This is a Validated Antibody Database (VAD) review about human HBG1, based on 19 published articles (read how Labome selects the articles), using HBG1 antibody in all methods. It is aimed to help Labome visitors find the most suited HBG1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HBG1 synonym: HBG-T2; HBGA; HBGR; HSGGL1; PRO2979; hemoglobin subunit gamma-1; A-gamma globin; gamma A hemoglobin; gamma globin; gamma-1-globin; hb F Agamma; hemoglobin gamma-1 chain; hemoglobin gamma-a chain; hemoglobin, gamma A; hemoglobin, gamma, regulator of

Invitrogen
mouse monoclonal (HBF-1)
  • flow cytometry; mouse; loading ...; fig 2c
Invitrogen HBG1 antibody (Invitrogen, HBF-1) was used in flow cytometry on mouse samples (fig 2c). Sci Rep (2018) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; fig 2a
Invitrogen HBG1 antibody (Invitrogen, MHFH05) was used in flow cytometry on human samples (fig 2a). Science (2018) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; loading ...; fig st1
Invitrogen HBG1 antibody (Life Technologies, HbF-1) was used in flow cytometry on human samples (fig st1). Nat Genet (2017) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; fig 2
In order to demonstrate that chemical inhibition of HDAC1/2 induces gamma-globin and propose a role for the GATA2 gene in this process, Invitrogen HBG1 antibody (Life Technologies, HbF-1) was used in flow cytometry on human samples (fig 2). PLoS ONE (2016) ncbi
mouse monoclonal (HBF-1)
  • immunocytochemistry; human; fig 6
In order to show that forced chromatin looping can override a stringent developmental gene expression program, Invitrogen HBG1 antibody (Invitrogen, MHFH05) was used in immunocytochemistry on human samples (fig 6). Cell (2014) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; fig 2
In order to develop a method using primary human bone marrow-derived erythroid progenitor cells to screen for compounds that induce gamma-globin production, Invitrogen HBG1 antibody (Life Technologies, MHFH01) was used in flow cytometry on human samples (fig 2). J Biomol Screen (2013) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human
In order to describe a method to generate red blood cells ex vivo using human erythroid precursors, Invitrogen HBG1 antibody (Invitrogen, MHFH01) was used in flow cytometry on human samples . Mol Ther (2014) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; 5 ul; fig 1
In order to report that LSD1 is a promising therapeutic target for gamma-globin induction, Invitrogen HBG1 antibody (Invitrogen, clone MHFH05) was used in flow cytometry on human samples at 5 ul (fig 1). Nat Med (2013) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; fig 3
In order to assess flow cytometric protocols for the quantification feto-maternal hemorrhage, Invitrogen HBG1 antibody (Zymed, MHFH04) was used in flow cytometry on human samples (fig 3). Transfus Med (2003) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human; fig 3
In order to describe a new method to detect fetal hemoglobin, Invitrogen HBG1 antibody (Caltag, MHFH05) was used in flow cytometry on human samples (fig 3). Cytometry (2000) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human
  • immunocytochemistry; human; fig 3
In order to assess the feasibility of using the orange autofluorescence signal to detect fetal hemoglobin, Invitrogen HBG1 antibody (Caltag, noca) was used in flow cytometry on human samples and in immunocytochemistry on human samples (fig 3). Cytometry (2000) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human
In order to develop immunostaining methods to measure intracellular hemoglobin in erythrocytes by fluorescence activated cell flow cytometry, Invitrogen HBG1 antibody (noco, noca) was used in flow cytometry on human samples . Br J Haematol (1994) ncbi
mouse monoclonal (HBF-1)
  • flow cytometry; human
In order to develop a flow cytometry-based method to measure fetal-maternal hemorrhage, Invitrogen HBG1 antibody (noco, noca) was used in flow cytometry on human samples . Am J Clin Pathol (1989) ncbi
Santa Cruz Biotechnology
mouse monoclonal (51-7)
  • western blot; human; 1:1000; fig 2c
Santa Cruz Biotechnology HBG1 antibody (Santa, sc-21756) was used in western blot on human samples at 1:1000 (fig 2c). Science (2018) ncbi
mouse monoclonal (51-7)
  • western blot; human; 1:2000; loading ...; fig 2a
Santa Cruz Biotechnology HBG1 antibody (Santa Cruz Biotechnology, SC-21756) was used in western blot on human samples at 1:2000 (fig 2a). Nat Commun (2017) ncbi
mouse monoclonal (51-7)
  • flow cytometry; human; loading ...; fig s3d
  • western blot; human; fig s7c
