This is a Validated Antibody Database (VAD) review about human IKKgamma, based on 18 published articles (read how Labome selects the articles), using IKKgamma antibody in all methods. It is aimed to help Labome visitors find the most suited IKKgamma antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IKKgamma synonym: AMCBX1; EDAID1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9

Knockout validation
Cell Signaling Technology
mouse monoclonal (DA10-12)
  • western blot knockout validation; mouse; 1 ug/ml; fig 1
In order to report that NEMO contributes to RIG-I activation in response to viruses, Cell Signaling Technology IKKgamma antibody (Cell Signaling, DA10-12) was used in western blot knockout validation on mouse samples at 1 ug/ml (fig 1). Nat Immunol (2007) ncbi
BD Biosciences
mouse monoclonal (54/IKK?/NEMO)
  • western blot knockout validation; human; fig s4
  • immunocytochemistry; human; fig s1
BD Biosciences IKKgamma antibody (BD Transduction Laboratories, 611306) was used in western blot knockout validation on human samples (fig s4) and in immunocytochemistry on human samples (fig s1). Nat Commun (2016) ncbi
Invitrogen
mouse monoclonal (72C627)
  • western blot; mouse; 1:1000; loading ...; fig 5b
Invitrogen IKKgamma antibody (Invitrogen, MA1-41046) was used in western blot on mouse samples at 1:1000 (fig 5b). Sci Signal (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-3)
  • western blot; human; fig 5
Santa Cruz Biotechnology IKKgamma antibody (Santa Cruz, sc-8032) was used in western blot on human samples (fig 5). Oncotarget (2015) ncbi
mouse monoclonal (B-3)
  • western blot; human
Santa Cruz Biotechnology IKKgamma antibody (Santa Cruz, sc-8032) was used in western blot on human samples . Mol Cell Biol (2013) ncbi
Abcam
domestic rabbit monoclonal (EPR16629)
  • western blot; mouse; 1:5000; fig 7
Abcam IKKgamma antibody (Abcam, ab178872) was used in western blot on mouse samples at 1:5000 (fig 7). Anal Biochem (2016) ncbi
Active Motif
mouse monoclonal (46B844)
  • western blot; rat; 1:500
Active Motif IKKgamma antibody (Active Motif, 40908) was used in western blot on rat samples at 1:500. J Cereb Blood Flow Metab (2003) ncbi
MilliporeSigma
mouse monoclonal (1D4)
  • western blot; human; loading ...; fig 4b
MilliporeSigma IKKgamma antibody (Sigma, SAB1404591) was used in western blot on human samples (fig 4b). Nat Microbiol (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s7e
Cell Signaling Technology IKKgamma antibody (Cell Signaling, 2685) was used in western blot on mouse samples (fig s7e). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3f
Cell Signaling Technology IKKgamma antibody (CST, 2685) was used in western blot on mouse samples (fig 3f). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (DA10-12)
  • western blot; human; loading ...; fig 3d
In order to discover that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex to the IkB kinase complex, Cell Signaling Technology IKKgamma antibody (Cell Signaling, 2695) was used in western blot on human samples (fig 3d). PLoS Pathog (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5b
Cell Signaling Technology IKKgamma antibody (Cell Signaling, 2685) was used in western blot on mouse samples (fig 5b). J Immunol (2016) ncbi
mouse monoclonal (DA10-12)
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology IKKgamma antibody (Cell Signaling, 2695) was used in western blot on mouse samples at 1:1000 (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (DA10-12)
  • western blot; human
Cell Signaling Technology IKKgamma antibody (CST, 2695S) was used in western blot on human samples . Oncotarget (2014) ncbi
mouse monoclonal (DA10-12)
  • western blot knockout validation; mouse; 1 ug/ml; fig 1
In order to report that NEMO contributes to RIG-I activation in response to viruses, Cell Signaling Technology IKKgamma antibody (Cell Signaling, DA10-12) was used in western blot knockout validation on mouse samples at 1 ug/ml (fig 1). Nat Immunol (2007) ncbi
BD Biosciences
mouse monoclonal (54/IKK?/NEMO)
  • western blot knockout validation; human; fig s4
  • immunocytochemistry; human; fig s1
BD Biosciences IKKgamma antibody (BD Transduction Laboratories, 611306) was used in western blot knockout validation on human samples (fig s4) and in immunocytochemistry on human samples (fig s1). Nat Commun (2016) ncbi
