This is a Validated Antibody Database (VAD) review about human Flt-1, based on 28 published articles (read how Labome selects the articles), using Flt-1 antibody in all methods. It is aimed to help Labome visitors find the most suited Flt-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Flt-1 synonym: FLT; FLT-1; VEGFR-1; VEGFR1

Abcam
domestic rabbit monoclonal (Y103)
  • western blot; domestic rabbit; 1:5000; loading ...; fig 8b
Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on domestic rabbit samples at 1:5000 (fig 8b). Invest Ophthalmol Vis Sci (2020) ncbi
domestic rabbit monoclonal (Y103)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4c
  • western blot; mouse; 1:1000; loading ...; fig 6
Abcam Flt-1 antibody (Abcam, ab32152) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4c) and in western blot on mouse samples at 1:1000 (fig 6). Polymers (Basel) (2020) ncbi
mouse monoclonal (Flt-1/EWC)
  • western blot; rat; 1:800; loading ...; fig 5a
Abcam Flt-1 antibody (Abcam, ab9540) was used in western blot on rat samples at 1:800 (fig 5a). Aging (Albany NY) (2020) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human; loading ...; fig 5d
Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on human samples (fig 5d). Br J Cancer (2019) ncbi
domestic rabbit monoclonal (Y103)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig e10j
Abcam Flt-1 antibody (Abcam, ab32152) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig e10j). Nature (2019) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human; fig s5c
Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on human samples (fig s5c). Cell Rep (2019) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; rat; 1:10,000; loading ...; fig 1b
In order to suggest that functional neovasculature requires NO-Tie2 and VEGF signaling, Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on rat samples at 1:10,000 (fig 1b). J Physiol (2017) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; mouse; fig 6
Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on mouse samples (fig 6). Sci Rep (2016) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human; fig 6
  • western blot; rat; fig 1
Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on human samples (fig 6) and in western blot on rat samples (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (Flt-1/EWC)
  • western blot; mouse; fig s3
Abcam Flt-1 antibody (Abcam, ab9540) was used in western blot on mouse samples (fig s3). Nat Commun (2016) ncbi
mouse monoclonal (Flt-1/EWC)
  • immunohistochemistry - paraffin section; human; 1:300; fig 2d
Abcam Flt-1 antibody (Abcam, ab9540) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig 2d). BMC Cancer (2015) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; mouse
In order to assess the role of receptor-interacting protein 2 in VEGF signalling and myocardial ischaemia/reperfusion injury, Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on mouse samples . Cardiovasc Res (2015) ncbi
mouse monoclonal (Flt-1/EWC)
  • immunohistochemistry - paraffin section; human; 1:300; fig 2
Abcam Flt-1 antibody (AbCam, ab9540) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig 2). BMC Cancer (2014) ncbi
domestic rabbit monoclonal (Y103)
  • immunocytochemistry; human
  • immunohistochemistry; human
Abcam Flt-1 antibody (Abcam, ab32152) was used in immunocytochemistry on human samples and in immunohistochemistry on human samples . Biomaterials (2014) ncbi
domestic rabbit monoclonal (Y103)
  • flow cytometry; rat
  • immunocytochemistry; rat
Abcam Flt-1 antibody (Abcam, ab32152) was used in flow cytometry on rat samples and in immunocytochemistry on rat samples . Vasc Cell (2014) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human
Abcam Flt-1 antibody (Abcam, ab32152) was used in western blot on human samples . Mol Cancer Ther (2013) ncbi
MilliporeSigma
mouse monoclonal (FLT-11)
  • flow cytometry; human; fig 2b
MilliporeSigma Flt-1 antibody (Sigma Chemical Company, V4262) was used in flow cytometry on human samples (fig 2b). Hypertension (2019) ncbi
domestic rabbit polyclonal
  • flow cytometry; human; loading ...; fig 4a
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, MilliporeSigma Flt-1 antibody (Sigma, SAB4504006) was used in flow cytometry on human samples (fig 4a), in immunohistochemistry - paraffin section on mouse samples and in western blot on mouse samples . Circ Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig s7
