This is a Validated Antibody Database (VAD) review about human FLG, based on 15 published articles (read how Labome selects the articles), using FLG antibody in all methods. It is aimed to help Labome visitors find the most suited FLG antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FLG synonym: ATOD2

BioLegend
domestic rabbit polyclonal (Poly19058)
  • immunohistochemistry; mouse; loading ...; fig 3a
BioLegend FLG antibody (Covance, PRB-417P) was used in immunohistochemistry on mouse samples (fig 3a). Cell Death Differ (2020) ncbi
domestic rabbit polyclonal (Poly19058)
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 3c
BioLegend FLG antibody (Covance, PRB-417P) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig 3c). J Invest Dermatol (2019) ncbi
domestic rabbit polyclonal (Poly19058)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1n
BioLegend FLG antibody (Covance, PRB-417P) was used in immunohistochemistry on mouse samples at 1:100 (fig 1n). Nature (2018) ncbi
domestic rabbit polyclonal (Poly19058)
  • immunohistochemistry; mouse; loading ...; fig 3b
In order to generate and characterize mice that lack CD44 in the skin, BioLegend FLG antibody (Covance, PRB-417P) was used in immunohistochemistry on mouse samples (fig 3b). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal (Poly19058)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 2D
In order to show that deletion of the gene encoding RAB guanine nucleotide exchange factor 1 in keratinocytes severely impairs epidermal barrier function in mice, BioLegend FLG antibody (Covance, PRB-417P) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 2D). J Clin Invest (2016) ncbi
domestic rabbit polyclonal (Poly19058)
  • western blot; mouse
In order to study functionally distinct gamma-tubulin complexes and divergent regulation during differentiation, BioLegend FLG antibody (Covance, PRB-417P) was used in western blot on mouse samples . J Cell Biol (2016) ncbi
domestic rabbit polyclonal (Poly19058)
  • western blot; mouse; fig 4
In order to analyze the induction of tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo due to loss of keratin 17, BioLegend FLG antibody (Biolegend, PRB-417P) was used in western blot on mouse samples (fig 4). Oncogene (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (AKH1)
  • immunohistochemistry; human; 1:100; loading ...; fig s1-3
Santa Cruz Biotechnology FLG antibody (Santa Cruz Biotechnology, sc-66192) was used in immunohistochemistry on human samples at 1:100 (fig s1-3). elife (2020) ncbi
mouse monoclonal (AKH1)
  • immunohistochemistry; mouse; loading ...; fig 6b
  • immunocytochemistry; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 2c
In order to determine that the transcriptional activities of liver X receptor alpha and beta are activated by raffinose, Santa Cruz Biotechnology FLG antibody (Santa Cruz, sc-66192) was used in immunohistochemistry on mouse samples (fig 6b), in immunocytochemistry on human samples (fig 3a) and in western blot on human samples (fig 2c). Sci Rep (2017) ncbi
mouse monoclonal (AKH1)
  • immunohistochemistry - frozen section; human; 1:100; fig 4
Santa Cruz Biotechnology FLG antibody (SantaCruz, SC-66192) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 4). PLoS ONE (2014) ncbi
Abcam
mouse monoclonal (FLG/1562)
  • immunohistochemistry - frozen section; human; 1:250; loading ...; fig 2b
  • western blot; human; 1:250; loading ...; fig 2c
Abcam FLG antibody (Abcam, ab3137) was used in immunohistochemistry - frozen section on human samples at 1:250 (fig 2b) and in western blot on human samples at 1:250 (fig 2c). J Invest Dermatol (2019) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100; loading ...; fig 2b
In order to investigate the effects of manufacturing protocols and donor sources on the quality of skin models generated from fibroblasts and keratinocytes derived from juvenile foreskins, MilliporeSigma FLG antibody (Sigma-Aldrich, HPA030189) was used in immunohistochemistry on human samples at 1:100 (fig 2b). Biotechnol J (2016) ncbi
Invitrogen
mouse monoclonal (FLG01)
  • immunocytochemistry; human; fig 3
