This is a Validated Antibody Database (VAD) review about human GSDMD, based on 28 published articles (read how Labome selects the articles), using GSDMD antibody in all methods. It is aimed to help Labome visitors find the most suited GSDMD antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GSDMD synonym: DF5L; DFNA5L; FKSG10; GSDMDC1

Knockout validation
Abcam
domestic rabbit monoclonal (EPR19828)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s6c
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot knockout validation on mouse samples at 1:1000 (fig s6c). Immunity (2022) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5b
  • western blot knockout validation; human; fig 5c
Novus Biologicals GSDMD antibody (Novus Biologicals, NBP2-33422) was used in western blot on mouse samples (fig 5b) and in western blot knockout validation on human samples (fig 5c). Nat Chem Biol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (64-Y)
  • western blot knockout validation; human; loading ...; fig 1a
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot knockout validation on human samples (fig 1a). Proc Natl Acad Sci U S A (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-11)
  • western blot knockout validation; mouse; loading ...; fig 1k
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, H-11) was used in western blot knockout validation on mouse samples (fig 1k). Nat Commun (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (64-Y)
  • western blot knockout validation; human; loading ...; fig s2d
In order to report a role for MLKL in NLRP3 inflammasome activation, Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot knockout validation on human samples (fig s2d). J Immunol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (64-Y)
  • western blot knockout validation; mouse; fig s3e
  • western blot knockout validation; human; loading ...; fig 1b
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot knockout validation on mouse samples (fig s3e) and in western blot knockout validation on human samples (fig 1b). Nature (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:2000; fig s8
In order to study a signal amplification mechanism for inflammasomes through ASC filament formation, MilliporeSigma GSDMD antibody (Sigma, G7422) was used in western blot knockout validation on mouse samples at 1:2000 (fig s8). Nat Commun (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; loading ...; fig 1e
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples (fig 1e). Cell Death Dis (2022) ncbi
domestic rabbit monoclonal (EPR19828)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5a
  • immunocytochemistry; mouse; loading ...; fig 5b
  • western blot; mouse; loading ...; fig s1a, 4a
  • immunocytochemistry; human; loading ...; fig 5c
Abcam GSDMD antibody (Abcam, ab209845) was used in immunohistochemistry - paraffin section on mouse samples (fig 5a), in immunocytochemistry on mouse samples (fig 5b), in western blot on mouse samples (fig s1a, 4a) and in immunocytochemistry on human samples (fig 5c). Cells (2022) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; 1:2000; loading ...; fig 2f
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples at 1:2000 (fig 2f). elife (2022) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s6c
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot knockout validation on mouse samples at 1:1000 (fig s6c). Immunity (2022) ncbi
domestic rabbit monoclonal (EPR19828)
  • immunocytochemistry; mouse; 1:200; loading ...; fig 7d
  • western blot; mouse; 1:1000; loading ...; fig 3e, 7b
Abcam GSDMD antibody (Abcam, ab209845) was used in immunocytochemistry on mouse samples at 1:200 (fig 7d) and in western blot on mouse samples at 1:1000 (fig 3e, 7b). Int J Biol Sci (2022) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; loading ...; fig 6d
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples (fig 6d). Oxid Med Cell Longev (2021) ncbi
domestic rabbit monoclonal (EPR19829)
  • western blot; human; 1:1000; loading ...; fig 3j
Abcam GSDMD antibody (Abcam, ab210070) was used in western blot on human samples at 1:1000 (fig 3j). Arthritis Res Ther (2021) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; loading ...; fig 2c
  • western blot; human; loading ...; fig 2b
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples (fig 2c) and in western blot on human samples (fig 2b). Nat Cell Biol (2021) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; loading ...; fig 3a
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples (fig 3a). Cell (2021) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; 1:1000; loading ...; fig 3c
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples at 1:1000 (fig 3c). elife (2020) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; loading ...; fig 7c
Abcam GSDMD antibody (Abcam, 209845) was used in western blot on mouse samples (fig 7c). JCI Insight (2020) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; 1:500; loading ...; fig 1j, 5e, 6d
