This is a Validated Antibody Database (VAD) review about human IL-4, based on 67 published articles (read how Labome selects the articles), using IL-4 antibody in all methods. It is aimed to help Labome visitors find the most suited IL-4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IL-4 synonym: BCGF-1; BCGF1; BSF-1; BSF1; IL-4
others
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| IL-4 antibody (Biolegend, 8D4-8) was used in flow cytometry on human samples (fig 7b). Front Pharmacol (2019) ncbi |
Invitrogen
domestic rabbit polyclonal |
| Invitrogen IL-4 antibody (Thermo, pa5-25165) was used in immunohistochemistry on mouse samples at 1:300 (fig 6a). Aging Dis (2021) ncbi |
mouse monoclonal (E10021) |
| In order to develop and characterize a functional and de-immunized version of hyperglycosylated interferon alpha, Invitrogen IL-4 antibody (Endogen, E10021) was used in ELISA on human samples (fig 6b). Clin Immunol (2017) ncbi |
mouse monoclonal (E10023) |
| In order to develop and characterize a functional and de-immunized version of hyperglycosylated interferon alpha, Invitrogen IL-4 antibody (Endogen, E10023) was used in ELISA on human samples at 1.5 ug/ml (fig 6b). Clin Immunol (2017) ncbi |
mouse monoclonal (8D4-8) |
| In order to investigate the roles of CD16+ monocytes in T-cell activation and B-cell responses in systemic lupus erythematosus, Invitrogen IL-4 antibody (eBioscience, 12-7049-42) was used in flow cytometry on human samples (fig 4b). Front Immunol (2016) ncbi |
domestic rabbit polyclonal |
| In order to explore the contribution of STAT6 phosphorylation to Graves' disease and study its role in thyroid epithelial cells, Invitrogen IL-4 antibody (Thermo Fisher Scientific, PA5-25165) was used in immunohistochemistry - paraffin section on mouse samples (fig 4d). Cell Death Dis (2016) ncbi |
mouse monoclonal (8D4-8) |
| Invitrogen IL-4 antibody (eBiosciences, 8D4-8) was used in flow cytometry on human samples (fig 8d). Nat Immunol (2016) ncbi |
mouse monoclonal (8D4-8) |
| Invitrogen IL-4 antibody (eBioscience, 8D4-8) was used in flow cytometry on human samples (fig 2a). PLoS Pathog (2016) ncbi |
mouse monoclonal (8D4-8) |
| Invitrogen IL-4 antibody (Ebioscience, 8D4-8) was used in flow cytometry on human samples . Clin Immunol (2015) ncbi |
mouse monoclonal (8D4-8) |
| Invitrogen IL-4 antibody (eBioscience, 8D4-8) was used in immunocytochemistry on human samples . Autoimmun Rev (2015) ncbi |
mouse monoclonal (8D4-8) |
| Invitrogen IL-4 antibody (eBioscience, 8D4-8) was used in flow cytometry on human samples . J Allergy Clin Immunol (2015) ncbi |
mouse monoclonal (8D4-8) |
| Invitrogen IL-4 antibody (eBioscience, 8D48) was used in flow cytometry on human samples . PLoS ONE (2014) ncbi |
rat monoclonal (MP4-25D2) |
| In order to investigate the anti-influenza responses of T cell subsets, Invitrogen IL-4 antibody (eBioscience, MP4-25D2) was used in blocking or activating experiments on human samples at 10 ug/ml. PLoS ONE (2014) ncbi |
rat monoclonal (MP4-25D2) |
| In order to assess how different concentrations of IFN-gamma affect dendritic cells, Invitrogen IL-4 antibody (Invitrogen Dynal AS, MP425D2) was used in flow cytometry on human samples (fig 7b). J Leukoc Biol (2014) ncbi |
rat monoclonal (MP4-25D2) |
| In order to use semantic connectivity maps to find biomarkers for multiple sclerosis, Invitrogen IL-4 antibody (eBioscience, MP4-25D2) was used in flow cytometry on human samples . Immun Ageing (2013) ncbi |
