This is a Validated Antibody Database (VAD) review about human Hic-5, based on 10 published articles (read how Labome selects the articles), using Hic-5 antibody in all methods. It is aimed to help Labome visitors find the most suited Hic-5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Hic-5 synonym: ARA55; HIC-5; HIC5; TSC-5

Knockout validation
BD Biosciences
mouse monoclonal (34/Hic-5)
  • immunocytochemistry; human; 1:100; loading ...; fig 3f
  • western blot; human; 1:1000; loading ...; fig 3g
  • immunocytochemistry knockout validation; mouse; 1:100; loading ...; fig 3a
BD Biosciences Hic-5 antibody (BD Biosciences, 34) was used in immunocytochemistry on human samples at 1:100 (fig 3f), in western blot on human samples at 1:1000 (fig 3g) and in immunocytochemistry knockout validation on mouse samples at 1:100 (fig 3a). Mol Biol Cell (2019) ncbi
BD Biosciences
mouse monoclonal (34/Hic-5)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1g
  • western blot knockout validation; mouse; loading ...; fig 1i
In order to explore the role of Hic-5 in breast tumor progression, BD Biosciences Hic-5 antibody (BD Biosciences, 611164) was used in immunohistochemistry knockout validation on mouse samples (fig 1g) and in western blot knockout validation on mouse samples (fig 1i). Oncogene (2017) ncbi
BD Biosciences
mouse monoclonal (34/Hic-5)
  • immunocytochemistry; human; loading ...; fig s2j
BD Biosciences Hic-5 antibody (BD Biosciences, 611164) was used in immunocytochemistry on human samples (fig s2j). Cell Rep (2022) ncbi
mouse monoclonal (34/Hic-5)
  • immunocytochemistry; human; 1:100; loading ...; fig 3f
  • western blot; human; 1:1000; loading ...; fig 3g
  • immunocytochemistry knockout validation; mouse; 1:100; loading ...; fig 3a
BD Biosciences Hic-5 antibody (BD Biosciences, 34) was used in immunocytochemistry on human samples at 1:100 (fig 3f), in western blot on human samples at 1:1000 (fig 3g) and in immunocytochemistry knockout validation on mouse samples at 1:100 (fig 3a). Mol Biol Cell (2019) ncbi
mouse monoclonal (34/Hic-5)
  • western blot; human; loading ...; fig 5d
BD Biosciences Hic-5 antibody (BD, 611165) was used in western blot on human samples (fig 5d). J Cell Sci (2017) ncbi
mouse monoclonal (34/Hic-5)
  • western blot; mouse; 1:500; loading ...; fig 1d
BD Biosciences Hic-5 antibody (BD Biosciences, 611164) was used in western blot on mouse samples at 1:500 (fig 1d). Development (2017) ncbi
mouse monoclonal (34/Hic-5)
  • western blot; human; loading ...; fig 2c
In order to describe how Hic-5 regulates glucocorticoid receptor binding site selection, BD Biosciences Hic-5 antibody (BD Transduction Lab, 611165) was used in western blot on human samples (fig 2c). J Biol Chem (2017) ncbi
mouse monoclonal (34/Hic-5)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1g
  • western blot knockout validation; mouse; loading ...; fig 1i
In order to explore the role of Hic-5 in breast tumor progression, BD Biosciences Hic-5 antibody (BD Biosciences, 611164) was used in immunohistochemistry knockout validation on mouse samples (fig 1g) and in western blot knockout validation on mouse samples (fig 1i). Oncogene (2017) ncbi
mouse monoclonal (34/Hic-5)
  • immunocytochemistry; rat; fig 1
In order to study the assembly of integrin adhesome protein complexes, BD Biosciences Hic-5 antibody (BD Transduction, 611164) was used in immunocytochemistry on rat samples (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (34/Hic-5)
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 1
BD Biosciences Hic-5 antibody (BD Transduction Laboratories, 611164) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 1). J Cell Sci (2016) ncbi
mouse monoclonal (34/Hic-5)
  • western blot; human; fig 1
BD Biosciences Hic-5 antibody (BD Biosciences, 611164) was used in western blot on human samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (34/Hic-5)
  • western blot; rat; 1:5000
In order to study the role of TEAD proteins in regulating expression of other genes in vascular smooth muscle cells, BD Biosciences Hic-5 antibody (BD Biosciences, 611164) was used in western blot on rat samples at 1:5000. J Biol Chem (2014) ncbi
Articles Reviewed
  1. Nataraj N, Noronha A, Lee J, Ghosh S, Mohan Raju H, Sekar A, et al. Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors. Cell Rep. 2022;38:110418 pubmed publisher
  2. Vohnoutka R, Gulvady A, Goreczny G, Alpha K, Handelman S, Sexton J, et al. The focal adhesion scaffold protein Hic-5 regulates vimentin organization in fibroblasts. Mol Biol Cell. 2019;30:3037-3056 pubmed publisher
  3. Gao J, Huang M, Lai J, Mao K, Sun P, Cao Z, et al. Kindlin supports platelet integrin αIIbβ3 activation by interacting with paxillin. J Cell Sci. 2017;130:3764-3775 pubmed publisher
  4. Rashid M, Belmont J, Carpenter D, Turner C, Olson E. Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation. Development. 2017;144:4002-4014 pubmed publisher
  5. Lee B, Stallcup M. Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes. J Biol Chem. 2017;292:9320-9334 pubmed publisher
  6. Goreczny G, Ouderkirk Pecone J, Olson E, Krendel M, Turner C. Hic-5 remodeling of the stromal matrix promotes breast tumor progression. Oncogene. 2017;36:2693-2703 pubmed publisher
  7. Harizanova J, Fermin Y, Malik Sheriff R, Wieczorek J, Ickstadt K, Grecco H, et al. Highly Multiplexed Imaging Uncovers Changes in Compositional Noise within Assembling Focal Adhesions. PLoS ONE. 2016;11:e0160591 pubmed publisher
  8. Dave J, Abbey C, Duran C, Seo H, Johnson G, Bayless K. Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase. J Cell Sci. 2016;129:743-56 pubmed publisher
  9. Fujikawa A, Matsumoto M, Kuboyama K, Suzuki R, Noda M. Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5. PLoS ONE. 2015;10:e0119361 pubmed publisher
  10. Liu F, Wang X, Hu G, Wang Y, Zhou J. The transcription factor TEAD1 represses smooth muscle-specific gene expression by abolishing myocardin function. J Biol Chem. 2014;289:3308-16 pubmed publisher