This is a Validated Antibody Database (VAD) review about human FERMT2, based on 14 published articles (read how Labome selects the articles), using FERMT2 antibody in all methods. It is aimed to help Labome visitors find the most suited FERMT2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FERMT2 synonym: KIND2; MIG2; PLEKHC1; UNC112; UNC112B; mig-2

Knockout validation
MilliporeSigma
domestic rabbit polyclonal
  • western blot; hamsters; 1:1000; loading ...; fig 1C
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 8A
  • western blot; human; 1:1000; loading ...; fig 8D
In order to establish that Smurf1 controls Kindlin-2 protein levels in cells and hinders integrin activation, MilliporeSigma FERMT2 antibody (Sigma-Aldrich, K3269) was used in western blot on hamsters samples at 1:1000 (fig 1C), in western blot knockout validation on mouse samples at 1:1000 (fig 8A) and in western blot on human samples at 1:1000 (fig 8D). J Cell Biol (2017) ncbi
EMD Millipore
mouse monoclonal (3A3)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 5e
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
  • immunocytochemistry; rat; 1:200; loading ...; fig 3e
  • western blot; rat; loading ...; fig 1c
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunohistochemistry knockout validation on mouse samples (fig 5e), in immunohistochemistry - paraffin section on mouse samples (fig 5e), in immunocytochemistry on rat samples at 1:200 (fig 3e) and in western blot on rat samples (fig 1c). Cell Death Dis (2019) ncbi
EMD Millipore
mouse monoclonal (3A3)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig e2e
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot knockout validation on mouse samples at 1:1000 (fig e2e). Nature (2019) ncbi
EMD Millipore
mouse monoclonal (3A3)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • immunocytochemistry; mouse; 1:500; fig 3
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1) and in immunocytochemistry on mouse samples at 1:500 (fig 3). elife (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:2000; loading ...; fig 3k
  • immunocytochemistry; mouse; 1:50; loading ...; fig 1a
  • western blot; mouse; 1:1000; loading ...; fig 1e
  • western blot; human; 1:1000; loading ...; fig 3i
Abcam FERMT2 antibody (Abcam, ab74030) was used in immunohistochemistry - paraffin section on mouse samples at 1:2000 (fig 3k), in immunocytochemistry on mouse samples at 1:50 (fig 1a), in western blot on mouse samples at 1:1000 (fig 1e) and in western blot on human samples at 1:1000 (fig 3i). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 4c
  • western blot; human; loading ...; fig 1b
Abcam FERMT2 antibody (Abcam, ab74030) was used in immunohistochemistry on human samples (fig 4c) and in western blot on human samples (fig 1b). Aging (Albany NY) (2019) ncbi
OriGene
mouse monoclonal (OTI14A11)
  • immunohistochemistry - paraffin section; human; 1:50; fig 1
In order to assess the prognostic value of an immunohistochemistry-based three-protein signature model in patients with esophageal squamous cell carcinoma, OriGene FERMT2 antibody (Origene, TA500505) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1). Oncotarget (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; hamsters; 1:1000; loading ...; fig 1C
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 8A
  • western blot; human; 1:1000; loading ...; fig 8D
In order to establish that Smurf1 controls Kindlin-2 protein levels in cells and hinders integrin activation, MilliporeSigma FERMT2 antibody (Sigma-Aldrich, K3269) was used in western blot on hamsters samples at 1:1000 (fig 1C), in western blot knockout validation on mouse samples at 1:1000 (fig 8A) and in western blot on human samples at 1:1000 (fig 8D). J Cell Biol (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:250; fig 2
In order to identify five new susceptibility loci for primary angle closure glaucoma through a genome-wide assocation study, MilliporeSigma FERMT2 antibody (Sigma, HPA040505) was used in western blot on human samples at 1:250 (fig 2). Nat Genet (2016) ncbi
EMD Millipore
mouse monoclonal (3A3)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 5e
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
  • immunocytochemistry; rat; 1:200; loading ...; fig 3e
  • western blot; rat; loading ...; fig 1c
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunohistochemistry knockout validation on mouse samples (fig 5e), in immunohistochemistry - paraffin section on mouse samples (fig 5e), in immunocytochemistry on rat samples at 1:200 (fig 3e) and in western blot on rat samples (fig 1c). Cell Death Dis (2019) ncbi
mouse monoclonal (3A3)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig e2e
