This is a Validated Antibody Database (VAD) review about human HUWE1, based on 8 published articles (read how Labome selects the articles), using HUWE1 antibody in all methods. It is aimed to help Labome visitors find the most suited HUWE1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HUWE1 synonym: ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1; E3 ubiquitin-protein ligase HUWE1; ARF-binding protein 1; BJ-HCC-24 tumor antigen; HECT domain protein LASU1; HECT-type E3 ubiquitin transferase HUWE1; Mcl-1 ubiquitin ligase E3; URE-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9; large structure of UREB1; upstream regulatory element-binding protein 1

Knockout validation
LifeSpan Biosciences
rabbit polyclonal
  • western blot knockout validation; mouse; 1:500; loading ...; fig s2a
In order to investigate the contribution of Huwe1 in proliferating stem cells of the adult mouse hippocampus to the return to quiescence, LifeSpan Biosciences HUWE1 antibody (LifeSpan Biosciences, LS-B1359) was used in western blot knockout validation on mouse samples at 1:500 (fig s2a). Science (2016) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • western blot knockout validation; mouse; 1:500; loading ...; fig s2a
In order to investigate the contribution of Huwe1 in proliferating stem cells of the adult mouse hippocampus to the return to quiescence, LifeSpan Biosciences HUWE1 antibody (LifeSpan Biosciences, LS-B1359) was used in western blot knockout validation on mouse samples at 1:500 (fig s2a). Science (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; rat; fig 4
  • immunocytochemistry; rat; fig 3
  • western blot; rat; 1:700; fig 2
LifeSpan Biosciences HUWE1 antibody (Lifespan, LS-B1359) was used in immunoprecipitation on rat samples (fig 4), in immunocytochemistry on rat samples (fig 3) and in western blot on rat samples at 1:700 (fig 2). Mol Brain (2015) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig st3
  • western blot; rat; 1:1000; loading ...; fig st3
In order to find the E3 ubiquitin ligase Mule is essential for cardiac homeostasis by regulating mitochondrial function via maintenance of Pgc-1alpha and Pink1 expression and persistent negative regulation of c-Myc, Abcam HUWE1 antibody (Abcam, Ab70161) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig st3) and in western blot on rat samples at 1:1000 (fig st3). Sci Rep (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to investigate the role of asymmetric cell division during tumorigenesis, Abcam HUWE1 antibody (Abcam, ab70161) was used in immunocytochemistry on human samples . Cancer Res (2014) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; human; loading ...; fig s2
Novus Biologicals HUWE1 antibody (Novus Biologicals, NB100-652) was used in western blot on human samples (fig s2). Mol Cell Proteomics (2017) ncbi
Cell Signaling Technology
mouse monoclonal (AX8D1)
  • western blot; human; 1:1000; fig s3d
Cell Signaling Technology HUWE1 antibody (Cell Signaling, 5695) was used in western blot on human samples at 1:1000 (fig s3d). Nat Commun (2018) ncbi
ProSci
rabbit polyclonal
  • immunoprecipitation; human
  • western blot; human
ProSci HUWE1 antibody (ProSci, 4213) was used in immunoprecipitation on human samples and in western blot on human samples . Oncogene (2016) ncbi
rabbit polyclonal
  • western blot; human
ProSci HUWE1 antibody (ProSci, 4213) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
Articles Reviewed
  1. Zhang S, Zhang M, Jing Y, Yin X, Ma P, Zhang Z, et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun. 2018;9:215 pubmed publisher
  2. Dadson K, Hauck L, Hao Z, Grothe D, Rao V, Mak T, et al. The E3 ligase Mule protects the heart against oxidative stress and mitochondrial dysfunction through Myc-dependent inactivation of Pgc-1α and Pink1. Sci Rep. 2017;7:41490 pubmed publisher
  3. Crow M, Cristea I. Human Antiviral Protein IFIX Suppresses Viral Gene Expression during Herpes Simplex Virus 1 (HSV-1) Infection and Is Counteracted by Virus-induced Proteasomal Degradation. Mol Cell Proteomics. 2017;16:S200-S214 pubmed publisher
  4. Urbán N, van den Berg D, Forget A, Andersen J, Demmers J, Hunt C, et al. Return to quiescence of mouse neural stem cells by degradation of a proactivation protein. Science. 2016;353:292-5 pubmed publisher
  5. He G, Xu W, Li J, Li S, Liu B, Tan X, et al. Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells. Mol Brain. 2015;8:88 pubmed publisher
  6. Lee C, Yang Y, Chen C, Liu J. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene. 2016;35:1988-95 pubmed publisher
  7. Izumi H, Kaneko Y. Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res. 2014;74:5620-30 pubmed publisher
  8. Khoronenkova S, Dianov G. USP7S-dependent inactivation of Mule regulates DNA damage signalling and repair. Nucleic Acids Res. 2013;41:1750-6 pubmed publisher