This is a Validated Antibody Database (VAD) review about human H2AFZ, based on 29 published articles (read how Labome selects the articles), using H2AFZ antibody in all methods. It is aimed to help Labome visitors find the most suited H2AFZ antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
H2AFZ synonym: H2A.Z-1; H2A.z; H2A/z; H2AZ; histone H2A.Z; H2AZ histone

Abcam
rabbit polyclonal
  • western blot; zebrafish ; fig s3e
Abcam H2AFZ antibody (Abcam, ab4174) was used in western blot on zebrafish samples (fig s3e). Cell (2018) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; rat; loading ...; fig 2b
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on rat samples (fig 2b). Science (2016) ncbi
rabbit monoclonal (EPR6171(2)(B))
  • western blot; human; 1:2000; fig s1
Abcam H2AFZ antibody (Abcam, ab150402) was used in western blot on human samples at 1:2000 (fig s1). Nat Commun (2016) ncbi
sheep polyclonal
  • chromatin immunoprecipitation; hamsters; fig 7
Abcam H2AFZ antibody (Abcam, ab18263) was used in chromatin immunoprecipitation on hamsters samples (fig 7). BMC Biotechnol (2016) ncbi
sheep polyclonal
  • western blot; pig; loading ...; fig 7a
In order to study sirtuins and key metabolic genes in skeletal muscle of pigs with spontaneous intrauterine growth restriction, Abcam H2AFZ antibody (Abcam, ab18262) was used in western blot on pig samples (fig 7a). Gen Comp Endocrinol (2016) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; fig 3
  • western blot; mouse; 1:1000; fig 4
Abcam H2AFZ antibody (Abcam, ab4174) was used in ChIP-Seq on mouse samples (fig 3) and in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse
Abcam H2AFZ antibody (abcam, ab4174) was used in ChIP-Seq on mouse samples . Cell Rep (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 1
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on human samples (fig 1). Epigenetics Chromatin (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 6c
  • immunoprecipitation; human; fig 6f
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on human samples (fig 6c) and in immunoprecipitation on human samples (fig 6f). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
Abcam H2AFZ antibody (Abcam, ab4174) was used in immunohistochemistry - paraffin section on mouse samples . Genet Mol Res (2014) ncbi
sheep polyclonal
  • ChIP-Seq; human
Abcam H2AFZ antibody (Abcam, ab18262) was used in ChIP-Seq on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on human samples and in western blot on human samples . Nucleic Acids Res (2014) ncbi
sheep polyclonal
  • western blot; human
Abcam H2AFZ antibody (Abcam, Ab18263) was used in western blot on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; fig 2
Abcam H2AFZ antibody (Abcam, ab4174) was used in ChIP-Seq on mouse samples (fig 2). PLoS Genet (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; zebrafish ; 1:500
Abcam H2AFZ antibody (Abcam, ab4174) was used in immunohistochemistry - paraffin section on zebrafish samples at 1:500. Epigenetics Chromatin (2013) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam H2AFZ antibody (Abcam, ab4174) was used in western blot on mouse samples . J Biol Chem (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human; fig 1
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on human samples and in western blot on human samples (fig 1). PLoS Genet (2013) ncbi
sheep polyclonal
  • chromatin immunoprecipitation; human
Abcam H2AFZ antibody (Abcam, ab18262) was used in chromatin immunoprecipitation on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
Abcam H2AFZ antibody (Abcam, ab4174) was used in chromatin immunoprecipitation on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; zebrafish
