This is a Validated Antibody Database (VAD) review about human HSPA4, based on 23 published articles (read how Labome selects the articles), using HSPA4 antibody in all methods. It is aimed to help Labome visitors find the most suited HSPA4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HSPA4 synonym: APG-2; HEL-S-5a; HS24/P52; HSPH2; RY; hsp70; hsp70RY

Knockout validation
multimmune GmbH
Rice black streaked dwarf virus monoclonal
  • immunocytochemistry knockout validation; mouse; fig 2
  • western blot knockout validation; mouse; fig 2
multimmune GmbH HSPA4 antibody (multimmune GmbH, cmHsp70.1) was used in immunocytochemistry knockout validation on mouse samples (fig 2) and in western blot knockout validation on mouse samples (fig 2). PLoS ONE (2014) ncbi
Enzo Life Sciences
domestic rabbit polyclonal
  • western blot; human; 1:5000; loading ...; fig 3c
Enzo Life Sciences HSPA4 antibody (Enzo Life Sciences, ADI-SPA-812-D) was used in western blot on human samples at 1:5000 (fig 3c). Oncogene (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 4
In order to study inhibition of mitochondrial fragmentation and attenuation of kainic acid-induced hippocampal cell death by a mitochondrial division inhibitor, Mdivi-1, Enzo Life Sciences HSPA4 antibody (Enzo, ADI-SPA-812) was used in western blot on mouse samples (fig 4). BMC Neurosci (2016) ncbi
mouse monoclonal (N33F3-4)
  • immunohistochemistry; rat; 1:1000
In order to test if Hsp27 or Hsp70 prevents alpha-synuclein-induced early neuronal pathology in a rat model of Parkinson's disease, Enzo Life Sciences HSPA4 antibody (Stressgen, SPA8135) was used in immunohistochemistry on rat samples at 1:1000. CNS Neurosci Ther (2014) ncbi
System Biosciences
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4c
System Biosciences HSPA4 antibody (System Biosciences, EXOAB-Hsp70A-1) was used in western blot on human samples (fig 4c). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; domestic sheep; 0.25 ug/ml; fig 4
System Biosciences HSPA4 antibody (System Biosciences, EXOAB-HSP70A-1) was used in western blot on domestic sheep samples at 0.25 ug/ml (fig 4). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s1a
In order to examine the role of tissue transglutaminase 2 in the biogenesis of exosomes, System Biosciences HSPA4 antibody (System Biosciences, EXOAB-HSP70A-1) was used in western blot on mouse samples (fig s1a). Biochim Biophys Acta (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1c
Cell Signaling Technology HSPA4 antibody (Cell Signaling, 3303) was used in western blot on human samples (fig 1c). Cells (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1e
Cell Signaling Technology HSPA4 antibody (Cell Signaling, 3303) was used in western blot on human samples (fig 1e). Mol Vis (2017) ncbi
Fitzgerald Industries
mouse monoclonal (4E1)
  • ELISA; human; 1:2500; fig 1e
Fitzgerald Industries HSPA4 antibody (Fitzgerald, 10R-1046) was used in ELISA on human samples at 1:2500 (fig 1e). Arch Pharm Res (2018) ncbi
multimmune GmbH
Rice black streaked dwarf virus monoclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1c
In order to study heat-shock protein 70 in non-small cell lung cancer cells, multimmune GmbH HSPA4 antibody (multimmune GmbH, cmHsp70.1) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1c). Front Immunol (2015) ncbi
Rice black streaked dwarf virus monoclonal
  • immunocytochemistry knockout validation; mouse; fig 2
  • western blot knockout validation; mouse; fig 2
multimmune GmbH HSPA4 antibody (multimmune GmbH, cmHsp70.1) was used in immunocytochemistry knockout validation on mouse samples (fig 2) and in western blot knockout validation on mouse samples (fig 2). PLoS ONE (2014) ncbi
