This is a Validated Antibody Database (VAD) review about human FTO, based on 17 published articles (read how Labome selects the articles), using FTO antibody in all methods. It is aimed to help Labome visitors find the most suited FTO antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FTO synonym: ALKBH9; BMIQ14; GDFD

Knockout validation
Abcam
domestic rabbit monoclonal (EPR6894)
  • western blot knockout validation; human; 1:1000; loading ...; fig 3b
Abcam FTO antibody (Abcam, ab126605) was used in western blot knockout validation on human samples at 1:1000 (fig 3b). J Mol Biol (2018) ncbi
Abcam
domestic rabbit monoclonal (EPR6894)
  • immunocytochemistry knockout validation; human; loading ...; fig s3a
  • western blot knockout validation; human; loading ...; fig s3b
Abcam FTO antibody (Abcam, ab126605) was used in immunocytochemistry knockout validation on human samples (fig s3a) and in western blot knockout validation on human samples (fig s3b). Nature (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-3)
  • western blot knockout validation; human; 1:500; loading ...; fig 1a
  • immunoprecipitation; human; loading ...; fig 7h
Santa Cruz Biotechnology FTO antibody (Santa Cruz, SC-271713) was used in western blot knockout validation on human samples at 1:500 (fig 1a) and in immunoprecipitation on human samples (fig 7h). Nat Commun (2021) ncbi
Abcam
mouse monoclonal (5-2H10)
  • western blot; mouse; loading ...; fig 1e
Abcam FTO antibody (Abcam, ab92821) was used in western blot on mouse samples (fig 1e). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR6894)
  • western blot; human; 1:1000; loading ...; fig 3e
Abcam FTO antibody (Abcam, ab126605) was used in western blot on human samples at 1:1000 (fig 3e). Mol Ther Nucleic Acids (2021) ncbi
mouse monoclonal (5-2H10)
  • proximity ligation assay; human; 1:200; fig 8j
  • immunohistochemistry; human; loading ...; fig 4a
Abcam FTO antibody (Abcam, ab92821) was used in proximity ligation assay on human samples at 1:200 (fig 8j) and in immunohistochemistry on human samples (fig 4a). Nat Commun (2021) ncbi
domestic rabbit monoclonal (EPR6895)
  • western blot; human; 1:1000; loading ...; fig 5k
Abcam FTO antibody (Abcam, ab124892) was used in western blot on human samples at 1:1000 (fig 5k). Hepatology (2021) ncbi
domestic rabbit monoclonal (EPR6895)
  • western blot; human; 1:1000; loading ...; fig s5
Abcam FTO antibody (Abcam, ab124892) was used in western blot on human samples at 1:1000 (fig s5). Nat Microbiol (2020) ncbi
domestic rabbit monoclonal (EPR6894)
  • western blot; human; 1:5000
Abcam FTO antibody (Abcam, ab126605) was used in western blot on human samples at 1:5000. elife (2019) ncbi
domestic rabbit monoclonal (EPR6895)
  • western blot; human; loading ...; fig s7d
Abcam FTO antibody (Abcam, ab124892) was used in western blot on human samples (fig s7d). Cell (2019) ncbi
domestic rabbit monoclonal (EPR6894)
  • immunohistochemistry; human; 1:100; loading ...; fig 1a
Abcam FTO antibody (Abcam, ab126605) was used in immunohistochemistry on human samples at 1:100 (fig 1a). Nat Commun (2019) ncbi
mouse monoclonal (5-2H10)
  • western blot; mouse; loading ...; fig s1a
Abcam FTO antibody (Abcam, ab92821) was used in western blot on mouse samples (fig s1a). Nat Chem Biol (2019) ncbi
domestic rabbit monoclonal (EPR6895)
Abcam FTO antibody (Abcam, ab124892) was used . Nucleic Acids Res (2019) ncbi
domestic rabbit monoclonal (EPR6895)
  • western blot; human; fig 5a
Abcam FTO antibody (Abcam, ab124892) was used in western blot on human samples (fig 5a). Cell (2018) ncbi
domestic rabbit monoclonal (EPR6894)
  • western blot knockout validation; human; 1:1000; loading ...; fig 3b
Abcam FTO antibody (Abcam, ab126605) was used in western blot knockout validation on human samples at 1:1000 (fig 3b). J Mol Biol (2018) ncbi
domestic rabbit monoclonal (EPR6894)
  • immunocytochemistry knockout validation; human; loading ...; fig s3a
  • western blot knockout validation; human; loading ...; fig s3b
Abcam FTO antibody (Abcam, ab126605) was used in immunocytochemistry knockout validation on human samples (fig s3a) and in western blot knockout validation on human samples (fig s3b). Nature (2017) ncbi
domestic rabbit monoclonal (EPR6894)
  • immunocytochemistry; human; fig S2 A
Abcam FTO antibody (AbCam, ab126605) was used in immunocytochemistry on human samples (fig S2 A). PLoS ONE (2017) ncbi
domestic rabbit monoclonal (EPR6895)
  • western blot; human; loading ...; fig s3d
