This is a Validated Antibody Database (VAD) review about human FUS, based on 34 published articles (read how Labome selects the articles), using FUS antibody in all methods. It is aimed to help Labome visitors find the most suited FUS antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FUS synonym: ALS6; ETM4; FUS1; HNRNPP2; POMP75; TLS

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (4H11)
  • immunocytochemistry knockout validation; mouse; loading ...; fig 3a
Santa Cruz Biotechnology FUS antibody (Santa Cruz, 4H11) was used in immunocytochemistry knockout validation on mouse samples (fig 3a). J Cell Biol (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (4H11)
  • immunocytochemistry; human; 1:250; loading ...; fig e2f
  • immunohistochemistry; mouse; 1:250; loading ...; fig e2e
  • western blot; mouse; 1:500; loading ...; fig e2g
Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in immunocytochemistry on human samples at 1:250 (fig e2f), in immunohistochemistry on mouse samples at 1:250 (fig e2e) and in western blot on mouse samples at 1:500 (fig e2g). Nat Med (2022) ncbi
mouse monoclonal (4H11)
  • immunocytochemistry; mouse; 1:400; loading ...; fig s1b
Santa Cruz Biotechnology FUS antibody (Santa Cruz Biotechnology, sc-47711) was used in immunocytochemistry on mouse samples at 1:400 (fig s1b). Sci Adv (2021) ncbi
mouse monoclonal (4H11)
  • western blot; human; 1:2000; fig 6a
Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on human samples at 1:2000 (fig 6a). J Biol Chem (2020) ncbi
mouse monoclonal (D-8)
  • immunoprecipitation; human; loading ...; fig 6a
Santa Cruz Biotechnology FUS antibody (Santa, sc-373888) was used in immunoprecipitation on human samples (fig 6a). Proc Natl Acad Sci U S A (2018) ncbi
mouse monoclonal (4H11)
  • immunocytochemistry; human; loading ...; fig 2a
Santa Cruz Biotechnology FUS antibody (Santa Cruz Biotechnology Inc, sc47711) was used in immunocytochemistry on human samples (fig 2a). Cell (2018) ncbi
mouse monoclonal (4H11)
  • western blot; human; loading ...; fig 1e
Santa Cruz Biotechnology FUS antibody (SantaCruz, sc-47711) was used in western blot on human samples (fig 1e). Nucleic Acids Res (2016) ncbi
mouse monoclonal (4H11)
  • immunohistochemistry; human; loading ...; fig 2a
In order to research the effects of two familial ALS proteins on DNA damage repair, Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in immunohistochemistry on human samples (fig 2a). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (H-6)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology FUS antibody (SantaCruz, sc-373698) was used in western blot on human samples (fig 3a). Open Biol (2016) ncbi
mouse monoclonal (4H11)
  • immunoprecipitation; human; loading ...; fig 1a, 5a
  • immunocytochemistry; human; loading ...; fig 1b, 2c, 2d, 5b, 5d
  • western blot; human; loading ...; fig 1f
  • immunohistochemistry; mouse; loading ...; fig 2a, 2b
Santa Cruz Biotechnology FUS antibody (Santa Cruz Bio., sc-47711) was used in immunoprecipitation on human samples (fig 1a, 5a), in immunocytochemistry on human samples (fig 1b, 2c, 2d, 5b, 5d), in western blot on human samples (fig 1f) and in immunohistochemistry on mouse samples (fig 2a, 2b). Sci Rep (2016) ncbi
mouse monoclonal (4H11)
  • western blot; human; loading ...; fig 2d
Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on human samples (fig 2d). Nanotoxicology (2016) ncbi
mouse monoclonal (4H11)
  • immunocytochemistry knockout validation; mouse; loading ...; fig 3a
Santa Cruz Biotechnology FUS antibody (Santa Cruz, 4H11) was used in immunocytochemistry knockout validation on mouse samples (fig 3a). J Cell Biol (2016) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; loading ...; fig 3a
