This is a Validated Antibody Database (VAD) review about human FLIP, based on 17 published articles (read how Labome selects the articles), using FLIP antibody in all methods. It is aimed to help Labome visitors find the most suited FLIP antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FLIP synonym: CASH; CASP8AP1; CLARP; Casper; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; cFLIP

Santa Cruz Biotechnology
mouse monoclonal (G-11)
  • western blot; rat; loading ...; fig 5c
In order to elucidate the regulation and function of enzymatically inactive heparanase, Santa Cruz Biotechnology FLIP antibody (Santa Cruz, sc-5276) was used in western blot on rat samples (fig 5c). Cardiovasc Res (2016) ncbi
mouse monoclonal (G-11)
  • western blot; human; 1:1000; fig 1b
In order to identify the interaction between Calmodulin and cFLIPL, Santa Cruz Biotechnology FLIP antibody (Santa Cruz Biotechnolgy, sc5276) was used in western blot on human samples at 1:1000 (fig 1b). PLoS ONE (2015) ncbi
mouse monoclonal (G-11)
  • western blot; human; 1:200; fig 5
Santa Cruz Biotechnology FLIP antibody (Santa Cruz, sc-5276) was used in western blot on human samples at 1:200 (fig 5). PLoS ONE (2015) ncbi
mouse monoclonal (G-11)
  • western blot; rat; 1:100
Santa Cruz Biotechnology FLIP antibody (Santa Cruz, SC-5276) was used in western blot on rat samples at 1:100. Mol Vis (2014) ncbi
mouse monoclonal (G-11)
  • western blot; human; 1:200
Santa Cruz Biotechnology FLIP antibody (Santa Cruz Biotechnology, sc-5276) was used in western blot on human samples at 1:200. Biomed Res Int (2014) ncbi
mouse monoclonal (G-11)
  • western blot; human; fig 7d
Santa Cruz Biotechnology FLIP antibody (Santa Cruz Biotechnology, sc-5276) was used in western blot on human samples (fig 7d). J Biol Chem (2013) ncbi
mouse monoclonal (G-11)
  • western blot; human
Santa Cruz Biotechnology FLIP antibody (Santa Cruz Biotechnology, G11) was used in western blot on human samples . J Biol Chem (2004) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 3a
  • western blot; mouse; loading ...; fig 3c
Abcam FLIP antibody (Abcam, ab8421) was used in immunohistochemistry on human samples (fig 3a) and in western blot on mouse samples (fig 3c). Cell Death Dis (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig s8
Abcam FLIP antibody (Abcam, ab6144) was used in immunohistochemistry on human samples (fig s8). Cell Death Dis (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; 1:100; fig 6
In order to analyze a Kawasaki disease model and how TNF alpha induces vascular endothelial cells apoptosis through overexpressing pregnancy induced noncoding RNA, Invitrogen FLIP antibody (ThermoFisher, PA5-19899) was used in western blot on human samples at 1:100 (fig 6). Int J Biochem Cell Biol (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D5J1E)
  • western blot; mouse; 1:1000; fig 7n
Cell Signaling Technology FLIP antibody (Cell Signaling, 56343) was used in western blot on mouse samples at 1:1000 (fig 7n). Ann Neurol (2021) ncbi
domestic rabbit monoclonal (D5J1E)
  • western blot; human; 1:1000; loading ...; fig 1f
Cell Signaling Technology FLIP antibody (CST, 56343) was used in western blot on human samples at 1:1000 (fig 1f). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D5J1E)
  • western blot; mouse; loading ...; fig 5e
Cell Signaling Technology FLIP antibody (Cell Signaling, 56343) was used in western blot on mouse samples (fig 5e). Sci Adv (2019) ncbi
domestic rabbit monoclonal (D5J1E)
  • western blot; mouse; 1:1000; loading ...; fig 5a
Cell Signaling Technology FLIP antibody (Cell Signaling Technology, 56343) was used in western blot on mouse samples at 1:1000 (fig 5a). J Cell Sci (2019) ncbi
domestic rabbit monoclonal (D5J1E)
  • western blot; human; loading ...; fig 5c
Cell Signaling Technology FLIP antibody (Cell Signaling, D5J1E) was used in western blot on human samples (fig 5c). Nat Commun (2018) ncbi
domestic rabbit monoclonal (D16A8)