In order to research the regulation of mRNA stability, Abcam FTO antibody (Abcam, ab124892) was used in western blot on human samples (fig s3d). Nature (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-3)
  • western blot knockout validation; human; 1:500; loading ...; fig 1a
  • immunoprecipitation; human; loading ...; fig 7h
Santa Cruz Biotechnology FTO antibody (Santa Cruz, SC-271713) was used in western blot knockout validation on human samples at 1:500 (fig 1a) and in immunoprecipitation on human samples (fig 7h). Nat Commun (2021) ncbi
mouse monoclonal (C-3)
  • western blot; human; 1:200; loading ...; fig 1c
Santa Cruz Biotechnology FTO antibody (Santa Cruz, SC-271713) was used in western blot on human samples at 1:200 (fig 1c). Nat Commun (2019) ncbi
mouse monoclonal (C-3)
  • western blot; mouse; loading ...; fig 3a
Santa Cruz Biotechnology FTO antibody (Santa, sc-271713) was used in western blot on mouse samples (fig 3a). Nucleic Acids Res (2019) ncbi
mouse monoclonal (C-3)
  • immunocytochemistry; human; fig S2 A
Santa Cruz Biotechnology FTO antibody (Santa Cruz, sc-271713) was used in immunocytochemistry on human samples (fig S2 A). PLoS ONE (2017) ncbi
Abnova
domestic goat polyclonal
  • western blot; mouse; fig 1
Abnova FTO antibody (Abnova, PAB11419) was used in western blot on mouse samples (fig 1). Sci Rep (2016) ncbi
Articles Reviewed
  1. Lü Z, Liu H, Song N, Liang Y, Zhu J, Chen J, et al. METTL14 aggravates podocyte injury and glomerulopathy progression through N6-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12:881 pubmed publisher
  2. Zeng J, Zhang H, Tan Y, Wang Z, Li Y, Yang X. m6A demethylase FTO suppresses pancreatic cancer tumorigenesis by demethylating PJA2 and inhibiting Wnt signaling. Mol Ther Nucleic Acids. 2021;25:277-292 pubmed publisher
  3. Cui Y, Yang S, Wei J, Shea C, Zhong W, Wang F, et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat Commun. 2021;12:2183 pubmed publisher
  4. Niu Y, Lin Z, Wan A, Sun L, Yan S, Liang H, et al. Loss-of-function genetic screening identifies ALDOA as an essential driver for liver cancer cell growth under hypoxia. Hepatology. 2021;: pubmed publisher
  5. Lu M, Zhang Z, Xue M, Zhao B, Harder O, Li A, et al. N6-methyladenosine modification enables viral RNA to escape recognition by RNA sensor RIG-I. Nat Microbiol. 2020;5:584-598 pubmed publisher
  6. Baquero Perez B, Antanaviciute A, Yonchev I, Carr I, Wilson S, Whitehouse A. The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus. elife. 2019;8: pubmed publisher
  7. Garcia Campos M, Edelheit S, Toth U, Safra M, Shachar R, Viukov S, et al. Deciphering the "m6A Code" via Antibody-Independent Quantitative Profiling. Cell. 2019;: pubmed publisher
  8. Yang S, Wei J, Cui Y, Park G, Shah P, Deng Y, et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. Nat Commun. 2019;10:2782 pubmed publisher
  9. Song T, Yang Y, Wei H, Xie X, Lu J, Zeng Q, et al. Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation. Nucleic Acids Res. 2019;: pubmed publisher
  10. Mauer J, Sindelar M, Despic V, Guez T, Hawley B, Vasseur J, et al. FTO controls reversible m6Am RNA methylation during snRNA biogenesis. Nat Chem Biol. 2019;15:340-347 pubmed publisher
  11. Hao H, Hao S, Chen H, Chen Z, Zhang Y, Wang J, et al. N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. Nucleic Acids Res. 2019;47:362-374 pubmed publisher
  12. Su R, Dong L, Li C, Nachtergaele S, Wunderlich M, Qing Y, et al. R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling. Cell. 2018;172:90-105.e23 pubmed publisher
  13. Zhu T, Yong X, Xia D, Widagdo J, Anggono V. Ubiquitination Regulates the Proteasomal Degradation and Nuclear Translocation of the Fat Mass and Obesity-Associated (FTO) Protein. J Mol Biol. 2018;430:363-371 pubmed publisher
  14. Xiang Y, Laurent B, Hsu C, Nachtergaele S, Lu Z, Sheng W, et al. RNA m6A methylation regulates the ultraviolet-induced DNA damage response. Nature. 2017;543:573-576 pubmed publisher
  15. Aas A, Isakson P, Bindesbøll C, Alemu E, Klungland A, Simonsen A. Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy. PLoS ONE. 2017;12:e0168182 pubmed publisher
  16. Mauer J, Luo X, Blanjoie A, Jiao X, Grozhik A, Patil D, et al. Reversible methylation of m6Am in the 5' cap controls mRNA stability. Nature. 2017;541:371-375 pubmed publisher
  17. Wang C, Shie S, Tsai M, Yang C, Hung K, Wang C, et al. FTO modulates fibrogenic responses in obstructive nephropathy. Sci Rep. 2016;6:18874 pubmed publisher