Santa Cruz Biotechnology FUS antibody (Santa Cruz, 4H11) was used in immunocytochemistry on mouse samples (fig 3a). J Cell Biol (2016) ncbi
mouse monoclonal (4H11)
  • immunohistochemistry - frozen section; mouse; 1:500; fig 6
  • western blot; mouse; 1:1000; fig 6
  • immunohistochemistry - paraffin section; human; 1:500; fig 8
In order to study recapitulation of SCA7 pathology and promotion of accumulation of the FUS/TLS and MBNL1 RNA-binding proteins by lentiviral vector-mediated overexpression of mutant ataxin-7, Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 6), in western blot on mouse samples at 1:1000 (fig 6) and in immunohistochemistry - paraffin section on human samples at 1:500 (fig 8). Mol Neurodegener (2016) ncbi
mouse monoclonal (4H11)
  • immunoprecipitation; human; loading ...; fig 2
In order to elucidate how Fused in Sarcoma/Translocated in Liposarcoma contributes to disease, Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in immunoprecipitation on human samples (fig 2). Biochim Biophys Acta (2016) ncbi
mouse monoclonal (4H11)
  • western blot; human; 1:100; fig 5b
  • immunoprecipitation; mouse; fig S1c
Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on human samples at 1:100 (fig 5b) and in immunoprecipitation on mouse samples (fig S1c). Nat Commun (2016) ncbi
mouse monoclonal (4H11)
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 6b
  • western blot; mouse; 1:1000; loading ...; fig 6a
Santa Cruz Biotechnology FUS antibody (SantaCruz, sc-47711) was used in immunocytochemistry on mouse samples at 1:1000 (fig 6b) and in western blot on mouse samples at 1:1000 (fig 6a). Mol Neurobiol (2017) ncbi
mouse monoclonal (4H11)
  • immunohistochemistry - paraffin section; rat; 1:200; fig 1
  • western blot; rat; 1:400; fig 1
Santa Cruz Biotechnology FUS antibody (Santa Cruz Biotechnology, sc-47711) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 1) and in western blot on rat samples at 1:400 (fig 1). Front Cell Neurosci (2015) ncbi
mouse monoclonal (4H11)
  • proximity ligation assay; human; 1:500; fig s4
  • immunocytochemistry; human; 1:500; fig 5
In order to identify protein partners of DUX4, Santa Cruz Biotechnology FUS antibody (Santa Cruz, 4H11) was used in proximity ligation assay on human samples at 1:500 (fig s4) and in immunocytochemistry on human samples at 1:500 (fig 5). PLoS ONE (2016) ncbi
mouse monoclonal (4H11)
  • ChIP-Seq; human; fig 2
  • western blot; human; 1:2000; fig s10
Santa Cruz Biotechnology FUS antibody (santa Cruz, sc-47711) was used in ChIP-Seq on human samples (fig 2) and in western blot on human samples at 1:2000 (fig s10). BMC Genomics (2015) ncbi
mouse monoclonal (4H11)
  • western blot; human; 1:2000; fig 3
In order to study induced pluripotent stem cells derived from patients with amyotrophic lateral sclerosis, Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on human samples at 1:2000 (fig 3). Dis Model Mech (2015) ncbi
mouse monoclonal (4H11)
  • cross-linking immunoprecipitation; mouse; fig 1
  • immunoprecipitation; mouse; fig 5
  • western blot; mouse; fig 5
Santa Cruz Biotechnology FUS antibody (Santa Cruz Biotechnology, 4H11) was used in cross-linking immunoprecipitation on mouse samples (fig 1), in immunoprecipitation on mouse samples (fig 5) and in western blot on mouse samples (fig 5). Genes Dev (2015) ncbi
mouse monoclonal (4H11)
  • western blot; mouse; 1:500; fig 2
In order to study how altered association with SMN and U1-snRNP can cause gain and loss of function by ALS-causative mutations in FUS/TLS, Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on mouse samples at 1:500 (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (4H11)
