This is a Validated Antibody Database (VAD) review about human IFT20, based on 5 published articles (read how Labome selects the articles), using IFT20 antibody in all methods. It is aimed to help Labome visitors find the most suited IFT20 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Proteintech Group
domestic rabbit polyclonal
  • western blot knockout validation; mouse; fig 2
  • immunocytochemistry; mouse; 1:500; fig s5
In order to characterize regulation of craniofacial skeletal development by canonical and noncanonical intraflagellar transport, Proteintech Group IFT20 antibody (Proteintech, 13615-1-AP) was used in western blot knockout validation on mouse samples (fig 2) and in immunocytochemistry on mouse samples at 1:500 (fig s5). Proc Natl Acad Sci U S A (2016) ncbi
Proteintech Group
domestic rabbit polyclonal
  • western blot; human; 1:1500; loading ...; fig 10c
Proteintech Group IFT20 antibody (Proteintech, 13615) was used in western blot on human samples at 1:1500 (fig 10c). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:300; loading ...; fig s6b
  • western blot; human; 1:1000; loading ...; fig s6c
In order to investigate the contribution of PIK3R4 mutations to ciliopathies, Proteintech Group IFT20 antibody (Proteintech, 13615-1-AP) was used in immunocytochemistry on human samples at 1:300 (fig s6b) and in western blot on human samples at 1:1000 (fig s6c). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; fig 2
  • immunocytochemistry; mouse; 1:500; fig s5
In order to characterize regulation of craniofacial skeletal development by canonical and noncanonical intraflagellar transport, Proteintech Group IFT20 antibody (Proteintech, 13615-1-AP) was used in western blot knockout validation on mouse samples (fig 2) and in immunocytochemistry on mouse samples at 1:500 (fig s5). Proc Natl Acad Sci U S A (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 6
  • immunocytochemistry; mouse; 1:200; loading ...; fig 4
MilliporeSigma IFT20 antibody (Sigma, HPA021376) was used in immunocytochemistry on human samples at 1:200 (fig 6) and in immunocytochemistry on mouse samples at 1:200 (fig 4). Cilia (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; dogs; 1:200; loading ...; fig 1c
In order to study primary ciliogenesis in polarized epithelial cells, MilliporeSigma IFT20 antibody (Sigma, HPA021376) was used in immunocytochemistry on dogs samples at 1:200 (fig 1c). J Cell Biol (2016) ncbi
Articles Reviewed
  1. Boukhalfa A, Nascimbeni A, Ramel D, Dupont N, Hirsch E, Gayral S, et al. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nat Commun. 2020;11:294 pubmed publisher
  2. Hua K, Ferland R. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5 pubmed publisher
  3. Stoetzel C, Bär S, De Craene J, Scheidecker S, Etard C, Chicher J, et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun. 2016;7:13586 pubmed publisher
  4. Bernabé Rubio M, Andrés G, Casares Arias J, Fernández Barrera J, Rangel L, Reglero Real N, et al. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells. J Cell Biol. 2016;214:259-73 pubmed publisher
  5. Noda K, Kitami M, Kitami K, Kaku M, Komatsu Y. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Proc Natl Acad Sci U S A. 2016;113:E2589-97 pubmed publisher