mouse monoclonal (54/IKK?/NEMO)
  • western blot; human; 1:2000
BD Biosciences IKKgamma antibody (BD Biosciences, 611306) was used in western blot on human samples at 1:2000. BMC Biol (2016) ncbi
mouse monoclonal (54/IKK?/NEMO)
  • western blot; human; fig 3
BD Biosciences IKKgamma antibody (BD, 611306) was used in western blot on human samples (fig 3). J Exp Med (2015) ncbi
mouse monoclonal (54/IKK?/NEMO)
  • immunocytochemistry; human
In order to study the mechanism by which Tax-2 activates NFkB, BD Biosciences IKKgamma antibody (BD Transduction Laboratories, 611306) was used in immunocytochemistry on human samples . J Virol (2013) ncbi
MBL International
  • western blot; human; 1:1000; fig s15
MBL International IKKgamma antibody (MBL, K0159-3) was used in western blot on human samples at 1:1000 (fig s15). Nat Commun (2016) ncbi
Articles Reviewed
  1. Yang S, Harding A, Sweeney C, Miao D, Swan G, Zhou C, et al. Control of antiviral innate immune response by protein geranylgeranylation. Sci Adv. 2019;5:eaav7999 pubmed publisher
  2. Herb M, Gluschko A, Wiegmann K, Farid A, Wolf A, Utermöhlen O, et al. Mitochondrial reactive oxygen species enable proinflammatory signaling through disulfide linkage of NEMO. Sci Signal. 2019;12: pubmed publisher
  3. Wang H, Meng H, Li X, Zhu K, Dong K, Mookhtiar A, et al. PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP. Proc Natl Acad Sci U S A. 2017;114:11944-11949 pubmed publisher
  4. Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, et al. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. PLoS Pathog. 2017;13:e1006162 pubmed publisher
  5. Scholefield J, Henriques R, Savulescu A, Fontan E, Boucharlat A, Laplantine E, et al. Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti. Nat Commun. 2016;7:12629 pubmed publisher
  6. de Jong M, Liu Z, Chen D, Alto N. Shigella flexneri suppresses NF-?B activation by inhibiting linear ubiquitin chain ligation. Nat Microbiol. 2016;1:16084 pubmed publisher
  7. Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  8. Pourcelot M, Zemirli N, Silva da Costa L, Loyant R, Garcin D, Vitour D, et al. The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing. BMC Biol. 2016;14:69 pubmed publisher
  9. Naik E, Dixit V. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCβ Kinase Activity. J Immunol. 2016;196:3438-51 pubmed publisher
  10. Maria Z, Campolo A, Lacombe V. Diabetes Alters the Expression and Translocation of the Insulin-Sensitive Glucose Transporters 4 and 8 in the Atria. PLoS ONE. 2015;10:e0146033 pubmed publisher
  11. Spencer N, Yan Z, Cong L, Zhang Y, Engelhardt J, Stanton R. Definitive localization of intracellular proteins: Novel approach using CRISPR-Cas9 genome editing, with glucose 6-phosphate dehydrogenase as a model. Anal Biochem. 2016;494:55-67 pubmed publisher
  12. Pulvino M, Chen L, Oleksyn D, Li J, Compitello G, Rossi R, et al. Inhibition of COP9-signalosome (CSN) deneddylating activity and tumor growth of diffuse large B-cell lymphomas by doxycycline. Oncotarget. 2015;6:14796-813 pubmed
  13. Boisson B, Laplantine E, Dobbs K, Cobat A, Tarantino N, Hazen M, et al. Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia. J Exp Med. 2015;212:939-51 pubmed publisher
  14. Zhuang C, Sheng C, Shin W, Wu Y, Li J, Yao J, et al. A novel drug discovery strategy: mechanistic investigation of an enantiomeric antitumor agent targeting dual p53 and NF-κB pathways. Oncotarget. 2014;5:10830-9 pubmed
  15. Chan W, Schaffer T, Pomerantz J. A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival. Mol Cell Biol. 2013;33:429-43 pubmed publisher
  16. Journo C, Bonnet A, Favre Bonvin A, Turpin J, Vinera J, C t E, et al. Human T cell leukemia virus type 2 tax-mediated NF-?B activation involves a mechanism independent of Tax conjugation to ubiquitin and SUMO. J Virol. 2013;87:1123-36 pubmed publisher
  17. Zhao T, Yang L, Sun Q, Arguello M, Ballard D, Hiscott J, et al. The NEMO adaptor bridges the nuclear factor-kappaB and interferon regulatory factor signaling pathways. Nat Immunol. 2007;8:592-600 pubmed
  18. Han H, Karabiyikoglu M, Kelly S, Sobel R, Yenari M. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003;23:589-98 pubmed