This is a Validated Antibody Database (VAD) review about human GATA-1, based on 24 published articles (read how Labome selects the articles), using GATA-1 antibody in all methods. It is aimed to help Labome visitors find the most suited GATA-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
GATA-1 synonym: ERYF1; GATA-1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT

Knockout validation
Santa Cruz Biotechnology
rat monoclonal (N1)
  • western blot knockout validation; human; 1:200; loading ...; fig 5c
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc- 266) was used in western blot knockout validation on human samples at 1:200 (fig 5c). Development (2019) ncbi
Santa Cruz Biotechnology
rat monoclonal (N6)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2c
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc265) was used in immunohistochemistry on mouse samples at 1:100 (fig 2c). elife (2021) ncbi
rat monoclonal (N6)
  • immunohistochemistry; mouse; loading ...
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc-265) was used in immunohistochemistry on mouse samples . elife (2020) ncbi
rat monoclonal (N1)
  • western blot knockout validation; human; 1:200; loading ...; fig 5c
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc- 266) was used in western blot knockout validation on human samples at 1:200 (fig 5c). Development (2019) ncbi
rat monoclonal (N6)
  • western blot; human; 1:1000; fig 2c
Santa Cruz Biotechnology GATA-1 antibody (Santa, sc-265) was used in western blot on human samples at 1:1000 (fig 2c). Science (2018) ncbi
rat monoclonal (N6)
  • western blot; mouse; loading ...; fig 5c
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, Sc-265) was used in western blot on mouse samples (fig 5c). J Clin Invest (2018) ncbi
rat monoclonal (N6)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1a
Santa Cruz Biotechnology GATA-1 antibody (Santa, sc-265) was used in immunohistochemistry on mouse samples at 1:100 (fig 1a). Dev Biol (2017) ncbi
rat monoclonal (N6)
  • immunohistochemistry - paraffin section; mouse; fig 7
Santa Cruz Biotechnology GATA-1 antibody (santa Cruz, sc-265) was used in immunohistochemistry - paraffin section on mouse samples (fig 7). PLoS Genet (2016) ncbi
rat monoclonal (N6)
  • immunocytochemistry; mouse; 8 ug/ml; loading ...; fig 3b
In order to perform live imaging for continuous single-cell long-term quantification of the transcription factors GATA1 and PU.1 and study how these factors influence early myeloid lineage choice, Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, N6) was used in immunocytochemistry on mouse samples at 8 ug/ml (fig 3b). Nature (2016) ncbi
rat monoclonal (N6)
  • chromatin immunoprecipitation; mouse; loading ...; fig 5a
  • chromatin immunoprecipitation; human; loading ...; fig 1
  • western blot; human; loading ...; fig s1b
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc265) was used in chromatin immunoprecipitation on mouse samples (fig 5a), in chromatin immunoprecipitation on human samples (fig 1) and in western blot on human samples (fig s1b). PLoS ONE (2016) ncbi
rat monoclonal (N6)
  • chromatin immunoprecipitation; mouse
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz Biotechnology, sc-265 X) was used in chromatin immunoprecipitation on mouse samples . Exp Biol Med (Maywood) (2016) ncbi
  • immunocytochemistry; human; 1:100; loading ...; fig 1b
  • immunocytochemistry; mouse; 1:100; loading ...; fig 1d
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, H-200) was used in immunocytochemistry on human samples at 1:100 (fig 1b) and in immunocytochemistry on mouse samples at 1:100 (fig 1d). Oncogene (2016) ncbi
rat monoclonal (N6)
  • immunohistochemistry - paraffin section; mouse; fig 7
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc-265) was used in immunohistochemistry - paraffin section on mouse samples (fig 7). Oncotarget (2015) ncbi
  • western blot; mouse; fig 1
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc13053) was used in western blot on mouse samples (fig 1). PLoS ONE (2015) ncbi
rat monoclonal (N6)
  • western blot; mouse; fig 2b
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc-265) was used in western blot on mouse samples (fig 2b). PLoS ONE (2015) ncbi
rat monoclonal (N6)
  • chromatin immunoprecipitation; human
In order to investigate the effects of UNC0638 treatment on beta-globin gene expression using ex vivo differentiated CD34(+) erythroid progenitor cells, Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz Biotechnology, sc-265) was used in chromatin immunoprecipitation on human samples . Blood (2015) ncbi
rat monoclonal (N6)
  • chromatin immunoprecipitation; human; fig 4,5,6
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc265-N6) was used in chromatin immunoprecipitation on human samples (fig 4,5,6). Blood (2015) ncbi
rat monoclonal (N6)
  • chromatin immunoprecipitation; mouse
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, sc-265) was used in chromatin immunoprecipitation on mouse samples . Mol Cell Biol (2015) ncbi
rat monoclonal (N6)
  • western blot; mouse; 1:200
Santa Cruz Biotechnology GATA-1 antibody (Santa, sc-265) was used in western blot on mouse samples at 1:200. Sci Rep (2014) ncbi
rat monoclonal (N6)
  • chromatin immunoprecipitation; mouse
  • western blot; rat
Santa Cruz Biotechnology GATA-1 antibody (Santa Cruz, N6) was used in chromatin immunoprecipitation on mouse samples and in western blot on rat samples . Mol Cell Biol (2012) ncbi
rat monoclonal (N6)
  • western blot; mouse; fig 5c