rat monoclonal (25D2) |
| In order to determine the localization of CD4 positive T cells subsets in the healthy intestine, Invitrogen IL-4 antibody (Invitrogen, MP4-25D2) was used in flow cytometry on human samples at 1:50. PLoS ONE (2012) ncbi |
mouse monoclonal (860A4B3) |
| In order to examine how antipsychotic drugs affect immune responses, Invitrogen IL-4 antibody (Invitrogen, 4B3) was used in blocking or activating experiments on human samples at 5 ug/ml. Immunol Lett (2011) ncbi |
rat monoclonal (MP4-25D2) |
| In order to quantify the prevalence of Th1 and Th17 cells in the joints of rheumatoid arthritis patients, Invitrogen IL-4 antibody (e-Bioscience, MP4-25D2) was used in flow cytometry on human samples (fig 1C). Ann Rheum Dis (2008) ncbi |
rat monoclonal (25D2) |
| In order to develop a system for the decoration of enveloped viruses with functionally active cytokines, Invitrogen IL-4 antibody (Caltag, MP4-25D2) was used in immunocytochemistry on human samples . J Virol (2007) ncbi |
rat monoclonal (MP4-25D2) |
| In order to study GATA-3 and T-box expression in human T cells, Invitrogen IL-4 antibody (eBioscience, MP4-25D2) was used in blocking or activating experiments on human samples at 5 ug/ml. Blood (2007) ncbi |
mouse monoclonal (860A4B3) |
| In order to compare the ability of plasmacytoid dendritic cells and monocyte-derived dendritic cells to activate T cells, Invitrogen IL-4 antibody (Biosource, 860A4B3) was used in ELISA on human samples . Immunology (2006) ncbi |
rat monoclonal (25D2) |
| In order to use flow cytometry to study granzyme A, B, and K expression in human peripheral blood lymphocytes, Invitrogen IL-4 antibody (Caltag, MP4-25D2) was used in flow cytometry on human samples (tbl 2). Eur J Immunol (2005) ncbi |
rat monoclonal (25D2) |
| In order to show that human rhinoviruses alters the accessory molecule repertoire of dendritic cells, Invitrogen IL-4 antibody (Caltag, MP4-25D2) was used in flow cytometry on human samples (fig 2). J Immunol (2005) ncbi |
mouse monoclonal (860A4B3) |
| In order to explore the immunomodulatory effects of insulin-like growth factor through regulation of cytokine production, Invitrogen IL-4 antibody (Biosource, 860A4B3) was used in ELISA on human samples at 1 ug/ml (tbl 1). J Leukoc Biol (2004) ncbi |
mouse monoclonal (860A4B3) |
| In order to describe the phenotype and function of Valpha24(+) splenic T cells from rhesus macaques, Invitrogen IL-4 antibody (Biosource, 860A 4B3) was used in flow cytometry on rhesus macaque samples (fig 4, 5). J Immunol (2003) ncbi |
rat monoclonal (25D2) |
| In order to investigate the involvement of the PD-1 system in rheumatoid arthritis, Invitrogen IL-4 antibody (Caltag, MP4-25D2) was used in flow cytometry on human samples (fig 5). J Rheumatol (2003) ncbi |
mouse monoclonal (860A4B3) |
| In order to report an in vitro differentiation assay in which human naive CD4(+) cells are driven toward either the Th1 or Th2 phenotype and study them, Invitrogen IL-4 antibody (BioSource, 4B3) was used in flow cytometry on human samples (fig 1). J Immunol (2002) ncbi |
mouse monoclonal (860F10H12) |
| In order to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines, Invitrogen IL-4 antibody (Biosource, 860F10H12) was used in ELISA on human samples (fig 2, 3). Int Immunol (2001) ncbi |
mouse monoclonal (860A4B3) |
| In order to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines, Invitrogen IL-4 antibody (Biosource, 860A4B3) was used in ELISA on human samples (fig 2, 3). Int Immunol (2001) ncbi |