  • western blot; human
In order to study the effect of interleukin 7 on CD95 protein in CD4+ T cells and its mechanism, Cell Signaling Technology FLIP antibody (Cell Signaling Technology, 8510S) was used in western blot on human samples . J Biol Chem (2015) ncbi
domestic rabbit monoclonal (D16A8)
  • western blot; human
Cell Signaling Technology FLIP antibody (Cell Signaling, 8510) was used in western blot on human samples . Apoptosis (2013) ncbi
Articles Reviewed
  1. Chen J, Chen K, Wang L, Luo J, Zheng Q, He Y. Decoy receptor 2 mediates the apoptosis-resistant phenotype of senescent renal tubular cells and accelerates renal fibrosis in diabetic nephropathy. Cell Death Dis. 2022;13:522 pubmed publisher
  2. Villanueva E, Tresse E, Liu Y, Duarte J, Jimenez Duran G, Ejlerskov P, et al. Neuronal TNFα, Not α-Syn, Underlies PDD-Like Disease Progression in IFNβ-KO Mice. Ann Neurol. 2021;90:789-807 pubmed publisher
  3. Supper E, Rudat S, Iyer V, Droop A, Wong K, Spinella J, et al. Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Nat Commun. 2021;12:2482 pubmed publisher
  4. Yang S, Harding A, Sweeney C, Miao D, Swan G, Zhou C, et al. Control of antiviral innate immune response by protein geranylgeranylation. Sci Adv. 2019;5:eaav7999 pubmed publisher
  5. Wang Z, Feng J, Yu J, Chen G. FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation. J Cell Sci. 2019;132: pubmed publisher
  6. Fiore A, Ugel S, De Sanctis F, Sandri S, Fracasso G, Trovato R, et al. Induction of immunosuppressive functions and NF-κB by FLIP in monocytes. Nat Commun. 2018;9:5193 pubmed publisher
  7. Wang F, Jia J, Lal N, Zhang D, Chiu A, Wan A, et al. High glucose facilitated endothelial heparanase transfer to the cardiomyocyte modifies its cell death signature. Cardiovasc Res. 2016;112:656-668 pubmed
  8. Al Lamki R, Lu W, Manalo P, Wang J, Warren A, Tolkovsky A, et al. Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis. Cell Death Dis. 2016;7:e2287 pubmed publisher
  9. Jiang C, Fang X, Jiang Y, Shen F, Hu Z, Li X, et al. TNF-α induces vascular endothelial cells apoptosis through overexpressing pregnancy induced noncoding RNA in Kawasaki disease model. Int J Biochem Cell Biol. 2016;72:118-124 pubmed publisher
  10. Gaidos G, Panaitiu A, Guo B, Pellegrini M, Mierke D. Identification and Characterization of the Interaction Site between cFLIPL and Calmodulin. PLoS ONE. 2015;10:e0141692 pubmed publisher
  11. Braun F, Mathur R, Sehgal L, Wilkie Grantham R, Chandra J, Berkova Z, et al. Inhibition of methyltransferases accelerates degradation of cFLIP and sensitizes B-cell lymphoma cells to TRAIL-induced apoptosis. PLoS ONE. 2015;10:e0117994 pubmed publisher
  12. Yin Y, Zhang S, Luo H, Zhang X, Geng G, Li J, et al. Interleukin 7 up-regulates CD95 protein on CD4+ T cells by affecting mRNA alternative splicing: priming for a synergistic effect on HIV-1 reservoir maintenance. J Biol Chem. 2015;290:35-45 pubmed publisher
  13. Gao S, Andreeva K, Cooper N. Ischemia-reperfusion injury of the retina is linked to necroptosis via the ERK1/2-RIP3 pathway. Mol Vis. 2014;20:1374-87 pubmed
  14. Chondrogiannis G, Kastamoulas M, Kanavaros P, Vartholomatos G, Bai M, Baltogiannis D, et al. Cytokine effects on cell viability and death of prostate carcinoma cells. Biomed Res Int. 2014;2014:536049 pubmed publisher
  15. Bucur O, Pennarun B, Stancu A, Nadler M, Muraru M, Bertomeu T, et al. Poor antibody validation is a challenge in biomedical research: a case study for detection of c-FLIP. Apoptosis. 2013;18:1154-62 pubmed publisher
  16. Wilkie Grantham R, Matsuzawa S, Reed J. Novel phosphorylation and ubiquitination sites regulate reactive oxygen species-dependent degradation of anti-apoptotic c-FLIP protein. J Biol Chem. 2013;288:12777-90 pubmed publisher
  17. Jin T, Kurakin A, Benhaga N, Abe K, Mohseni M, Sandra F, et al. Fas-associated protein with death domain (FADD)-independent recruitment of c-FLIPL to death receptor 5. J Biol Chem. 2004;279:55594-601 pubmed