This is a Validated Antibody Database (VAD) review about human IFIH1, based on 20 published articles (read how Labome selects the articles), using IFIH1 antibody in all methods. It is aimed to help Labome visitors find the most suited IFIH1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
IFIH1 synonym: AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1; interferon-induced helicase C domain-containing protein 1; CADM-140 autoantigen; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; RIG-I-like receptor 2; RNA helicase-DEAD box protein 116; clinically amyopathic dermatomyositis autoantigen 140 kDa; helicard; helicase with 2 CARD domains; melanoma differentiation-associated gene 5; melanoma differentiation-associated protein 5; murabutide down-regulated protein

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D74E4)
  • western blot knockout validation; mouse; loading ...; fig s8f
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot knockout validation on mouse samples (fig s8f). Hepatology (2018) ncbi
Invitrogen
rabbit monoclonal (33H12L34)
  • immunocytochemistry; human; loading ...; fig 10g
In order to report that rabies virus induces stress granule formation, Invitrogen IFIH1 antibody (Invitrogen, 33H12L34) was used in immunocytochemistry on human samples (fig 10g). PLoS Pathog (2016) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study the relationship between adenosine deaminase acting on RNA 1 (ADAR1) and type I IFNs, Invitrogen IFIH1 antibody (Thermo Scientific, PA5-20337) was used in western blot on mouse samples . J Immunol (2014) ncbi
rabbit monoclonal (33H12L34)
  • western blot; human
In order to study the IFN-lambda inate immune response of HepG2 cells in response to HCV infection, Invitrogen IFIH1 antibody (Invitrogen, 33H12L34) was used in western blot on human samples . Hepatology (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D74E4)
  • western blot; human; 1:3000; loading ...
Cell Signaling Technology IFIH1 antibody (CST, 5321) was used in western blot on human samples at 1:3000. elife (2019) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 3f). PLoS ONE (2018) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; fig s7b
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig s7b). Cell (2018) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; fig 7e
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 7e). Cell (2018) ncbi
rabbit monoclonal (D74E4)
  • western blot knockout validation; mouse; loading ...; fig s8f
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot knockout validation on mouse samples (fig s8f). Hepatology (2018) ncbi
rabbit monoclonal (D74E4)
  • western blot; mouse; 1:2000; loading ...; fig s4f
In order to describe the mechanism by which FBXW7 stabilizes RigI, Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on mouse samples at 1:2000 (fig s4f). Nat Commun (2017) ncbi
rabbit monoclonal (D74E4)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on mouse samples at 1:1000 (fig 4b). Nucleic Acids Res (2017) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; loading ...; fig 7d
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 7d). J Neuroinflammation (2016) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; fig 6
In order to assess the effect of polyinosinic-polycytidylic acid on the barrier function and tight junction integrity of primary human lung microvascular endothelial cells, Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 6). PLoS ONE (2016) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; fig 1
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 1). Sci Rep (2016) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; loading ...; fig 1e
In order to identify the viral RNA signature on RIG-I-like receptors during viral infection, Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 1e). elife (2016) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology IFIH1 antibody (Cell Signaling, D74E4) was used in western blot on human samples at 1:1000 (fig 5). PLoS Pathog (2016) ncbi
rabbit monoclonal (D74E4)
  • western blot; pig; loading ...; fig 10a
Cell Signaling Technology IFIH1 antibody (Cell Signaling, D74E4) was used in western blot on pig samples (fig 10a). MBio (2015) ncbi
rabbit monoclonal (D74E4)
  • western blot; mouse; fig 1
In order to analyze the antiviral innate immune response due to mitochondrial DNA stress, Cell Signaling Technology IFIH1 antibody (Cell Signaling Technology, D74E4) was used in western blot on mouse samples (fig 1). Nature (2015) ncbi