BD Biosciences
mouse monoclonal (7/Hsp70)
  • western blot; human; loading ...; fig 2a
BD Biosciences HSPA4 antibody (BD Biosciences, 610607) was used in western blot on human samples (fig 2a). Sci Adv (2019) ncbi
mouse monoclonal (21/Hsp110)
  • western blot; dogs; 1:500; loading ...; fig 3
BD Biosciences HSPA4 antibody (BD Biosciences, 610511) was used in western blot on dogs samples at 1:500 (fig 3). Int J Hyperthermia (2017) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; loading ...; fig 5d
In order to identify homologous to the E6-AP carboxyl terminus as a ubiquitin ligase for modulator of apoptosis protein 1, BD Biosciences HSPA4 antibody (BD Biosciences, 610607) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 2a
In order to develop a first-in-class humanized antibody against PRL-3 and assess its use as a treatment for gastric cancer, BD Biosciences HSPA4 antibody (BD Pharmingen, 610607) was used in western blot on human samples (fig 2a). JCI Insight (2016) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; mouse
BD Biosciences HSPA4 antibody (BD Transduction, 610608) was used in western blot on mouse samples . Oncoimmunology (2016) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 3
In order to study pVHL-mediated degradation of B-Myb and hypoxia-inducible factor alpha by parallele regulation of von Hippel-Lindau disease, BD Biosciences HSPA4 antibody (BD Transduction Laboratories, 610607) was used in western blot on human samples (fig 3). Mol Cell Biol (2016) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 1b
BD Biosciences HSPA4 antibody (BD Transduction Laboratories, 610607) was used in western blot on human samples (fig 1b). Autophagy (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 1
BD Biosciences HSPA4 antibody (Transduction Laboratories, 610608) was used in western blot on human samples (fig 1). J Extracell Vesicles (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human
BD Biosciences HSPA4 antibody (BD Biosciences, 610607) was used in western blot on human samples . Oncotarget (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; chicken; 1:1000
BD Biosciences HSPA4 antibody (BD Bioscience, 610607) was used in western blot on chicken samples at 1:1000. Biosci Biotechnol Biochem (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; rat
BD Biosciences HSPA4 antibody (Transduction Laboratories, 610607) was used in western blot on rat samples . World J Gastroenterol (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 6
BD Biosciences HSPA4 antibody (BD, 610607) was used in western blot on human samples (fig 6). J Virol (2011) ncbi
Articles Reviewed
  1. Wu P, Hong S, Starenki D, Oshima K, Shao H, Gestwicki J, et al. Mortalin/HSPA9 targeting selectively induces KRAS tumor cell death by perturbing mitochondrial membrane permeability. Oncogene. 2020;39:4257-4270 pubmed publisher
  2. Barbero G, Castro M, Villanueva M, Quezada M, Fernández N, Demorrow S, et al. An Autocrine Wnt5a Loop Promotes NF-κB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma. Cells. 2019;8: pubmed publisher
  3. Sung P, Huang T, Hsieh S. Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019;10:2402 pubmed publisher
  4. May J, Kouri F, Hurley L, Liu J, Tommasini Ghelfi S, Ji Y, et al. IDH3α regulates one-carbon metabolism in glioblastoma. Sci Adv. 2019;5:eaat0456 pubmed publisher
  5. Jin Y, Kim S, Kim H, Ju W, Kim Y, Kim H. Use of protein-based biomarkers of exfoliated cervical cells for primary screening of cervical cancer. Arch Pharm Res. 2018;41:438-449 pubmed publisher
  6. Liu L, Jiang Y, Chahine A, Curtiss E, Steinle J. Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice. Mol Vis. 2017;23:1-7 pubmed
