This is a Validated Antibody Database (VAD) review about human GJA5, based on 21 published articles (read how Labome selects the articles), using GJA5 antibody in all methods. It is aimed to help Labome visitors find the most suited GJA5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GJA5 synonym: ATFB11; CX40

Knockout validation
Alpha Diagnostics
domestic rabbit
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig s6
  • immunohistochemistry knockout validation; mouse; 1:50; loading ...; fig 1a
Alpha Diagnostics GJA5 antibody (Alpha Diagnostic International, Cx40-A) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig s6) and in immunohistochemistry knockout validation on mouse samples at 1:50 (fig 1a). Oncotarget (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 8c
  • western blot; mouse; fig s12c
Invitrogen GJA5 antibody (Invitrogen, 36-4900) was used in western blot on human samples (fig 8c) and in western blot on mouse samples (fig s12c). Int J Biol Sci (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:300; loading ...; fig s8
Invitrogen GJA5 antibody (Invitrogen, 36-4900) was used in immunohistochemistry on mouse samples at 1:300 (fig s8). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2a
  • western blot; mouse; 1:1000; loading ...; fig 4a
In order to examine connexin40 and connexin43 expression in mouse models of hypoxia and sleep apnea, Invitrogen GJA5 antibody (Life Technologies, 36-4900) was used in immunohistochemistry on mouse samples at 1:200 (fig 2a) and in western blot on mouse samples at 1:1000 (fig 4a). BMC Cell Biol (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1b
In order to determine the role of mitochondrial Cx40 in endothelial cells, Invitrogen GJA5 antibody (Thermo, 36-4900) was used in immunocytochemistry on mouse samples at 1:200 (fig 1b). Am J Physiol Cell Physiol (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 4a
In order to characterize residues important for connexin pore formation, Invitrogen GJA5 antibody (ThermoFisher, 36-4900) was used in immunocytochemistry on human samples at 1:200 (fig 4a). Channels (Austin) (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig s11
In order to determine the autonomouse beating rate adaptation in cardiomyocytes from human stem cells, Invitrogen GJA5 antibody (Life Technologies, 36-4900) was used in immunocytochemistry on human samples at 1:100 (fig s11). Nat Commun (2016) ncbi
domestic rabbit polyclonal
In order to test if H2S supplementation reduces MMP-9-induced extracellular matrix remodeling and dysfunction in diabetic kidney, Invitrogen GJA5 antibody (Life Technologies, 6-4900) was used . Nitric Oxide (2015) ncbi
mouse monoclonal (2F9A11)
  • immunocytochemistry; mouse
  • immunocytochemistry; rat
In order to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus, Invitrogen GJA5 antibody (Life Technologies, 37-8900) was used in immunocytochemistry on mouse samples and in immunocytochemistry on rat samples . Eur J Neurosci (2014) ncbi
mouse monoclonal (2F9A11)
  • immunohistochemistry; mouse; 1:200; fig 4
In order to examine the role of connexin 40 in the endothelial Ca(2+) signaling of the mouse aorta, Invitrogen GJA5 antibody (Invitrogen, 37-8900) was used in immunohistochemistry on mouse samples at 1:200 (fig 4). Cell Physiol Biochem (2013) ncbi
mouse monoclonal (2F9A11)
  • immunoprecipitation; rat; fig 5
  • immunocytochemistry; rat; 1:250; fig 5
  • western blot; rat; 1:250; fig 5
In order to study the involvement of gap junctions in calcium wave propagation between smooth muscle cells, Invitrogen GJA5 antibody (Invitrogen, 37-8900) was used in immunoprecipitation on rat samples (fig 5), in immunocytochemistry on rat samples at 1:250 (fig 5) and in western blot on rat samples at 1:250 (fig 5). Cell Commun Adhes (2012) ncbi
