This is a Validated Antibody Database (VAD) review about human FAM50A, based on 2 published articles (read how Labome selects the articles), using FAM50A antibody in all methods. It is aimed to help Labome visitors find the most suited FAM50A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FAM50A synonym: 9F; DXS9928E; HXC-26; HXC26; XAP5

Knockout validation
Abcam
domestic rabbit monoclonal (EPR12816)
  • western blot knockout validation; human; 1:3000; loading ...; fig s11b
Abcam FAM50A antibody (ABCAM, ab186410) was used in western blot knockout validation on human samples at 1:3000 (fig s11b). Nat Commun (2021) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...; fig s4a
  • immunohistochemistry knockout validation; zebrafish ; 1:200; loading ...; fig 2a
  • western blot knockout validation; zebrafish ; 1:900; loading ...; fig s6f
  • western blot; human; 1:900; loading ...; fig 6c, s5a
Novus Biologicals FAM50A antibody (Novus, NBP1-89344) was used in immunocytochemistry on mouse samples at 1:200 (fig s4a), in immunohistochemistry knockout validation on zebrafish samples at 1:200 (fig 2a), in western blot knockout validation on zebrafish samples at 1:900 (fig s6f) and in western blot on human samples at 1:900 (fig 6c, s5a). Nat Commun (2020) ncbi
Abcam
domestic rabbit monoclonal (EPR12816)
  • western blot knockout validation; human; 1:3000; loading ...; fig s11b
Abcam FAM50A antibody (ABCAM, ab186410) was used in western blot knockout validation on human samples at 1:3000 (fig s11b). Nat Commun (2021) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...; fig s4a
  • immunohistochemistry knockout validation; zebrafish ; 1:200; loading ...; fig 2a
  • western blot knockout validation; zebrafish ; 1:900; loading ...; fig s6f
  • western blot; human; 1:900; loading ...; fig 6c, s5a
Novus Biologicals FAM50A antibody (Novus, NBP1-89344) was used in immunocytochemistry on mouse samples at 1:200 (fig s4a), in immunohistochemistry knockout validation on zebrafish samples at 1:200 (fig 2a), in western blot knockout validation on zebrafish samples at 1:900 (fig s6f) and in western blot on human samples at 1:900 (fig 6c, s5a). Nat Commun (2020) ncbi
Articles Reviewed
  1. Thompson N, Ranzani M, van der Weyden L, Iyer V, Offord V, Droop A, et al. Combinatorial CRISPR screen identifies fitness effects of gene paralogues. Nat Commun. 2021;12:1302 pubmed publisher
  2. Lee Y, Khan K, Armfield Uhas K, Srikanth S, Thompson N, Pardo M, et al. Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy. Nat Commun. 2020;11:3698 pubmed publisher