This is a Validated Antibody Database (VAD) review about human GPNMB, based on 3 published articles (read how Labome selects the articles), using GPNMB antibody in all methods. It is aimed to help Labome visitors find the most suited GPNMB antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GPNMB synonym: HGFIN; NMB; PLCA3

Knockout validation
R&D Systems
domestic goat polyclonal
  • western blot knockout validation; mouse; fig s1b
R&D Systems GPNMB antibody (R&D, AF2330) was used in western blot knockout validation on mouse samples (fig s1b). Sci Rep (2021) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig 1c
R&D Systems GPNMB antibody (R&D, AF2550) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig 1c). Sci Rep (2021) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry knockout validation; mouse; 1:100; loading ...; fig 1c
R&D Systems GPNMB antibody (R&D, AF2550) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig 1c). Sci Rep (2021) ncbi
domestic goat polyclonal
  • western blot knockout validation; mouse; fig s1b
R&D Systems GPNMB antibody (R&D, AF2330) was used in western blot knockout validation on mouse samples (fig s1b). Sci Rep (2021) ncbi
Invitrogen
mouse monoclonal (HOST5DS)
  • flow cytometry; human; loading ...; fig s5b
Invitrogen GPNMB antibody (Thermo Fisher, 12-9838-42) was used in flow cytometry on human samples (fig s5b). Cell (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (E1Y7J)
  • immunoprecipitation; human; 1:200; loading ...; fig 2f
  • western blot; human; 1:1000; loading ...; fig 4d
In order to discuss the contribution of LINK-A to cancer, Cell Signaling Technology GPNMB antibody (Cell Signaling, 13251) was used in immunoprecipitation on human samples at 1:200 (fig 2f) and in western blot on human samples at 1:1000 (fig 4d). Nat Cell Biol (2016) ncbi
Articles Reviewed
  1. Nickl B, Qadri F, Bader M. Anti-inflammatory role of Gpnmb in adipose tissue of mice. Sci Rep. 2021;11:19614 pubmed publisher
  2. Zhang Q, He Y, Luo N, Patel S, Han Y, Gao R, et al. Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma. Cell. 2019;179:829-845.e20 pubmed publisher
  3. Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1? signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed publisher