This is a Validated Antibody Database (VAD) review about human H4C4, based on 34 published articles (read how Labome selects the articles), using H4C4 antibody in all methods. It is aimed to help Labome visitors find the most suited H4C4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
H4C4 synonym: H4-16; H4/b; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C5; H4C6; H4C8; H4C9; H4FB; HIST1H4D; dJ221C16.9

Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 2a
  • western blot; mouse; 1:1000; fig 2c
Abcam H4C4 antibody (Abcam, ab5823) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 2a) and in western blot on mouse samples at 1:1000 (fig 2c). Int J Biol Sci (2022) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 7e
  • western blot; mouse; 1:1000; loading ...; fig 6a
Abcam H4C4 antibody (Abcam, ab5823) was used in chromatin immunoprecipitation on mouse samples (fig 7e) and in western blot on mouse samples at 1:1000 (fig 6a). Front Cell Dev Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1d
  • flow cytometry; human; loading ...; fig 2e
Abcam H4C4 antibody (Abcam, ab7311) was used in western blot on mouse samples (fig 1d) and in flow cytometry on human samples (fig 2e). J Clin Invest (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2b
Abcam H4C4 antibody (Abcam, ab9052) was used in western blot on human samples at 1:1000 (fig 2b). Nature (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse
Abcam H4C4 antibody (Abcam, ab9052) was used in immunocytochemistry on mouse samples . Nature (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 4b
Abcam H4C4 antibody (Abcam, ab46983) was used in western blot on human samples at 1:500 (fig 4b). Cell Death Dis (2019) ncbi
mouse monoclonal (mAbcam 31830)
  • immunocytochemistry; human; loading ...; fig 6d
Abcam H4C4 antibody (Abcam, ab197515) was used in immunocytochemistry on human samples (fig 6d). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; brewer's yeast; 1:2000; loading ...; fig 3c
Abcam H4C4 antibody (Abcam, ab46983) was used in western blot on brewer's yeast samples at 1:2000 (fig 3c). J Vis Exp (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 5a
Abcam H4C4 antibody (Abcam, ab5823) was used in western blot on human samples at 1:1000 (fig 5a). Cell Death Dis (2019) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6a
Abcam H4C4 antibody (abcam, ab7311) was used in immunoprecipitation on human samples (fig 6a). Mol Cell Biol (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 7c
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Abcam H4C4 antibody (Abcam, AB7311) was used in western blot on human samples (fig 7c). Mol Cell (2017) ncbi
domestic rabbit polyclonal
  • other; human; 1:200; loading ...; fig 3
Abcam H4C4 antibody (Abcam, ab9052) was used in other on human samples at 1:200 (fig 3). Nat Chem Biol (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 2b
  • western blot; human; loading ...; fig 5c
In order to determine the molecular mechanisms that enable JAK2/signal transducer and activator of transcription 5 to access promoters of genes implicated in breast cancer pathogenesis, Abcam H4C4 antibody (Abcam, ab7311) was used in chromatin immunoprecipitation on human samples (fig 2b) and in western blot on human samples (fig 5c). J Biol Chem (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
Abcam H4C4 antibody (Abcam, AB7311) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4b
In order to propose that acrolein alters chromatin assembly by reacting with lysine residues of histones, Abcam H4C4 antibody (Abcam, ab61238) was used in western blot on human samples (fig 4b). Mol Cell Biol (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; loading ...; fig 2c
