples (tbl 1). Clin Cancer Res (2008) ncbi
mouse monoclonal (B-R6)
  • blocking or activating experiments; human
In order to establish that IFNalpha treatment results in reduced STAT3 activity in two myeloma cell lines, U266-1984 and U-1958, Invitrogen IL-6R antibody (Biosource, B-R6) was used in blocking or activating experiments on human samples . Exp Cell Res (2007) ncbi
mouse monoclonal (B-R6)
  • flow cytometry; human; fig 1
In order to evaluate the effects of retinoic acid on IL-6 signaling in lymphoblastoid B-cell lines, Invitrogen IL-6R antibody (BioSource, B-R6) was used in flow cytometry on human samples (fig 1). Int J Oncol (2004) ncbi
mouse monoclonal (B-R6)
  • flow cytometry; human
In order to assess the effects of cytokines on integrin expression and mast cell adhesion, Invitrogen IL-6R antibody (Biosource, B-R6) was used in flow cytometry on human samples . J Invest Dermatol (2003) ncbi
mouse monoclonal (B-N12)
  • flow cytometry; human; fig 1
In order to assess the antitumor effects of IL-6 on hepatocarcinoma HepG2 cells, Invitrogen IL-6R antibody (Biosource, clone B-N12) was used in flow cytometry on human samples (fig 1). Eur J Cancer (2001) ncbi
mouse monoclonal (B-N12)
  • flow cytometry; human; fig 2
In order to examine the role of role of neutrophil-derived serine proteases in traumatic brain injuries, Invitrogen IL-6R antibody (Biosource, B-N12) was used in flow cytometry on human samples (fig 2). J Interferon Cytokine Res (1999) ncbi
mouse monoclonal (B-R6)
  • flow cytometry; human; fig 2
In order to examine the role of role of neutrophil-derived serine proteases in traumatic brain injuries, Invitrogen IL-6R antibody (Biosource, B-R-6) was used in flow cytometry on human samples (fig 2). J Interferon Cytokine Res (1999) ncbi
mouse monoclonal (B-R6)
  • ELISA; human; fig 1
In order to study human herpesvirus 8 interleukin 6, Invitrogen IL-6R antibody (Biosource, clone B-R6) was used in ELISA on human samples (fig 1). AIDS (1999) ncbi
Abcam
rat monoclonal (AP-MAB0848)
  • ELISA; human; 1:100; loading ...; fig 7d
  • western blot; mouse; loading ...; fig 2f
Abcam IL-6R antibody (Abcam, ab83053) was used in ELISA on human samples at 1:100 (fig 7d) and in western blot on mouse samples (fig 2f). Biomed Res Int (2017) ncbi
rat monoclonal (AP-MAB0848)
  • western blot; mouse; fig 1a
Abcam IL-6R antibody (Abcam, AB83053) was used in western blot on mouse samples (fig 1a). Biol Sex Differ (2016) ncbi
BioLegend
mouse monoclonal (UV4)
  • flow cytometry; human; loading ...; fig 4b
BioLegend IL-6R antibody (BioLegend, 352805) was used in flow cytometry on human samples (fig 4b). Cancer Res (2016) ncbi
mouse monoclonal (UV4)
  • flow cytometry; human; loading ...; fig 1a
In order to characterize innate lymphoid cell subpopulations isolated from patients with systemic sclerosis, BioLegend IL-6R antibody (biolegend, UV4) was used in flow cytometry on human samples (fig 1a). J Immunol (2016) ncbi
mouse monoclonal (UV4)
  • flow cytometry; human; 1:50
BioLegend IL-6R antibody (BioLegend, 352803) was used in flow cytometry on human samples at 1:50. Mol Cancer Res (2015) ncbi
R&D Systems
mouse monoclonal (17506)
  • blocking or activating experiments; human; 1 ug/ml; loading ...; fig 4f
R&D Systems IL-6R antibody (R&D Systems, 17506) was used in blocking or activating experiments on human samples at 1 ug/ml (fig 4f). J Immunol (2017) ncbi
LifeSpan Biosciences
mouse monoclonal (B-R6)
  • immunohistochemistry - frozen section; human
LifeSpan Biosciences IL-6R antibody (LifeSpan BioSciences, LS-C45194) was used in immunohistochemistry - frozen section on human samples . Clin Exp Immunol (2012) ncbi
Beckman Coulter
mouse monoclonal (M91)
  • flow cytometry; human; fig s4
In order to assess the effects of lenalidomide on normal human plasma cell generation, Beckman Coulter IL-6R antibody (Beckman Coulter, M91) was used in flow cytometry on human samples (fig s4). Oncotarget (2016) ncbi
BD Biosciences
