This is a Validated Antibody Database (VAD) review about human HGS, based on 8 published articles (read how Labome selects the articles), using HGS antibody in all methods. It is aimed to help Labome visitors find the most suited HGS antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HGS synonym: HRS; hepatocyte growth factor-regulated tyrosine kinase substrate; human growth factor-regulated tyrosine kinase substrate; protein pp110

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D7T5N)
  • western blot knockout validation; human; 1:1000; loading ...; fig 2b
Cell Signaling Technology HGS antibody (Cell Signaling, 15087) was used in western blot knockout validation on human samples at 1:1000 (fig 2b). Cell Rep (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-3)
  • western blot; human; 1:1000; loading ...; fig s2a
Santa Cruz Biotechnology HGS antibody (Santa Cruz, sc-271925) was used in western blot on human samples at 1:1000 (fig s2a). Nat Commun (2017) ncbi
mouse monoclonal (C-7)
  • immunocytochemistry; human; loading ...; fig 8c
  • western blot; human; loading ...; fig 8b
Santa Cruz Biotechnology HGS antibody (Santa Cruz Biotechnology, Sc-271455) was used in immunocytochemistry on human samples (fig 8c) and in western blot on human samples (fig 8b). PLoS Pathog (2016) ncbi
mouse monoclonal (D-6)
  • immunohistochemistry - frozen section; mouse; 1:200
  • immunocytochemistry; mouse; 1:200
In order to study STAM2 in the nervous system, Santa Cruz Biotechnology HGS antibody (Santa Cruz, sc166843) was used in immunohistochemistry - frozen section on mouse samples at 1:200 and in immunocytochemistry on mouse samples at 1:200. Mol Cell Neurosci (2015) ncbi
Abcam
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4a
  • western blot; mouse; 1:1000; loading ...; fig 4b
In order to elucidate a novel pathway for mitochondrial elimination, in which these organelles undergo Parkin-dependent sequestration into Rab5-positive early endosomes via the ESCRT machinery, Abcam HGS antibody (Abcam, ab155539) was used in immunocytochemistry on mouse samples at 1:100 (fig 4a) and in western blot on mouse samples at 1:1000 (fig 4b). Nat Commun (2017) ncbi
Cell Signaling Technology
rabbit monoclonal (D7T5N)
  • western blot knockout validation; human; 1:1000; loading ...; fig 2b
Cell Signaling Technology HGS antibody (Cell Signaling, 15087) was used in western blot knockout validation on human samples at 1:1000 (fig 2b). Cell Rep (2017) ncbi
rabbit monoclonal (D7T5N)
  • western blot; human; loading ...; fig s7e
In order to establish an integrated multilayered proteomics approach to study receptor signaling, Cell Signaling Technology HGS antibody (Cell Signaling, 15087) was used in western blot on human samples (fig s7e). Nat Struct Mol Biol (2016) ncbi
Sigma-Aldrich
mouse monoclonal (6D11)
  • western blot; human; 1:500; fig 3
Sigma-Aldrich HGS antibody (Sigma, WH0009146) was used in western blot on human samples at 1:500 (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (6D11)
  • immunocytochemistry; human; 1:500; fig 5
  • western blot; human; 1:1000; fig 2
Sigma-Aldrich HGS antibody (Sigma, WH0009146M1) was used in immunocytochemistry on human samples at 1:500 (fig 5) and in western blot on human samples at 1:1000 (fig 2). elife (2016) ncbi
Articles Reviewed
  1. Goodwin J, Dowdle W, Dejesus R, Wang Z, Bergman P, Kobylarz M, et al. Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9. Cell Rep. 2017;20:2341-2356 pubmed publisher
  2. Bednash J, Weathington N, Londino J, Rojas M, Gulick D, Fort R, et al. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Nat Commun. 2017;8:15203 pubmed publisher
  3. Hammerling B, Najor R, Cortez M, Shires S, Leon L, Gonzalez E, et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun. 2017;8:14050 pubmed publisher
  4. Kumar B, Dutta D, Iqbal J, Ansari M, Roy A, Chikoti L, et al. ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016;12:e1005960 pubmed publisher
  5. Sun Y, Zheng W, Guo Z, Ju Q, Zhu L, Gao J, et al. A novel TP53 pathway influences the HGS-mediated exosome formation in colorectal cancer. Sci Rep. 2016;6:28083 pubmed publisher
  6. Francavilla C, Papetti M, Rigbolt K, Pedersen A, Sigurdsson J, Cazzamali G, et al. Multilayered proteomics reveals molecular switches dictating ligand-dependent EGFR trafficking. Nat Struct Mol Biol. 2016;23:608-18 pubmed publisher
  7. Gschweitl M, Ulbricht A, Barnes C, Enchev R, Stoffel Studer I, Meyer Schaller N, et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. elife. 2016;5:e13841 pubmed publisher
  8. Kapuralin K, Ćurlin M, Mitrečić D, Kosi N, Schwarzer C, Glavan G, et al. STAM2, a member of the endosome-associated complex ESCRT-0 is highly expressed in neurons. Mol Cell Neurosci. 2015;67:104-15 pubmed publisher