This is a Validated Antibody Database (VAD) review about human GJB1, based on 51 published articles (read how Labome selects the articles), using GJB1 antibody in all methods. It is aimed to help Labome visitors find the most suited GJB1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GJB1 synonym: CMTX; CMTX1; CX32; gap junction beta-1 protein; GAP junction 28 kDa liver protein; connexin-32; gap junction protein, beta 1, 32kDa
Knockout validation
Invitrogen mouse monoclonal (CX-2C2) |
| In order to discuss the limitations of using antibodies to study connexin 32 expression in the central nervous system, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in immunocytochemistry knockout validation on mouse samples at 1.0 ug/ml (fig 4) and in western blot knockout validation on mouse samples at 1:250 (fig 2, 3). Cell Commun Adhes (2009) ncbi |
Invitrogen
mouse monoclonal (CX-2C2) |
| In order to examine the effect of different substrates on the self-assembly of liver organoids, Invitrogen GJB1 antibody (Invitrogen, 13-8200) was used in immunocytochemistry on human samples (fig 7). PLoS ONE (2017) ncbi |
rabbit polyclonal |
| In order to assess the contributions of Sig-1Rs and connexin 43 to the induction of central neuropathic pain during spinal cord injury, Invitrogen GJB1 antibody (Invitrogen, 71-0600) was used in western blot on mouse samples at 1:1000 (fig 4c). Neuropharmacology (2016) ncbi |
rabbit polyclonal |
| In order to investigate how prostaglandin E2 regulates breathing in young mice, Invitrogen GJB1 antibody (Invitrogen, 71-0600) was used in immunohistochemistry on mouse samples (fig 6c). elife (2016) ncbi |
mouse monoclonal (CX-2C2) |
| In order to study differential expression of astrocytic connexins by a prenatal alcohol exposure mouse model, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in western blot on mouse samples at 1:500 (fig 2). Neurobiol Dis (2016) ncbi |
mouse monoclonal (CX-2C2) |
| In order to characterize the differential modulation of connexin expression and function in human capillary endothelial cells in regards to primary tumor- and metastasis-derived colon cancer cells, Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 4). Oncotarget (2015) ncbi |
mouse monoclonal (CX-2C2) |
| In order to determine the role of growth media composition on classic EMT responses using HK-2 cells, Invitrogen GJB1 antibody (Life Technologies, CX-2C2) was used in western blot on human samples at 0.5 ug/ml. PLoS ONE (2015) ncbi |
mouse monoclonal (CX-2C2) |
| Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in blocking or activating experiments on human samples . Atherosclerosis (2014) ncbi |
mouse monoclonal (CX-2C2) |
| In order to study the effect of maternal glucocorticoid treatment on gap junction protein expression in the ovine fetal brain, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in western blot on sheep samples . Neuroscience (2014) ncbi |
rabbit polyclonal |
| In order to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus, Invitrogen GJB1 antibody (Life Technologies, 34-5700) was used in immunocytochemistry on mouse samples and in immunocytochemistry on rat samples . Eur J Neurosci (2014) ncbi |
mouse monoclonal (CX-2C2) |
| In order to investigate the expression of connexin 43 in eutopic endometrium and cultured endometrial stromal cells, Invitrogen GJB1 antibody (Life Technologies, 13-8200) was used in western blot on human samples at 1:500. Mol Hum Reprod (2014) ncbi |
mouse monoclonal (CX-2C2) |
| In order to investigate the effects of antioxidants on Nf1 loss or HRas hyper activation in mouse oligodendrocytes, Invitrogen GJB1 antibody (Invitrogen, 138200) was used in western blot on mouse samples at 1:500 (fig 1). Cell Rep (2013) ncbi |
mouse monoclonal (CX-2C2) |
| In order to isolate and characterize connexin32-enriched membrane microdomains from murine liver, Invitrogen GJB1 antibody (Zymed, Invitrogen, 13-8200) was used in western blot on mouse samples (fig s1). J Proteome Res (2013) ncbi |