Abcam H4C4 antibody (Abcam, ab5823) was used in chromatin immunoprecipitation on human samples (fig 2c) and in western blot on human samples (fig 2c). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; brewer's yeast; fig 4C
In order to elucidate the interaction between Eaf3/5/7, NuA4, and RNA polymerase II, Abcam H4C4 antibody (Abcam, ab7311) was used in western blot on brewer's yeast samples (fig 4C). J Biol Chem (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig st3
In order to study the contribution of SFMBT2 to prostate cancer metastasis, Abcam H4C4 antibody (Abcam, ab9052) was used in chromatin immunoprecipitation on human samples (fig st3). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; Entamoeba histolytica; 1:300; fig 3
  • western blot; Entamoeba histolytica; 1:2000
  • western blot; bovine; 1:2000
Abcam H4C4 antibody (Abcam, ab61238) was used in immunocytochemistry on Entamoeba histolytica samples at 1:300 (fig 3), in western blot on Entamoeba histolytica samples at 1:2000 and in western blot on bovine samples at 1:2000. Parasit Vectors (2016) ncbi
Invitrogen
domestic rabbit monoclonal (E.960.2)
  • western blot; human; fig 6
In order to test if celastrol inhibits formation of neutrophil extracellular traps induced by inflammatory stimuli associated with rheumatoid arthritis and systemic lupus erythematosus, Invitrogen H4C4 antibody (Thermo Fisher Scientific, MA5-15150) was used in western blot on human samples (fig 6). Curr Mol Med (2015) ncbi
Active Motif
domestic rabbit polyclonal
  • western blot; human; 1:5000; loading ...; fig 6a
Active Motif H4C4 antibody (Active Motif, 39167) was used in western blot on human samples at 1:5000 (fig 6a). elife (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D12B3)
  • western blot; mouse; loading ...; fig 2b
Cell Signaling Technology H4C4 antibody (Cell Signaling, 8647) was used in western blot on mouse samples (fig 2b). Cells (2020) ncbi
domestic rabbit monoclonal (D84D2)
  • western blot; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology H4C4 antibody (Cell signaling, D84D2) was used in western blot on human samples at 1:1000 (fig 3b). Aging (Albany NY) (2019) ncbi
mouse monoclonal (L64C1)
  • western blot; mouse; loading ...; fig s1d
Cell Signaling Technology H4C4 antibody (Cell Signalling, L64C1) was used in western blot on mouse samples (fig s1d). Nucleic Acids Res (2019) ncbi
mouse monoclonal (L64C1)
  • western blot; mouse; loading ...; fig 3e
Cell Signaling Technology H4C4 antibody (Cell Signaling, 2935) was used in western blot on mouse samples (fig 3e). Cell (2019) ncbi
mouse monoclonal (L64C1)
  • western blot; human; loading ...; fig 1c
In order to study the role of ACK1/TNK2 in histone H4 Tyr88-phosphorylation and androgen receptor expression in prostate cancer, Cell Signaling Technology H4C4 antibody (Cell Signaling, 2935) was used in western blot on human samples (fig 1c). Cancer Cell (2017) ncbi
domestic rabbit monoclonal (D12B3)
  • chromatin immunoprecipitation; rat; loading ...; fig 7c
In order to research the role of HDAC9 in regulating kidney angiotensinogen expression, Cell Signaling Technology H4C4 antibody (Cell Signaling, 8647) was used in chromatin immunoprecipitation on rat samples (fig 7c). Biol Sex Differ (2017) ncbi
mouse monoclonal (L64C1)
  • western blot; mouse; loading ...; fig 5
Cell Signaling Technology H4C4 antibody (cell signalling, L64C1) was used in western blot on mouse samples (fig 5). J Cell Sci (2017) ncbi
domestic rabbit polyclonal
  • other; human; 1:100; loading ...; fig 3
Cell Signaling Technology H4C4 antibody (Cell Signal, 9724) was used in other on human samples at 1:100 (fig 3). Nat Chem Biol (2017) ncbi
domestic rabbit polyclonal
  • other; human; 1:1000; loading ...; fig s9
Cell Signaling Technology H4C4 antibody (Cell Signal, 2594) was used in other on human samples at 1:1000 (fig s9). Nat Chem Biol (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...
