This is a Validated Antibody Database (VAD) review about human FLCN, based on 9 published articles (read how Labome selects the articles), using FLCN antibody in all methods. It is aimed to help Labome visitors find the most suited FLCN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FLCN synonym: BHD; FLCL; folliculin; BHD skin lesion fibrofolliculoma protein; birt-Hogg-Dube syndrome protein

Knockout validation
Abcam
rabbit monoclonal (EPNCIR147)
  • western blot knockout validation; mouse; loading ...; fig 1b
Abcam FLCN antibody (Abcam, ab124885) was used in western blot knockout validation on mouse samples (fig 1b). Genes Dev (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D14G9)
  • western blot knockout validation; human; 1:2000; loading ...; fig 2c
Cell Signaling Technology FLCN antibody (CST, D14G9) was used in western blot knockout validation on human samples at 1:2000 (fig 2c). Nat Commun (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-4)
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 1a
In order to show that FLCN regulates mTORC1 by modulating the leucine signal in lysosome, Santa Cruz Biotechnology FLCN antibody (Santa Cruz, 271558) was used in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 1a). PLoS ONE (2016) ncbi
Abcam
rabbit monoclonal (EPNCIR147)
  • western blot knockout validation; mouse; loading ...; fig 1b
Abcam FLCN antibody (Abcam, ab124885) was used in western blot knockout validation on mouse samples (fig 1b). Genes Dev (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D14G9)
  • western blot knockout validation; human; 1:2000; loading ...; fig 2c
Cell Signaling Technology FLCN antibody (CST, D14G9) was used in western blot knockout validation on human samples at 1:2000 (fig 2c). Nat Commun (2019) ncbi
rabbit monoclonal (D14G9)
  • western blot; human; 1:3000; loading ...; fig 3c
Cell Signaling Technology FLCN antibody (Cell Signaling Technology, 3697) was used in western blot on human samples at 1:3000 (fig 3c). Nat Commun (2017) ncbi
rabbit monoclonal (D14G9)
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology FLCN antibody (Cell Signaling, 3697) was used in western blot on human samples (fig 1a). Oncotarget (2017) ncbi
rabbit monoclonal (D14G9)
  • western blot; human; fig s6i
Cell Signaling Technology FLCN antibody (Cell Signaling, 3697) was used in western blot on human samples (fig s6i). Cell (2016) ncbi
rabbit monoclonal (D14G9)
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 1a
In order to show that FLCN regulates mTORC1 by modulating the leucine signal in lysosome, Cell Signaling Technology FLCN antibody (Cell Signalling, 3697) was used in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 1a). PLoS ONE (2016) ncbi
rabbit monoclonal (D14G9)
  • immunocytochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 1c
Cell Signaling Technology FLCN antibody (Cell Signaling, D14G9) was used in immunocytochemistry on human samples (fig 1a) and in western blot on human samples (fig 1c). EMBO Rep (2016) ncbi
rabbit monoclonal (D14G9)
  • western blot; human; 1:2000
Cell Signaling Technology FLCN antibody (Cell Signaling, 3697S) was used in western blot on human samples at 1:2000. Nat Cell Biol (2016) ncbi
rabbit monoclonal (D14G9)
  • immunocytochemistry; human; 1:200
In order to characterize a novel ciliopathy Birt-Hogg-Dube syndrome, Cell Signaling Technology FLCN antibody (Cell Signalling, 3697) was used in immunocytochemistry on human samples at 1:200. Hum Mol Genet (2013) ncbi
Articles Reviewed
  1. Mathieu J, Detraux D, Kuppers D, Wang Y, Cavanaugh C, Sidhu S, et al. Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency. Nat Commun. 2019;10:632 pubmed publisher
  2. Laviolette L, Mermoud J, Calvo I, Olson N, Boukhali M, Steinlein O, et al. Negative regulation of EGFR signalling by the human folliculin tumour suppressor protein. Nat Commun. 2017;8:15866 pubmed publisher
  3. Nagashima K, Fukushima H, Shimizu K, Yamada A, Hidaka M, Hasumi H, et al. Nutrient-induced FNIP degradation by SCFβ-TRCP regulates FLCN complex localization and promotes renal cancer progression. Oncotarget. 2017;8:9947-9960 pubmed publisher
  4. Wu L, Zhou B, Oshiro Rapley N, Li M, Paulo J, Webster C, et al. An Ancient, Unified Mechanism for Metformin Growth Inhibition in C. elegans and Cancer. Cell. 2016;167:1705-1718.e13 pubmed publisher
  5. Wada S, Neinast M, Jang C, Ibrahim Y, Lee G, Babu A, et al. The tumor suppressor FLCN mediates an alternate mTOR pathway to regulate browning of adipose tissue. Genes Dev. 2016;30:2551-2564 pubmed
  6. Wu X, Zhao L, Chen Z, Ji X, Qiao X, Jin Y, et al. FLCN Maintains the Leucine Level in Lysosome to Stimulate mTORC1. PLoS ONE. 2016;11:e0157100 pubmed publisher
  7. Starling G, Yip Y, Sanger A, Morton P, Eden E, Dodding M. Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO Rep. 2016;17:823-41 pubmed publisher
  8. Liu X, Xiao Z, Han L, Zhang J, Lee S, Wang W, et al. LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Nat Cell Biol. 2016;18:431-42 pubmed publisher
  9. Luijten M, Basten S, Claessens T, Vernooij M, Scott C, Janssen R, et al. Birt-Hogg-Dube syndrome is a novel ciliopathy. Hum Mol Genet. 2013;22:4383-97 pubmed publisher