rabbit polyclonal |
| In order to investigate the role of Cx37 during vasculogenesis in mice, Invitrogen GJB1 antibody (Invitrogen, 71-0600) was used in immunohistochemistry - frozen section on mouse samples . Dev Biol (2013) ncbi |
rabbit polyclonal |
| In order to study localization of connexins in the brain, Invitrogen GJB1 antibody (Invitrogen, 34-5700) was used in immunohistochemistry - frozen section on mouse samples at 3 ug/ml. Eur J Neurosci (2011) ncbi |
mouse monoclonal (CX-2C2) |
| In order to study connexin 26 and connexin 32 isoforms in the cochlea, Invitrogen GJB1 antibody (Zymed, CX-2C2) was used in western blot on mouse samples at 1:500 (fig 4). Eur J Cell Biol (2011) ncbi |
mouse monoclonal (CX-2C2) |
| In order to study connexin-32 localization in fetal rat hepatocytes, Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in immunocytochemistry on rat samples at 1:100 (fig 3). Acta Histochem (2012) ncbi |
rabbit polyclonal |
| In order to compare the growth patterns of normal and nodular hepatocytes in a transplantation system, Invitrogen GJB1 antibody (Zymed Labs, 34-5700) was used in immunohistochemistry on rat samples (fig 2). Histochem Cell Biol (2011) ncbi |
rabbit polyclonal |
| In order to discuss the use and limitations of a mesothelin-specific antibody fused to a bacterial toxin to treat mesothelioma, Invitrogen GJB1 antibody (Invitrogen, 71-0600) was used in western blot on human samples (fig 6). PLoS ONE (2011) ncbi |
mouse monoclonal (CX-2C2) |
| In order to determine the connexin composition of intracellular channels present in astrocytes and oligodendrocytes, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in immunocytochemistry on human samples at 1:100 (fig 3). Glia (2011) ncbi |
rabbit polyclonal |
| In order to determine the connexin composition of intracellular channels present in astrocytes and oligodendrocytes, Invitrogen GJB1 antibody (Zymed, 34-5700) was used in immunocytochemistry on human samples at 1:100 (fig 3). Glia (2011) ncbi |
rabbit polyclonal |
| In order to report that beta-cateninregulates the zone-specificity of a hepatocyte's gene expression profile, Invitrogen GJB1 antibody (Invitrogen, 34-5700) was used in immunohistochemistry - paraffin section on mouse samples at 1:250 (fig 4). Histochem Cell Biol (2010) ncbi |
mouse monoclonal (CX-2C2) |
| In order to test if Cx32-induced metastasis involves cancer stem cells, Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in immunohistochemistry - frozen section on human samples at 1:200 (fig 1). Int J Cancer (2011) ncbi |
mouse monoclonal (CX-2C2) |
| In order to discuss the limitations of using antibodies to study connexin 32 expression in the central nervous system, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in immunocytochemistry knockout validation on mouse samples at 1.0 ug/ml (fig 4) and in western blot knockout validation on mouse samples at 1:250 (fig 2, 3). Cell Commun Adhes (2009) ncbi |
mouse monoclonal (CX-2C2) |
| In order to investigate connexin32 in endothelial cells, Invitrogen GJB1 antibody (Zymed, CX-2C2) was used in immunocytochemistry on human samples at 1:500. Biochem Biophys Res Commun (2009) ncbi |
mouse monoclonal (CX-2C2) |
| In order to examine calcium wave propagation and connexin 26, 32 and 43 expression in normal and malignant urothelial cells, Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 3a). Cell Commun Adhes (2007) ncbi |
rabbit polyclonal | In order to determine the abundance and connexin composition of neuronal and glial gap junctions in developing and adult rat and mouse locus coeruleus, Invitrogen GJB1 antibody (Invitrogen/Zymed, 34-5700) was used . Neuroscience (2007) ncbi | |
rabbit polyclonal | In order to determine the abundance and connexin composition of neuronal and glial gap junctions in developing and adult rat and mouse locus coeruleus, Invitrogen GJB1 antibody (Invitrogen/Zymed, 71-0600) was used . Neuroscience (2007) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to study the impact of connexin32 on hepatocellular carcinoma, Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in immunocytochemistry on human samples at 1:500 and in immunohistochemistry on human samples at 1:200. Int J Cancer (2007) ncbi |
