This is a Validated Antibody Database (VAD) review about human HCK, based on 4 published articles (read how Labome selects the articles), using HCK antibody in all methods. It is aimed to help Labome visitors find the most suited HCK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HCK synonym: JTK9; p59Hck; p61Hck; tyrosine-protein kinase HCK; hematopoietic cell kinase; hemopoietic cell kinase; p59-HCK/p60-HCK

Abcam
rabbit polyclonal
  • western blot; human; loading ...; fig 1d
In order to characterize a new specific pharmacological hematopoietic cell kinase inhibitor, Abcam HCK antibody (Abcam, ab61055) was used in western blot on human samples (fig 1d). Biochim Biophys Acta Mol Basis Dis (2017) ncbi
rabbit monoclonal (EP2415Y)
  • western blot; human; loading ...; fig 1d
In order to characterize a new specific pharmacological hematopoietic cell kinase inhibitor, Abcam HCK antibody (Abcam, ab75839) was used in western blot on human samples (fig 1d). Biochim Biophys Acta Mol Basis Dis (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3D12E10)
  • immunoprecipitation; mouse; fig 9
  • western blot; mouse; fig 7
In order to determine the response threshold of immunoreceptor tyrosine-based activation motifs, Santa Cruz Biotechnology HCK antibody (Santa Cruz, 1428) was used in immunoprecipitation on mouse samples (fig 9) and in western blot on mouse samples (fig 7). elife (2015) ncbi
BD Biosciences
mouse monoclonal (18/Hck)
  • other; human; loading ...; fig st1
  • western blot; human; loading ...; fig st4
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences HCK antibody (BD, 18) was used in other on human samples (fig st1) and in western blot on human samples (fig st4). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (18/Hck)
  • western blot; cow; 1:1000; fig 3b
In order to elucidate the acquired motility and invasiveness of T. annulata-infected cells, BD Biosciences HCK antibody (BD, 610278) was used in western blot on cow samples at 1:1000 (fig 3b). PLoS Pathog (2014) ncbi
Articles Reviewed
  1. Roversi F, Pericole F, Machado Neto J, da Silva Santos Duarte A, Longhini A, Corrocher F, et al. Hematopoietic cell kinase (HCK) is a potential therapeutic target for dysplastic and leukemic cells due to integration of erythropoietin/PI3K pathway and regulation of erythropoiesis: HCK in erythropoietin/PI3K pathway. Biochim Biophys Acta Mol Basis Dis. 2017;1863:450-461 pubmed publisher
  2. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  3. Freedman T, Tan Y, Skrzypczynska K, Manz B, Sjaastad F, Goodridge H, et al. LynA regulates an inflammation-sensitive signaling checkpoint in macrophages. elife. 2015;4: pubmed publisher
  4. Ma M, Baumgartner M. Intracellular Theileria annulata promote invasive cell motility through kinase regulation of the host actin cytoskeleton. PLoS Pathog. 2014;10:e1004003 pubmed publisher