  • immunohistochemistry - paraffin section; human; 1:100
Santa Cruz Biotechnology FUS antibody (Santa Cruz Biotechnology, sc-47711) was used in immunohistochemistry - paraffin section on human samples at 1:100. Am J Neurodegener Dis (2014) ncbi
mouse monoclonal (4H11)
  • western blot; human; 1:800; loading ...; fig 2b
Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on human samples at 1:800 (fig 2b). Neurobiol Aging (2015) ncbi
mouse monoclonal (4H11)
  • western blot; human; fig 2
In order to study the role of loss of spliceosome integrity in the development of amyotrophic lateral sclerosis and spinal muscular atrophy, Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in western blot on human samples (fig 2). EMBO Mol Med (2013) ncbi
mouse monoclonal (4H11)
  • chromatin immunoprecipitation; human
  • western blot; human
Santa Cruz Biotechnology FUS antibody (Santa Cruz, sc-47711) was used in chromatin immunoprecipitation on human samples and in western blot on human samples . EMBO J (2012) ncbi
Novus Biologicals
domestic rabbit polyclonal (PC10)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 6e, s16d
  • immunohistochemistry - frozen section; human; 1:500; loading ...; fig 6e, s16d
Novus Biologicals FUS antibody (Novus, NB100-565) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 6e, s16d) and in immunohistochemistry - frozen section on human samples at 1:500 (fig 6e, s16d). iScience (2021) ncbi
domestic rabbit polyclonal (PC10)
  • western blot; mouse; 1:5000; loading ...
Novus Biologicals FUS antibody (Novus Biologicals, NB100-565) was used in western blot on mouse samples at 1:5000. Sci Adv (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:50,000; loading ...; fig 5d
  • immunocytochemistry; mouse; 1:100; loading ...; fig 5b
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5e
Novus Biologicals FUS antibody (Novus Biologicals, NB100-2599) was used in western blot on human samples at 1:50,000 (fig 5d), in immunocytochemistry on mouse samples at 1:100 (fig 5b) and in immunohistochemistry on mouse samples at 1:100 (fig 5e). Cell Death Dis (2021) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 3a
  • western blot; mouse; 1:1000; loading ...; fig 1b
In order to test if neuronal loss is caused by toxic gain-of-function cytoplasmic aggregates or loss of nuclear RNA-binding protein function in patients with sporadic and familial amyotrophic lateral sclerosis, Invitrogen FUS antibody (Thermo Fischer Scientific, PA5-23696) was used in immunohistochemistry on mouse samples (fig 3a) and in western blot on mouse samples at 1:1000 (fig 1b). Brain (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:10,000; loading ...; fig s1a
In order to characterize the components of stress granules, MilliporeSigma FUS antibody (Sigma-Aldrich, HPA008784) was used in western blot on human samples at 1:10,000 (fig s1a). EMBO J (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; fig 5
  • western blot; human; fig 6
In order to suggest that the clinical feature of choreoathetosis in behavioral variant of frontotemporal dementia might be associated with the basophilic inclusion body disease subtype of frontotemporal lobar degeneration, MilliporeSigma FUS antibody (Sigma-Aldrich, HPA008784) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 5) and in western blot on human samples (fig 6). Acta Neuropathol Commun (2016) ncbi
domestic rabbit polyclonal
MilliporeSigma FUS antibody (Sigma-Aldrich, HPA008784) was used . Front Cell Neurosci (2015) ncbi
domestic rabbit polyclonal
In order to report the neuropathologic autopsy findings of a man with the rare Tyr69His transthyretin gene variant, MilliporeSigma FUS antibody (Sigma, HPA008784) was used . Acta Neuropathol Commun (2015) ncbi