mouse monoclonal (CX-2C2) |
| In order to examine the biological responses imparted by Matrigel overlays on primary human hepatocyte cultures, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in western blot on human samples at 1:500 (fig 6). Toxicol Sci (2007) ncbi |
mouse monoclonal (CX-2C2) |
| In order to examine the contribution of connexin43 to gap junctional intercellular communication in various cell types, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in western blot on human samples at 1:500. Biotechnol J (2007) ncbi |
rabbit polyclonal | In order to examine connexin expression in chromaffin cells of normal human adrenal glands and benign and malignant pheochromocytomas, Invitrogen GJB1 antibody (ZYMED, 71-0600) was used . Ann N Y Acad Sci (2006) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to study ouabain-sensitive and -resistant MDCK cells, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in immunocytochemistry on dog samples and in western blot on dog samples . Proc Natl Acad Sci U S A (2006) ncbi |
rabbit polyclonal | In order to examine Paneth and enterochromaffin cells in Min-mice, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . Anticancer Res (2006) ncbi | |
rabbit polyclonal | In order to identify residues that are critical for connexin functions, Invitrogen GJB1 antibody (Invitrogen/Zymed, 71-0600) was used . J Cell Sci (2006) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to present the clinicopathological findings of patient with astroblastoma, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in immunohistochemistry - paraffin section on human samples at 1:500. Neuropathology (2006) ncbi |
mouse monoclonal (CX-2C2) |
| In order to evaluate gap junction protein connexin 43 as a negative marker of the pluripotency of human limbal epithelial cells, Invitrogen GJB1 antibody (Invitrogen, CX-2C2) was used in immunocytochemistry on human samples at 1:50 (fig 2). Stem Cells (2006) ncbi |
rabbit polyclonal | In order to study connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . J Physiol (2005) ncbi | |
rabbit polyclonal | In order to study rhesus monkey embryonic stem cells, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . Stem Cells (2005) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to determine the spatio-temporal expression of gap junction connexins and correlate their expression with the progression of cell cycle control in regenerating soleus muscle of Wistar rats, Invitrogen GJB1 antibody (Zymed, CX-2C2) was used in immunohistochemistry - frozen section on rat samples at 1:100. Histochem Cell Biol (2005) ncbi |
rabbit polyclonal | In order to investigate the role of connexin 43 in intestinal cancer, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . Eur J Cancer (2004) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to investigate the influence of estrogen and its receptor in connexin expression and gap junctional intercellular communication in endometrial carcinoma cells, Invitrogen GJB1 antibody (Zymed, CX-2C2) was used in immunocytochemistry on human samples at 1:500 and in western blot on human samples at 1:500 (fig 5). Oncogene (2004) ncbi |
rabbit polyclonal | In order to examine the connexin associations at oligodendrocyte/astrocyte gap junctions on oligodendrocytes in normal and Cx32 knockout mice, Invitrogen GJB1 antibody (Zymed, 34?C5700) was used . Glia (2003) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to examine the connexin associations at oligodendrocyte/astrocyte gap junctions on oligodendrocytes in normal and Cx32 knockout mice, Invitrogen GJB1 antibody (Zymed, 13?C8200) was used in immunohistochemistry - frozen section on mouse samples at 1:500, in immunohistochemistry on mouse samples and in western blot on mouse samples . Glia (2003) ncbi |
