This is a Validated Antibody Database (VAD) review about human Fli-1, based on 14 published articles (read how Labome selects the articles), using Fli-1 antibody in all methods. It is aimed to help Labome visitors find the most suited Fli-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Fli-1 synonym: BDPLT21; EWSR2; SIC-1; Friend leukemia integration 1 transcription factor; Ewing sarcoma breakpoint region 2; Friend leukemia virus integration 1; transcription factor ERGB

Abcam
rabbit monoclonal (EPR4646)
  • western blot; human; loading ...; fig 4b
Abcam Fli-1 antibody (Abcam, ab133485) was used in western blot on human samples (fig 4b). Science (2018) ncbi
rabbit monoclonal (EPR4646)
  • western blot; human; 1:1000; loading ...; fig 2a
  • western blot; mouse; 1:1000; fig 2b
Abcam Fli-1 antibody (Abcam, ab133485) was used in western blot on human samples at 1:1000 (fig 2a) and in western blot on mouse samples at 1:1000 (fig 2b). Mol Clin Oncol (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 6g
  • western blot; human; fig 2d
In order to learn about vulnerability in Ewing sarcoma by splicing of the EWS-FLI1 fusion transcript discovered thrugh functional genomic screening, Abcam Fli-1 antibody (Abcam, ab15289) was used in chromatin immunoprecipitation on human samples (fig 6g) and in western blot on human samples (fig 2d). Cell Rep (2016) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; fig 1
  • western blot; mouse; fig 6
Abcam Fli-1 antibody (Abcam, ab15289) was used in ChIP-Seq on mouse samples (fig 1) and in western blot on mouse samples (fig 6). Mol Cell (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 4b
  • western blot; mouse; loading ...; fig 4c
In order to study transcriptional regulation of SMAD1 and 5 in endothelial cells, Abcam Fli-1 antibody (Abcam, ab15289) was used in chromatin immunoprecipitation on mouse samples (fig 4b) and in western blot on mouse samples (fig 4c). Mol Cell Biol (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 7e
Abcam Fli-1 antibody (Abcam, 15289) was used in chromatin immunoprecipitation on mouse samples (fig 7e). EMBO Rep (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to determine the role of the lysosome in EWS-Fli-1 turnover due to proteomic analysis of the EWS-Fli-1 interactome, Abcam Fli-1 antibody (Abcam, ab15289) was used in western blot on human samples (fig 4). J Proteome Res (2014) ncbi
BD Biosciences
mouse monoclonal (G146-222)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; tbl 2
BD Biosciences Fli-1 antibody (BD Pharmingen, G146-222) was used in immunohistochemistry - paraffin section on human samples at 1:200 (tbl 2). Ann Diagn Pathol (2016) ncbi
mouse monoclonal (G146-222)
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
BD Biosciences Fli-1 antibody (BD Biosciences, G146-222) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 2). Med Mol Morphol (2016) ncbi
mouse monoclonal (G146-222)
  • immunohistochemistry; human; 1:400
In order to discuss Ewing sarcoma, BD Biosciences Fli-1 antibody (BD/Pharmingen, 554266) was used in immunohistochemistry on human samples at 1:400. Histopathology (2014) ncbi
mouse monoclonal (G146-222)
  • immunohistochemistry - paraffin section; human; 1:200
BD Biosciences Fli-1 antibody (BD Pharmingen, G146-222) was used in immunohistochemistry - paraffin section on human samples at 1:200. Histopathology (2013) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; loading ...; fig 5b
