This is a Validated Antibody Database (VAD) review about human GPI, based on 4 published articles (read how Labome selects the articles), using GPI antibody in all methods. It is aimed to help Labome visitors find the most suited GPI antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GPI synonym: AMF; GNPI; NLK; PGI; PHI; SA-36; SA36; glucose-6-phosphate isomerase; autocrine motility factor; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; oxoisomerase; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; sperm antigen-36

Abcam
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Abcam GPI antibody (Abcam, ab68643) was used in western blot on human samples at 1:1000 (fig 3b). Nat Commun (2018) ncbi
mouse monoclonal (1B7D7)
  • western blot; mouse; fig 6
Abcam GPI antibody (Abcam, ab66340) was used in western blot on mouse samples (fig 6). Mol Metab (2016) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 8e
  • western blot; mouse; loading ...; fig 7a
In order to use stable isotope labeling with amino acids to measure the proteome in dihydrotestosterone- and 17beta-estradiol-treated human proximal tubular epithelial cells, Invitrogen GPI antibody (Thermo Scientific, PA5-26787) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig 8e) and in western blot on mouse samples (fig 7a). Mol Cell Proteomics (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 1c
In order to assess if aerobic glycolysis and glutaminolysis co-operatively reduce UDP-GlcNAc biosynthesis and N-glycan branching in mouse T cell blasts, Invitrogen GPI antibody (ThermoFisher, PA5-26787) was used in western blot on mouse samples (fig 1c). elife (2017) ncbi
Articles Reviewed
  1. Persi E, Duran Frigola M, Damaghi M, Roush W, Aloy P, Cleveland J, et al. Systems analysis of intracellular pH vulnerabilities for cancer therapy. Nat Commun. 2018;9:2997 pubmed publisher
  2. Clotet S, Soler M, Riera M, Pascual J, Fang F, Zhou J, et al. Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease. Mol Cell Proteomics. 2017;16:368-385 pubmed publisher
  3. Araujo L, Khim P, Mkhikian H, Mortales C, Demetriou M. Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation. elife. 2017;6: pubmed publisher
  4. Xirouchaki C, Mangiafico S, Bate K, Ruan Z, Huang A, Tedjosiswoyo B, et al. Impaired glucose metabolism and exercise capacity with muscle-specific glycogen synthase 1 (gys1) deletion in adult mice. Mol Metab. 2016;5:221-232 pubmed publisher