This is a Validated Antibody Database (VAD) review about human FLT3, based on 19 published articles (read how Labome selects the articles), using FLT3 antibody in all methods. It is aimed to help Labome visitors find the most suited FLT3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FLT3 synonym: CD135; FLK-2; FLK2; STK1; receptor-type tyrosine-protein kinase FLT3; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; growth factor receptor tyrosine kinase type III; stem cell tyrosine kinase 1

BioLegend
mouse monoclonal (BV10A4H2)
  • flow cytometry; human; fig 1a
In order to study the involvement of Notch signaling in NK cell lineage determination, BioLegend FLT3 antibody (biolegend, BV10A4H2) was used in flow cytometry on human samples (fig 1a). J Immunol (2017) ncbi
mouse monoclonal (BV10A4H2)
  • flow cytometry; human; 1:25; loading ...; fig 1b
In order to explore the role of thrombopoietin signaling in the human primitive hematopoietic stem cell compartment, BioLegend FLT3 antibody (BioLegend, BV10A4H2) was used in flow cytometry on human samples at 1:25 (fig 1b). Cell Transplant (2017) ncbi
mouse monoclonal (BV10A4H2)
  • immunocytochemistry; human; fig 6
In order to elucidate the mechanism by which NHE-1 expression is inhibited by JAK2V617F, BioLegend FLT3 antibody (BioLegend, BV10A4H2) was used in immunocytochemistry on human samples (fig 6). Oncogene (2016) ncbi
Invitrogen
mouse monoclonal (BV10A4H2)
  • flow cytometry; human; loading ...; fig st12
In order to identify new types of human blood dendritic cells, monocytes, and progenitors through single-cell RNA-seq, Invitrogen FLT3 antibody (eBioscience, BV10A4H2) was used in flow cytometry on human samples (fig st12). Science (2017) ncbi
mouse monoclonal (BV10A4H2)
  • flow cytometry; human; 1:10; fig 6
In order to characterize subpopulations of human embryonic stem cells within pluripotent cultures and study their significance, Invitrogen FLT3 antibody (Caltag, MHCD13505) was used in flow cytometry on human samples at 1:10 (fig 6). Stem Cells Dev (2009) ncbi
R&D Systems
mouse monoclonal (66903)
  • flow cytometry; human; loading ...
R&D Systems FLT3 antibody (R&D Systems, FAB812P) was used in flow cytometry on human samples . Clin Transl Immunology (2016) ncbi
goat polyclonal
  • western blot; human
R&D Systems FLT3 antibody (R&D Systems, BAF812) was used in western blot on human samples . Mol Cancer Ther (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (SF1.340)
  • immunoprecipitation; human; loading ...; fig 2
Santa Cruz Biotechnology FLT3 antibody (Santa Cruz Biotechnology, SF-1.340) was used in immunoprecipitation on human samples (fig 2). Iran J Basic Med Sci (2014) ncbi
mouse monoclonal
  • immunoprecipitation; human; loading ...; fig 2
Santa Cruz Biotechnology FLT3 antibody (Santa Cruz Biotechnology, SF-1.340) was used in immunoprecipitation on human samples (fig 2). Iran J Basic Med Sci (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; fig 3a
Cell Signaling Technology FLT3 antibody (Cell Signaling, 3461) was used in western blot on human samples (fig 3a). J Exp Med (2018) ncbi
rabbit monoclonal (30D4)
  • western blot; mouse; loading ...; fig s4f
Cell Signaling Technology FLT3 antibody (Cell Signaling Technology, 3464S) was used in western blot on mouse samples (fig s4f). Nat Med (2018) ncbi
rabbit monoclonal (33G6)
  • western blot; human; loading ...; fig 2d
Cell Signaling Technology FLT3 antibody (Cell Signaling, 3474) was used in western blot on human samples (fig 2d). Leukemia (2017) ncbi
rabbit monoclonal (30D4)
  • western blot; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology FLT3 antibody (Cell Signaling, 3464) was used in western blot on human samples at 1:1000 (fig 2a). Sci Rep (2015) ncbi
mouse monoclonal (54H1)
  • western blot; human; fig 1
Cell Signaling Technology FLT3 antibody (Cell signaling, 3466) was used in western blot on human samples (fig 1). J Cell Biol (2015) ncbi
rabbit monoclonal (8F2)
  • western blot; human; fig 1
Cell Signaling Technology FLT3 antibody (Cell signaling, 3462) was used in western blot on human samples (fig 1). J Cell Biol (2015) ncbi
rabbit monoclonal (8F2)
  • immunoprecipitation; human
Cell Signaling Technology FLT3 antibody (Cell Signaling Technology, 3462) was used in immunoprecipitation on human samples . Mol Cancer Ther (2014) ncbi
BD Biosciences
mouse monoclonal (4G8)
  • flow cytometry; human; loading ...; fig s4c
