This is a Validated Antibody Database (VAD) review about human FBXO5, based on 9 published articles (read how Labome selects the articles), using FBXO5 antibody in all methods. It is aimed to help Labome visitors find the most suited FBXO5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FBXO5 synonym: EMI1; FBX5; Fbxo31; F-box only protein 5; F-box protein Fbx5; early mitotic inhibitor 1

Invitrogen
mouse monoclonal (3D2D6)
  • western blot; human; 1:500; fig 3
In order to describe a novel irreversible DNA-damage response in antephase, Invitrogen FBXO5 antibody (Novex, 376600) was used in western blot on human samples at 1:500 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (3D2D6)
  • western blot; human; 1:100; fig s6
In order to study APC/C(Cdh1) function, Invitrogen FBXO5 antibody (Thermo Fisher Scientific, 37-6600) was used in western blot on human samples at 1:100 (fig s6). Nucleic Acids Res (2016) ncbi
mouse monoclonal (3D2D6)
  • western blot; human
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, Invitrogen FBXO5 antibody (Zymed, 37-6600) was used in western blot on human samples . Biol Open (2015) ncbi
mouse monoclonal (3D2D6)
  • western blot; human; 1:100
In order to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism, Invitrogen FBXO5 antibody (Invitrogen, 37-6600) was used in western blot on human samples at 1:100. Virology (2013) ncbi
mouse monoclonal (3D2D6)
  • western blot; human; 1:500; fig 1
In order to investigate the regulation and function of APC/C(Cdh1) in non-genotoxic stress responses, Invitrogen FBXO5 antibody (Invitrogen, 37-6600) was used in western blot on human samples at 1:500 (fig 1). PLoS ONE (2012) ncbi
mouse monoclonal (3D2D6)
  • western blot; human; 1:100; fig 1
In order to identify mechanisms other than degradation of Emi1, which release APC/C and promote entry into mitosis, Invitrogen FBXO5 antibody (Invitrogen, 37-6600) was used in western blot on human samples at 1:100 (fig 1). J Biol Chem (2011) ncbi
mouse monoclonal (3D2D6)
  • western blot; human; fig 1
In order to investigate anaphase promoting complex during normal and abnormal mitotic processes, Invitrogen FBXO5 antibody (Zymed, 37-6600) was used in western blot on human samples (fig 1). Cell Cycle (2010) ncbi
mouse monoclonal (3D2D6)
  • western blot; human; fig 1
In order to assess the role of p21(WAF1) in G2-M phase checkpoint control and in prevention of polyploidy after DNA damage, Invitrogen FBXO5 antibody (Neomarkers, 37-6600) was used in western blot on human samples (fig 1). Mol Biol Cell (2009) ncbi
Abcam
rabbit monoclonal (EPR15320-103)
  • western blot; human; 1:1000; loading ...; fig 2a
Abcam FBXO5 antibody (Abcam, ab187144) was used in western blot on human samples at 1:1000 (fig 2a). Biomed Res Int (2018) ncbi
Articles Reviewed
  1. Liu L, Liu K, Yan Y, Chu Z, Tang Y, Tang C. Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells. Biomed Res Int. 2018;2018:7849294 pubmed publisher
  2. Feringa F, Krenning L, Koch A, van den Berg J, van den Broek B, Jalink K, et al. Hypersensitivity to DNA damage in antephase as a safeguard for genome stability. Nat Commun. 2016;7:12618 pubmed publisher
  3. Ercilla A, Llopis A, Feu S, Aranda S, Ernfors P, Freire R, et al. New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress. Nucleic Acids Res. 2016;44:4745-62 pubmed publisher
  4. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  5. Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  6. Chen M, Gutierrez G, Ronai Z. The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress. PLoS ONE. 2012;7:e35520 pubmed publisher
  7. Moshe Y, Bar On O, Ganoth D, Hershko A. Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases. J Biol Chem. 2011;286:16647-57 pubmed publisher
  8. Lee J, Kim J, Margolis R, Fotedar R. Substrate degradation by the anaphase promoting complex occurs during mitotic slippage. Cell Cycle. 2010;9:1792-801 pubmed
  9. Lee J, Kim J, Barbier V, Fotedar A, Fotedar R. DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest. Mol Biol Cell. 2009;20:1891-902 pubmed publisher