  7. Graner A, Hellwinkel J, Lencioni A, Madsen H, Harland T, Marchando P, et al. HSP90 inhibitors in the context of heat shock and the unfolded protein response: effects on a primary canine pulmonary adenocarcinoma cell line. Int J Hyperthermia. 2017;33:303-317 pubmed publisher
  8. Matsuura K, Huang N, Cocce K, Zhang L, Kornbluth S. Downregulation of the proapoptotic protein MOAP-1 by the UBR5 ubiquitin ligase and its role in ovarian cancer resistance to cisplatin. Oncogene. 2017;36:1698-1706 pubmed publisher
  9. Thura M, Al Aidaroos A, Yong W, Kono K, Gupta A, Lin Y, et al. PRL3-zumab, a first-in-class humanized antibody for cancer therapy. JCI Insight. 2016;1:e87607 pubmed publisher
  10. Finkel P, Frey B, Mayer F, Bösl K, Werthmöller N, Mackensen A, et al. The dual role of NK cells in antitumor reactions triggered by ionizing radiation in combination with hyperthermia. Oncoimmunology. 2016;5:e1101206 pubmed publisher
  11. Deguise M, Boyer J, McFall E, Yazdani A, De Repentigny Y, Kothary R. Differential induction of muscle atrophy pathways in two mouse models of spinal muscular atrophy. Sci Rep. 2016;6:28846 pubmed publisher
  12. Kim H, Lee J, Park K, Kim W, Roh G. A mitochondrial division inhibitor, Mdivi-1, inhibits mitochondrial fragmentation and attenuates kainic acid-induced hippocampal cell death. BMC Neurosci. 2016;17:33 pubmed publisher
  13. Diaz Hidalgo L, Altuntas S, Rossin F, D Eletto M, Marsella C, Farrace M, et al. Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions. Biochim Biophys Acta. 2016;1863:2084-92 pubmed publisher
  14. Okumura F, Uematsu K, Byrne S, Hirano M, Joo Okumura A, Nishikimi A, et al. Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor ?. Mol Cell Biol. 2016;36:1803-17 pubmed publisher
  15. Günther S, Ostheimer C, Stangl S, Specht H, Mózes P, Jesinghaus M, et al. Correlation of Hsp70 Serum Levels with Gross Tumor Volume and Composition of Lymphocyte Subpopulations in Patients with Squamous Cell and Adeno Non-Small Cell Lung Cancer. Front Immunol. 2015;6:556 pubmed publisher
  16. Hieke N, Löffler A, Kaizuka T, Berleth N, Böhler P, Drießen S, et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy. 2015;11:1471-83 pubmed publisher
  17. Lobb R, Becker M, Wen S, Wong C, Wiegmans A, Leimgruber A, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J Extracell Vesicles. 2015;4:27031 pubmed publisher
  18. Pulvino M, Chen L, Oleksyn D, Li J, Compitello G, Rossi R, et al. Inhibition of COP9-signalosome (CSN) deneddylating activity and tumor growth of diffuse large B-cell lymphomas by doxycycline. Oncotarget. 2015;6:14796-813 pubmed
  19. Ueda S, Kokaji Y, Simizu S, Honda K, Yoshino K, Kamisoyama H, et al. Chicken heat shock protein HSPB1 increases and interacts with αB-crystallin in aged skeletal muscle. Biosci Biotechnol Biochem. 2015;79:1867-75 pubmed publisher
  20. Pantazi E, Zaouali M, Bejaoui M, Folch Puy E, Ben Abdennebi H, Varela A, et al. Sirtuin 1 in rat orthotopic liver transplantation: an IGL-1 preservation solution approach. World J Gastroenterol. 2015;21:1765-74 pubmed publisher
  21. Gehrmann M, Stangl S, Foulds G, Oellinger R, Breuninger S, Rad R, et al. Tumor imaging and targeting potential of an Hsp70-derived 14-mer peptide. PLoS ONE. 2014;9:e105344 pubmed publisher
  22. Moloney T, Hyland R, O Toole D, Paucard A, Kirik D, O Doherty A, et al. Heat shock protein 70 reduces ?-synuclein-induced predegenerative neuronal dystrophy in the ?-synuclein viral gene transfer rat model of Parkinson's disease. CNS Neurosci Ther. 2014;20:50-8 pubmed publisher
  23. Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, et al. Hepatitis C virus hijacks P-body and stress granule components around lipid droplets. J Virol. 2011;85:6882-92 pubmed publisher