rabbit polyclonal | In order to examine the connexin associations at oligodendrocyte/astrocyte gap junctions on oligodendrocytes in normal and Cx32 knockout mice, Invitrogen GJB1 antibody (Zymed, 71?C0600) was used . Glia (2003) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to study connexins and gap junction proteins in endometrial carcinoma cells, Invitrogen GJB1 antibody (Zymed, CX-2C2) was used in immunocytochemistry on human samples (fig 4). Carcinogenesis (2003) ncbi |
rabbit polyclonal | In order to elucidate the relationship between gap junctional intercellular communication and mammary cell differentiation, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . J Cell Sci (2003) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to study connexins in retinal glial cells, Invitrogen GJB1 antibody (Zymed, 13-8200) was used in immunohistochemistry on rat samples at 10 ug/ml. J Comp Neurol (2003) ncbi |
rabbit polyclonal | In order to study connexins in retinal glial cells, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . J Comp Neurol (2003) ncbi | |
mouse monoclonal (CX-2C2) |
| In order to determine the expression of connexin-43 and 32 in patients of epilepsy, Invitrogen GJB1 antibody (Zymed Laboratories, clone CX-2C2) was used in immunohistochemistry - paraffin section on human samples at 1:250 (fig 2). Acta Neuropathol (2001) ncbi |
mouse monoclonal (CX-2C2) |
| In order to study intercellular adherens junctions and gap junctions in human gingival fibroblasts, Invitrogen GJB1 antibody (Zymed, CX-2C2) was used in western blot on human samples (fig 3). Am J Physiol Cell Physiol (2000) ncbi |
rabbit polyclonal | In order to investigate the intracellular transport, assembly, and degradation of three Charcot-Marie-Tooth disease-linked connexin32 mutants stably expressed in PC12 cells, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . Mol Biol Cell (2000) ncbi | |
rabbit polyclonal | In order to assess gap junction connectivity, Invitrogen GJB1 antibody (Zymed, 71-0600) was used . Neuroendocrinology (1999) ncbi |
Abcam
rabbit polyclonal |
| In order to test if altering the transcription programming in pancreatic ductal adenocarcinomas can revert these cancerous cells back to quiescent acinar cells, Abcam GJB1 antibody (Abcam, ab66613) was used in immunocytochemistry on human samples at 1:100. Pancreas (2015) ncbi |
MilliporeSigma
rabbit polyclonal |
| In order to investigate breast tumorigenesis due to loss of Panx1 which impairs mammary gland development at lactation, MilliporeSigma GJB1 antibody (Sigma, C3470) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 7). PLoS ONE (2016) ncbi |
rabbit polyclonal |
| In order to correlate connexin expression with tumor progression and prognosis in primary breast cancers, MilliporeSigma GJB1 antibody (Sigma-Aldrich, HPA010663) was used in immunohistochemistry on human samples at 1:30. PLoS ONE (2014) ncbi |
rabbit polyclonal |
| In order to determine if patients with loss-of-function Cx26 mutations have a greater susceptibility to impaired breast development, MilliporeSigma GJB1 antibody (Sigma-Aldrich, C3470) was used in western blot on mouse samples (fig 2). PLoS ONE (2014) ncbi |
Articles Reviewed
- Leinonen P, Aaltonen V, Koskela S, Lehenkari P, Korkiamäki T, Peltonen J. Impaired gap junction formation and intercellular calcium signaling in urinary bladder cancer cells can be improved by Gö6976. Cell Commun Adhes. 2007;14:125-36 pubmed
- Rash J, Olson C, Davidson K, Yasumura T, Kamasawa N, Nagy J. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience. 2007;147:938-56 pubmed
- Li Q, Omori Y, Nishikawa Y, Yoshioka T, Yamamoto Y, Enomoto K. Cytoplasmic accumulation of connexin32 protein enhances motility and metastatic ability of human hepatoma cells in vitro and in vivo. Int J Cancer. 2007;121:536-46 pubmed
- Page J, Johnson M, Olsavsky K, Strom S, Zarbl H, Omiecinski C. Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci. 2007;97:384-97 pubmed