EMD Millipore Fli-1 antibody (Millipore, C219) was used in western blot on human samples (fig 5b). Oncotarget (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:20; loading ...; fig 3
EMD Millipore Fli-1 antibody (Millipore, C219) was used in western blot on human samples at 1:20 (fig 3). J Neurotrauma (2016) ncbi
Sigma-Aldrich
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
Sigma-Aldrich Fli-1 antibody (Sigma Aldrich, SAB2100822) was used in chromatin immunoprecipitation on human samples (fig 4). Oncotarget (2015) ncbi
Articles Reviewed
  1. Chong S, Dugast Darzacq C, Liu Z, Dong P, Dailey G, Cattoglio C, et al. Imaging dynamic and selective low-complexity domain interactions that control gene transcription. Science. 2018;361: pubmed publisher
  2. Xia L, Plachynta M, Liu T, Xiao X, Song J, Li X, et al. Pro-inflammatory effect of a traditional Chinese medicine formula with potent anti-cancer activity in vitro impedes tumor inhibitory potential in vivo. Mol Clin Oncol. 2016;5:717-723 pubmed publisher
  3. Rouhi A, Miller C, Grasedieck S, Reinhart S, Stolze B, Döhner H, et al. Prospective identification of resistance mechanisms to HSP90 inhibition in KRAS mutant cancer cells. Oncotarget. 2017;8:7678-7690 pubmed publisher
  4. Agaimy A, Ben Izhak O, Lorey T, Scharpf M, Rubin B. Angiosarcoma arising in association with vascular Dacron grafts and orthopedic joint prostheses: clinicopathologic, immunohistochemical, and molecular study. Ann Diagn Pathol. 2016;21:21-8 pubmed publisher
  5. Grohar P, Kim S, Rangel Rivera G, Sen N, Haddock S, Harlow M, et al. Functional Genomic Screening Reveals Splicing of the EWS-FLI1 Fusion Transcript as a Vulnerability in Ewing Sarcoma. Cell Rep. 2016;14:598-610 pubmed publisher
  6. Fidaleo M, Svetoni F, Volpe E, Miñana B, Caporossi D, Paronetto M. Genotoxic stress inhibits Ewing sarcoma cell growth by modulating alternative pre-mRNA processing of the RNA helicase DHX9. Oncotarget. 2015;6:31740-57 pubmed publisher
  7. Tajima S, Takashi Y, Ito N, Fukumoto S, Fukuyama M. ERG and FLI1 are useful immunohistochemical markers in phosphaturic mesenchymal tumors. Med Mol Morphol. 2016;49:203-209 pubmed
  8. Roe J, Mercan F, Rivera K, Pappin D, Vakoc C. BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Mol Cell. 2015;58:1028-39 pubmed publisher
  9. Willyerd F, Empey P, Philbrick A, Ikonomovic M, Puccio A, Kochanek P, et al. Expression of ATP-Binding Cassette Transporters B1 and C1 after Severe Traumatic Brain Injury in Humans. J Neurotrauma. 2016;33:226-31 pubmed publisher
  10. Marks Bluth J, Khanna A, Chandrakanthan V, Thoms J, Bee T, Eich C, et al. SMAD1 and SMAD5 Expression Is Coordinately Regulated by FLI1 and GATA2 during Endothelial Development. Mol Cell Biol. 2015;35:2165-72 pubmed publisher
  11. Liu F, Li D, Yu Y, Kang I, Cha M, Kim J, et al. Induction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71/ETV2. EMBO Rep. 2015;16:654-69 pubmed publisher
  12. Elzi D, Song M, Hakala K, Weintraub S, Shiio Y. Proteomic Analysis of the EWS-Fli-1 Interactome Reveals the Role of the Lysosome in EWS-Fli-1 Turnover. J Proteome Res. 2014;13:3783-91 pubmed publisher
  13. Milione M, Gasparini P, Sozzi G, Mazzaferro V, Ferrari A, Casali P, et al. Ewing sarcoma of the small bowel: a study of seven cases, including one with the uncommonly reported EWSR1-FEV translocation. Histopathology. 2014;64:1014-26 pubmed publisher
  14. Büttner M, Kufer V, Brunner K, Hartmann A, Amann K, Agaimy A. Benign mesenchymal tumours and tumour-like lesions in end-stage renal disease. Histopathology. 2013;62:229-36 pubmed publisher