BD Biosciences
mouse monoclonal (15/TLS)
  • western blot; human; loading ...; fig 3a
In order to report the proteome of CADA-treated SUP-T1 human CD4+ T lymphocytes, BD Biosciences FUS antibody (BD Biosciences, 15) was used in western blot on human samples (fig 3a). Mol Cell Proteomics (2017) ncbi
mouse monoclonal (15/TLS)
  • western blot; human; 1:2000; fig 1
BD Biosciences FUS antibody (BD bioscience, 611385) was used in western blot on human samples at 1:2000 (fig 1). Cell Biosci (2016) ncbi
mouse monoclonal (15/TLS)
  • immunohistochemistry; human; 1:500; fig 3
BD Biosciences FUS antibody (BD Biosciences, 611385) was used in immunohistochemistry on human samples at 1:500 (fig 3). Amyotroph Lateral Scler Frontotemporal Degener (2015) ncbi
Articles Reviewed
  1. Korobeynikov V, Lyashchenko A, Blanco Redondo B, Jafar Nejad P, Shneider N. Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis. Nat Med. 2022;28:104-116 pubmed publisher
  2. Devoy A, Price G, De Giorgio F, Bunton Stasyshyn R, Thompson D, Gasco S, et al. Generation and analysis of innovative genomically humanized knockin SOD1, TARDBP (TDP-43), and FUS mouse models. iScience. 2021;24:103463 pubmed publisher
  3. Birsa N, Ule A, Garone M, Tsang B, Mattedi F, Chong P, et al. FUS-ALS mutants alter FMRP phase separation equilibrium and impair protein translation. Sci Adv. 2021;7: pubmed publisher
  4. Tripathi P, Guo H, Dreser A, Yamoah A, Sechi A, Jesse C, et al. Pathomechanisms of ALS8: altered autophagy and defective RNA binding protein (RBP) homeostasis due to the VAPB P56S mutation. Cell Death Dis. 2021;12:466 pubmed publisher
  5. S xe9 vigny M, Bourdeau Julien I, Venkatasubramani J, Hui J, Dutchak P, Sephton C. FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem. 2020;295:18459-18473 pubmed publisher
  6. Castanotto D, Zhang X, Alluin J, Zhang X, Rüger J, Armstrong B, et al. A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus. Proc Natl Acad Sci U S A. 2018;115:E5756-E5765 pubmed publisher
  7. Qamar S, Wang G, Randle S, Ruggeri F, Varela J, Lin J, et al. FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions. Cell. 2018;173:720-734.e15 pubmed publisher
  8. Matějů D, Franzmann T, Patel A, Kopach A, Boczek E, Maharana S, et al. An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function. EMBO J. 2017;36:1669-1687 pubmed publisher
  9. Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  10. Fletcher C, Godfrey J, Shibakawa A, Bushell M, Bevan C. A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis. Nucleic Acids Res. 2016;: pubmed
  11. Hill S, Mordes D, Cameron L, Neuberg D, Landini S, Eggan K, et al. Two familial ALS proteins function in prevention/repair of transcription-associated DNA damage. Proc Natl Acad Sci U S A. 2016;113:E7701-E7709 pubmed
  12. Samson A, Ho B, Au A, Schoenwaelder S, Smyth M, Bottomley S, et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol. 2016;6: pubmed
  13. Yamaguchi A, Takanashi K. FUS interacts with nuclear matrix-associated protein SAFB1 as well as Matrin3 to regulate splicing and ligand-mediated transcription. Sci Rep. 2016;6:35195 pubmed publisher
  14. Klein G, Mathé C, Biola Clier M, Devineau S, Drouineau E, Hatem E, et al. RNA-binding proteins are a major target of silica nanoparticles in cell extracts. Nanotoxicology. 2016;10:1555-1564 pubmed
  15. West J, Mito M, Kurosaka S, Takumi T, Tanegashima C, Chujo T, et al. Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization. J Cell Biol. 2016;214:817-30 pubmed publisher
  16. Alves S, Marais T, Biferi M, Furling D, Marinello M, El Hachimi K, et al. Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins. Mol Neurodegener. 2016;11:58 pubmed publisher
