This is a Validated Antibody Database (VAD) review about human HK1, based on 26 published articles (read how Labome selects the articles), using HK1 antibody in all methods. It is aimed to help Labome visitors find the most suited HK1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HK1 synonym: HK; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; NEDVIBA; RP79; hexokinase

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (C35C4)
  • western blot knockout validation; human; 1:2000; loading ...; fig 4d
Cell Signaling Technology HK1 antibody (CST, 2024) was used in western blot knockout validation on human samples at 1:2000 (fig 4d). Nature (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-1)
  • western blot; mouse; 1:1000; loading ...; fig 4e
Santa Cruz Biotechnology HK1 antibody (SantaCruz, sc-46695) was used in western blot on mouse samples at 1:1000 (fig 4e). Cell Signal (2018) ncbi
mouse monoclonal (G-1)
  • western blot; mouse; 1:500; fig 3c
Santa Cruz Biotechnology HK1 antibody (SantaCruz, G-1) was used in western blot on mouse samples at 1:500 (fig 3c). Int J Biochem Cell Biol (2017) ncbi
mouse monoclonal (4D7)
  • western blot; human; fig 2
Santa Cruz Biotechnology HK1 antibody (Santa Cruz, sc-80978) was used in western blot on human samples (fig 2). Pflugers Arch (2016) ncbi
Invitrogen
mouse monoclonal (7A7)
  • western blot; human; 1:500; loading ...; fig 6d
Invitrogen HK1 antibody (Thermo Fisher Scientific, MA5-15675) was used in western blot on human samples at 1:500 (fig 6d). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (R.457.3)
  • western blot; human; loading ...; fig 1
In order to elucidate the role of hexokinases in colorectal cancer growth, Invitrogen HK1 antibody (Thermo Fisher, MA5-14789) was used in western blot on human samples (fig 1). BMC Genet (2016) ncbi
MilliporeSigma
mouse monoclonal (5G9)
  • immunohistochemistry - frozen section; rat; 1:100; loading ...; fig 2a
  • immunoprecipitation; rat; loading ...; fig 1b
  • western blot; rat; 1:500; loading ...; fig 1a
MilliporeSigma HK1 antibody (Sigma, WH0003098M1) was used in immunohistochemistry - frozen section on rat samples at 1:100 (fig 2a), in immunoprecipitation on rat samples (fig 1b) and in western blot on rat samples at 1:500 (fig 1a). J Neurosci Res (2015) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig 7a
Abcam HK1 antibody (ABcam, ab65069) was used in western blot on human samples at 1:2000 (fig 7a). J Exp Clin Cancer Res (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C35C4)
  • western blot knockout validation; human; 1:2000; loading ...; fig 4d
Cell Signaling Technology HK1 antibody (CST, 2024) was used in western blot knockout validation on human samples at 1:2000 (fig 4d). Nature (2019) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; 1:1000; loading ...; fig 4b, s6, s18b
Cell Signaling Technology HK1 antibody (Cell Signaling, C35C4) was used in western blot on human samples at 1:1000 (fig 4b, s6, s18b). Nat Commun (2019) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; loading ...; fig s2p
Cell Signaling Technology HK1 antibody (CST, 2024) was used in western blot on mouse samples (fig s2p). Cell (2019) ncbi
domestic rabbit monoclonal (C35C4)
Cell Signaling Technology HK1 antibody (Cell Signaling Technology, 2024) was used . Cell Rep (2019) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; fig 4a
Cell Signaling Technology HK1 antibody (Cell signaling, 2024) was used in western blot on mouse samples (fig 4a). Immunity (2017) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; fig 2g
Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on human samples (fig 2g). Nature (2017) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; fig 5h
Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on human samples (fig 5h). Sci Rep (2017) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; loading ...; fig af6e
Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on mouse samples (fig af6e). Mol Syst Biol (2017) ncbi