In order to investigate the role of FACT in sensing DNA torsional stress, Cell Signaling Technology H4C4 antibody (Cell Signaling, 2592P) was used in western blot on human samples . Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1d
In order to investigate the mechanism by which vorinostat suppresses matrix metallopeptidase-13, Cell Signaling Technology H4C4 antibody (Cell Signaling, 9672) was used in western blot on human samples at 1:1000 (fig 1d). Am J Pathol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1d
In order to investigate the mechanism by which vorinostat suppresses matrix metallopeptidase-13, Cell Signaling Technology H4C4 antibody (Cell Signaling, 2592) was used in western blot on human samples at 1:1000 (fig 1d). Am J Pathol (2016) ncbi
domestic rabbit monoclonal (D84D2)
  • immunohistochemistry - paraffin section; human; fig 1
Cell Signaling Technology H4C4 antibody (Cell Signaling, cs5737) was used in immunohistochemistry - paraffin section on human samples (fig 1). Genome Biol (2016) ncbi
mouse monoclonal (L64C1)
  • western blot; human; loading ...; fig 2b
In order to show that FLCN regulates mTORC1 by modulating the leucine signal in lysosome, Cell Signaling Technology H4C4 antibody (Cell Signalling, 2935) was used in western blot on human samples (fig 2b). PLoS ONE (2016) ncbi
mouse monoclonal (L64C1)
  • western blot; rat
Cell Signaling Technology H4C4 antibody (Cell Signaling, 2935) was used in western blot on rat samples . PLoS ONE (2015) ncbi
mouse monoclonal (L64C1)
  • western blot; human
Cell Signaling Technology H4C4 antibody (Cell Signaling, 2935) was used in western blot on human samples . Eur Respir J (2014) ncbi
Articles Reviewed
  1. Tang Y, Dong L, Zhang C, Li X, Li R, Lin H, et al. PRMT5 acts as a tumor suppressor by inhibiting Wnt/β-catenin signaling in murine gastric tumorigenesis. Int J Biol Sci. 2022;18:4329-4340 pubmed publisher
  2. Dong F, Chen M, Jiang L, Shen Z, Ma L, Han C, et al. PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Plzf Expression via Modulation of Lysine Histone Modifications. Front Cell Dev Biol. 2021;9:673258 pubmed publisher
  3. Kim C, Jin J, Ye Z, Jadhav R, Gustafson C, Hu B, et al. Histone deficiency and accelerated replication stress in T cell aging. J Clin Invest. 2021;131: pubmed publisher
  4. Yuan G, Flores N, Hausmann S, Lofgren S, Kharchenko V, Angulo Ibáñez M, et al. Elevated NSD3 histone methylation activity drives squamous cell lung cancer. Nature. 2021;590:504-508 pubmed publisher
  5. Takei Y, Yun J, Zheng S, Ollikainen N, Pierson N, White J, et al. Integrated spatial genomics reveals global architecture of single nuclei. Nature. 2021;590:344-350 pubmed publisher
  6. Alam S, Piazzesi A, Abd El Fatah M, Raucamp M, van Echten Deckert G. Neurodegeneration Caused by S1P-Lyase Deficiency Involves Calcium-Dependent Tau Pathology and Abnormal Histone Acetylation. Cells. 2020;9: pubmed publisher
  7. Wang Z, Millard C, Lin C, Gurnett J, Wu M, Lee K, et al. Diverse nucleosome site-selectivity among histone deacetylase complexes. elife. 2020;9: pubmed publisher
  8. Perri A, Agosti V, Olivo E, Concolino A, Angelis M, Tammè L, et al. Histone proteomics reveals novel post-translational modifications in breast cancer. Aging (Albany NY). 2019;11:11722-11755 pubmed publisher
  9. Turberfield A, Kondo T, Nakayama M, Koseki Y, King H, Koseki H, et al. KDM2 proteins constrain transcription from CpG island gene promoters independently of their histone demethylase activity. Nucleic Acids Res. 2019;47:9005-9023 pubmed publisher
  10. Shan C, Lu Z, Li Z, Sheng H, Fan J, Qi Q, et al. 4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis. Cell Death Dis. 2019;10:525 pubmed publisher
  11. Alim I, Caulfield J, Chen Y, Swarup V, Geschwind D, Ivanova E, et al. Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke. Cell. 2019;177:1262-1279.e25 pubmed publisher
  12. Koupenova M, Corkrey H, Vitseva O, Manni G, Pang C, Clancy L, et al. The role of platelets in mediating a response to human influenza infection. Nat Commun. 2019;10:1780 pubmed publisher
