This is a Validated Antibody Database (VAD) review about human FHL2, based on 6 published articles (read how Labome selects the articles), using FHL2 antibody in all methods. It is aimed to help Labome visitors find the most suited FHL2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FHL2 synonym: AAG11; DRAL; FHL-2; SLIM-3; SLIM3; four and a half LIM domains protein 2; LIM domain protein DRAL; aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; skeletal muscle LIM-protein 3

Invitrogen
mouse monoclonal (F4B2-B11)
  • immunocytochemistry; mouse; loading ...; fig 1d
  • western blot; mouse; loading ...; fig 1c
  • immunocytochemistry; human; loading ...; fig 1i
  • western blot; human; loading ...; fig 1f
In order to examine the localization and function of FHL2 in natural killer cells, Invitrogen FHL2 antibody (Thermo Fisher Scientific, F4B2-B11) was used in immunocytochemistry on mouse samples (fig 1d), in western blot on mouse samples (fig 1c), in immunocytochemistry on human samples (fig 1i) and in western blot on human samples (fig 1f). Front Immunol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 3c
  • western blot; human; loading ...; fig 3e
In order to investigate the differentiated vs stem-like properties of cancer cells., Invitrogen FHL2 antibody (ThermoFisher Scientific, PA5-28643) was used in immunocytochemistry on human samples (fig 3c) and in western blot on human samples (fig 3e). Breast Dis (2016) ncbi
Proteintech Group
rabbit polyclonal
  • flow cytometry; human; 1:1000; loading ...; fig 10
  • western blot; human; 1:1000; loading ...; fig 10c
Proteintech Group FHL2 antibody (Proteintech, 21619-1-AP) was used in flow cytometry on human samples at 1:1000 (fig 10) and in western blot on human samples at 1:1000 (fig 10c). Nat Commun (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F4B2-B11)
  • immunoprecipitation; rat; loading ...; fig 4b
  • immunocytochemistry; rat; loading ...; fig 6a
  • western blot; rat; loading ...; fig 5a
In order to report that mammalian actin-binding protein-1 and FHL2 are binding partners that regulate Rho GTPase signaling and breast cancer cell invasion, Santa Cruz Biotechnology FHL2 antibody (Santa Cruz, F4B2-B11) was used in immunoprecipitation on rat samples (fig 4b), in immunocytochemistry on rat samples (fig 6a) and in western blot on rat samples (fig 5a). J Biol Chem (2016) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry; mouse
Abcam FHL2 antibody (Abcam, ab66399) was used in immunohistochemistry on mouse samples . PLoS ONE (2013) ncbi
MBL International
mouse monoclonal (11-134)
  • immunohistochemistry - paraffin section; human; loading ...; fig s9d
  • immunocytochemistry; human; loading ...; fig 1e
  • western blot; human; loading ...; fig 3b
MBL International FHL2 antibody (MBL International, 11-134) was used in immunohistochemistry - paraffin section on human samples (fig s9d), in immunocytochemistry on human samples (fig 1e) and in western blot on human samples (fig 3b). Oncogene (2016) ncbi
Articles Reviewed
  1. Baranek T, Morello E, Valayer A, Aimar R, Bréa D, Henry C, et al. FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection. Front Immunol. 2017;8:123 pubmed publisher
  2. Xu X, Fan Z, Liang C, Li L, Wang L, Liang Y, et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun. 2017;8:14059 pubmed publisher
  3. Jehanno C, Flouriot G, Nicol Benoît F, Le Page Y, Le Goff P, Michel D. Envisioning metastasis as a transdifferentiation phenomenon clarifies discordant results on cancer. Breast Dis. 2016;36:47-59 pubmed publisher
  4. Boateng L, Bennin D, de Oliveira S, Huttenlocher A. Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion. J Biol Chem. 2016;291:13987-98 pubmed publisher
  5. Jin H, Lee K, Kim Y, Oh H, Maeng Y, Kim T, et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene. 2016;35:5106-18 pubmed publisher
  6. Alnajar A, Nordhoff C, Schied T, Chiquet Ehrismann R, Loser K, Vogl T, et al. The LIM-only protein FHL2 attenuates lung inflammation during bleomycin-induced fibrosis. PLoS ONE. 2013;8:e81356 pubmed publisher