This is a Validated Antibody Database (VAD) review about human HLTF, based on 6 published articles (read how Labome selects the articles), using HLTF antibody in all methods. It is aimed to help Labome visitors find the most suited HLTF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HLTF synonym: HIP116; HIP116A; HLTF1; RNF80; SMARCA3; SNF2L3; ZBU1; helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor-1 regulator; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SNF2-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3

Abcam
rabbit monoclonal (EPR14761)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 1a
Abcam HLTF antibody (Abcam, ab183042) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 1a). PLoS ONE (2018) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam HLTF antibody (Abcam, ab17984) was used in western blot on mouse samples . PLoS ONE (2013) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig 2
Bethyl HLTF antibody (Bethyl, A300-229A) was used in western blot on human samples (fig 2). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
In order to analyze the divergent interactions with UNG2 and HLTF DNA repair proteins by HIV-1 and HIV-2, Bethyl HLTF antibody (Bethyl Laboratories, A300-230A) was used in western blot on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl HLTF antibody (Bethyl, A300-230A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to study the involvement of TIMELESS in the PARP1-mediated DNA damage response, Bethyl HLTF antibody (Bethyl, A300-229A) was used in western blot on human samples (fig 4). Cell Rep (2015) ncbi
Articles Reviewed
  1. Kaur G, Helmer R, Smith L, Martinez Zaguilan R, Dufour J, Chilton B. Alternative splicing of helicase-like transcription factor (Hltf): Intron retention-dependent activation of immune tolerance at the feto-maternal interface. PLoS ONE. 2018;13:e0200211 pubmed publisher
  2. Hampp S, Kiessling T, Buechle K, Mansilla S, Thomale J, Rall M, et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A. 2016;113:E4311-9 pubmed publisher
  3. Hrecka K, Hao C, Shun M, Kaur S, Swanson S, Florens L, et al. HIV-1 and HIV-2 exhibit divergent interactions with HLTF and UNG2 DNA repair proteins. Proc Natl Acad Sci U S A. 2016;113:E3921-30 pubmed publisher
  4. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  5. Young L, Marzio A, Pérez Durán P, Reid D, Meredith D, Roberti D, et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep. 2015;13:451-459 pubmed publisher
  6. Helmer R, Martinez Zaguilan R, Dertien J, Fulford C, Foreman O, Peiris V, et al. Helicase-like transcription factor (Hltf) regulates G2/M transition, Wt1/Gata4/Hif-1a cardiac transcription networks, and collagen biogenesis. PLoS ONE. 2013;8:e80461 pubmed publisher