Santa Cruz Biotechnology GATA-1 antibody (Santa, sc-265) was used in western blot on mouse samples (fig 5c). J Clin Invest (2011) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3b
Abcam GATA-1 antibody (Abcam, ab28839) was used in western blot on human samples (fig 3b). Cell (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 4e
Abcam GATA-1 antibody (Abcam, ab11852) was used in ChIP-Seq on human samples (fig 4e). Nat Genet (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 4b
  • western blot; mouse; loading ...; fig 7e
In order to characterize a int-1-GATA knockout mouse, Abcam GATA-1 antibody (Abcam, ab11852) was used in chromatin immunoprecipitation on mouse samples (fig 4b) and in western blot on mouse samples (fig 7e). Nucleic Acids Res (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 7a
Cell Signaling Technology GATA-1 antibody (Cell Signaling, 4591) was used in western blot on human samples (fig 7a). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (D52H6)
  • flow cytometry; human; 1:400; loading ...; fig 2a
Cell Signaling Technology GATA-1 antibody (Cell Signaling, D52H6) was used in flow cytometry on human samples at 1:400 (fig 2a). Genome Biol (2017) ncbi
Articles Reviewed
  1. Wang F, Gervasi M, Boskovic A, Sun F, Rinaldi V, Yu J, et al. Deficient spermiogenesis in mice lacking Rlim. elife. 2021;10: pubmed publisher
  2. Richardson N, Gillot I, Gregoire E, Youssef S, DE ROOIJ D, de Bruin A, et al. Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals. elife. 2020;9: pubmed publisher
  3. Creed M, Baldeosingh R, Eberly C, Schlee C, Kim M, Cutler J, et al. PAX-SIX-EYA-DACH Network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis. Development. 2019;: pubmed publisher
  4. Grevet J, Lan X, Hamagami N, Edwards C, Sankaranarayanan L, Ji X, et al. Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells. Science. 2018;361:285-290 pubmed publisher
  5. Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  6. Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, et al. In Situ Capture of Chromatin Interactions by Biotinylated dCas9. Cell. 2017;170:1028-1043.e19 pubmed publisher
  7. Minkina A, Lindeman R, Gearhart M, Chassot A, Chaboissier M, Ghyselinck N, et al. Retinoic acid signaling is dispensable for somatic development and function in the mammalian ovary. Dev Biol. 2017;424:208-220 pubmed publisher
  8. Canver M, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern E, et al. Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017;49:625-634 pubmed publisher
  9. Zhang Y, Zhang J, An W, Wan Y, Ma S, Yin J, et al. Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation. Nucleic Acids Res. 2017;45:657-671 pubmed publisher
  10. Litzenburger U, Buenrostro J, Wu B, Shen Y, Sheffield N, Kathiria A, et al. Single-cell epigenomic variability reveals functional cancer heterogeneity. Genome Biol. 2017;18:15 pubmed publisher
  11. Saatcioglu H, Cuevas I, Castrillon D. Control of Oocyte Reawakening by Kit. PLoS Genet. 2016;12:e1006215 pubmed publisher
  12. Hoppe P, Schwarzfischer M, Loeffler D, Kokkaliaris K, Hilsenbeck O, Moritz N, et al. Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios. Nature. 2016;535:299-302 pubmed publisher
  13. Burda P, Vargova J, Curik N, Salek C, Papadopoulos G, Strouboulis J, et al. GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia. PLoS ONE. 2016;11:e0152234 pubmed publisher
  14. Braghini C, Costa F, Fedosyuk H, Neades R, Novikova L, Parker M, et al. Original Research: Generation of non-deletional hereditary persistence of fetal hemoglobin β-globin locus yeast artificial chromosome transgenic mouse models: -175 Black HPFH and -195 Brazilian HPFH. Exp Biol Med (Maywood). 2016;241:697-705 pubmed publisher
  15. Kanu N, Zhang T, Burrell R, Chakraborty A, Cronshaw J, DaCosta C, et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene. 2016;35:4009-19 pubmed publisher
  16. Minas T, Han J, Javaheri T, Hong S, Schlederer M, SaygideÄŸer Kont Y, et al. YK-4-279 effectively antagonizes EWS-FLI1 induced leukemia in a transgenic mouse model. Oncotarget. 2015;6:37678-94 pubmed publisher
  17. Golov A, Gavrilov A, Razin S. The Role of Crowding Forces in Juxtaposing β-Globin Gene Domain Remote Regulatory Elements in Mouse Erythroid Cells. PLoS ONE. 2015;10:e0139855 pubmed publisher
  18. Yashiro T, Kubo M, Ogawa H, Okumura K, Nishiyama C. PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells. PLoS ONE. 2015;10:e0137699 pubmed publisher
  19. Krivega I, Byrnes C, de Vasconcellos J, Lee Y, Kaushal M, Dean A, et al. Inhibition of G9a methyltransferase stimulates fetal hemoglobin production by facilitating LCR/γ-globin looping. Blood. 2015;126:665-72 pubmed publisher
  20. Stonestrom A, Hsu S, Jahn K, Huang P, Keller C, Giardine B, et al. Functions of BET proteins in erythroid gene expression. Blood. 2015;125:2825-34 pubmed publisher
  21. Moriguchi T, Suzuki M, Yu L, Takai J, Ohneda K, Yamamoto M. Progenitor stage-specific activity of a cis-acting double GATA motif for Gata1 gene expression. Mol Cell Biol. 2015;35:805-15 pubmed publisher
  22. Weston W, Zayas J, Perez R, George J, Jurecic R. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep. 2014;4:5199 pubmed publisher
  23. Ohmori S, Takai J, Ishijima Y, Suzuki M, Moriguchi T, Philipsen S, et al. Regulation of GATA factor expression is distinct between erythroid and mast cell lineages. Mol Cell Biol. 2012;32:4742-55 pubmed publisher
  24. Yang Y, Ahn Y, Gibbons D, Zang Y, Lin W, Thilaganathan N, et al. The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice. J Clin Invest. 2011;121:1373-85 pubmed publisher