BioLegend
rat monoclonal (MP4-25D2) |
| BioLegend IL-4 antibody (Biolegend, MP4-25D2) was used in mass cytometry on human samples (fig s1a). Acta Neuropathol (2021) ncbi |
rat monoclonal (MP4-25D2) |
| BioLegend IL-4 antibody (Biolegend, 500824) was used in flow cytometry on human samples (fig s6d). Cell (2021) ncbi |
rat monoclonal (MP4-25D2) |
| BioLegend IL-4 antibody (BioLegend, MP4-25D2) was used in flow cytometry on mouse samples (fig 5a). Science (2019) ncbi |
mouse monoclonal (8D4-8) |
| BioLegend IL-4 antibody (BioLegend, 8D4-8) was used in flow cytometry on human samples (fig 1b). J Immunol (2019) ncbi |
rat monoclonal (MP4-25D2) |
| BioLegend IL-4 antibody (BioLegend, 500828) was used in flow cytometry on human samples (fig 1e). Cell (2019) ncbi |
mouse monoclonal (8D4-8) |
| BioLegend IL-4 antibody (Biolegend, 8D4-8) was used in flow cytometry on human samples (fig 7b). Front Pharmacol (2019) ncbi |
rat monoclonal (MP4-25D2) |
| In order to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment, BioLegend IL-4 antibody (Biolegend, MP4-25D2) was used in flow cytometry on human samples (tbl 1). Cytometry A (2017) ncbi |
rat monoclonal (MP4-25D2) |
| In order to demonstrate that TGF-beta induces the differentiation of human CXCL13-producing CD4 positive T cells from naive CD4 positive T cells, BioLegend IL-4 antibody (BioLegend, MP4-25D2) was used in blocking or activating experiments on human samples . Eur J Immunol (2016) ncbi |
mouse monoclonal (8D4-8) |
| BioLegend IL-4 antibody (BioLegend, 8D4-8) was used in flow cytometry on human samples . Thromb Res (2015) ncbi |
rat monoclonal (MP4-25D2) |
| In order to analyze mucosal pinch biopsies collected predominantly during colonoscopies, BioLegend IL-4 antibody (Biolegend, MP4-25D2) was used in flow cytometry on human samples at 2 ul/test. J Immunol Methods (2015) ncbi |
rat monoclonal (MP4-25D2) |
| In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend IL-4 antibody (Biolegend, 500802) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi |
mouse monoclonal (8D4-8) |
| In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend IL-4 antibody (Biolegend, 500702) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi |
rat monoclonal (MP4-25D2) |
| In order to study the role of dendritic cells in relation to T cells, BioLegend IL-4 antibody (BioLegend, MP4-25D2) was used in immunocytochemistry on human samples . J Immunol (2014) ncbi |
rat monoclonal (MP4-25D2) |
| BioLegend IL-4 antibody (Biolegend, MP4-25D2) was used in flow cytometry on human samples . Med Microbiol Immunol (2014) ncbi |
R&D Systems
mouse monoclonal (34019) |
| R&D Systems IL-4 antibody (R&D Systems, MAB204) was used in blocking or activating experiments on human samples (fig 8a). iScience (2021) ncbi |
Abcam
domestic rabbit polyclonal |
| In order to discuss mutations that contribute to atopic dermatitis, Abcam IL-4 antibody (Abcam, ab9622) was used in western blot on rat samples at 1:500 (fig s1b). J Allergy Clin Immunol (2017) ncbi |
domestic rabbit polyclonal |
| Abcam IL-4 antibody (abcam, ab9622) was used in immunohistochemistry on mouse samples at 1:100 (fig 4). J Neuroinflammation (2016) ncbi |
domestic rabbit polyclonal |
| Abcam IL-4 antibody (Abcam, ab9622) was used in immunohistochemistry on human samples at 1:200 (fig 4). Arch Med Sci (2016) ncbi |
Beckman Coulter
mouse monoclonal (4D9) |
| Beckman Coulter IL-4 antibody (Beckman Coulter, IM2719U) was used in flow cytometry on human samples (fig 1). Exp Ther Med (2017) ncbi |