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples at 1:500 (fig 1j, 5e, 6d). Nat Commun (2018) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; 1:1000; loading ...; fig 1d
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples at 1:1000 (fig 1d). J Cell Biol (2018) ncbi
domestic rabbit monoclonal (EPR19828)
  • western blot; mouse; loading ...; fig 7f
Abcam GSDMD antibody (Abcam, ab209845) was used in western blot on mouse samples (fig 7f). Front Immunol (2018) ncbi
Novus Biologicals
domestic rabbit polyclonal
Novus Biologicals GSDMD antibody (Novus, NBP2-33422) was used . Commun Biol (2022) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1g
Novus Biologicals GSDMD antibody (Novus, NBP2-33422) was used in western blot on human samples (fig 1g). Cell Death Differ (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig s1e
  • western blot; mouse; loading ...; fig 3a
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1a
Novus Biologicals GSDMD antibody (Novus, NBP2-33422) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig s1e), in western blot on mouse samples (fig 3a) and in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1a). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3b
Novus Biologicals GSDMD antibody (Novus Biologicals, NBP2-33422) was used in western blot on human samples (fig 3b). Nat Med (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5b
  • western blot knockout validation; human; fig 5c
Novus Biologicals GSDMD antibody (Novus Biologicals, NBP2-33422) was used in western blot on mouse samples (fig 5b) and in western blot knockout validation on human samples (fig 5c). Nat Chem Biol (2017) ncbi
domestic rabbit polyclonal
Novus Biologicals GSDMD antibody (Novus Biologicals, NBP2-33422) was used . Nature (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (64-Y)
  • western blot knockout validation; human; loading ...; fig 1a
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot knockout validation on human samples (fig 1a). Proc Natl Acad Sci U S A (2019) ncbi
mouse monoclonal (64-Y)
  • western blot; human; fig 6c
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot on human samples (fig 6c). MBio (2019) ncbi
mouse monoclonal (H-11)
  • western blot knockout validation; mouse; loading ...; fig 1k
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, H-11) was used in western blot knockout validation on mouse samples (fig 1k). Nat Commun (2018) ncbi
mouse monoclonal (64-Y)
  • western blot knockout validation; human; loading ...; fig s2d
In order to report a role for MLKL in NLRP3 inflammasome activation, Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot knockout validation on human samples (fig s2d). J Immunol (2017) ncbi
mouse monoclonal (64-Y)
  • western blot knockout validation; mouse; fig s3e
  • western blot knockout validation; human; loading ...; fig 1b
Santa Cruz Biotechnology GSDMD antibody (Santa Cruz, sc-81868) was used in western blot knockout validation on mouse samples (fig s3e) and in western blot knockout validation on human samples (fig 1b). Nature (2015) ncbi
Abbexa
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 2d
  • western blot; human; loading ...; fig 5a
Abbexa GSDMD antibody (Abbexa, ABX340202) was used in western blot on mouse samples (fig 2d) and in western blot on human samples (fig 5a). Front Cell Dev Biol (2021) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 1f
  • western blot; human; loading ...; fig 1c
MilliporeSigma GSDMD antibody (Sigma-Aldrich, G7422) was used in immunohistochemistry on human samples (fig 1f) and in western blot on human samples (fig 1c). Front Cell Dev Biol (2021) ncbi
domestic rabbit polyclonal
MilliporeSigma GSDMD antibody (Sigma, G7422) was used . Nat Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 7
MilliporeSigma GSDMD antibody (Sigma, G7422) was used in western blot on human samples at 1:1000 (fig 7). PLoS Pathog (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:2000; fig s8
In order to study a signal amplification mechanism for inflammasomes through ASC filament formation, MilliporeSigma GSDMD antibody (Sigma, G7422) was used in western blot knockout validation on mouse samples at 1:2000 (fig s8). Nat Commun (2016) ncbi
Articles Reviewed
  1. Jin Y, Liu Y, Xu L, Xu J, Xiong Y, Peng Y, et al. Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis. Cell Death Dis. 2022;13:512 pubmed publisher
  2. Zhang X, Han J, Xu Y, Cai M, Gao F, Han J, et al. TSPO Deficiency Exacerbates GSDMD-Mediated Macrophage Pyroptosis in Inflammatory Bowel Disease. Cells. 2022;11: pubmed publisher
  3. Pe xf1 in Franch A, Garc xed a Vidal J, Mart xed nez C, Escolar Reina P, Mart xed nez Ojeda R, G xf3 mez A, et al. Galvanic current activates the NLRP3 inflammasome to promote Type I collagen production in tendon. elife. 2022;11: pubmed publisher