mouse monoclonal (2F9A11)
  • immunohistochemistry; mouse; 1:200; fig 5
In order to explore the link between the expression of endothelial connexins and nitric oxide synthase expression and function in the mouse aorta, Invitrogen GJA5 antibody (Invitrogen, 37?C8900) was used in immunohistochemistry on mouse samples at 1:200 (fig 5). Am J Physiol Heart Circ Physiol (2010) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-3)
  • flow cytometry; rat; 1:500; loading ...; fig 5a
  • immunocytochemistry; rat; 1:500; loading ...; fig 8a
  • western blot; rat; 1:1000; loading ...; fig 9a
Santa Cruz Biotechnology GJA5 antibody (Santa Cruz, sc-365107) was used in flow cytometry on rat samples at 1:500 (fig 5a), in immunocytochemistry on rat samples at 1:500 (fig 8a) and in western blot on rat samples at 1:1000 (fig 9a). Mol Med Rep (2019) ncbi
mouse monoclonal (B-3)
  • immunocytochemistry; human; fig 2h
  • western blot; human; fig 3a
Santa Cruz Biotechnology GJA5 antibody (SantaCruz, sc-365107) was used in immunocytochemistry on human samples (fig 2h) and in western blot on human samples (fig 3a). Cell Physiol Biochem (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 3c
Abcam GJA5 antibody (Abcam, ab213688) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 3c). Int J Mol Sci (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3a
Abcam GJA5 antibody (Abcam, ab213688) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 3a). Int J Mol Sci (2021) ncbi
Alpha Diagnostics
domestic rabbit
  • immunohistochemistry; mouse; 1:100; loading ...; fig 9f
Alpha Diagnostics GJA5 antibody (Alpha Diagnostic, CX40-A) was used in immunohistochemistry on mouse samples at 1:100 (fig 9f). Nat Commun (2021) ncbi
domestic rabbit
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1c
Alpha Diagnostics GJA5 antibody (Alpha Diagnostic, Cx40A) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1c). Development (2021) ncbi
domestic rabbit
  • immunohistochemistry; mouse; 1:500; loading ...; fig 2b
Alpha Diagnostics GJA5 antibody (Alpha Diagnostics, CX40-A) was used in immunohistochemistry on mouse samples at 1:500 (fig 2b). Cell (2019) ncbi
domestic rabbit
  • immunohistochemistry; mouse; 1:250; loading ...; fig 1b
  • western blot; mouse; 1:1000; loading ...; fig 7d
Alpha Diagnostics GJA5 antibody (Alpha Diagnostic, Cx40A) was used in immunohistochemistry on mouse samples at 1:250 (fig 1b) and in western blot on mouse samples at 1:1000 (fig 7d). J Clin Invest (2016) ncbi
domestic rabbit
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig s6
  • immunohistochemistry knockout validation; mouse; 1:50; loading ...; fig 1a
Alpha Diagnostics GJA5 antibody (Alpha Diagnostic International, Cx40-A) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig s6) and in immunohistochemistry knockout validation on mouse samples at 1:50 (fig 1a). Oncotarget (2016) ncbi
domestic rabbit
  • immunohistochemistry; mouse; 3.3 ug/ml
In order to study the effect of NO on the gap junctional calcium signal propagation in endothelial cells and determine the distribution of the vascular connexins in small resistance arteries, Alpha Diagnostics GJA5 antibody (Alpha Diagnostics, Cx40-A) was used in immunohistochemistry on mouse samples at 3.3 ug/ml. Cell Commun Signal (2014) ncbi
Articles Reviewed
  1. Qu K, Wang C, Huang L, Qin X, Zhang K, Zhong Y, et al. TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis. Int J Biol Sci. 2022;18:2163-2180 pubmed publisher
  2. Paštar V, Lozić M, Kelam N, Filipović N, Bernard B, Katsuyama Y, et al. Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice. Int J Mol Sci. 2021;22: pubmed publisher