In order to study the function of retinoblastoma protein in the stromal compartment, Invitrogen FLG antibody (Neomarkers, MS 449 P1) was used in immunocytochemistry on human samples (fig 3). J Invest Dermatol (2012) ncbi
Leica Biosystems
monoclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 5
Leica Biosystems FLG antibody (Leica Biosystems, NCL-FLG) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 5). Acta Derm Venereol (2017) ncbi
monoclonal
  • immunohistochemistry; human; 1:200; fig 1a
In order to demonstrate that epidermal equivalents of filaggrin null keratinocytes do not have impaired skin barrier function, Leica Biosystems FLG antibody (Novocastra, NCL-FILAGGRIN) was used in immunohistochemistry on human samples at 1:200 (fig 1a). J Allergy Clin Immunol (2017) ncbi
monoclonal
  • immunohistochemistry - paraffin section; human; 1:150
In order to evaluate the expression of skin barrier proteins and of circulating inflammatory cytokines in adults with atopic dermatitis, Leica Biosystems FLG antibody (Novocastra, NCL-filaggrin) was used in immunohistochemistry - paraffin section on human samples at 1:150. J Eur Acad Dermatol Venereol (2015) ncbi
Articles Reviewed
  1. Pan L, Lemieux M, Thomas T, Rogers J, Lipper C, Lee W, et al. IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiation. elife. 2020;9: pubmed publisher
  2. Sanz Gómez N, de Pedro I, Ortigosa B, Santamaria D, Malumbres M, de Carcer G, et al. Squamous differentiation requires G2/mitosis slippage to avoid apoptosis. Cell Death Differ. 2020;27:2451-2467 pubmed publisher
  3. Suga H, Oka T, Sugaya M, Sato Y, Ishii T, Nishida H, et al. Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption. J Invest Dermatol. 2019;: pubmed publisher
  4. Liakath Ali K, Mills E, Sequeira I, Lichtenberger B, Pisco A, Sipilä K, et al. An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis. Nature. 2018;556:376-380 pubmed publisher
  5. Na T, Kim G, Oh H, Lee M, Han Y, Kim K, et al. The trisaccharide raffinose modulates epidermal differentiation through activation of liver X receptor. Sci Rep. 2017;7:43823 pubmed publisher
  6. Nygaard U, van den Bogaard E, Niehues H, Hvid M, Deleuran M, Johansen C, et al. The "Alarmins" HMBG1 and IL-33 Downregulate Structural Skin Barrier Proteins and Impair Epidermal Growth. Acta Derm Venereol. 2017;97:305-312 pubmed publisher
  7. Shatirishvili M, Burk A, Franz C, Pace G, Kastilan T, Breuhahn K, et al. Epidermal-specific deletion of CD44 reveals a function in keratinocytes in response to mechanical stress. Cell Death Dis. 2016;7:e2461 pubmed publisher
  8. Marichal T, Gaudenzio N, El Abbas S, Sibilano R, Zurek O, Starkl P, et al. Guanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis. J Clin Invest. 2016;126:4497-4515 pubmed publisher
  9. Niehues H, Schalkwijk J, van Vlijmen Willems I, Rodijk Olthuis D, van Rossum M, Wladykowski E, et al. Epidermal equivalents of filaggrin null keratinocytes do not show impaired skin barrier function. J Allergy Clin Immunol. 2017;139:1979-1981.e13 pubmed publisher
  10. Lange J, Weil F, Riegler C, Groeber F, Rebhan S, Kurdyn S, et al. Interactions of donor sources and media influence the histo-morphological quality of full-thickness skin models. Biotechnol J. 2016;11:1352-1361 pubmed publisher
  11. Muroyama A, Seldin L, Lechler T. Divergent regulation of functionally distinct γ-tubulin complexes during differentiation. J Cell Biol. 2016;213:679-92 pubmed publisher
  12. Hobbs R, Batazzi A, Han M, Coulombe P. Loss of Keratin 17 induces tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo. Oncogene. 2016;35:5653-5662 pubmed publisher
  13. Batista D, Perez L, Orfali R, Zaniboni M, Samorano L, Pereira N, et al. Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis. J Eur Acad Dermatol Venereol. 2015;29:1091-5 pubmed publisher
  14. Rabeony H, Petit Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, et al. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1?, TNF? and oncostatin M. PLoS ONE. 2014;9:e101937 pubmed publisher
  15. Pickard A, Cichon A, Menges C, Patel D, McCance D. Regulation of epithelial differentiation and proliferation by the stroma: a role for the retinoblastoma protein. J Invest Dermatol. 2012;132:2691-9 pubmed publisher