Abcam H2AFZ antibody (Abcam, ab4174) was used in western blot on zebrafish samples . Genome Res (2011) ncbi
Active Motif
rabbit polyclonal
  • ChIP-Seq; zebrafish ; fig s1a
Active Motif H2AFZ antibody (Active Motif, 39113) was used in ChIP-Seq on zebrafish samples (fig s1a). Cell (2018) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
In order to study the eigenetic role of PARP-1 as a memory marker in mitotic chromatin, Active Motif H2AFZ antibody (Active motif, 39943) was used in chromatin immunoprecipitation on human samples . Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
Active Motif H2AFZ antibody (Active Motif, 39113) was used in chromatin immunoprecipitation on mouse samples . Genes Dev (2014) ncbi
rabbit polyclonal
  • western blot; human
Active Motif H2AFZ antibody (Active Motif, 39943) was used in western blot on human samples . Mol Cell Biol (2013) ncbi
rabbit polyclonal
  • western blot; human
Active Motif H2AFZ antibody (Active Motif, 39113) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
rabbit polyclonal
  • western blot; human
Active Motif H2AFZ antibody (Active Motif, 39113) was used in western blot on human samples . Mech Ageing Dev (2012) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human
Active Motif H2AFZ antibody (Active Motif, 39113) was used in chromatin immunoprecipitation on human samples and in western blot on human samples . Epigenetics (2011) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
Active Motif H2AFZ antibody (Active Motif, 39113) was used in chromatin immunoprecipitation on mouse samples . Genome Res (2010) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 2d
Cell Signaling Technology H2AFZ antibody (CST, 2718) was used in western blot on human samples (fig 2d). Genes Dev (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology H2AFZ antibody (Cell signaling, 2718S) was used in western blot on human samples (fig 1d). Genes Dev (2017) ncbi
Articles Reviewed
  1. Murphy P, Wu S, James C, Wike C, Cairns B. Placeholder Nucleosomes Underlie Germline-to-Embryo DNA Methylation Reprogramming. Cell. 2018;172:993-1006.e13 pubmed publisher
  2. Morgan M, Rickels R, Collings C, He X, Cao K, Herz H, et al. A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation. Genes Dev. 2017;31:2003-2014 pubmed publisher
  3. Leung J, Makharashvili N, Agarwal P, Chiu L, Pourpre R, Cammarata M, et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes Dev. 2017;31:260-274 pubmed publisher
  4. Yang Y, Yamada T, Hill K, Hemberg M, Reddy N, Cho H, et al. Chromatin remodeling inactivates activity genes and regulates neural coding. Science. 2016;353:300-305 pubmed publisher
  5. Weyemi U, Redon C, Choudhuri R, Aziz T, Maeda D, Boufraqech M, et al. The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition. Nat Commun. 2016;7:10711 pubmed publisher
  6. Veith N, Ziehr H, MacLeod R, Reamon Buettner S. Mechanisms underlying epigenetic and transcriptional heterogeneity in Chinese hamster ovary (CHO) cell lines. BMC Biotechnol. 2016;16:6 pubmed publisher
  7. Chriett S, Le Huërou Luron I, Vidal H, Pirola L. Dysregulation of sirtuins and key metabolic genes in skeletal muscle of pigs with spontaneous intrauterine growth restriction is associated with alterations of circulating IGF-1. Gen Comp Endocrinol. 2016;232:76-85 pubmed publisher
  8. Tarangelo A, Lo N, Teng R, Kim E, Le L, Watson D, et al. Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression. Nat Commun. 2015;6:10028 pubmed publisher
  9. Hainer S, Fazzio T. Regulation of Nucleosome Architecture and Factor Binding Revealed by Nuclease Footprinting of the ESC Genome. Cell Rep. 2015;13:61-69 pubmed publisher
  10. Athwal R, Walkiewicz M, Baek S, Fu S, Bui M, Camps J, et al. CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells. Epigenetics Chromatin. 2015;8:2 pubmed publisher
