This is a Validated Antibody Database (VAD) review about human IKBKE, based on 8 published articles (read how Labome selects the articles), using IKBKE antibody in all methods. It is aimed to help Labome visitors find the most suited IKBKE antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IKBKE synonym: IKK-E; IKK-i; IKKE; IKKI; inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-epsilon; IKK-related kinase epsilon; inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

Knockout validation
Cell Signaling Technology
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 2h
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4e
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 2b
Cell Signaling Technology IKBKE antibody (Cell Signaling, 2690) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 2h), in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 4e), in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 2d) and in western blot on human samples at 1:1000 (fig 2b). Biomed Pharmacother (2019) ncbi
Cell Signaling Technology
rabbit monoclonal (D61F9)
  • western blot knockout validation; mouse; loading ...; fig 2C
Cell Signaling Technology IKBKE antibody (Cell Signaling, 3416) was used in western blot knockout validation on mouse samples (fig 2C). Biochem J (2017) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 2h
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4e
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 2b
Cell Signaling Technology IKBKE antibody (Cell Signaling, 2690) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 2h), in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 4e), in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 2d) and in western blot on human samples at 1:1000 (fig 2b). Biomed Pharmacother (2019) ncbi
rabbit monoclonal (D1B7)
  • western blot; human; 1:1000; loading ...; fig 1a
  • western blot; mouse; 1:1000; loading ...; fig 1b
Cell Signaling Technology IKBKE antibody (Cell Signaling, 8766) was used in western blot on human samples at 1:1000 (fig 1a) and in western blot on mouse samples at 1:1000 (fig 1b). Front Cell Dev Biol (2018) ncbi
rabbit monoclonal (D20G4)
  • immunoprecipitation; human; 1:100; loading ...; fig 7a
  • western blot; human; 1:1000; loading ...; fig 1d
Cell Signaling Technology IKBKE antibody (Cell Signaling, 2905) was used in immunoprecipitation on human samples at 1:100 (fig 7a) and in western blot on human samples at 1:1000 (fig 1d). Oncotarget (2017) ncbi
rabbit monoclonal (D61F9)
  • western blot; mouse; fig 3c
  • western blot; human; loading ...; fig 2b
Cell Signaling Technology IKBKE antibody (Cell Signaling, 3416) was used in western blot on mouse samples (fig 3c) and in western blot on human samples (fig 2b). J Biol Chem (2017) ncbi
rabbit monoclonal (D1B7)
  • western blot; mouse; loading ...; fig 2A
Cell Signaling Technology IKBKE antibody (Cell Signaling, 8766) was used in western blot on mouse samples (fig 2A). Biochem J (2017) ncbi
rabbit monoclonal (D61F9)
  • western blot knockout validation; mouse; loading ...; fig 2C
Cell Signaling Technology IKBKE antibody (Cell Signaling, 3416) was used in western blot knockout validation on mouse samples (fig 2C). Biochem J (2017) ncbi
rabbit monoclonal (D20G4)
  • western blot; human; 1:100; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology IKBKE antibody (Cell Signaling, 2905) was used in western blot on human samples at 1:100 (fig st1). Nat Commun (2016) ncbi
rabbit monoclonal (D1B7)
  • western blot; human; fig 4
Cell Signaling Technology IKBKE antibody (Cell signaling, 8766S) was used in western blot on human samples (fig 4). Nat Immunol (2016) ncbi
rabbit monoclonal (D20G4)
  • western blot; human; fig 4
Cell Signaling Technology IKBKE antibody (Cell signaling, 2905S) was used in western blot on human samples (fig 4). Nat Immunol (2016) ncbi
rabbit monoclonal (D61F9)
  • western blot; mouse
Cell Signaling Technology IKBKE antibody (Cell Signaling Technology, 3416) was used in western blot on mouse samples . PLoS ONE (2013) ncbi
Articles Reviewed
  1. He S, Nian F, Chen W, Yin L, Auchoybur M, Tao Z, et al. I-κB kinase-ε knockout protects against angiotensin II induced aortic valve thickening in apolipoprotein E deficient mice. Biomed Pharmacother. 2019;109:1287-1295 pubmed publisher
  2. Lork M, Kreike M, Staal J, Beyaert R. Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKK?/TBK1 as a Case Study. Front Cell Dev Biol. 2018;6:40 pubmed publisher
  3. Lu J, Yang Y, Guo G, Liu Y, Zhang Z, Dong S, et al. IKBKE regulates cell proliferation and epithelial-mesenchymal transition of human malignant glioma via the Hippo pathway. Oncotarget. 2017;8:49502-49514 pubmed publisher
  4. Hsia H, Hutti J, Baldwin A. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding Kinase 1 (TBK1) to Restrain STAT3 Activity. J Biol Chem. 2017;292:5405-5417 pubmed publisher
  5. Bakshi S, Taylor J, Strickson S, McCartney T, Cohen P. Identification of TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon ?. Biochem J. 2017;474:1163-1174 pubmed publisher
  6. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  7. Starokadomskyy P, Gemelli T, Rios J, Xing C, Wang R, Li H, et al. DNA polymerase-? regulates the activation of type I interferons through cytosolic RNA:DNA synthesis. Nat Immunol. 2016;17:495-504 pubmed publisher
  8. Dai J, Shen D, Bian Z, Zhou H, Gan H, Zong J, et al. IKKi deficiency promotes pressure overload-induced cardiac hypertrophy and fibrosis. PLoS ONE. 2013;8:e53412 pubmed publisher