rabbit monoclonal (D74E4)
  • western blot; human; fig 6a
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 6a). Mol Cancer (2014) ncbi
rabbit monoclonal (D74E4)
  • western blot; mouse
Cell Signaling Technology IFIH1 antibody (Cell Signaling, 5321) was used in western blot on mouse samples . PLoS Pathog (2014) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; Chinese tree shrew; fig s3b
In order to investigate the evolution of RIG-I-like receptors, EMD Millipore IFIH1 antibody (Merck Millipore, ABF210) was used in western blot on Chinese tree shrew samples (fig s3b). Proc Natl Acad Sci U S A (2016) ncbi
Articles Reviewed
  1. Xu H, Xu S, Xie S, Zhang Y, Yang J, Zhang W, et al. MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway. elife. 2019;8: pubmed publisher
  2. Ghosh R, Roy S, Franco S. PARP1 depletion induces RIG-I-dependent signaling in human cancer cells. PLoS ONE. 2018;13:e0194611 pubmed publisher
  3. Ahmad S, Mu X, Yang F, Greenwald E, Park J, Jacob E, et al. Breaching Self-Tolerance to Alu Duplex RNA Underlies MDA5-Mediated Inflammation. Cell. 2018;172:797-810.e13 pubmed publisher
  4. Chung H, Calis J, Wu X, Sun T, Yu Y, Sarbanes S, et al. Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. Cell. 2018;172:811-824.e14 pubmed publisher
  5. Wang W, Wang Y, Qu C, Wang S, Zhou J, Cao W, et al. The RNA genome of hepatitis E virus robustly triggers an antiviral interferon response. Hepatology. 2018;67:2096-2112 pubmed publisher
  6. Song Y, Lai L, Chong Z, He J, Zhang Y, Xue Y, et al. E3 ligase FBXW7 is critical for RIG-I stabilization during antiviral responses. Nat Commun. 2017;8:14654 pubmed publisher
  7. Nikolic J, Civas A, Lama Z, Lagaudrière Gesbert C, Blondel D. Rabies Virus Infection Induces the Formation of Stress Granules Closely Connected to the Viral Factories. PLoS Pathog. 2016;12:e1005942 pubmed publisher
  8. Pépin G, Nejad C, Thomas B, Ferrand J, McArthur K, Bardin P, et al. Activation of cGAS-dependent antiviral responses by DNA intercalating agents. Nucleic Acids Res. 2017;45:198-205 pubmed publisher
  9. Xu L, Yu D, Fan Y, Peng L, Wu Y, Yao Y. Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew. Proc Natl Acad Sci U S A. 2016;113:10950-5 pubmed publisher
  10. Zhang X, Zheng Z, Liu X, Shu B, Mao P, Bai B, et al. Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway. J Neuroinflammation. 2016;13:209 pubmed publisher
  11. Huang L, Stuart C, Takeda K, D Agnillo F, Golding B. Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5. PLoS ONE. 2016;11:e0160875 pubmed publisher
  12. Hou Z, Zhang J, Han Q, Su C, Qu J, Xu D, et al. Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a. Sci Rep. 2016;6:26150 pubmed publisher
  13. Sanchez David R, Combredet C, Sismeiro O, Dillies M, Jagla B, Coppée J, et al. Comparative analysis of viral RNA signatures on different RIG-I-like receptors. elife. 2016;5:e11275 pubmed publisher
  14. Pham A, Santa Maria F, Lahiri T, Friedman E, Marié I, Levy D. PKR Transduces MDA5-Dependent Signals for Type I IFN Induction. PLoS Pathog. 2016;12:e1005489 pubmed publisher
  15. Marquez Jurado S, Nogales A, Zuñiga S, Enjuanes L, Almazán F. Identification of a gamma interferon-activated inhibitor of translation-like RNA motif at the 3' end of the transmissible gastroenteritis coronavirus genome modulating innate immune response. MBio. 2015;6:e00105 pubmed publisher
  16. West A, Khoury Hanold W, Staron M, Tal M, Pineda C, Lang S, et al. Mitochondrial DNA stress primes the antiviral innate immune response. Nature. 2015;520:553-7 pubmed publisher
  17. Harashima N, Minami T, Uemura H, Harada M. Transfection of poly(I:C) can induce reactive oxygen species-triggered apoptosis and interferon-β-mediated growth arrest in human renal cell carcinoma cells via innate adjuvant receptors and the 2-5A system. Mol Cancer. 2014;13:217 pubmed publisher
  18. Yang S, Deng P, Zhu Z, Zhu J, Wang G, Zhang L, et al. Adenosine deaminase acting on RNA 1 limits RIG-I RNA detection and suppresses IFN production responding to viral and endogenous RNAs. J Immunol. 2014;193:3436-45 pubmed publisher
  19. Israelow B, Narbus C, Sourisseau M, Evans M. HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection. Hepatology. 2014;60:1170-9 pubmed publisher
  20. Huang Y, Liu H, Li S, Tang Y, Wei B, Yu H, et al. MAVS-MKK7-JNK2 defines a novel apoptotic signaling pathway during viral infection. PLoS Pathog. 2014;10:e1004020 pubmed publisher