  11. Lazorthes S, Vallot C, Briois S, Aguirrebengoa M, Thuret J, St Laurent G, et al. A vlincRNA participates in senescence maintenance by relieving H2AZ-mediated repression at the INK4 locus. Nat Commun. 2015;6:5971 pubmed publisher
  12. Wu B, Dong F, Ma X, Wang X, Lin F, Liu H. Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development. Genet Mol Res. 2014;13:5929-39 pubmed publisher
  13. Menafra R, Brinkman A, Matarese F, Franci G, Bartels S, Nguyen L, et al. Genome-wide binding of MBD2 reveals strong preference for highly methylated loci. PLoS ONE. 2014;9:e99603 pubmed publisher
  14. Lodhi N, Kossenkov A, Tulin A. Bookmarking promoters in mitotic chromatin: poly(ADP-ribose)polymerase-1 as an epigenetic mark. Nucleic Acids Res. 2014;42:7028-38 pubmed publisher
  15. Gjidoda A, Tagore M, McAndrew M, Woods A, Floer M. Nucleosomes are stably evicted from enhancers but not promoters upon induction of certain pro-inflammatory genes in mouse macrophages. PLoS ONE. 2014;9:e93971 pubmed publisher
  16. Menet J, Pescatore S, Rosbash M. CLOCK:BMAL1 is a pioneer-like transcription factor. Genes Dev. 2014;28:8-13 pubmed publisher
  17. Seo J, Kim K, Chang D, Kang H, Shin E, Kwon J, et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids Res. 2014;42:1016-25 pubmed publisher
  18. van Nuland R, Schram A, van Schaik F, Jansen P, Vermeulen M, Marc Timmers H. Multivalent engagement of TFIID to nucleosomes. PLoS ONE. 2013;8:e73495 pubmed publisher
  19. Subramanian V, Mazumder A, Surface L, Butty V, Fields P, Alwan A, et al. H2A.Z acidic patch couples chromatin dynamics to regulation of gene expression programs during ESC differentiation. PLoS Genet. 2013;9:e1003725 pubmed publisher
  20. Simonet N, Reyes M, Nardocci G, Molina A, Alvarez M. Epigenetic regulation of the ribosomal cistron seasonally modulates enrichment of H2A.Z and H2A.Zub in response to different environmental inputs in carp (Cyprinus carpio). Epigenetics Chromatin. 2013;6:22 pubmed publisher
  21. Popova E, Grigoryev S, Fan Y, Skoultchi A, Zhang S, Barnstable C. Developmentally regulated linker histone H1c promotes heterochromatin condensation and mediates structural integrity of rod photoreceptors in mouse retina. J Biol Chem. 2013;288:17895-907 pubmed publisher
  22. Dalvai M, Fleury L, Bellucci L, Kocanova S, Bystricky K. TIP48/Reptin and H2A.Z requirement for initiating chromatin remodeling in estrogen-activated transcription. PLoS Genet. 2013;9:e1003387 pubmed publisher
  23. Armour S, Bennett E, Braun C, Zhang X, McMahon S, Gygi S, et al. A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex. Mol Cell Biol. 2013;33:1487-502 pubmed publisher
  24. Bellucci L, Dalvai M, Kocanova S, Moutahir F, Bystricky K. Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z. PLoS ONE. 2013;8:e54102 pubmed publisher
  25. Lau P, Cheung P. Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity. Nucleic Acids Res. 2013;41:e49 pubmed publisher
  26. Lee K, Lau Z, Meredith C, Park J. Decrease of p400 ATPase complex and loss of H2A.Z within the p21 promoter occur in senescent IMR-90 human fibroblasts. Mech Ageing Dev. 2012;133:686-94 pubmed publisher
  27. Wu S, Zhang H, Cairns B. Genes for embryo development are packaged in blocks of multivalent chromatin in zebrafish sperm. Genome Res. 2011;21:578-89 pubmed publisher
  28. Gatta R, Mantovani R. NF-Y affects histone acetylation and H2A.Z deposition in cell cycle promoters. Epigenetics. 2011;6:526-34 pubmed
  29. Conerly M, Teves S, Diolaiti D, Ulrich M, Eisenman R, Henikoff S. Changes in H2A.Z occupancy and DNA methylation during B-cell lymphomagenesis. Genome Res. 2010;20:1383-90 pubmed publisher