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, MilliporeSigma Flt-1 antibody (Sigma, SAB4504649) was used in western blot on mouse samples (fig s7). Circ Res (2017) ncbi
mouse monoclonal (Flt-19)
  • western blot; rat; 1:3000; fig 3
MilliporeSigma Flt-1 antibody (Sigma, 4762) was used in western blot on rat samples at 1:3000 (fig 3). Exp Ther Med (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-2)
  • western blot; human; loading ...; fig 3b
Santa Cruz Biotechnology Flt-1 antibody (Santa Cruz Biotechnology, sc-271789) was used in western blot on human samples (fig 3b). Breast Cancer Res (2019) ncbi
mouse monoclonal (D-2)
  • western blot; human; 1:500; fig 2b
Santa Cruz Biotechnology Flt-1 antibody (Santa Cruz, sc-271789) was used in western blot on human samples at 1:500 (fig 2b). Mol Med Rep (2018) ncbi
mouse monoclonal (D-2)
  • flow cytometry; human; fig 9
Santa Cruz Biotechnology Flt-1 antibody (Santa Cruz, sc-271789) was used in flow cytometry on human samples (fig 9). PLoS ONE (2015) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunoprecipitation; mouse; loading ...; fig 3b
  • western blot; mouse; loading ...; fig 3b
  • immunoprecipitation; human; loading ...; fig 5e
  • western blot; human; loading ...; fig 5e
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, Invitrogen Flt-1 antibody (ThermoFisher, PA5-16493) was used in immunoprecipitation on mouse samples (fig 3b), in western blot on mouse samples (fig 3b), in immunoprecipitation on human samples (fig 5e) and in western blot on human samples (fig 5e). Circ Res (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 2a
In order to evaluate the use of Tivozanib to treat patients with soft tissue sarcomas, Invitrogen Flt-1 antibody (Thermo Scientific, PA5-32408) was used in immunohistochemistry on human samples (fig 2a). Ann Oncol (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100; fig 4
In order to determine the mechanisms of bevacizumab resistance, Invitrogen Flt-1 antibody (NeoMarkers, PA5-32,408) was used in immunohistochemistry on human samples at 1:100 (fig 4). Brain Tumor Pathol (2016) ncbi
domestic rabbit polyclonal
In order to elucidate the link between CMT2D neuropathy and glycyl-tRNA synthetase, Invitrogen Flt-1 antibody (Life Technologies, 36-1100) was used . Nature (2015) ncbi
R&D Systems
domestic goat polyclonal
  • western blot; human; fig 5
R&D Systems Flt-1 antibody (R&D systems, AF321) was used in western blot on human samples (fig 5). Biol Open (2016) ncbi
domestic goat polyclonal
  • blocking or activating experiments; human; 2 ug/ml; fig 7
In order to study reprogramming of angiogenic activities of secretome by thyroid transcription factor 1, R&D Systems Flt-1 antibody (R&D Systems, AF321) was used in blocking or activating experiments on human samples at 2 ug/ml (fig 7). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • flow cytometry; human; fig 5
  • western blot; human; fig 5
Cell Signaling Technology Flt-1 antibody (Cell Signaling Technology, 2893) was used in flow cytometry on human samples (fig 5) and in western blot on human samples (fig 5). J Cell Sci (2016) ncbi
Articles Reviewed
  1. Wang Y, Gao G, Wu Y, Wang Y, Wu X, Zhou Q. S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway. Invest Ophthalmol Vis Sci. 2020;61:19 pubmed publisher
  2. Zanchetta F, Trinca R, Gomes Silva J, Breder J, Cantarutti T, Consonni S, et al. Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions. Polymers (Basel). 2020;12: pubmed publisher
  3. Chen Q, Huang M, Wu J, Jiang Q, Zheng X. Exosomes isolated from the plasma of remote ischemic conditioning rats improved cardiac function and angiogenesis after myocardial infarction through targeting Hsp70. Aging (Albany NY). 2020;12:3682-3693 pubmed publisher
  4. Chen Q, Yang C, Chen L, Zhang J, Ge W, Yuan H, et al. YY1 targets tubulin polymerisation-promoting protein to inhibit migration, invasion and angiogenesis in pancreatic cancer via p38/MAPK and PI3K/AKT pathways. Br J Cancer. 2019;121:912-921 pubmed publisher
  5. Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  6. Hori A, Shimoda M, Naoi Y, Kagara N, Tanei T, Miyake T, et al. Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer. Breast Cancer Res. 2019;21:88 pubmed publisher