  13. Bennett S, Cobos S, Meykler M, Fallah M, Rana N, Chen K, et al. Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models. J Vis Exp. 2019;: pubmed publisher
  14. Demetriadou C, Pavlou D, Mpekris F, Achilleos C, Stylianopoulos T, Zaravinos A, et al. NAA40 contributes to colorectal cancer growth by controlling PRMT5 expression. Cell Death Dis. 2019;10:236 pubmed publisher
  15. Fujimoto M, Takii R, Katiyar A, Srivastava P, Nakai A. Poly(ADP-Ribose) Polymerase 1 Promotes the Human Heat Shock Response by Facilitating Heat Shock Transcription Factor 1 Binding to DNA. Mol Cell Biol. 2018;38: pubmed publisher
  16. Mahajan K, Malla P, Lawrence H, Chen Z, Kumar Sinha C, Malik R, et al. ACK1/TNK2 Regulates Histone H4 Tyr88-phosphorylation and AR Gene Expression in Castration-Resistant Prostate Cancer. Cancer Cell. 2017;31:790-803.e8 pubmed publisher
  17. Bourgeois C, Satou R, Prieto M. HDAC9 is an epigenetic repressor of kidney angiotensinogen establishing a sex difference. Biol Sex Differ. 2017;8:18 pubmed publisher
  18. Jha K, Tripurani S, Johnson G. TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis. J Cell Sci. 2017;130:1835-1844 pubmed publisher
  19. Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  20. He Y, Selvaraju S, Curtin M, Jakob C, Zhu H, Comess K, et al. The EED protein-protein interaction inhibitor A-395 inactivates the PRC2 complex. Nat Chem Biol. 2017;13:389-395 pubmed publisher
  21. Safina A, Cheney P, Pal M, Brodsky L, Ivanov A, Kirsanov K, et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res. 2017;45:1925-1945 pubmed publisher
  22. Schauwecker S, Kim J, Licht J, Clevenger C. Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells. J Biol Chem. 2017;292:2237-2254 pubmed publisher
  23. Cooper S, Grijzenhout A, Underwood E, Ancelin K, Zhang T, Nesterova T, et al. Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2. Nat Commun. 2016;7:13661 pubmed publisher
  24. Fang L, Chen D, Yu C, Li H, Brocato J, Huang L, et al. Mechanisms Underlying Acrolein-Mediated Inhibition of Chromatin Assembly. Mol Cell Biol. 2016;36:2995-3008 pubmed publisher
  25. Makki M, Haqqi T. Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1?-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte. Am J Pathol. 2016;186:2701-8 pubmed publisher
  26. Deng X, Shao G, Zhang H, Li C, Zhang D, Cheng L, et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene. 2017;36:1223-1231 pubmed publisher
  27. Sathianathan A, Ravichandran P, Lippi J, Cohen L, Messina A, Shaju S, et al. The Eaf3/5/7 Subcomplex Stimulates NuA4 Interaction with Methylated Histone H3 Lys-36 and RNA Polymerase II. J Biol Chem. 2016;291:21195-21207 pubmed
  28. Nelson D, Jaber Hijazi F, Cole J, Robertson N, Pawlikowski J, Norris K, et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol. 2016;17:158 pubmed publisher
  29. Gwak J, Shin J, Lee K, Hong S, Oh S, Goh S, et al. SFMBT2 (Scm-like with four mbt domains 2) negatively regulates cell migration and invasion in prostate cancer cells. Oncotarget. 2016;7:48250-48264 pubmed publisher
  30. Wu X, Zhao L, Chen Z, Ji X, Qiao X, Jin Y, et al. FLCN Maintains the Leucine Level in Lysosome to Stimulate mTORC1. PLoS ONE. 2016;11:e0157100 pubmed publisher
  31. Lozano Amado D, Herrera Solorio A, Valdés J, Alemán Lazarini L, Almaraz Barrera M, Luna Rivera E, et al. Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica. Parasit Vectors. 2016;9:19 pubmed publisher
  32. Yu Y, Koehn C, Yue Y, Li S, Thiele G, Hearth Holmes M, et al. Celastrol inhibits inflammatory stimuli-induced neutrophil extracellular trap formation. Curr Mol Med. 2015;15:401-10 pubmed
  33. Kim B, Serebreni L, Fallica J, Hamdan O, Wang L, Johnston L, et al. Cyclin-dependent kinase five mediates activation of lung xanthine oxidoreductase in response to hypoxia. PLoS ONE. 2015;10:e0124189 pubmed publisher
  34. Sanders Y, Hagood J, Liu H, Zhang W, Ambalavanan N, Thannickal V. Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice. Eur Respir J. 2014;43:1448-58 pubmed publisher