  3. Quijada P, Trembley M, Misra A, Myers J, Baker C, Pérez Hernández M, et al. Coordination of endothelial cell positioning and fate specification by the epicardium. Nat Commun. 2021;12:4155 pubmed publisher
  4. Delgado C, Bu L, Zhang J, Liu F, Sall J, Liang F, et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development. 2021;148: pubmed publisher
  5. Lozi x107 M, Filipovi x107 N, Juri x107 M, Kosovi x107 I, Benzon B, x160 oli x107 I, et al. Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice. Int J Mol Sci. 2021;22: pubmed publisher
  6. Ni X, Zhang L, Ma X, Shan L, Li L, Si J, et al. β‑estradiol alleviates hypertension‑ and concanavalin A‑mediated inflammatory responses via modulation of connexins in peripheral blood lymphocytes. Mol Med Rep. 2019;19:3743-3755 pubmed publisher
  7. Das S, Goldstone A, Wang H, Farry J, D Amato G, Paulsen M, et al. A Unique Collateral Artery Development Program Promotes Neonatal Heart Regeneration. Cell. 2019;176:1128-1142.e18 pubmed publisher
  8. Fang J, Coon B, Gillis N, Chen Z, Qiu J, Chittenden T, et al. Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. Nat Commun. 2017;8:2149 pubmed publisher
  9. Gemel J, Su Z, Gileles Hillel A, Khalyfa A, Gozal D, Beyer E. Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins. BMC Cell Biol. 2017;18:7 pubmed publisher
  10. Guo R, Si R, Scott B, Makino A. Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells. Am J Physiol Cell Physiol. 2017;312:C398-C406 pubmed publisher
  11. Shekhar A, Lin X, Liu F, Zhang J, Mo H, Bastarache L, et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest. 2016;126:4444-4459 pubmed publisher
  12. Zhang Z, Chen Y, Zhang T, Guo L, Yang W, Zhang J, et al. Role of Myoendothelial Gap Junctions in the Regulation of Human Coronary Artery Smooth Muscle Cell Differentiation by Laminar Shear Stress. Cell Physiol Biochem. 2016;39:423-37 pubmed publisher
  13. Xu Q, Lin X, Matiukas A, Zhang X, Veenstra R. Specificity of the connexin W3/4 locus for functional gap junction formation. Channels (Austin). 2016;10:453-65 pubmed publisher
  14. Alonso F, Domingos Pereira S, Le Gal L, Derré L, Meda P, Jichlinski P, et al. Targeting endothelial connexin40 inhibits tumor growth by reducing angiogenesis and improving vessel perfusion. Oncotarget. 2016;7:14015-28 pubmed publisher
  15. Eng G, Lee B, Protas L, Gagliardi M, Brown K, Kass R, et al. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. Nat Commun. 2016;7:10312 pubmed publisher
  16. Kundu S, Pushpakumar S, Sen U. MMP-9- and NMDA receptor-mediated mechanism of diabetic renovascular remodeling and kidney dysfunction: hydrogen sulfide is a key modulator. Nitric Oxide. 2015;46:172-85 pubmed publisher
  17. Pogoda K, Füller M, Pohl U, Kameritsch P. NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions. Cell Commun Signal. 2014;12:33 pubmed publisher
  18. Bautista W, Rash J, Vanderpool K, Yasumura T, Nagy J. Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus. Eur J Neurosci. 2014;39:757-70 pubmed publisher
  19. Boittin F, Alonso F, Le Gal L, Allagnat F, Beny J, Haefliger J. Connexins and M3 muscarinic receptors contribute to heterogeneous Ca(2+) signaling in mouse aortic endothelium. Cell Physiol Biochem. 2013;31:166-78 pubmed publisher
  20. Halidi N, Alonso F, Burt J, Beny J, Haefliger J, Meister J. Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43. Cell Commun Adhes. 2012;19:25-37 pubmed publisher
  21. Alonso F, Boittin F, Beny J, Haefliger J. Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta. Am J Physiol Heart Circ Physiol. 2010;299:H1365-73 pubmed publisher