- Abbaci M, Barberi Heyob M, Stines J, Blondel W, Dumas D, Guillemin F, et al. Gap junctional intercellular communication capacity by gap-FRAP technique: a comparative study. Biotechnol J. 2007;2:50-61 pubmed
- Willenberg H, Schott M, Saeger W, Tries A, Scherbaum W, Bornstein S. Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas. Ann N Y Acad Sci. 2006;1073:578-83 pubmed
- Larre I, Ponce A, Fiorentino R, Shoshani L, Contreras R, Cereijido M. Contacts and cooperation between cells depend on the hormone ouabain. Proc Natl Acad Sci U S A. 2006;103:10911-6 pubmed
- Husøy T, Knutsen H, Løberg E, Alexander J. Intestinal adenomas of Min-mice lack enterochromaffin cells, and have increased lysozyme production in non-Paneth cells. Anticancer Res. 2006;26:1797-802 pubmed
- Gemel J, Lin X, Veenstra R, Beyer E. N-terminal residues in Cx43 and Cx40 determine physiological properties of gap junction channels, but do not influence heteromeric assembly with each other or with Cx26. J Cell Sci. 2006;119:2258-68 pubmed
- Kubota T, Sato K, Arishima H, Takeuchi H, Kitai R, Nakagawa T. Astroblastoma: immunohistochemical and ultrastructural study of distinctive epithelial and probable tanycytic differentiation. Neuropathology. 2006;26:72-81 pubmed
- Chen Z, Evans W, Pflugfelder S, Li D. Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells. Stem Cells. 2006;24:1265-73 pubmed
- Valiunas V, Polosina Y, Miller H, Potapova I, Valiuniene L, Doronin S, et al. Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions. J Physiol. 2005;568:459-68 pubmed
- Behr R, Heneweer C, Viebahn C, Denker H, Thie M. Epithelial-mesenchymal transition in colonies of rhesus monkey embryonic stem cells: a model for processes involved in gastrulation. Stem Cells. 2005;23:805-16 pubmed
- Gorbe A, Becker D, Dux L, Stelkovics E, Krenacs L, Bagdi E, et al. Transient upregulation of connexin43 gap junctions and synchronized cell cycle control precede myoblast fusion in regenerating skeletal muscle in vivo. Histochem Cell Biol. 2005;123:573-83 pubmed
- Husøy T, Ølstørn H, Knutsen H, Løberg E, Cruciani V, Mikalsen S, et al. Truncated mouse adenomatous polyposis coli reduces connexin32 content and increases matrilysin secretion from Paneth cells. Eur J Cancer. 2004;40:1599-603 pubmed
- Saito T, Tanaka R, Wataba K, Kudo R, Yamasaki H. Overexpression of estrogen receptor-alpha gene suppresses gap junctional intercellular communication in endometrial carcinoma cells. Oncogene. 2004;23:1109-16 pubmed
- Nagy J, Ionescu A, Lynn B, Rash J. Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice. Glia. 2003;44:205-18 pubmed
- Nishimura M, Saito T, Yamasaki H, Kudo R. Suppression of gap junctional intercellular communication via 5' CpG island methylation in promoter region of E-cadherin gene in endometrial cancer cells. Carcinogenesis. 2003;24:1615-23 pubmed
- El Sabban M, Sfeir A, Daher M, Kalaany N, Bassam R, Talhouk R. ECM-induced gap junctional communication enhances mammary epithelial cell differentiation. J Cell Sci. 2003;116:3531-41 pubmed
- Zahs K, Kofuji P, Meier C, Dermietzel R. Connexin immunoreactivity in glial cells of the rat retina. J Comp Neurol. 2003;455:531-46 pubmed
- Aronica E, Gorter J, Jansen G, Leenstra S, Yankaya B, Troost D. Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol. 2001;101:449-59 pubmed
- Ko K, Arora P, Lee W, McCulloch C. Biochemical and functional characterization of intercellular adhesion and gap junctions in fibroblasts. Am J Physiol Cell Physiol. 2000;279:C147-57 pubmed
- VanSlyke J, Deschenes S, Musil L. Intracellular transport, assembly, and degradation of wild-type and disease-linked mutant gap junction proteins. Mol Biol Cell. 2000;11:1933-46 pubmed
- Hu L, Olson A, Weiner R, Goldsmith P. Connexin 26 expression and extensive gap junctional coupling in cultures of GT1-7 cells secreting gonadotropin-releasing hormone. Neuroendocrinology. 1999;70:221-7 pubmed
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