In order to map the lineage of human dendritic cells, BD Biosciences FLT3 antibody (BD Pharmigen, 4G8) was used in flow cytometry on human samples (fig s4c). Science (2017) ncbi
mouse monoclonal (4G8)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, BD Biosciences FLT3 antibody (BD Pharmingen, 4G8) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (4G8)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences FLT3 antibody (BD, 558996) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (4G8)
  • flow cytometry; human; loading ...; fig s1a, s1b, s1c
In order to characterize oligopotent progenitors from the fetal liver, BD Biosciences FLT3 antibody (BD, 4G8) was used in flow cytometry on human samples (fig s1a, s1b, s1c). Science (2016) ncbi
mouse monoclonal (4G8)
  • flow cytometry; human
BD Biosciences FLT3 antibody (Becton Dickinson, 4G8) was used in flow cytometry on human samples . J Leukoc Biol (2015) ncbi
mouse monoclonal (4G8)
  • flow cytometry; human
BD Biosciences FLT3 antibody (BD, 558996) was used in flow cytometry on human samples . Leukemia (2013) ncbi
Articles Reviewed
  1. Mitchell K, Barreyro L, Todorova T, Taylor S, Antony Debré I, Narayanagari S, et al. IL1RAP potentiates multiple oncogenic signaling pathways in AML. J Exp Med. 2018;215:1709-1727 pubmed publisher
  2. Mathew N, Baumgartner F, Braun L, O Sullivan D, Thomas S, Waterhouse M, et al. Sorafenib promotes graft-versus-leukemia activity in mice and humans through IL-15 production in FLT3-ITD-mutant leukemia cells. Nat Med. 2018;24:282-291 pubmed publisher
  3. Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Hayashi T, Nakachi K, et al. Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors. J Immunol. 2017;199:2777-2793 pubmed publisher
  4. See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  5. Villani A, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017;356: pubmed publisher
  6. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  7. Matsuoka Y, Takahashi M, Sumide K, Kawamura H, Nakatsuka R, Fujioka T, et al. CD34 Antigen and the MPL Receptor Expression Defines a Novel Class of Human Cord Blood-Derived Primitive Hematopoietic Stem Cells. Cell Transplant. 2017;26:1043-1058 pubmed publisher
  8. Wu M, Hamaker M, Li L, Small D, Duffield A. DOCK2 interacts with FLT3 and modulates the survival of FLT3-expressing leukemia cells. Leukemia. 2017;31:688-696 pubmed publisher
  9. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  10. Ohradanova Repic A, Machacek C, Fischer M, Stockinger H. Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel. Clin Transl Immunology. 2016;5:e55 pubmed publisher
  11. Notta F, Zandi S, Takayama N, Dobson S, Gan O, Wilson G, et al. Distinct routes of lineage development reshape the human blood hierarchy across ontogeny. Science. 2016;351:aab2116 pubmed publisher
  12. Ma S, Yang L, Niu T, Cheng C, Zhong L, Zheng M, et al. SKLB-677, an FLT3 and Wnt/β-catenin signaling inhibitor, displays potent activity in models of FLT3-driven AML. Sci Rep. 2015;5:15646 pubmed publisher
  13. Xia H, Najafov A, Geng J, Galan Acosta L, Han X, Guo Y, et al. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. J Cell Biol. 2015;210:705-16 pubmed publisher
  14. Ahn J, Li J, Chen E, Kent D, Park H, Green A. JAK2V617F mediates resistance to DNA damage-induced apoptosis by modulating FOXO3A localization and Bcl-xL deamidation. Oncogene. 2016;35:2235-46 pubmed publisher
  15. Mashkani B, Griffith R, Ashman L. Differences in growth promotion, drug response and intracellular protein trafficking of FLT3 mutants. Iran J Basic Med Sci. 2014;17:867-73 pubmed
  16. Bigley V, McGovern N, Milne P, Dickinson R, Pagan S, Cookson S, et al. Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells. J Leukoc Biol. 2015;97:627-34 pubmed publisher
  17. Keegan K, Li C, Li Z, Ma J, Ragains M, Coberly S, et al. Preclinical evaluation of AMG 925, a FLT3/CDK4 dual kinase inhibitor for treating acute myeloid leukemia. Mol Cancer Ther. 2014;13:880-9 pubmed publisher
  18. Caldarelli A, Muller J, Paskowski Rogacz M, Herrmann K, Bauer R, Koch S, et al. A genome-wide RNAi screen identifies proteins modulating aberrant FLT3-ITD signaling. Leukemia. 2013;27:2301-10 pubmed publisher
  19. King F, Ritner C, Liszewski W, Kwan H, Pedersen A, Leavitt A, et al. Subpopulations of human embryonic stem cells with distinct tissue-specific fates can be selected from pluripotent cultures. Stem Cells Dev. 2009;18:1441-50 pubmed publisher