  4. Simpson D, Pang J, Weir A, Kong I, Fritsch M, Rashidi M, et al. Interferon-γ primes macrophages for pathogen ligand-induced killing via a caspase-8 and mitochondrial cell death pathway. Immunity. 2022;55:423-441.e9 pubmed publisher
  5. Wang S, Luke C, Pak S, Shi V, Chen L, Moore J, et al. SERPINB3 (SCCA1) inhibits cathepsin L and lysoptosis, protecting cervical cancer cells from chemoradiation. Commun Biol. 2022;5:46 pubmed publisher
  6. Wan L, Bai X, Zhou Q, Chen C, Wang H, Liu T, et al. The advanced glycation end-products (AGEs)/ROS/NLRP3 inflammasome axis contributes to delayed diabetic corneal wound healing and nerve regeneration. Int J Biol Sci. 2022;18:809-825 pubmed publisher
  7. Wu Z, Liao F, Luo G, Qian Y, He X, Xu W, et al. NR1D1 Deletion Induces Rupture-Prone Vulnerable Plaques by Regulating Macrophage Pyroptosis via the NF-κB/NLRP3 Inflammasome Pathway. Oxid Med Cell Longev. 2021;2021:5217572 pubmed publisher
  8. Fernández Duran I, Quintanilla A, Tarrats N, Birch J, Hari P, Millar F, et al. Cytoplasmic innate immune sensing by the caspase-4 non-canonical inflammasome promotes cellular senescence. Cell Death Differ. 2022;29:1267-1282 pubmed publisher
  9. Yang T, Sun K, Wang C, Swarnkar G, Quan S, Kress D, et al. Gasdermin D deficiency attenuates arthritis induced by traumatic injury but not autoantibody-assembled immune complexes. Arthritis Res Ther. 2021;23:286 pubmed publisher
  10. Soffientini U, Beaton N, Baweja S, Weiss E, Bihari C, Habtesion A, et al. The Lipopolysaccharide-Sensing Caspase(s)-4/11 Are Activated in Cirrhosis and Are Causally Associated With Progression to Multi-Organ Injury. Front Cell Dev Biol. 2021;9:668459 pubmed publisher
  11. Claude Taupin A, Jia J, Bhujabal Z, Garfa Traoré M, Kumar S, da Silva G, et al. ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat Cell Biol. 2021;23:846-858 pubmed publisher
  12. Chen Q, Liu X, Wang D, Zheng J, Chen L, Xie Q, et al. Periodontal Inflammation-Triggered by Periodontal Ligament Stem Cell Pyroptosis Exacerbates Periodontitis. Front Cell Dev Biol. 2021;9:663037 pubmed publisher
  13. Karki R, Sharma B, Tuladhar S, Williams E, Zalduondo L, Samir P, et al. Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes. Cell. 2021;184:149-168.e17 pubmed publisher
  14. Zhang X, Wang R, Hu D, Sun X, Fujioka H, Lundberg K, et al. Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease. Sci Adv. 2020;6: pubmed publisher
  15. Green J, Swanton T, Morris L, El Sharkawy L, Cook J, Yu S, et al. LRRC8A is essential for hypotonicity-, but not for DAMP-induced NLRP3 inflammasome activation. elife. 2020;9: pubmed publisher
  16. Tian Y, Qu S, Alam H, Williams A, Wu Z, Deng Q, et al. Peptidylarginine deiminase 2 has potential as both a biomarker and therapeutic target of sepsis. JCI Insight. 2020;5: pubmed publisher
  17. Davis M, Fairgrieve M, den Hartigh A, Yakovenko O, Duvvuri B, Lood C, et al. Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation. Proc Natl Acad Sci U S A. 2019;116:5061-5070 pubmed publisher
  18. Botto S, Abraham J, Mizuno N, Pryke K, Gall B, Landais I, et al. Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome. MBio. 2019;10: pubmed publisher
  19. Benmerzoug S, Rose S, Bounab B, Gosset D, Duneau L, Chenuet P, et al. STING-dependent sensing of self-DNA drives silica-induced lung inflammation. Nat Commun. 2018;9:5226 pubmed publisher
  20. de la Roche M, Hamilton C, Mortensen R, Jeyaprakash A, Ghosh S, Anand P. Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. J Cell Biol. 2018;217:3560-3576 pubmed publisher
  21. Johnson D, Taabazuing C, Okondo M, Chui A, Rao S, Brown F, et al. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Nat Med. 2018;24:1151-1156 pubmed publisher
  22. Qu W, Wang Y, Wu Y, Liu Y, Chen K, Liu X, et al. Triggering Receptors Expressed on Myeloid Cells 2 Promotes Corneal Resistance Against Pseudomonas aeruginosa by Inhibiting Caspase-1-Dependent Pyroptosis. Front Immunol. 2018;9:1121 pubmed publisher
  23. Chu L, Indramohan M, Ratsimandresy R, Gangopadhyay A, Morris E, Monack D, et al. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun. 2018;9:996 pubmed publisher
  24. Gutierrez K, Davis M, Daniels B, Olsen T, Ralli Jain P, Tait S, et al. MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D. J Immunol. 2017;198:2156-2164 pubmed publisher
  25. Okondo M, Johnson D, Sridharan R, Go E, Chui A, Wang M, et al. DPP8 and DPP9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis. Nat Chem Biol. 2017;13:46-53 pubmed publisher
  26. Eichholz K, Bru T, Tran T, Fernandes P, Welles H, Mennechet F, et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog. 2016;12:e1005871 pubmed publisher
  27. Dick M, Sborgi L, Rühl S, Hiller S, Broz P. ASC filament formation serves as a signal amplification mechanism for inflammasomes. Nat Commun. 2016;7:11929 pubmed publisher
  28. Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, et al. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature. 2015;526:660-5 pubmed publisher