This is a Validated Antibody Database (VAD) review about human H3-4, based on 17 published articles (read how Labome selects the articles), using H3-4 antibody in all methods. It is aimed to help Labome visitors find the most suited H3-4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
H3-4 synonym: H3.4; H3/g; H3FT; H3t; HIST3H3

Abcam
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; 1:1000; loading ...; fig 2d
Abcam H3-4 antibody (Abcam, ab2621) was used in chromatin immunoprecipitation on mouse samples at 1:1000 (fig 2d). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2g
Abcam H3-4 antibody (Abcam, ab2621) was used in western blot on human samples (fig 2g). Theranostics (2020) ncbi
mouse monoclonal (mAbcam12209)
  • ChIP-Seq; human; ; loading ...; fig 3s3a
Abcam H3-4 antibody (Abcam, ab12209) was used in ChIP-Seq on human samples at (fig 3s3a). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Abcam H3-4 antibody (Abcam, ab2621) was used in western blot on human samples (fig 2a). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 6b
In order to assess the effect of TNF-alpha inhibitors on monocyte chemoattractant protein-1, Abcam H3-4 antibody (Millipore, ab2621) was used in chromatin immunoprecipitation on human samples (fig 6b). Mol Immunol (2017) ncbi
mouse monoclonal (mAbcam12209)
  • immunohistochemistry; mouse; 1:200; loading ...; fig s6w
In order to demonstrate that some mitochondrial enzymes associated with the tricarboxylic acid cycle are essential for epigenetic remodeling and transiently localize to the nucleus, Abcam H3-4 antibody (Abcam, ab12209) was used in immunohistochemistry on mouse samples at 1:200 (fig s6w). Cell (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; loading ...; fig 2c
Abcam H3-4 antibody (Abcam, ab130740) was used in chromatin immunoprecipitation on human samples (fig 2c) and in western blot on human samples (fig 2c). Oncogene (2017) ncbi
mouse monoclonal (mAbcam12209)
  • chromatin immunoprecipitation; human; loading ...; fig 3c
In order to elucidate how p21 is suppressed in embryonic stem cells, Abcam H3-4 antibody (Abcam, ab12209) was used in chromatin immunoprecipitation on human samples (fig 3c). Sci Rep (2016) ncbi
mouse monoclonal (mAbcam12209)
  • immunocytochemistry; human
Abcam H3-4 antibody (Abcam, ab12209) was used in immunocytochemistry on human samples . Hum Genet (2015) ncbi
mouse monoclonal (mAbcam12209)
  • blocking or activating experiments; mouse; 1:200
  • immunocytochemistry; mouse
Abcam H3-4 antibody (Abcam, ab12209) was used in blocking or activating experiments on mouse samples at 1:200 and in immunocytochemistry on mouse samples . Cell Death Dis (2014) ncbi
mouse monoclonal (mAbcam12209)
  • immunohistochemistry; mouse; loading ...; fig 8a
  • western blot; mouse; loading ...; fig 7a
Abcam H3-4 antibody (Abcam, ab12209) was used in immunohistochemistry on mouse samples (fig 8a) and in western blot on mouse samples (fig 7a). Biochem J (2014) ncbi
mouse monoclonal (mAbcam12209)
  • western blot; human; 1:1000
Abcam H3-4 antibody (abcam, ab12209) was used in western blot on human samples at 1:1000. Radiat Oncol (2014) ncbi
mouse monoclonal (mAbcam12209)
  • chromatin immunoprecipitation; human
Abcam H3-4 antibody (Abacm, ab12209) was used in chromatin immunoprecipitation on human samples . J Biol Chem (2013) ncbi
BioLegend
rat monoclonal (HTA28)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2f
BioLegend H3-4 antibody (Biolegend, 641001) was used in immunohistochemistry on mouse samples at 1:200 (fig 2f). elife (2022) ncbi