In order to elucidate the mechanisms by which fetal-type hemoglobin is repressed, Santa Cruz Biotechnology HBG1 antibody (Santa Cruz, sc-21756) was used in flow cytometry on human samples (fig s3d) and in western blot on human samples (fig s7c). Science (2016) ncbi
mouse monoclonal (51-7)
  • western blot; human; fig 3
Santa Cruz Biotechnology HBG1 antibody (Santa Cruz, sc-21756) was used in western blot on human samples (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (51-7)
  • western blot; human
Santa Cruz Biotechnology HBG1 antibody (Santa Cruz Biotechnology, sc-21756) was used in western blot on human samples . Mol Biol Cell (2014) ncbi
mouse monoclonal (51-7)
  • western blot; human
Santa Cruz Biotechnology HBG1 antibody (Santa Cruz Biotechnology, sc-21756) was used in western blot on human samples . Mol Cell Biochem (2014) ncbi
mouse monoclonal (51-7)
  • immunocytochemistry; human; 1:75
  • western blot; human; 1:100
Santa Cruz Biotechnology HBG1 antibody (Santa Cruz, SC-21756) was used in immunocytochemistry on human samples at 1:75 and in western blot on human samples at 1:100. Int J Oncol (2014) ncbi
Articles Reviewed
  1. Yen J, Fiorino M, Liu Y, Paula S, Clarkson S, Quinn L, et al. TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells. Sci Rep. 2018;8:16304 pubmed publisher
  2. Grevet J, Lan X, Hamagami N, Edwards C, Sankaranarayanan L, Ji X, et al. Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells. Science. 2018;361:285-290 pubmed publisher
  3. Trakarnsanga K, Griffiths R, Wilson M, Blair A, Satchwell T, Meinders M, et al. An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells. Nat Commun. 2017;8:14750 pubmed publisher
  4. Canver M, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern E, et al. Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017;49:625-634 pubmed publisher
  5. Shearstone J, Golonzhka O, Chonkar A, Tamang D, Van Duzer J, Jones S, et al. Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS ONE. 2016;11:e0153767 pubmed publisher
  6. Masuda T, Wang X, Maeda M, Canver M, Sher F, Funnell A, et al. Transcription factors LRF and BCL11A independently repress expression of fetal hemoglobin. Science. 2016;351:285-9 pubmed publisher
  7. Boosalis M, Sangerman J, White G, Wolf R, Shen L, Dai Y, et al. Novel Inducers of Fetal Globin Identified through High Throughput Screening (HTS) Are Active In Vivo in Anemic Baboons and Transgenic Mice. PLoS ONE. 2015;10:e0144660 pubmed publisher
  8. Deng W, Rupon J, Krivega I, Breda L, Motta I, Jahn K, et al. Reactivation of developmentally silenced globin genes by forced chromatin looping. Cell. 2014;158:849-860 pubmed publisher
  9. Miyazawa M, Tsuji Y. Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene. Mol Biol Cell. 2014;25:2116-27 pubmed publisher
  10. Peretti de Albuquerque Wobeto V, Machado Neto J, Zaccariotto T, Ribeiro D, da Silva Santos Duarte A, Saad S, et al. PIPKII? is widely expressed in hematopoietic-derived cells and may play a role in the expression of alpha- and gamma-globins in K562 cells. Mol Cell Biochem. 2014;393:145-53 pubmed publisher
  11. Emara M, Turner A, Allalunis Turner J. Adult, embryonic and fetal hemoglobin are expressed in human glioblastoma cells. Int J Oncol. 2014;44:514-20 pubmed publisher
  12. Li H, Xie W, Gore E, Montoute M, Bee W, Zappacosta F, et al. Development of phenotypic screening assays for ?-globin induction using primary human bone marrow day 7 erythroid progenitor cells. J Biomol Screen. 2013;18:1212-22 pubmed publisher
  13. Huang X, Shah S, Wang J, Ye Z, Dowey S, Tsang K, et al. Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors. Mol Ther. 2014;22:451-463 pubmed publisher
  14. Shi L, Cui S, Engel J, Tanabe O. Lysine-specific demethylase 1 is a therapeutic target for fetal hemoglobin induction. Nat Med. 2013;19:291-4 pubmed publisher
  15. Kennedy G, Shaw R, Just S, Bryson G, Battistutta F, Rowell J, et al. Quantification of feto-maternal haemorrhage (FMH) by flow cytometry: anti-fetal haemoglobin labelling potentially underestimates massive FMH in comparison to labelling with anti-D. Transfus Med. 2003;13:25-33 pubmed
  16. Mundee Y, Bigelow N, Davis B, Porter J. Simplified flow cytometric method for fetal hemoglobin containing red blood cells. Cytometry. 2000;42:389-93 pubmed
  17. Chen J, Bigelow N, Davis B. Proposed flow cytometric reference method for the determination of erythroid F-cell counts. Cytometry. 2000;42:239-46 pubmed
  18. Thorpe S, Thein S, Sampietro M, Craig J, Mahon B, Huehns E. Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis. Br J Haematol. 1994;87:125-32 pubmed
  19. Nance S, Nelson J, Arndt P, Lam H, Garratty G. Quantitation of fetal-maternal hemorrhage by flow cytometry. A simple and accurate method. Am J Clin Pathol. 1989;91:288-92 pubmed