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot knockout validation on mouse samples at 1:1000 (fig e2e). Nature (2019) ncbi
mouse monoclonal (3A3)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 8B
  • immunocytochemistry; human; 1:200; loading ...; fig 5E
  • western blot; human; 1:1000; loading ...; fig 2A
In order to establish that Smurf1 controls Kindlin-2 protein levels in cells and hinders integrin activation, EMD Millipore FERMT2 antibody (EMD Millipore, Mab2617) was used in immunohistochemistry on mouse samples at 1:200 (fig 8B), in immunocytochemistry on human samples at 1:200 (fig 5E) and in western blot on human samples at 1:1000 (fig 2A). J Cell Biol (2017) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; 1:1000; loading ...; fig s1d
In order to measure the adhesion of alphaV-class and/or alpha5beta1 integrins expressing fibroblasts initiating attachment to fibronectin, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot on mouse samples at 1:1000 (fig s1d). Nat Commun (2017) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; 1:200; fig 3a
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunocytochemistry on human samples at 1:200 (fig 3a). Mol Med Rep (2016) ncbi
mouse monoclonal (3A3)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • immunocytochemistry; mouse; 1:500; fig 3
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1) and in immunocytochemistry on mouse samples at 1:500 (fig 3). elife (2016) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; 1:400; fig 4
In order to describe a role for talin in sensing tissue stiffness, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunocytochemistry on human samples at 1:400 (fig 4). Nat Cell Biol (2015) ncbi
mouse monoclonal (3A3)
  • immunoprecipitation; rat
  • immunocytochemistry; rat
  • western blot; rat
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunoprecipitation on rat samples , in immunocytochemistry on rat samples and in western blot on rat samples . FEBS Lett (2015) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; fig 9
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot on mouse samples (fig 9). Mol Biol Cell (2015) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; fig 5a
EMD Millipore FERMT2 antibody (EMD Millipore, MAB2617) was used in immunocytochemistry on human samples (fig 5a). J Cell Biol (2015) ncbi
Articles Reviewed
  1. Zhu K, Lai Y, Cao H, Bai X, Liu C, Yan Q, et al. Kindlin-2 modulates MafA and β-catenin expression to regulate β-cell function and mass in mice. Nat Commun. 2020;11:484 pubmed publisher
  2. Qi L, Chi X, Zhang X, Feng X, Chu W, Zhang S, et al. Kindlin-2 suppresses transcription factor GATA4 through interaction with SUV39H1 to attenuate hypertrophy. Cell Death Dis. 2019;10:890 pubmed publisher
  3. Wei C, Zhu M, Zhang P, Yang X, Wang L, Ying J, et al. Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma. Aging (Albany NY). 2019;11:6273-6285 pubmed publisher
  4. El Brolosy M, Kontarakis Z, Rossi A, Kuenne C, Günther S, Fukuda N, et al. Genetic compensation triggered by mutant mRNA degradation. Nature. 2019;: pubmed publisher
  5. Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
  6. Bharadwaj M, Strohmeyer N, Colo G, Helenius J, Beerenwinkel N, Schiller H, et al. αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun. 2017;8:14348 pubmed publisher
  7. Zeng F, Xie Y, Liao L, Li L, Chen B, Xie J, et al. Biological characterization of three immortalized esophageal epithelial cell lines. Mol Med Rep. 2016;14:4802-4810 pubmed publisher
  8. Khor C, Do T, Jia H, Nakano M, George R, Abu Amero K, et al. Genome-wide association study identifies five new susceptibility loci for primary angle closure glaucoma. Nat Genet. 2016;48:556-62 pubmed publisher
  9. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  10. Austen K, Ringer P, Mehlich A, Chrostek Grashoff A, Kluger C, Klingner C, et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol. 2015;17:1597-606 pubmed publisher
  11. Qi L, Yu Y, Chi X, Xu W, Lu D, Song Y, et al. Kindlin-2 interacts with α-actinin-2 and β1 integrin to maintain the integrity of the Z-disc in cardiac muscles. FEBS Lett. 2015;589:2155-62 pubmed publisher
  12. Yazlovitskaya E, Tseng H, Viquez O, Tu T, Mernaugh G, McKee K, et al. Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt. Mol Biol Cell. 2015;26:1857-74 pubmed publisher
  13. Artym V, Swatkoski S, Matsumoto K, Campbell C, Petrie R, Dimitriadis E, et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol. 2015;208:331-50 pubmed publisher
  14. Cao H, Zhang S, SHEN J, Wu Z, Wu J, Wang S, et al. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6:5435-48 pubmed