rat monoclonal (HTA28)
  • flow cytometry; mouse
BioLegend H3-4 antibody (Biolegend, 641002) was used in flow cytometry on mouse samples . Cancer Cell (2021) ncbi
rat monoclonal (HTA28)
  • flow cytometry; human; loading ...; fig s1j
BioLegend H3-4 antibody (BioLegend, 641005) was used in flow cytometry on human samples (fig s1j). Cell Rep (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-4)
  • western blot; human; 1:250; loading ...; fig 4c
Santa Cruz Biotechnology H3-4 antibody (Santa, sc-518011) was used in western blot on human samples at 1:250 (fig 4c). Sci Adv (2019) ncbi
Articles Reviewed
  1. Kuo A, Checa A, Niaudet C, Jung B, Fu Z, Wheelock C, et al. Murine endothelial serine palmitoyltransferase 1 (SPTLC1) is required for vascular development and systemic sphingolipid homeostasis. elife. 2022;11: pubmed publisher
  2. Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
  3. Pettinato A, Yoo D, VanOudenhove J, Chen Y, Cohn R, Ladha F, et al. Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep. 2021;35:109088 pubmed publisher
  4. Yi D, Nguyen H, Dinh J, Viscarra J, Xie Y, Lin F, et al. Dot1l interacts with Zc3h10 to activate Ucp1 and other thermogenic genes. elife. 2020;9: pubmed publisher
  5. Liu C, Yang Q, Zhu Q, Lu X, Li M, Hou T, et al. CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics. 2020;10:1758-1776 pubmed publisher
  6. Dremel S, DeLuca N. Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off. elife. 2019;8: pubmed publisher
  7. Bosnakovski D, da Silva M, Sunny S, Ener E, Toso E, Yuan C, et al. A novel P300 inhibitor reverses DUX4-mediated global histone H3 hyperacetylation, target gene expression, and cell death. Sci Adv. 2019;5:eaaw7781 pubmed publisher
  8. Nassa G, Salvati A, Tarallo R, Gigantino V, Alexandrova E, Memoli D, et al. Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv. 2019;5:eaav5590 pubmed publisher
  9. Lin Y, Lin Y, Huang M, Kuo P, Wu C, Lee M, et al. Tumor necrosis factor-alpha inhibitors suppress CCL2 chemokine in monocytes via epigenetic modification. Mol Immunol. 2017;83:82-91 pubmed publisher
  10. Nagaraj R, Sharpley M, Chi F, Braas D, Zhou Y, Kim R, et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell. 2017;168:210-223.e11 pubmed publisher
  11. Deng X, Shao G, Zhang H, Li C, Zhang D, Cheng L, et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene. 2017;36:1223-1231 pubmed publisher
  12. Itahana Y, Zhang J, Göke J, Vardy L, Han R, Iwamoto K, et al. Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells. Sci Rep. 2016;6:28112 pubmed publisher
  13. Carmona Mora P, Widagdo J, Tomasetig F, Canales C, Cha Y, Lee W, et al. The nuclear localization pattern and interaction partners of GTF2IRD1 demonstrate a role in chromatin regulation. Hum Genet. 2015;134:1099-115 pubmed publisher
  14. Huh Y, Sherley J. Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells. Cell Death Dis. 2014;5:e1554 pubmed publisher
  15. Arnandis T, Ferrer Vicens I, Torres L, García C, García Trevijano E, Zaragoza R, et al. Differential functions of calpain 1 during epithelial cell death and adipocyte differentiation in mammary gland involution. Biochem J. 2014;459:355-68 pubmed publisher
  16. Maroschik B, Gürtler A, Kramer A, Rößler U, Gomolka M, Hornhardt S, et al. Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines. Radiat Oncol. 2014;9:15 pubmed publisher
  17. Zhou P, Wang Z, Yuan X, Zhou C, Liu L, Wan X, et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem. 2013;288:17532-43 pubmed publisher