  7. Njah K, Chakraborty S, Qiu B, Arumugam S, Raju A, Pobbati A, et al. A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis. Cell Rep. 2019;28:949-965.e7 pubmed publisher
  8. Deepak V, Sahu M, Yu J, Jones J, KANE M, Taylor R, et al. Retinoic Acid Is a Negative Regulator of sFLT1 Expression in Decidual Stromal Cells, and Its Levels Are Reduced in Preeclamptic Decidua. Hypertension. 2019;73:1104-1111 pubmed publisher
  9. Park G, Kim D. Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway. Mol Med Rep. 2018;17:3563-3574 pubmed publisher
  10. Ganta V, Choi M, Kutateladze A, Annex B. VEGF165b Modulates Endothelial VEGFR1-STAT3 Signaling Pathway and Angiogenesis in Human and Experimental Peripheral Arterial Disease. Circ Res. 2017;120:282-295 pubmed publisher
  11. Stone O, Carter J, Lin P, Paleolog E, Machado M, Bates D. Differential regulation of blood flow-induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling. J Physiol. 2017;595:1575-1591 pubmed publisher
  12. Agulnik M, Costa R, Milhem M, Rademaker A, Prunder B, Daniels D, et al. A phase II study of tivozanib in patients with metastatic and nonresectable soft-tissue sarcomas. Ann Oncol. 2017;28:121-127 pubmed publisher
  13. Chung S, Gillies M, Yam M, Wang Y, Shen W. Differential expression of microRNAs in retinal vasculopathy caused by selective Müller cell disruption. Sci Rep. 2016;6:28993 pubmed publisher
  14. Huang D, Zhao C, Ju R, Kumar A, Tian G, Huang L, et al. VEGF-B inhibits hyperglycemia- and Macugen-induced retinal apoptosis. Sci Rep. 2016;6:26059 pubmed publisher
  15. Semba H, Takeda N, Isagawa T, Sugiura Y, Honda K, Wake M, et al. HIF-1?-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016;7:11635 pubmed publisher
  16. Fearnley G, Smith G, Abdul Zani I, Yuldasheva N, Mughal N, Homer Vanniasinkam S, et al. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Biol Open. 2016;5:571-83 pubmed publisher
  17. Wood L, Cox N, Phelps C, Lai S, Poddar A, Talbot C, et al. Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome. Sci Rep. 2016;6:19857 pubmed publisher
  18. Lin Y, Ma Q, Lin S, Zhou H, Wen Q, Gao S, et al. Inhibitory effects of 90Sr/90Y β-irradiation on alkali burn-induced corneal neovascularization in rats. Exp Ther Med. 2016;11:409-414 pubmed
  19. Hayashi H, Al Mamun A, Sakima M, Sato M. Activator of G-protein signaling 8 is involved in VEGF-mediated signal processing during angiogenesis. J Cell Sci. 2016;129:1210-22 pubmed publisher
  20. Okamoto S, Nitta M, Maruyama T, Sawada T, Komori T, Okada Y, et al. Bevacizumab changes vascular structure and modulates the expression of angiogenic factors in recurrent malignant gliomas. Brain Tumor Pathol. 2016;33:129-36 pubmed publisher
  21. Fernández Guizán A, López Soto A, Acebes Huerta A, Huergo Zapico L, Villa Álvarez M, Núñez L, et al. Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042). PLoS ONE. 2015;10:e0140786 pubmed publisher
  22. Pinheiro C, Garcia E, Morais Santos F, Moreira M, Almeida F, Jubé L, et al. Reprogramming energy metabolism and inducing angiogenesis: co-expression of monocarboxylate transporters with VEGF family members in cervical adenocarcinomas. BMC Cancer. 2015;15:835 pubmed publisher
  23. He W, Bai G, Zhou H, Wei N, White N, Lauer J, et al. CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase. Nature. 2015;526:710-4 pubmed publisher
  24. Andersson L, Scharin Täng M, Lundqvist A, Lindbom M, Mardani I, Fogelstrand P, et al. Rip2 modifies VEGF-induced signalling and vascular permeability in myocardial ischaemia. Cardiovasc Res. 2015;107:478-86 pubmed publisher
  25. Pinheiro C, Garcia E, Morais Santos F, Scapulatempo Neto C, Mafra A, Steenbergen R, et al. Lactate transporters and vascular factors in HPV-induced squamous cell carcinoma of the uterine cervix. BMC Cancer. 2014;14:751 pubmed publisher
  26. Dirisala A, Osada K, Chen Q, Tockary T, Machitani K, Osawa S, et al. Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors. Biomaterials. 2014;35:5359-5368 pubmed publisher
  27. Kennedy E, Hakimjavadi R, Greene C, Mooney C, Fitzpatrick E, Collins L, et al. Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?. Vasc Cell. 2014;6:6 pubmed publisher
  28. Sreevalsan S, Safe S. The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells. Mol Cancer Ther. 2013;12:2483-93 pubmed publisher