Cell Signaling Technology
domestic rabbit monoclonal (D19A10) |
| Cell Signaling Technology IL-4 antibody (Cell Signaling, D19A10) was used in western blot on human samples at 1:1000 (fig 3b). PLoS ONE (2018) ncbi |
BD Biosciences
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Biosciences, 559333) was used in flow cytometry on human samples (fig 8a). iScience (2021) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD, 8D4-8) was used in flow cytometry on mouse samples (fig 4). BMC Infect Dis (2019) ncbi |
rat monoclonal (MP4-25D2) |
| BD Biosciences IL-4 antibody (BD Biosciences, 554481) was used in blocking or activating experiments on human samples at 0.01 ug/ml (fig 6a). elife (2019) ncbi |
rat monoclonal (MP4-25D2) |
| BD Biosciences IL-4 antibody (BD, MP4-25D2) was used in flow cytometry on human samples (fig 3b). Infect Immun (2019) ncbi |
rat monoclonal (MP4-25D2) |
| BD Biosciences IL-4 antibody (BD Biosciences, MP4-25D2) was used in flow cytometry on human samples (fig s3b). Int J Hematol (2018) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Biosciences, 8D4-8) was used in flow cytometry on human samples (fig 1e). J Immunol (2018) ncbi |
rat monoclonal (MP4-25D2) |
| BD Biosciences IL-4 antibody (BD Biosciences, 554484) was used in flow cytometry on human samples (tbl 1). J Clin Invest (2018) ncbi |
mouse (8D4-8) |
| BD Biosciences IL-4 antibody (BD Bioscience, 8D4-8) was used in flow cytometry on human samples (fig 6a). PLoS ONE (2017) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Biosciences, 8D4-8) was used in flow cytometry on rhesus macaque samples (fig 5a). JCI Insight (2017) ncbi |
rat monoclonal (MP4-25D2) |
| In order to optimize and assess potential malaria vaccine regimens, BD Biosciences IL-4 antibody (BD, 564110) was used in flow cytometry on human samples (tbl s9). Nature (2017) ncbi |
mouse monoclonal (8D4-8) |
| In order to evaluate combinations of TLR and C-type lectin receptor agonists on the Th1 responses of newborn dendritic cells, BD Biosciences IL-4 antibody (BD Biosciences, 8D4-8) was used in flow cytometry on human samples (fig s2b). J Immunol (2016) ncbi |
rat monoclonal (MP4-25D2) |
| BD Biosciences IL-4 antibody (BD Biosciences, 554481) was used in blocking or activating experiments on human samples (fig s3c). Cell (2016) ncbi |
mouse monoclonal (8D4-8) |
| In order to report that IgA-producing plasma cells are a major source of inducible NO synthase in Helicobacter pylori-infected patients, BD Biosciences IL-4 antibody (BD, 8D4-8) was used in flow cytometry on human samples . J Immunol (2016) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Pharmingen, 8D4-8) was used in flow cytometry on human samples (fig 3d). PLoS ONE (2016) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD, 561234) was used in flow cytometry on human samples (fig 8). Nat Commun (2015) ncbi |
rat monoclonal (MP4-25D2) |
| BD Biosciences IL-4 antibody (BD Biosciences, 554486) was used in flow cytometry on human samples at 1:200 (fig 5). Nat Commun (2015) ncbi |
rat monoclonal (MP4-25D2) |
| In order to show that hepatitis B virus exposure in utero enhances immunity to bacterial infections, BD Biosciences IL-4 antibody (Becton Dickinson, MP4-25D2) was used in flow cytometry on human samples at 4:100 (fig 3a). Nat Commun (2015) ncbi |
mouse monoclonal (8D4-8) |
| In order to discuss how inflammation contributes to pulmonary arterial hypertension, BD Biosciences IL-4 antibody (BD Pharmingen, 8D4-8) was used in flow cytometry on human samples . Chest (2015) ncbi |
