This is a Validated Antibody Database (VAD) review about human HLA-C, based on 36 published articles (read how Labome selects the articles), using HLA-C antibody in all methods. It is aimed to help Labome visitors find the most suited HLA-C antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HLA-C synonym: D6S204; HLA-JY3; HLAC; HLC-C; MHC; PSORS1; HLA class I histocompatibility antigen, Cw-1 alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA-C alpha chain; HLA-C antigen; MHC class I antigen heavy chain HLA-C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C

Invitrogen
mouse monoclonal (W6/32)
  • flow cytometry; human; loading ...; fig 1a
Invitrogen HLA-C antibody (eBioscience, W6/32) was used in flow cytometry on human samples (fig 1a). J Immunol (2018) ncbi
mouse monoclonal (W6/32)
  • blocking or activating experiments; human; loading ...; fig s2b
Invitrogen HLA-C antibody (eBiosciences, w6/32) was used in blocking or activating experiments on human samples (fig s2b). Nat Med (2018) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; loading ...; fig 1a
In order to evaluate the effectiveness of adoptive natural killer cell therapy against the pulmonary metastasis of Ewing sarcoma, Invitrogen HLA-C antibody (eBiosciences, W6/32) was used in flow cytometry on human samples (fig 1a). Oncoimmunology (2017) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; loading ...; fig 1a
In order to develop a recombinant Saccharomyces cerevisiae as a vehicle to deliver genes to primary human macrophages, Invitrogen HLA-C antibody (eBioscience, W6/32) was used in flow cytometry on human samples (fig 1a). Eur J Pharm Biopharm (2017) ncbi
mouse monoclonal (W6/32)
  • immunohistochemistry; human; 1:5000; loading ...
In order to assess the diagnostic value of myxovirus resistance A expression for dermatomyositis, Invitrogen HLA-C antibody (Thermo Fisher, W6/32) was used in immunohistochemistry on human samples at 1:5000. Neurology (2017) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; loading ...; fig s9a
Invitrogen HLA-C antibody (eBiosciences, W6/32) was used in flow cytometry on human samples (fig s9a). PLoS Pathog (2016) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; fig 1
In order to analyze how Saccharomyces cerevisiae interacts with different macrophage subsets, Invitrogen HLA-C antibody (eBioscience, W6/32) was used in flow cytometry on human samples (fig 1). Inflammation (2016) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; loading ...; fig s9a
In order to discuss how signal peptidase complex proteins affect flaviviruses, Invitrogen HLA-C antibody (eBiosciences, W6/32) was used in flow cytometry on human samples (fig s9a). Nature (2016) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; 1:50; fig s2j
In order to develop and characterize a humanized ossicle xenotransplantation approach, Invitrogen HLA-C antibody (ebioscience, W6/32) was used in flow cytometry on human samples at 1:50 (fig s2j). Nat Med (2016) ncbi
mouse monoclonal (W6/32)
  • immunohistochemistry - paraffin section; human; 1:5000; fig 4
In order to determine if there is an association between inclusion body myositis and hepatitis C virus infection, Invitrogen HLA-C antibody (Thermo Fisher Scientific, W6/32) was used in immunohistochemistry - paraffin section on human samples at 1:5000 (fig 4). Neurology (2016) ncbi
mouse monoclonal (W6/32)
  • immunoprecipitation; human
  • western blot; human
In order to study lactacystin and IFN-gamma in human salivary glands, Invitrogen HLA-C antibody (Thermo Fisher, MA1-19027) was used in immunoprecipitation on human samples and in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (W6/32)
  • western blot; human
In order to report that the human herpesvirus-7 U21 gene product interferes with natural killer cell recognition, Invitrogen HLA-C antibody (Zymed Laboratories, W6/32) was used in western blot on human samples . PLoS Pathog (2011) ncbi
Abcam
rabbit monoclonal (EPR6749)
  • immunohistochemistry; human; loading ...; fig 2b
  • western blot; human; 1:1000; loading ...; fig 2a
Abcam HLA-C antibody (AbCam, ab126722) was used in immunohistochemistry on human samples (fig 2b) and in western blot on human samples at 1:1000 (fig 2a). Biol Reprod (2018) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; 1:6000; loading ...; fig s7b
