This is a Validated Antibody Database (VAD) review about human GM-CSF, based on 18 published articles (read how Labome selects the articles), using GM-CSF antibody in all methods. It is aimed to help Labome visitors find the most suited GM-CSF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GM-CSF synonym: CSF; GMCSF

BioLegend
rat monoclonal (BVD2-23B6)
  • blocking or activating experiments; human; loading ...; fig 5a
BioLegend GM-CSF antibody (BioLegend, 502206) was used in blocking or activating experiments on human samples (fig 5a). Cell Death Discov (2022) ncbi
rat monoclonal (BVD2-21C11)
  • mass cytometry; human; loading ...; fig s3a
BioLegend GM-CSF antibody (Biolegend, BVD2-21C11) was used in mass cytometry on human samples (fig s3a). Proc Natl Acad Sci U S A (2017) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; loading ...; tbl 1
In order to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment, BioLegend GM-CSF antibody (Biolegend, BVD2-21C11) was used in flow cytometry on human samples (tbl 1). Cytometry A (2017) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; fig s1e
In order to investigate the role of Eomes in the retention of liver natural killer cells, BioLegend GM-CSF antibody (BioLegend, BDV-21C11) was used in flow cytometry on human samples (fig s1e). J Immunol (2016) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; loading ...; fig 3a
In order to characterize innate lymphoid cell subpopulations isolated from patients with systemic sclerosis, BioLegend GM-CSF antibody (biolegend, BVD2-21C11) was used in flow cytometry on human samples (fig 3a). J Immunol (2016) ncbi
rat monoclonal (BVD2-21C11)
  • immunoprecipitation; human; 10 ug/ml; fig 5e
In order to study the role of human head and neck squamous cell carcinoma-associated semaphorin 4d in myeloid-derived suppressor cells, BioLegend GM-CSF antibody (BioLegend, 502301) was used in immunoprecipitation on human samples at 10 ug/ml (fig 5e). J Immunol (2016) ncbi
rat monoclonal (BVD2-23B6)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend GM-CSF antibody (Biolegend, 502202) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
rat monoclonal (BVD2-21C11)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend GM-CSF antibody (Biolegend, 502304) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; 1:40
BioLegend GM-CSF antibody (BioLegend, BVD2-21C11) was used in flow cytometry on human samples at 1:40. Nat Med (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B6-2-hGMCSF)
  • western blot; human; loading ...; fig s5k
Santa Cruz Biotechnology GM-CSF antibody (Santa Cruz, sc-32753) was used in western blot on human samples (fig s5k). J Clin Invest (2022) ncbi
Invitrogen
rat monoclonal (DAVKAT)
  • flow cytometry; human; fig s12b
In order to find predictive markers for patients that will develop gastrointestinal graft-versus-host-disease, Invitrogen GM-CSF antibody (eBiosciences, DAVKAT) was used in flow cytometry on human samples (fig s12b). JCI Insight (2016) ncbi
mouse monoclonal (3092)
  • ELISA; human; fig 1
In order to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors, Invitrogen GM-CSF antibody (Endogen, M500AE) was used in ELISA on human samples (fig 1). Exp Lung Res (2002) ncbi
BD Biosciences
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; loading ...; fig 2d
  • immunohistochemistry; human; 1:50; loading ...; fig 4d
BD Biosciences GM-CSF antibody (BD, BVD2-21C11) was used in flow cytometry on human samples (fig 2d) and in immunohistochemistry on human samples at 1:50 (fig 4d). Acta Neuropathol (2021) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; loading ...; fig 1a
BD Biosciences GM-CSF antibody (BD Biosciences, BVD2-21C11) was used in flow cytometry on human samples (fig 1a). Sci Transl Med (2015) ncbi
rat monoclonal (BVD2-23B6)
  • ELISA; human; fig 1