domestic rabbit monoclonal (C35C4)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2d
Cell Signaling Technology HK1 antibody (Cell Signaling, mAb2024) was used in immunohistochemistry on mouse samples at 1:100 (fig 2d). Nat Commun (2016) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; loading ...; fig 3g
In order to test if LKB1 mutations contribute to cervical cancer initiation and progress, Cell Signaling Technology HK1 antibody (Cell signaling, 2024) was used in western blot on mouse samples (fig 3g). Oncogene (2017) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; 1:1000; loading ...; fig s1
In order to evaluate the effects of pioglitazone on human Sertoli cells metabolism, Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on human samples at 1:1000 (fig s1). Int J Biochem Cell Biol (2016) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; fig 3
Cell Signaling Technology HK1 antibody (Cell Signaling, C35C4) was used in western blot on human samples (fig 3). Sci Rep (2016) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; 1:1000; loading ...; fig 5e
Cell Signaling Technology HK1 antibody (Cell Signaling, cs-2024) was used in western blot on mouse samples at 1:1000 (fig 5e). EMBO Mol Med (2016) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; 1:1000; fig 4b
Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on human samples at 1:1000 (fig 4b). Cell Death Dis (2015) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; human; loading ...; fig 3c
In order to quantify proteins involved in mitochondrial oxidation and glucose and lipid metabolism in skeletal muscle of trained and untrained middle-aged men, Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on human samples (fig 3c). Physiol Res (2016) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; 1:1000; fig 2
In order to use of chaperone-mediated autophagy to facilitate lipolysis by degradation of lipid droplet-associated proteins, Cell Signaling Technology HK1 antibody (Cell signaling, C35C4) was used in western blot on mouse samples at 1:1000 (fig 2). Nat Cell Biol (2015) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; loading ...; fig 2a
Cell Signaling Technology HK1 antibody (Cell Signaling, C35C4) was used in western blot on mouse samples (fig 2a). Hum Mol Genet (2015) ncbi
domestic rabbit monoclonal (C35C4)
  • western blot; mouse; 1:1000
In order to determine the role of Gsk3a in male fertility using KO mice, Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in western blot on mouse samples at 1:1000. Biol Reprod (2015) ncbi
domestic rabbit monoclonal (C35C4)
  • immunohistochemistry - paraffin section; mouse; 1:800; fig s11
In order to assess effects nuclear factor-erythroid 2-related factor 1 deficiency in beta-cells on beta-cell function and glucose homeostasis, Cell Signaling Technology HK1 antibody (Cell Signaling, 2024) was used in immunohistochemistry - paraffin section on mouse samples at 1:800 (fig s11). Antioxid Redox Signal (2015) ncbi
Articles Reviewed
  1. Amendola C, Mahaffey J, Parker S, Ahearn I, Chen W, Zhou M, et al. KRAS4A directly regulates hexokinase 1. Nature. 2019;576:482-486 pubmed publisher
  2. Ghezzi C, Wong A, Chen B, Ribalet B, Damoiseaux R, Clark P. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nat Commun. 2019;10:5444 pubmed publisher
  3. Mogilenko D, Haas J, L homme L, Fleury S, Quemener S, Levavasseur M, et al. Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR. Cell. 2019;177:1201-1216.e19 pubmed publisher
  4. Zhang Q, Higginbotham J, Jeppesen D, Yang Y, Li W, McKinley E, et al. Transfer of Functional Cargo in Exomeres. Cell Rep. 2019;27:940-954.e6 pubmed publisher
  5. Peng S, Gerasimenko J, Tsugorka T, Gryshchenko O, Samarasinghe S, Petersen O, et al. Galactose protects against cell damage in mouse models of acute pancreatitis. J Clin Invest. 2018;128:3769-3778 pubmed publisher
  6. Schönrogge M, Kerndl H, Zhang X, Kumstel S, Vollmar B, Zechner D. α-cyano-4-hydroxycinnamate impairs pancreatic cancer cells by stimulating the p38 signaling pathway. Cell Signal. 2018;47:101-108 pubmed publisher