In order to investigate the effectiveness of a neoantigen vaccine against melanoma, Abcam HLA-C antibody (Abcam, EMR8-5) was used in immunohistochemistry - paraffin section on human samples at 1:6000 (fig s7b). Nature (2017) ncbi
mouse monoclonal (EMR8-5)
  • western blot; human; 1:2000; loading ...; fig 6a
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Abcam HLA-C antibody (Abcam, ab70328) was used in western blot on human samples at 1:2000 (fig 6a). Nat Commun (2017) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; loading ...; tbl 2
In order to characterize HLA class I-positive and negative tumors, Abcam HLA-C antibody (Abcam, ab70328) was used in immunohistochemistry - paraffin section on human samples (tbl 2). Int J Cancer (2017) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; fig 6
Abcam HLA-C antibody (Abcam, ab70328) was used in immunohistochemistry - paraffin section on human samples (fig 6). Neoplasia (2016) ncbi
mouse monoclonal (EMR8-5)
  • western blot; human; fig 6a
In order to study the results of proteomic screening that identified calreticulin as a miR-27a direct target that represses MHC clas 1 cell surface exposure in colorectal cancer, Abcam HLA-C antibody (Abcam, ab70328) was used in western blot on human samples (fig 6a). Cell Death Dis (2016) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; 1:350; fig 4
In order to elucidate a screening that identifies kinases involved in prostate cancer visceral and bone metastasis, Abcam HLA-C antibody (Abcam, ab70328) was used in immunohistochemistry - paraffin section on human samples at 1:350 (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
In order to analyze a model for ductal pancreatic cancer and drug screening, Abcam HLA-C antibody (Abcam, ab70328) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3). Nat Med (2015) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; fig 3
Abcam HLA-C antibody (Abcam, ab70328) was used in immunohistochemistry - paraffin section on human samples (fig 3). Br J Cancer (2015) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry - paraffin section; human; fig 4
Abcam HLA-C antibody (Abcam, ab70328) was used in immunohistochemistry - paraffin section on human samples (fig 4). Stem Cells Int (2015) ncbi
mouse monoclonal (EMR8-5)
  • immunohistochemistry; human; 1:500; fig 5
In order to identify alterations in genes involved in antigen presentation, chromosome integrity, transcriptional regulation, and ubiquitination in Hodgkin and Reed-Sternberg cells, Abcam HLA-C antibody (Abcam, EMR8-5) was used in immunohistochemistry on human samples at 1:500 (fig 5). Blood (2015) ncbi
mouse monoclonal (EMR8-5)
  • immunocytochemistry; human
Abcam HLA-C antibody (Abcam, ab70328) was used in immunocytochemistry on human samples . Stem Cell Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (LY5.1)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology HLA-C antibody (Santa Cruz Biotechnology, sc-52810) was used in western blot on human samples (fig 3a). Oncoimmunology (2018) ncbi
mouse monoclonal (LY5.1)
  • western blot; human; 1:500; fig 2
Santa Cruz Biotechnology HLA-C antibody (Santa Cruz Technologies, SC-52810) was used in western blot on human samples at 1:500 (fig 2). Cancer Res (2015) ncbi
mouse monoclonal (W6/32)
  • western blot; human
Santa Cruz Biotechnology HLA-C antibody (Santa Cruz, sc-32235) was used in western blot on human samples . J Virol (2015) ncbi
Epigentek
goat polyclonal
  • western blot; human; fig 2
Epigentek HLA-C antibody (Epigentek, A4030) was used in western blot on human samples (fig 2). Nucleic Acids Res (2016) ncbi
Beckman Coulter
mouse monoclonal (B9.12.1)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, Beckman Coulter HLA-C antibody (Beckman Coulter (Immunotech), B9.12.1) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (B9.12.1)
  • flow cytometry; human; fig 2
Beckman Coulter HLA-C antibody (Beckman, B9.12.1) was used in flow cytometry on human samples (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (B9.12.1)
  • flow cytometry; human
Beckman Coulter HLA-C antibody (Beckman Coulter, B9.12.1) was used in flow cytometry on human samples . Transpl Immunol (2015) ncbi
Sigma-Aldrich