  17. Kamelgarn M, Chen J, Kuang L, Arenas A, Zhai J, Zhu H, et al. Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS. Biochim Biophys Acta. 2016;1862:2004-14 pubmed publisher
  18. Kapeli K, Pratt G, Vu A, Hutt K, Martinez F, Sundararaman B, et al. Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analyses. Nat Commun. 2016;7:12143 pubmed publisher
  19. Shiihashi G, Ito D, Yagi T, Nihei Y, Ebine T, Suzuki N. Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice. Brain. 2016;139:2380-94 pubmed publisher
  20. Kawakami I, Kobayashi Z, Arai T, Yokota O, Nonaka T, Aoki N, et al. Chorea as a clinical feature of the basophilic inclusion body disease subtype of fused-in-sarcoma-associated frontotemporal lobar degeneration. Acta Neuropathol Commun. 2016;4:36 pubmed publisher
  21. Maharjan N, Künzli C, Buthey K, Saxena S. C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress. Mol Neurobiol. 2017;54:3062-3077 pubmed publisher
  22. Schoen M, Reichel J, Demestre M, Putz S, Deshpande D, Proepper C, et al. Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons. Front Cell Neurosci. 2015;9:496 pubmed publisher
  23. Yoneda R, Suzuki S, Mashima T, Kondo K, Nagata T, Katahira M, et al. The binding specificity of Translocated in LipoSarcoma/FUsed in Sarcoma with lncRNA transcribed from the promoter region of cyclin D1. Cell Biosci. 2016;6:4 pubmed publisher
  24. Ansseau E, Eidahl J, Lancelot C, Tassin A, Mattéotti C, Yip C, et al. Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle Differentiation. PLoS ONE. 2016;11:e0146893 pubmed publisher
  25. Luo Y, Blechingberg J, Fernandes A, Li S, Fryland T, Børglum A, et al. EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. BMC Genomics. 2015;16:929 pubmed publisher
  26. Ziskin J, Greicius M, Zhu W, Okumu A, Adams C, Plowey E. Neuropathologic analysis of Tyr69His TTR variant meningovascular amyloidosis with dementia. Acta Neuropathol Commun. 2015;3:43 pubmed publisher
  27. Lenzi J, De Santis R, de Turris V, Morlando M, Laneve P, Calvo A, et al. ALS mutant FUS proteins are recruited into stress granules in induced pluripotent stem cell-derived motoneurons. Dis Model Mech. 2015;8:755-66 pubmed publisher
  28. Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, et al. Position-specific binding of FUS to nascent RNA regulates mRNA length. Genes Dev. 2015;29:1045-57 pubmed publisher
  29. Robinson H, Deykin A, Bronovitsky E, Ovchinnikov R, Ustyugov A, Shelkovnikova T, et al. Early lethality and neuronal proteinopathy in mice expressing cytoplasm-targeted FUS that lacks the RNA recognition motif. Amyotroph Lateral Scler Frontotemporal Degener. 2015;16:402-9 pubmed publisher
  30. Sun S, Ling S, Qiu J, Albuquerque C, Zhou Y, Tokunaga S, et al. ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP. Nat Commun. 2015;6:6171 pubmed publisher
  31. Nakamura M, Murray M, Lin W, Kusaka H, Dickson D. Optineurin immunoreactivity in neuronal and glial intranuclear inclusions in adult-onset neuronal intranuclear inclusion disease. Am J Neurodegener Dis. 2014;3:93-102 pubmed
  32. Wang T, Jiang X, Chen G, Xu J. Interaction of amyotrophic lateral sclerosis/frontotemporal lobar degeneration-associated fused-in-sarcoma with proteins involved in metabolic and protein degradation pathways. Neurobiol Aging. 2015;36:527-35 pubmed publisher
  33. Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher
  34. Morlando M, Dini Modigliani S, Torrelli G, Rosa A, Di Carlo V, Caffarelli E, et al. FUS stimulates microRNA biogenesis by facilitating co-transcriptional Drosha recruitment. EMBO J. 2012;31:4502-10 pubmed publisher