  7. Kishore M, Cheung K, Fu H, Bonacina F, Wang G, Coe D, et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis. Immunity. 2017;47:875-889.e10 pubmed publisher
  8. Yu L, Sun Y, Li J, Wang Y, Zhu Y, Shi Y, et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res. 2017;36:110 pubmed publisher
  9. Yu P, Wilhelm K, Dubrac A, Tung J, Alves T, Fang J, et al. FGF-dependent metabolic control of vascular development. Nature. 2017;545:224-228 pubmed publisher
  10. Zhao X, Sun K, Lan Z, Song W, Cheng L, Chi W, et al. Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity. Sci Rep. 2017;7:46344 pubmed publisher
  11. André F, Trinh A, Balayssac S, Maboudou P, Dekiouk S, Malet Martino M, et al. Metabolic rewiring in cancer cells overexpressing the glucocorticoid-induced leucine zipper protein (GILZ): Activation of mitochondrial oxidative phosphorylation and sensitization to oxidative cell death induced by mitochondrial targeted drugs. Int J Biochem Cell Biol. 2017;85:166-174 pubmed publisher
  12. Graham N, Minasyan A, Lomova A, Cass A, Balanis N, Friedman M, et al. Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. Mol Syst Biol. 2017;13:914 pubmed publisher
  13. Kudryavtseva A, Fedorova M, Zhavoronkov A, Moskalev A, Zasedatelev A, Dmitriev A, et al. Effect of lentivirus-mediated shRNA inactivation of HK1, HK2, and HK3 genes in colorectal cancer and melanoma cells. BMC Genet. 2016;17:156 pubmed publisher
  14. Hinckelmann M, Virlogeux A, Niehage C, Poujol C, Choquet D, Hoflack B, et al. Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport. Nat Commun. 2016;7:13233 pubmed publisher
  15. Zeng Q, Chen J, Li Y, Werle K, Zhao R, Quan C, et al. LKB1 inhibits HPV-associated cancer progression by targeting cellular metabolism. Oncogene. 2017;36:1245-1255 pubmed publisher
  16. Meneses M, Bernardino R, Sa R, Silva J, Barros A, Sousa M, et al. Pioglitazone increases the glycolytic efficiency of human Sertoli cells with possible implications for spermatogenesis. Int J Biochem Cell Biol. 2016;79:52-60 pubmed publisher
  17. Broniarek I, Koziel A, Jarmuszkiewicz W. The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells. Pflugers Arch. 2016;468:1541-54 pubmed publisher
  18. Ma T, Fan B, Zhang C, Zhao H, Han C, Gao C, et al. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Sci Rep. 2016;6:29926 pubmed publisher
  19. Albert V, Svensson K, Shimobayashi M, Colombi M, Munoz S, Jimenez V, et al. mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue. EMBO Mol Med. 2016;8:232-46 pubmed publisher
  20. Carpentieri A, Cozzoli E, Scimeca M, Bonanno E, Sardanelli A, Gambacurta A. Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor. Cell Death Dis. 2015;6:e1974 pubmed publisher
  21. Vigelso A, Prats C, Ploug T, Dela F, Helge J. Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men. Physiol Res. 2016;65:293-302 pubmed
  22. Kaushik S, Cuervo A. Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis. Nat Cell Biol. 2015;17:759-70 pubmed publisher
  23. Corsetti V, Florenzano F, Atlante A, Bobba A, Ciotti M, Natale F, et al. NH2-truncated human tau induces deregulated mitophagy in neurons by aberrant recruitment of Parkin and UCHL-1: implications in Alzheimer's disease. Hum Mol Genet. 2015;24:3058-81 pubmed publisher
  24. Bhattacharjee R, Goswami S, Dudiki T, Popkie A, Phiel C, Kline D, et al. Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility. Biol Reprod. 2015;92:65 pubmed publisher
  25. Zheng H, Fu J, Xue P, Zhao R, Dong J, Liu D, et al. CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion. Antioxid Redox Signal. 2015;22:819-31 pubmed publisher
  26. Jackson J, O Donnell J, Krizman E, Robinson M. Displacing hexokinase from mitochondrial voltage-dependent anion channel impairs GLT-1-mediated glutamate uptake but does not disrupt interactions between GLT-1 and mitochondrial proteins. J Neurosci Res. 2015;93:999-1008 pubmed publisher