mouse monoclonal (W6/32)
  • flow cytometry; human; fig 1a
In order to elucidate the effects of pathogen recognition receptors on dendritic cell maturation, HIV infection, and on the quality of HIV-specific cytotoxic T-cell activation, Sigma-Aldrich HLA-C antibody (Sigma-Aldrich, W6/32) was used in flow cytometry on human samples (fig 1a). Eur J Immunol (2017) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; fig 1
Sigma-Aldrich HLA-C antibody (Sigma-Aldrich, F5662) was used in flow cytometry on human samples (fig 1). Stem Cell Reports (2016) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human
  • immunoprecipitation; human
In order to study how Kaposi's sarcoma-associated herpes virus K3 viral gene product decreases cell surface MHC class I, Sigma-Aldrich HLA-C antibody (Sigma-Aldrich, w6/32) was used in flow cytometry on human samples and in immunoprecipitation on human samples . Biochem J (2015) ncbi
mouse monoclonal (W6/32)
  • flow cytometry; human; fig 5
Sigma-Aldrich HLA-C antibody (Sigma, H1650) was used in flow cytometry on human samples (fig 5). J Virol (2014) ncbi
EMD Millipore
mouse monoclonal (DT9)
  • flow cytometry; human; fig 2c
In order to analyze HIV-1 Vpu sequences isolated from patients, EMD Millipore HLA-C antibody (Millipore, MABF233) was used in flow cytometry on human samples (fig 2c). Sci Rep (2017) ncbi
Articles Reviewed
  1. Walwyn Brown K, Guldevall K, Saeed M, Pende D, Önfelt B, MacDonald A, et al. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1. J Immunol. 2018;201:2028-2041 pubmed publisher
  2. Luo N, Formisano L, Gonzalez Ericsson P, Sanchez V, Dean P, Opalenik S, et al. Melanoma response to anti-PD-L1 immunotherapy requires JAK1 signaling, but not JAK2. Oncoimmunology. 2018;7:e1438106 pubmed publisher
  3. Zacharakis N, Chinnasamy H, Black M, Xu H, Lu Y, Zheng Z, et al. Immune recognition of somatic mutations leading to complete durable regression in metastatic breast cancer. Nat Med. 2018;24:724-730 pubmed publisher
  4. Warthan M, Washington S, Franzese S, Ramus R, Kim K, York T, et al. The role of endoplasmic reticulum aminopeptidase 2 in modulating immune detection of choriocarcinoma. Biol Reprod. 2018;98:309-322 pubmed publisher
  5. Ott P, Hu Z, Keskin D, Shukla S, Sun J, Bozym D, et al. An immunogenic personal neoantigen vaccine for patients with melanoma. Nature. 2017;547:217-221 pubmed publisher
  6. Tong A, Hashem H, Eid S, Allen F, Kingsley D, Huang A. Adoptive natural killer cell therapy is effective in reducing pulmonary metastasis of Ewing sarcoma. Oncoimmunology. 2017;6:e1303586 pubmed publisher
  7. Yamashita Y, Anczurowski M, Nakatsugawa M, Tanaka M, Kagoya Y, Sinha A, et al. HLA-DP84Gly constitutively presents endogenous peptides generated by the class I antigen processing pathway. Nat Commun. 2017;8:15244 pubmed publisher
  8. Seif M, Hoppstädter J, Breinig F, Kiemer A. Yeast-mediated mRNA delivery polarizes immuno-suppressive macrophages towards an immuno-stimulatory phenotype. Eur J Pharm Biopharm. 2017;117:1-13 pubmed publisher
  9. Romani B, Kavyanifard A, Allahbakhshi E. Functional conservation and coherence of HIV-1 subtype A Vpu alleles. Sci Rep. 2017;7:44894 pubmed publisher
  10. Cardinaud S, Urrutia A, Rouers A, Coulon P, Kervevan J, Richetta C, et al. Triggering of TLR-3, -4, NOD2, and DC-SIGN reduces viral replication and increases T-cell activation capacity of HIV-infected human dendritic cells. Eur J Immunol. 2017;47:818-829 pubmed publisher
  11. Uruha A, Nishikawa A, Tsuburaya R, Hamanaka K, Kuwana M, Watanabe Y, et al. Sarcoplasmic MxA expression: A valuable marker of dermatomyositis. Neurology. 2017;88:493-500 pubmed publisher
  12. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  13. Tomic A, Varanasi P, Golemac M, Malic S, Riese P, Borst E, et al. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand. PLoS Pathog. 2016;12:e1006015 pubmed publisher
  14. Perea F, Bernal M, Sánchez Palencia A, Carretero J, Torres C, Bayarri C, et al. The absence of HLA class I expression in non-small cell lung cancer correlates with the tumor tissue structure and the pattern of T cell infiltration. Int J Cancer. 2017;140:888-899 pubmed publisher
  15. Sugita S, Iwasaki Y, Makabe K, Kimura T, Futagami T, Suegami S, et al. Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. Stem Cell Reports. 2016;7:619-634 pubmed publisher