mouse monoclonal (3010.211) |
| In order to examine the role of TLR7 in HIV-infected CD4 positive T cells, BD Biosciences IL-4 antibody (BD, 3010.211) was used in flow cytometry on human samples . Nat Immunol (2015) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Biosciences, 8D4-8) was used in flow cytometry on human samples (fig 5). Nat Immunol (2014) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Pharmingen, 8D4-8) was used in flow cytometry on human samples . PLoS ONE (2014) ncbi |
mouse monoclonal (3010.211) |
| BD Biosciences IL-4 antibody (BD, 3010.211) was used in flow cytometry on human samples . J Exp Med (2014) ncbi |
mouse monoclonal (8D4-8) |
| BD Biosciences IL-4 antibody (BD Pharmigen, 8D4-8) was used in flow cytometry on human samples . J Cell Physiol (2014) ncbi |
Articles Reviewed
- Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, et al. CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus. Front Immunol. 2016;7:512 pubmed
- Naeem A, Tommasi C, Cole C, Brown S, Zhu Y, Way B, et al. A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic d. J Allergy Clin Immunol. 2017;139:1228-1241 pubmed publisher
- van Haren S, Dowling D, Foppen W, Christensen D, Andersen P, Reed S, et al. Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization. J Immunol. 2016;197:4413-4424 pubmed
- Galindo Albarrán A, Ramirez Pliego O, Labastida Conde R, Melchy Pérez E, Liquitaya Montiel A, Esquivel Guadarrama F, et al. CD43 signals prepare human T cells to receive cytokine differentiation signals. J Cell Physiol. 2014;229:172-80 pubmed
- Yamada H, Nakashima Y, Okazaki K, Mawatari T, Fukushi J, Kaibara N, et al. Th1 but not Th17 cells predominate in the joints of patients with rheumatoid arthritis. Ann Rheum Dis. 2008;67:1299-304 pubmed
- Kueng H, Leb V, Haiderer D, Raposo G, Thery C, Derdak S, et al. General strategy for decoration of enveloped viruses with functionally active lipid-modified cytokines. J Virol. 2007;81:8666-76 pubmed
- De Fanis U, Mori F, Kurnat R, Lee W, Bova M, Adkinson N, et al. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007;109:4343-50 pubmed
- Janke M, Witsch E, Mages H, Hutloff A, Kroczek R. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60 pubmed
- Bratke K, Kuepper M, Bade B, Virchow J, Luttmann W. Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005;35:2608-16 pubmed
- Kirchberger S, Majdic O, Steinberger P, Bluml S, Pfistershammer K, Zlabinger G, et al. Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J Immunol. 2005;175:1145-52 pubmed
- Kooijman R, Coppens A. Insulin-like growth factor-I stimulates IL-10 production in human T cells. J Leukoc Biol. 2004;76:862-7 pubmed
- Gansuvd B, Hubbard W, Hutchings A, Thomas F, Goodwin J, Wilson S, et al. Phenotypic and functional characterization of long-term cultured rhesus macaque spleen-derived NKT cells. J Immunol. 2003;171:2904-11 pubmed
- Hatachi S, Iwai Y, Kawano S, Morinobu S, Kobayashi M, Koshiba M, et al. CD4+ PD-1+ T cells accumulate as unique anergic cells in rheumatoid arthritis synovial fluid. J Rheumatol. 2003;30:1410-9 pubmed
- Cousins D, Lee T, Staynov D. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506 pubmed
- Bullens D, Rafiq K, Charitidou L, Peng X, Kasran A, Warmerdam P, et al. Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production. Int Immunol. 2001;13:181-91 pubmed
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