BD Biosciences GM-CSF antibody (BD Pharmingen, 554502) was used in ELISA on human samples (fig 1). PLoS ONE (2015) ncbi
rat monoclonal (BVD2-21C11)
  • ELISA; human; fig 1
BD Biosciences GM-CSF antibody (BD Pharmingen, 554505) was used in ELISA on human samples (fig 1). PLoS ONE (2015) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; fig 3
BD Biosciences GM-CSF antibody (BD Biosciences, BVD2-21C11) was used in flow cytometry on human samples (fig 3). J Immunol (2015) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human
BD Biosciences GM-CSF antibody (BD Biosciences, BVD2-21C11) was used in flow cytometry on human samples . J Exp Med (2014) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; tbl 1
In order to study the effect of innate lymphoid cells on B cells, BD Biosciences GM-CSF antibody (BD, BVD2-1C11) was used in flow cytometry on human samples (tbl 1). Nat Immunol (2014) ncbi
rat monoclonal (BVD2-21C11)
  • flow cytometry; human; loading ...; fig 1
In order to discuss the importance of assessing immune competence in cancer patients, BD Biosciences GM-CSF antibody (BD, BVD2-21C11) was used in flow cytometry on human samples (fig 1). Cancer Immunol Immunother (2014) ncbi
Articles Reviewed
  1. Mukherjee B, Tiwari A, Palo A, Pattnaik N, Samantara S, Dixit M. Reduced expression of FRG1 facilitates breast cancer progression via GM-CSF/MEK-ERK axis by abating FRG1 mediated transcriptional repression of GM-CSF. Cell Death Discov. 2022;8:442 pubmed publisher
  2. He Y, Luo W, Liu Y, Wang Y, Ma C, Wu Q, et al. IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer. J Clin Invest. 2022;132: pubmed publisher
  3. Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  4. Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  5. Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  6. Cuff A, Robertson F, Stegmann K, Pallett L, Maini M, Davidson B, et al. Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. J Immunol. 2016;197:4283-4291 pubmed
  7. Li W, Liu L, Gomez A, Zhang J, Ramadan A, Zhang Q, et al. Proteomics analysis reveals a Th17-prone cell population in presymptomatic graft-versus-host disease. JCI Insight. 2016;1: pubmed publisher
  8. Roan F, Stoklasek T, Whalen E, Molitor J, Bluestone J, Buckner J, et al. CD4+ Group 1 Innate Lymphoid Cells (ILC) Form a Functionally Distinct ILC Subset That Is Increased in Systemic Sclerosis. J Immunol. 2016;196:2051-2062 pubmed publisher
  9. Younis R, Han K, Webb T. Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells. J Immunol. 2016;196:1419-29 pubmed publisher
  10. Li R, Rezk A, Miyazaki Y, Hilgenberg E, Touil H, Shen P, et al. Proinflammatory GM-CSF-producing B cells in multiple sclerosis and B cell depletion therapy. Sci Transl Med. 2015;7:310ra166 pubmed publisher
  11. Rodriguez J, Marchicio J, López M, Ziblat A, Elias F, Fló J, et al. PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells. PLoS ONE. 2015;10:e0117484 pubmed publisher
  12. Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
  13. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  14. Gibbons D, Fleming P, Virasami A, Michel M, Sebire N, Costeloe K, et al. Interleukin-8 (CXCL8) production is a signatory T cell effector function of human newborn infants. Nat Med. 2014;20:1206-10 pubmed publisher
  15. Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, et al. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. J Exp Med. 2014;211:1243-56 pubmed publisher
  16. Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, et al. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol. 2014;15:354-364 pubmed publisher
  17. Chang S, Kohrt H, Maecker H. Monitoring the immune competence of cancer patients to predict outcome. Cancer Immunol Immunother. 2014;63:713-9 pubmed publisher
  18. Suwa T, Hogg J, Vincent R, Mukae H, Fujii T, van Eeden S. Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells. Exp Lung Res. 2002;28:1-18 pubmed