  16. Huang M, Zhang W, Guo J, Wei X, Phiwpan K, Zhang J, et al. Improved Transgenic Mouse Model for Studying HLA Class I Antigen Presentation. Sci Rep. 2016;6:33612 pubmed publisher
  17. Jung I, Chung Y, Jung D, Kim Y, Kim D, Kim K, et al. Impaired Lymphocytes Development and Xenotransplantation of Gastrointestinal Tumor Cells in Prkdc-Null SCID Zebrafish Model. Neoplasia. 2016;18:468-79 pubmed publisher
  18. Seif M, Philippi A, Breinig F, Kiemer A, Hoppstädter J. Yeast (Saccharomyces cerevisiae) Polarizes Both M-CSF- and GM-CSF-Differentiated Macrophages Toward an M1-Like Phenotype. Inflammation. 2016;39:1690-703 pubmed publisher
  19. Zhang R, Miner J, Gorman M, Rausch K, Ramage H, White J, et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature. 2016;535:164-8 pubmed
  20. Reinisch A, Thomas D, Corces M, Zhang X, Gratzinger D, Hong W, et al. A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells. Nat Med. 2016;22:812-21 pubmed publisher
  21. Onyango D, Howard S, Neherin K, Yanez D, Stark J. Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res. 2016;44:5702-16 pubmed publisher
  22. Colangelo T, Polcaro G, Ziccardi P, Pucci B, Muccillo L, Galgani M, et al. Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer. Cell Death Dis. 2016;7:e2120 pubmed publisher
  23. Uruha A, Noguchi S, Hayashi Y, Tsuburaya R, Yonekawa T, Nonaka I, et al. Hepatitis C virus infection in inclusion body myositis: A case-control study. Neurology. 2016;86:211-7 pubmed publisher
  24. Faltermeier C, Drake J, Clark P, Smith B, Zong Y, Volpe C, et al. Functional screen identifies kinases driving prostate cancer visceral and bone metastasis. Proc Natl Acad Sci U S A. 2016;113:E172-81 pubmed publisher
  25. Huang L, Holtzinger A, Jagan I, BeGora M, Lohse I, Ngai N, et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med. 2015;21:1364-71 pubmed publisher
  26. Thuring C, Follin E, Geironson L, Freyhult E, Junghans V, Harndahl M, et al. HLA class I is most tightly linked to levels of tapasin compared with other antigen-processing proteins in glioblastoma. Br J Cancer. 2015;113:952-62 pubmed publisher
  27. Vishnubalaji R, Atteya M, Al Nbaheen M, Oreffo R, Aldahmash A, Alajez N. Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model. Stem Cells Int. 2015;2015:257019 pubmed publisher
  28. Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed publisher
  29. Miranda A, Funes J, Sánchez N, Limia C, Mesa M, Quezada S, et al. Oncogenic Transformation Can Orchestrate Immune Evasion and Inflammation in Human Mesenchymal Stem Cells Independently of Extrinsic Immune-Selective Pressure. Cancer Res. 2015;75:3032-42 pubmed publisher
  30. Guo X, Liu T, Shi H, Wang J, Ji P, Wang H, et al. Respiratory Syncytial Virus Infection Upregulates NLRC5 and Major Histocompatibility Complex Class I Expression through RIG-I Induction in Airway Epithelial Cells. J Virol. 2015;89:7636-45 pubmed publisher
  31. Esquivel E, Maeda A, Eguchi H, Asada M, Sugiyama M, Manabe C, et al. Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G. Transpl Immunol. 2015;32:109-15 pubmed publisher
  32. Reichel J, Chadburn A, Rubinstein P, Giulino Roth L, Tam W, Liu Y, et al. Flow sorting and exome sequencing reveal the oncogenome of primary Hodgkin and Reed-Sternberg cells. Blood. 2015;125:1061-72 pubmed publisher
  33. Gabaev I, Elbasani E, Ameres S, Steinbrück L, Stanton R, Döring M, et al. Expression of the human cytomegalovirus UL11 glycoprotein in viral infection and evaluation of its effect on virus-specific CD8 T cells. J Virol. 2014;88:14326-39 pubmed publisher
  34. Arellano Garcia M, Misuno K, Tran S, Hu S. Interferon-? induces immunoproteasomes and the presentation of MHC I-associated peptides on human salivary gland cells. PLoS ONE. 2014;9:e102878 pubmed publisher
  35. Mannino M, Gomez Roman N, Hochegger H, Chalmers A. Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors: implications for stem cell mitosis and centrosome dynamics. Stem Cell Res. 2014;13:135-43 pubmed publisher
  36. Schneider C, Hudson A. The human herpesvirus-7 (HHV-7) U21 immunoevasin subverts NK-mediated cytoxicity through modulation of MICA and MICB. PLoS Pathog. 2011;7:e1002362 pubmed publisher