mouse monoclonal (M5)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, BD Biosciences IL-6R antibody (BD Pharmingen, M5) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (M5)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences IL-6R antibody (BD, 551850) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (M5)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences IL-6R antibody (BD, M5) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
Articles Reviewed
  1. Jiao T, Yao Y, Zhang B, Hao D, Sun Q, Li J, et al. Role of MicroRNA-103a Targeting ADAM10 in Abdominal Aortic Aneurysm. Biomed Res Int. 2017;2017:9645874 pubmed publisher
  2. Li R, Rezk A, Li H, Gommerman J, Prat A, Bar Or A. Antibody-Independent Function of Human B Cells Contributes to Antifungal T Cell Responses. J Immunol. 2017;198:3245-3254 pubmed publisher
  3. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  4. Jourdan M, Cren M, Schafer P, Robert N, Duperray C, Vincent L, et al. Differential effects of lenalidomide during plasma cell differentiation. Oncotarget. 2016;7:28096-111 pubmed publisher
  5. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  6. Mishra V, DiAngelo S, Silveyra P. Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation. Biol Sex Differ. 2016;7:16 pubmed publisher
  7. Eichten A, Su J, Adler A, Zhang L, Ioffe E, Parveen A, et al. Resistance to Anti-VEGF Therapy Mediated by Autocrine IL6/STAT3 Signaling and Overcome by IL6 Blockade. Cancer Res. 2016;76:2327-39 pubmed publisher
  8. Roan F, Stoklasek T, Whalen E, Molitor J, Bluestone J, Buckner J, et al. CD4+ Group 1 Innate Lymphoid Cells (ILC) Form a Functionally Distinct ILC Subset That Is Increased in Systemic Sclerosis. J Immunol. 2016;196:2051-2062 pubmed publisher
  9. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  10. Perrigue P, Silva M, Warden C, Feng N, Reid M, Mota D, et al. The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines. Mol Cancer Res. 2015;13:636-50 pubmed publisher
  11. Suttle M, Nilsson G, Snellman E, Harvima I. Experimentally induced psoriatic lesion associates with interleukin (IL)-6 in mast cells and appearance of dermal cells expressing IL-33 and IL-6 receptor. Clin Exp Immunol. 2012;169:311-9 pubmed publisher
  12. Sharma S, Nemeth E, Chen Y, Goodnough J, Huston A, Roodman G, et al. Involvement of hepcidin in the anemia of multiple myeloma. Clin Cancer Res. 2008;14:3262-7 pubmed publisher
  13. Thyrell L, Arulampalam V, Hjortsberg L, Farnebo M, Grander D, Pokrovskaja Tamm K. Interferon alpha induces cell death through interference with interleukin 6 signaling and inhibition of STAT3 activity. Exp Cell Res. 2007;313:4015-24 pubmed
  14. Zancai P, Cariati R, Quaia M, Guidoboni M, Rizzo S, Boiocchi M, et al. Retinoic acid inhibits IL-6-dependent but not constitutive STAT3 activation in Epstein-Barr virus-immortalized B lymphocytes. Int J Oncol. 2004;25:345-55 pubmed
  15. Schoeler D, Grützkau A, Henz B, Küchler J, Kruger Krasagakis S. Interleukin-6 enhances whereas tumor necrosis factor alpha and interferons inhibit integrin expression and adhesion of human mast cells to extracellular matrix proteins. J Invest Dermatol. 2003;120:795-801 pubmed
  16. Cervello M, Notarbartolo M, Landino M, Cusimano A, Virruso L, Montalto G, et al. Downregulation of wild-type beta-catenin expression by interleukin 6 in human hepatocarcinoma HepG2 cells: a possible role in the growth-regulatory effects of the cytokine?. Eur J Cancer. 2001;37:512-9 pubmed
  17. Bank U, Reinhold D, Schneemilch C, Kunz D, Synowitz H, Ansorge S. Selective proteolytic cleavage of IL-2 receptor and IL-6 receptor ligand binding chains by neutrophil-derived serine proteases at foci of inflammation. J Interferon Cytokine Res. 1999;19:1277-87 pubmed
  18. Gage J, Breen E, Echeverri A, Magpantay L, Kishimoto T, Miles S, et al. Human herpesvirus 8-encoded interleukin 6 activates HIV-1 in the U1 monocytic cell line. AIDS. 1999;13:1851-5 pubmed