This is a Validated Antibody Database (VAD) review about human GEMIN5, based on 5 published articles (read how Labome selects the articles), using GEMIN5 antibody in all methods. It is aimed to help Labome visitors find the most suited GEMIN5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GEMIN5 synonym: GEMIN-5; gem-associated protein 5

Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl GEMIN5 antibody (Bethyl, A301-325A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:5000; fig 1
In order to study how altered association with SMN and U1-snRNP can cause gain and loss of function by ALS-causative mutations in FUS/TLS, Bethyl GEMIN5 antibody (Bethyl Laboratories, A301-325A) was used in western blot on human samples at 1:5000 (fig 1). Nat Commun (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (10G11)
  • western blot; human; loading ...; fig 1B
Santa Cruz Biotechnology GEMIN5 antibody (Santa Cruz, 10G11) was used in western blot on human samples (fig 1B). Nucleic Acids Res (2015) ncbi
mouse monoclonal (10G11)
  • immunoprecipitation; human; fig 4
  • immunocytochemistry; human; fig 4
Santa Cruz Biotechnology GEMIN5 antibody (Santa Cruz, sc-136200) was used in immunoprecipitation on human samples (fig 4) and in immunocytochemistry on human samples (fig 4). Nucleic Acids Res (2015) ncbi
Sigma-Aldrich
mouse monoclonal (10G11)
  • western blot; human; loading ...; fig 1a
In order to assess the role of individual Survival of MotoNeuron proteins and methylosome components in selenoprotein mRNP assembly and translation, Sigma-Aldrich GEMIN5 antibody (Sigma, 10G11) was used in western blot on human samples (fig 1a). Nucleic Acids Res (2017) ncbi
Articles Reviewed
  1. Gribling Burrer A, Leichter M, Wurth L, Huttin A, Schlotter F, Troffer Charlier N, et al. SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation. Nucleic Acids Res. 2017;45:5399-5413 pubmed publisher
  2. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  3. Yu Y, Chi B, Xia W, Gangopadhyay J, Yamazaki T, Winkelbauer Hurt M, et al. U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish. Nucleic Acids Res. 2015;43:3208-18 pubmed publisher
  4. Schertzer M, Jouravleva K, Perderiset M, Dingli F, Loew D, Le Guen T, et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Res. 2015;43:1834-47 pubmed publisher
  5. Sun S, Ling S, Qiu J, Albuquerque C, Zhou Y, Tokunaga S, et al. ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP. Nat Commun. 2015;6:6171 pubmed publisher