This is a Validated Antibody Database (VAD) review about human IL-12, based on 11 published articles (read how Labome selects the articles), using IL-12 antibody in all methods. It is aimed to help Labome visitors find the most suited IL-12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IL-12 synonym: CLMF; IL-12A; NFSK; NKSF1; P35

Invitrogen
domestic rabbit recombinant (6H15L18)
  • western blot; mouse; 1:500; fig 2e
Invitrogen IL-12 antibody (Invitrogen, 701101) was used in western blot on mouse samples at 1:500 (fig 2e). Allergy Asthma Clin Immunol (2021) ncbi
mouse monoclonal (B-T21 (BT21))
  • flow cytometry; human; loading ...; fig 2c
Invitrogen IL-12 antibody (Invitrogen, BT21) was used in flow cytometry on human samples (fig 2c). Aging Cell (2021) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; human; fig 2
In order to characterize human plasmacytoid dendritic cells from breast cancer patients, Invitrogen IL-12 antibody (Biosource, C11.5) was used in flow cytometry on human samples (fig 2). J Leukoc Biol (2005) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; rhesus macaque
  • immunocytochemistry; rhesus macaque
In order to examine IL-12 and IL-10 production by neutrophils isolated from naive or measles virus-infected rhesus macaques, Invitrogen IL-12 antibody (BioSource, C11.5) was used in flow cytometry on rhesus macaque samples and in immunocytochemistry on rhesus macaque samples . Viral Immunol (2003) ncbi
rat monoclonal (20C2)
  • ELISA; human; tbl 1
In order to compare the effect of different immunosuppressive regimens on cytokine expression in the grafted lung, Invitrogen IL-12 antibody (Endogen, 20C2) was used in ELISA on human samples (tbl 1). Transplant Proc (2003) ncbi
mouse monoclonal (C11.5)
  • flow cytometry; rhesus macaque
In order to investigate the role of IL-12 in measles induced immunosuppression, Invitrogen IL-12 antibody (BioSource International, clone C11.5) was used in flow cytometry on rhesus macaque samples . J Infect Dis (2002) ncbi
R&D Systems
mouse monoclonal (24910)
  • blocking or activating experiments; human; 2 ug/ml; loading ...; fig 8a
R&D Systems IL-12 antibody (R&D Systems, MAB219) was used in blocking or activating experiments on human samples at 2 ug/ml (fig 8a). iScience (2021) ncbi
mouse monoclonal (24910)
  • flow cytometry; human; loading ...; fig 2c
R&D Systems IL-12 antibody (RnD Systems, 24916) was used in flow cytometry on human samples (fig 2c). Aging Cell (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (14L7)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7a
Santa Cruz Biotechnology IL-12 antibody (Santa Cruz, 74150) was used in immunohistochemistry - paraffin section on mouse samples (fig 7a). Arthritis Res Ther (2022) ncbi
mouse monoclonal (JJ07)
  • western blot; mouse; 1:300; fig 6
Santa Cruz Biotechnology IL-12 antibody (Santa Cruz Biotechnology, sc-74147) was used in western blot on mouse samples at 1:300 (fig 6). Sci Rep (2015) ncbi
Abcam
domestic rabbit monoclonal (EP5737)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3a
Abcam IL-12 antibody (Abcam, ab131039) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 3a). J Mol Cell Cardiol (2018) ncbi
BioLegend
rat monoclonal (7B12)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend IL-12 antibody (Biolegend, 511002) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
Articles Reviewed
  1. Zhang L, Zheng D, Yan Y, Yu Y, Chen R, Li Z, et al. Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis. Arthritis Res Ther. 2022;24:148 pubmed publisher
  2. Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, et al. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience. 2021;24:103433 pubmed publisher
  3. Zhou J, Lu Y, Wu W, Feng Y. Taurine promotes the production of CD4+CD25+FOXP3+ Treg cells through regulating IL-35/STAT1 pathway in a mouse allergic rhinitis model. Allergy Asthma Clin Immunol. 2021;17:59 pubmed publisher
  4. Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  5. Cao Y, Xu Y, Auchoybur M, Chen W, He S, Qin W, et al. Regulatory role of IKKÉ‘ in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages. J Mol Cell Cardiol. 2018;123:1-12 pubmed publisher
  6. Yang S, He H, Ma Q, Zhang Y, Zhu Y, Wan X, et al. Experimental study of the protective effects of SYVN1 against diabetic retinopathy. Sci Rep. 2015;5:14036 pubmed publisher
  7. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  8. Cox K, North M, Burke M, Singhal H, Renton S, Aqel N, et al. Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes. J Leukoc Biol. 2005;78:1142-52 pubmed
  9. Hoffman S, Polack F, Hauer D, Griffin D. Measles virus infection of rhesus macaques affects neutrophil expression of IL-12 and IL-10. Viral Immunol. 2003;16:369-79 pubmed
  10. Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, et al. Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection. Transplant Proc. 2003;35:1523-6 pubmed
  11. Polack F, Hoffman S, Moss W, Griffin D. Altered synthesis of interleukin-12 and type 1 and type 2 cytokinesin rhesus macaques during measles and atypical measles. J Infect Dis. 2002;185:13-9 pubmed